FUN12

Summary

Gene Symbol: FUN12
Description: translation initiation factor eIF5B
Alias: yIF2, translation initiation factor eIF5B
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Lee J, Pestova T, Shin B, Cao C, Choi S, Dever T. Initiation factor eIF5B catalyzes second GTP-dependent step in eukaryotic translation initiation. Proc Natl Acad Sci U S A. 2002;99:16689-94 pubmed
    ..Thus, in contrast to bacteria where the single GTPase IF2 is sufficient to catalyze translation initiation, eukaryotic cells require hydrolysis of GTP by both eIF2 and eIF5B to complete translation initiation. ..
  2. Shin B, Kim J, Acker M, Maher K, Lorsch J, Dever T. rRNA suppressor of a eukaryotic translation initiation factor 5B/initiation factor 2 mutant reveals a binding site for translational GTPases on the small ribosomal subunit. Mol Cell Biol. 2009;29:808-21 pubmed publisher
  3. Zheng A, Yu J, Yamamoto R, Ose T, Tanaka I, Yao M. X-ray structures of eIF5B and the eIF5B-eIF1A complex: the conformational flexibility of eIF5B is restricted on the ribosome by interaction with eIF1A. Acta Crystallogr D Biol Crystallogr. 2014;70:3090-8 pubmed publisher
  4. Deng Y, Singer R, Gu W. Translation of ASH1 mRNA is repressed by Puf6p-Fun12p/eIF5B interaction and released by CK2 phosphorylation. Genes Dev. 2008;22:1037-50 pubmed publisher
  5. Shin B, Maag D, Roll Mecak A, Arefin M, Burley S, Lorsch J, et al. Uncoupling of initiation factor eIF5B/IF2 GTPase and translational activities by mutations that lower ribosome affinity. Cell. 2002;111:1015-25 pubmed
    b>Translation initiation factor eIF5B/IF2 is a GTPase that promotes ribosomal subunit joining...
  6. Hiraishi H, Shin B, Udagawa T, Nemoto N, Chowdhury W, Graham J, et al. Interaction between 25S rRNA A loop and eukaryotic translation initiation factor 5B promotes subunit joining and ensures stringent AUG selection. Mol Cell Biol. 2013;33:3540-8 pubmed publisher
    ..This study provides an insight into the effect of ribosomal mutations on translation profiles in eukaryotes...
  7. Farley A, Powell D, Weaver C, Jennings J, Link A. Assessing the components of the eIF3 complex and their phosphorylation status. J Proteome Res. 2011;10:1481-94 pubmed publisher
    ..2-D LC-MS/MS analysis of affinity purified eIF3 complexes showed that several other initiation factors (Fun12, Tif5, Sui3, Pab1, Hcr1, and Sui1) and the casein kinase 2 complex (CK2) copurify...
  8. Jennings M, Pavitt G. eIF5 has GDI activity necessary for translational control by eIF2 phosphorylation. Nature. 2010;465:378-81 pubmed publisher
    ..Together our studies define a new step in the translation initiation pathway, one that is critical for normal translational controls. ..
  9. Qi H, Zakian V. The Saccharomyces telomere-binding protein Cdc13p interacts with both the catalytic subunit of DNA polymerase alpha and the telomerase-associated est1 protein. Genes Dev. 2000;14:1777-88 pubmed
    ..We propose that Cdc13p's interaction with Est1p promotes TG(1-3) strand lengthening by telomerase and its interaction with Pol1p promotes C(1-3)A strand resynthesis by DNA polymerase alpha. ..

