Gene Symbol: FPK1
Description: serine/threonine protein kinase FPK1
Alias: serine/threonine protein kinase FPK1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Nakano K, Yamamoto T, Kishimoto T, Noji T, Tanaka K. Protein kinases Fpk1p and Fpk2p are novel regulators of phospholipid asymmetry. Mol Biol Cell. 2008;19:1783-97 pubmed publisher
    ..Herein, we identified a protein kinase gene, FPK1, as a mutation that exhibited synthetic lethality with the cdc50Delta mutation...
  2. Roelants F, Baltz A, TROTT A, Fereres S, Thorner J. A protein kinase network regulates the function of aminophospholipid flippases. Proc Natl Acad Sci U S A. 2010;107:34-9 pubmed publisher
    ..Here we show that a physiologically relevant substrate of Ypk1 is another protein kinase, Fpk1, a known flippase activator...
  3. Niles B, Joslin A, Fresques T, Powers T. TOR complex 2-Ypk1 signaling maintains sphingolipid homeostasis by sensing and regulating ROS accumulation. Cell Rep. 2014;6:541-52 pubmed publisher
    ..Thus, ROS sensing and signaling by TORC2/Ypk1 play a central physiological role in sphingolipid biosynthesis and in the maintenance of cell growth and viability. ..
  4. Yamane Sando Y, Shimobayashi E, Shimobayashi M, Kozutsumi Y, Oka S, Takematsu H. Fpk1/2 kinases regulate cellular sphingoid long-chain base abundance and alter cellular resistance to LCB elevation or depletion. Microbiologyopen. 2014;3:196-212 pubmed publisher
    ..Here, one pair of such DIR (deletion-mediated ISP-1 resistance) genes, FPK1 and FPK2, was further characterized...
  5. Niles B, Powers T. TOR complex 2-Ypk1 signaling regulates actin polarization via reactive oxygen species. Mol Biol Cell. 2014;25:3962-72 pubmed publisher
    ..TORC2-Ypk1 regulates actin polarization both by vacuole-related ROS, controlled by the phospholipid flippase kinase Fpk1 and sphingolipids, and by mitochondria-mediated ROS, controlled by the PKA subunit Tpk3...
  6. Khakhina S, Johnson S, Manoharlal R, Russo S, Blugeon C, Lemoine S, et al. Control of Plasma Membrane Permeability by ABC Transporters. Eukaryot Cell. 2015;14:442-53 pubmed publisher
  7. Rispal D, Eltschinger S, Stahl M, Vaga S, Bodenmiller B, Abraham Y, et al. Target of Rapamycin Complex 2 Regulates Actin Polarization and Endocytosis via Multiple Pathways. J Biol Chem. 2015;290:14963-78 pubmed publisher
    ..The rapid signaling events are mediated in large part through the phospholipid flippase kinases Fpk1 and Fpk2, whereas the slow signaling pathway involves increased plasma membrane tension resulting from a gradual ..
  8. Roelants F, Leskoske K, Pedersen R, Muir A, Liu J, Finnigan G, et al. TOR Complex 2-Regulated Protein Kinase Fpk1 Stimulates Endocytosis via Inhibition of Ark1/Prk1-Related Protein Kinase Akl1 in Saccharomyces cerevisiae. Mol Cell Biol. 2017;37: pubmed publisher
    ..One such substrate is protein kinase Fpk1, which is negatively regulated by Ypk1...
  9. Tang X, Orlicky S, Liu Q, Willems A, Sicheri F, Tyers M. Genome-wide surveys for phosphorylation-dependent substrates of SCF ubiquitin ligases. Methods Enzymol. 2005;399:433-58 pubmed
    ..Both methods have identified novel substrates of Cdc4 and may, in principle, be used to identify numerous new substrates of other SCF and SCF-like complexes from yeast to humans. ..

More Information


  1. Burchett S, Scott A, Errede B, Dohlman H. Identification of novel pheromone-response regulators through systematic overexpression of 120 protein kinases in yeast. J Biol Chem. 2001;276:26472-8 pubmed
    ..These results reveal two new components of the pheromone-signaling cascade in yeast, each acting at a point downstream of the MAPK. ..
  2. Sartorel E, Barrey E, Lau R, Thorner J. Plasma membrane aminoglycerolipid flippase function is required for signaling competence in the yeast mating pheromone response pathway. Mol Biol Cell. 2015;26:134-50 pubmed publisher
    ..responding to α-factor, we found that Dnf1, Dnf2, and Dnf3, as well as the flippase-activating protein kinase Fpk1, localize at the projection ("shmoo") tip where polarized growth is occurring and where Ste5 (the central scaffold ..
  3. Roelants F, Su B, von Wulffen J, Ramachandran S, Sartorel E, TROTT A, et al. Protein kinase Gin4 negatively regulates flippase function and controls plasma membrane asymmetry. J Cell Biol. 2015;208:299-311 pubmed publisher
    ..from the outer to the inner leaflet, stimulated via phosphorylation by cortically localized protein kinase Fpk1. By monitoring Fpk1 activity in vivo, we found that Fpk1 was hyperactive in cells lacking Gin4, a protein kinase ..
  4. Yamagami K, Yamamoto T, Sakai S, Mioka T, Sano T, Igarashi Y, et al. Inositol depletion restores vesicle transport in yeast phospholipid flippase mutants. PLoS ONE. 2015;10:e0120108 pubmed publisher
    ..These results suggest that inositol depletion changes the lipid composition of the endosomal/TGN membranes, which results in vesicle formation from these membranes in the absence of flippases. ..