Gene Symbol: FLO8
Description: Flo8p
Alias: PHD5, STA10, YER108C
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Pan X, Heitman J. Cyclic AMP-dependent protein kinase regulates pseudohyphal differentiation in Saccharomyces cerevisiae. Mol Cell Biol. 1999;19:4874-87 pubmed
    ..PKA signals pseudohyphal growth, in part, by regulating Flo8-dependent expression of the cell surface flocculin Flo11...
  2. La Valle R, Wittenberg C. A role for the Swe1 checkpoint kinase during filamentous growth of Saccharomyces cerevisiae. Genetics. 2001;158:549-62 pubmed
    ..inactivation of Hsl1 is sufficient to suppress the defect in filamentous growth caused by inactivation of Tec1 or Flo8, it is insufficient to promote filamentous growth in the absence of both factors...
  3. Kobayashi O, Yoshimoto H, Sone H. Analysis of the genes activated by the FLO8 gene in Saccharomyces cerevisiae. Curr Genet. 1999;36:256-61 pubmed
    It is thought that the FLO8 gene encodes a transcriptional activator of the dominant flocculation gene FLO1 in Saccharomyces cerevisiae...
  4. Liu H, Styles C, Fink G. Saccharomyces cerevisiae S288C has a mutation in FLO8, a gene required for filamentous growth. Genetics. 1996;144:967-78 pubmed
    ..the standard laboratory strain S288C shows that this defect in dimorphism results from a nonsense mutation in the FLO8 gene. This defect in FLO8 blocks pseudohyphal growth in diploids, haploid invasive growth, and flocculation...
  5. Pan X, Heitman J. Protein kinase A operates a molecular switch that governs yeast pseudohyphal differentiation. Mol Cell Biol. 2002;22:3981-93 pubmed
    ..Here two transcriptional regulators, Flo8 and Sfl1, are shown to be the direct molecular targets of protein kinase A...
  6. Kim T, Kim H, Yoon J, Kang H. Recruitment of the Swi/Snf complex by Ste12-Tec1 promotes Flo8-Mss11-mediated activation of STA1 expression. Mol Cell Biol. 2004;24:9542-56 pubmed
    ..of the STA1 gene, which encodes an extracellular glucoamylase, is activated by the specific DNA-binding activators Flo8, Mss11, Ste12, and Tec1 and the Swi/Snf chromatin-remodeling complex...
  7. Rupp S, Summers E, Lo H, Madhani H, Fink G. MAP kinase and cAMP filamentation signaling pathways converge on the unusually large promoter of the yeast FLO11 gene. EMBO J. 1999;18:1257-69 pubmed
    ..Secondly, mutations in either pathway block FLO11 transcription. Overexpression of STE12 can suppress the loss of FLO8, and overexpression of FLO8 can suppress the loss of STE12...
  8. González A, Casado C, Petrezselyova S, Ruiz A, Arino J. Molecular analysis of a conditional hal3 vhs3 yeast mutant links potassium homeostasis with flocculation and invasiveness. Fungal Genet Biol. 2013;53:1-9 pubmed publisher
    ..Cells lacking Trk1,2 display an invasive phenotype that is abolished by deletion of FLO8 or by increasing the potassium concentration in the medium...
  9. Furukawa K, Sidoux Walter F, Hohmann S. Expression of the yeast aquaporin Aqy2 affects cell surface properties under the control of osmoregulatory and morphogenic signalling pathways. Mol Microbiol. 2009;74:1272-86 pubmed publisher
    ..Our observations suggest a potential link between aquaporins and cell surface properties, and relate to the proposed role of mammalian aquaporins in tumour cell migration and invasion. ..

More Information


  1. Bester M, Pretorius I, Bauer F. The regulation of Saccharomyces cerevisiae FLO gene expression and Ca2+ -dependent flocculation by Flo8p and Mss11p. Curr Genet. 2006;49:375-83 pubmed
    ..The data clearly show that several essential transcription factors are shared by at least two flocculation genes that control different adhesion phenotypes. ..
  2. Li J, Wang L, Wu X, Fang O, Wang L, Lu C, et al. Polygenic molecular architecture underlying non-sexual cell aggregation in budding yeast. DNA Res. 2013;20:55-66 pubmed publisher
    ..with knockout and allele replacement experiments resolved the QTL into the underlying genes (AMN1, RGA1, FLO1, and FLO8) or even into the causative nucleotide...
  3. Barrales R, Jimenez J, Ibeas J. Identification of novel activation mechanisms for FLO11 regulation in Saccharomyces cerevisiae. Genetics. 2008;178:145-56 pubmed publisher
    ..In addition to previously characterized activators, we identified a number of novel FLO11 activators, which reveal the pH response pathway and chromatin-remodeling complexes as central elements involved in FLO11 activation. ..
  4. van Dyk D, Pretorius I, Bauer F. Mss11p is a central element of the regulatory network that controls FLO11 expression and invasive growth in Saccharomyces cerevisiae. Genetics. 2005;169:91-106 pubmed
    ..Taken together, the data strongly suggest a central role for Mss11p in the regulatory network controlling FLO11 expression, invasive growth, and pseudohyphal differentiation. ..
  5. Kim T, Ahn J, Yoon J, Kang H. STA10 repression of STA gene expression is caused by a defective activator, flo8, in Saccharomyces cerevisiae. Curr Genet. 2003;44:261-7 pubmed
    ..most laboratory Saccharomyces cerevisiae strains, which are believed to contain an undefined repressor, designated STA10. To identify the regulator involved in STA10 repression, we investigate the FLO8, MSN1, MSS11, STE12, and TEC1 ..