More Information

Publications23

  1. Lebaron S, Schneider C, van Nues R, Swiatkowska A, Walsh D, Böttcher B, et al. Proofreading of pre-40S ribosome maturation by a translation initiation factor and 60S subunits. Nat Struct Mol Biol. 2012;19:744-53 pubmed publisher
    ..Nob1 was strongly stimulated by the GTPase activity of Fun12, the yeast homolog of cytoplasmic translation initiation factor eIF5b. Cleavage of the 20S pre-rRNA was also inhibited in vivo and in vitro by blocking binding of Fun12 ..
  2. Olsen D, Savner E, Mathew A, Zhang F, Krishnamoorthy T, Phan L, et al. Domains of eIF1A that mediate binding to eIF2, eIF3 and eIF5B and promote ternary complex recruitment in vivo. EMBO J. 2003;22:193-204 pubmed
    ..We propose a modular organization for eIF1A wherein a core ribosome-binding domain is flanked by flexible segments that mediate interactions with other factors involved in recruitment of TC and release of eIF1A at subunit joining. ..
  3. Fringer J, Acker M, Fekete C, Lorsch J, Dever T. Coupled release of eukaryotic translation initiation factors 5B and 1A from 80S ribosomes following subunit joining. Mol Cell Biol. 2007;27:2384-97 pubmed
    ..Following 80S complex formation, GTP hydrolysis by eIF5B enables the release of both eIF5B and eIF1A, and the ribosome enters the elongation phase of protein synthesis. ..
  4. Choi S, Olsen D, Roll Mecak A, Martung A, Remo K, Burley S, et al. Physical and functional interaction between the eukaryotic orthologs of prokaryotic translation initiation factors IF1 and IF2. Mol Cell Biol. 2000;20:7183-91 pubmed
  5. Baierlein C, Hackmann A, Gross T, Henker L, Hinz F, Krebber H. Monosome formation during translation initiation requires the serine/arginine-rich protein Npl3. Mol Cell Biol. 2013;33:4811-23 pubmed publisher
    ..interactions with the ribosomal subunit joining factors Rpl10 and eukaryotic translation initiation factor 5B/Fun12 and detected a substantially decreased binding of npl3?100 to the Rpl10-containing complex...
  6. Shin B, Acker M, Kim J, Maher K, Arefin S, Lorsch J, et al. Structural integrity of {alpha}-helix H12 in translation initiation factor eIF5B is critical for 80S complex stability. RNA. 2011;17:687-96 pubmed publisher
    b>Translation initiation factor eIF5B promotes GTP-dependent ribosomal subunit joining in the final step of the translation initiation pathway...
  7. Zheng A, Yamamoto R, Sokabe M, Tanaka I, Yao M. Crystallization and preliminary X-ray crystallographic analysis of eIF5B?N and the eIF5B?N-eIF1A?N complex. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011;67:730-3 pubmed publisher
    ..3?Å resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 101.9, b = 120.9, c = 132.8?Å. The asymmetric unit was estimated to contain two complex molecules. ..
  8. ElAntak L, Wagner S, Herrmannová A, Karásková M, Rutkai E, Lukavsky P, et al. The indispensable N-terminal half of eIF3j/HCR1 cooperates with its structurally conserved binding partner eIF3b/PRT1-RRM and with eIF1A in stringent AUG selection. J Mol Biol. 2010;396:1097-116 pubmed publisher
    ..Taken together, we propose that eIF3j/HCR1 closely cooperates with the eIF3b/PRT1 RRM and eIF1A on the ribosome to ensure proper formation of the scanning-arrested conformation required for stringent AUG recognition. ..
  9. Ranjan A, Mizuguchi G, FitzGerald P, Wei D, Wang F, Huang Y, et al. Nucleosome-free region dominates histone acetylation in targeting SWR1 to promoters for H2A.Z replacement. Cell. 2013;154:1232-45 pubmed publisher
  10. Chapa y Lazo B, Ayscough K. Apm4, the mu subunit of yeast AP-2 interacts with Pkc1, and mutation of the Pkc1 consensus phosphorylation site Thr176 inhibits AP-2 recruitment to endocytic sites. Commun Integr Biol. 2014;7:e28522 pubmed publisher
  11. Lee C, Tibbetts A, Kramer G, Appling D. Yeast AEP3p is an accessory factor in initiation of mitochondrial translation. J Biol Chem. 2009;284:34116-25 pubmed publisher
    ..We propose that Aep3p functions as an accessory initiation factor in mitochondrial protein synthesis. ..
  12. Algire M, Maag D, Savio P, Acker M, Tarun S, Sachs A, et al. Development and characterization of a reconstituted yeast translation initiation system. RNA. 2002;8:382-97 pubmed
  13. Choi S, Lee J, Zoll W, Merrick W, Dever T. Promotion of met-tRNAiMet binding to ribosomes by yIF2, a bacterial IF2 homolog in yeast. Science. 1998;280:1757-60 pubmed
    ..Thus, the mechanism of protein synthesis in eukaryotes and prokaryotes is more similar than was previously realized...
  14. Chiu W, Wagner S, Herrmannová A, Burela L, Zhang F, Saini A, et al. The C-terminal region of eukaryotic translation initiation factor 3a (eIF3a) promotes mRNA recruitment, scanning, and, together with eIF3j and the eIF3b RNA recognition motif, selection of AUG start codons. Mol Cell Biol. 2010;30:4415-34 pubmed publisher