  6. Kim H, Lee S, Kang H, Oh G, Kim T. Two distinct domains of Flo8 activator mediates its role in transcriptional activation and the physical interaction with Mss11. Biochem Biophys Res Commun. 2014;449:202-7 pubmed publisher
    b>Flo8 is a transcriptional activator essential for the inducible expression of a set of target genes such as STA1, FLO11, and FLO1 encoding an extracellular glucoamylase and two cell surface proteins, respectively...
  7. Reynolds T, Fink G. Bakers' yeast, a model for fungal biofilm formation. Science. 2001;291:878-81 pubmed
    ..The ability to study biofilm formation in a tractable genetic system may facilitate the identification of new targets for antifungal therapy. ..
  8. King L, Butler G. Ace2p, a regulator of CTS1 (chitinase) expression, affects pseudohyphal production in Saccharomyces cerevisiae. Curr Genet. 1998;34:183-91 pubmed
    ..Most yeasts capable of forming pseudohyphae possess a functional FLO8 gene...
  9. Barrales R, Korber P, Jimenez J, Ibeas J. Chromatin modulation at the FLO11 promoter of Saccharomyces cerevisiae by HDAC and Swi/Snf complexes. Genetics. 2012;191:791-803 pubmed publisher
    ..We show that the regulation of chromatin structure represents an important layer of control in the highly complex regulation of the FLO11 promoter. ..
  10. Tobe B, Kitazono A, Garcia J, Gerber R, Bevis B, Choy J, et al. Morphogenesis signaling components influence cell cycle regulation by cyclin dependent kinase. Cell Div. 2009;4:12 pubmed publisher
    ..The dynamic balance between Cks1- and Swe1-dependent regulation of Cdc28 and, thereby, the timing of mitosis during yeast dimorphism is regulated in part by Ras2/cAMP-mediated PKA signaling, a key pathway controlling filamentous growth. ..
  11. Law M, Ciccaglione K. Fine-tuning of histone H3 Lys4 methylation during pseudohyphal differentiation by the CDK submodule of RNA polymerase II. Genetics. 2015;199:435-53 pubmed publisher
    ..Interestingly, deleting known FLO11 activators including FLO8, MSS11, MFG1, TEC1, SNF1, KSS1, and GCN4 results in a range of phenotypic suppression...
  12. Ryan O, Shapiro R, Kurat C, Mayhew D, Baryshnikova A, Chin B, et al. Global gene deletion analysis exploring yeast filamentous growth. Science. 2012;337:1353-6 pubmed publisher
    ..roles in filamentous growth, including MFG1 (YDL233w), whose product binds two morphogenetic transcription factors, Flo8 and Mss11, and functions as a critical transcriptional regulator of filamentous growth in both S. cerevisiae and C...
  13. Gagiano M, van Dyk D, Bauer F, Lambrechts M, Pretorius I. Divergent regulation of the evolutionarily closely related promoters of the Saccharomyces cerevisiae STA2 and MUC1 genes. J Bacteriol. 1999;181:6497-508 pubmed
    ..We also identified the STA10 repressive effect as the absence of Flo8p, a transcriptional activator of flocculation genes in S. cerevisiae.
  14. Wojda I, Bebelman J, Jakubowicz T, Siderius M. Thermosensitivity of the Saccharomyces cerevisiae gpp1gpp2 double deletion strain can be reduced by overexpression of genes involved in cell wall maintenance. Arch Microbiol. 2007;188:175-84 pubmed
    ..Sensitivity to Zymolyase was rescued by overexpression of SSD1, while CFW sensitivity was rescued by SSD1, FLO8 and WSC3-genes isolated as multicopy suppressors of the gpp1gpp2 ts phenotype...
  15. Mao X, Nie X, Cao F, Chen J. Functional analysis of ScSwi1 and CaSwi1 in invasive and pseudohyphal growth of Saccharomyces cerevisiae. Acta Biochim Biophys Sin (Shanghai). 2009;41:594-602 pubmed
    ..We also showed that overproducing Swi1 could suppress the defect of flo8 cells in pseudohyphal growth in diploids, but not invasive growth in haploids...
  16. Matsuzawa T, Morita T, Tanaka N, Tohda H, Takegawa K. Identification of a galactose-specific flocculin essential for non-sexual flocculation and filamentous growth in Schizosaccharomyces pombe. Mol Microbiol. 2011;82:1531-44 pubmed publisher
    ..In the budding yeast Saccharomyces cerevisiae, the transcription factor Flo8 controls expression of some of the genes involved in non-sexual flocculation...
  17. Chen R, Thorner J. Systematic epistasis analysis of the contributions of protein kinase A- and mitogen-activated protein kinase-dependent signaling to nutrient limitation-evoked responses in the yeast Saccharomyces cerevisiae. Genetics. 2010;185:855-70 pubmed publisher
    ..Thus, although there are similarities between haploids and diploids, cell type-specific differences clearly alter the balance of the signaling inputs required to elicit the various nutrient limitation-evoked cellular behaviors. ..
  18. Du Z, Zhang Y, Li L. The Yeast Prion [SWI(+)] Abolishes Multicellular Growth by Triggering Conformational Changes of Multiple Regulators Required for Flocculin Gene Expression. Cell Rep. 2015;13:2865-78 pubmed publisher