Research TopicsGenomes and Genes | FET3SummaryGene Symbol: FET3 Description: ferroxidase FET3 Alias: ferroxidase FET3 Species: Saccharomyces cerevisiae S288c Top Publications
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Publications
- Xue Y, Schmollinger S, Attar N, Campos O, Vogelauer M, Carey M, et al. Endoplasmic reticulum-mitochondria junction is required for iron homeostasis. J Biol Chem. 2017;292:13197-13204 pubmed publisher..Our findings reveal that proper communication between the ER and mitochondria is required for appropriate maintenance of cellular iron levels. ..
- Xiong H, Kobayashi T, Kakei Y, Senoura T, Nakazono M, Takahashi H, et al. AhNRAMP1 iron transporter is involved in iron acquisition in peanut. J Exp Bot. 2012;63:4437-46 pubmed publisher..Induced expression of AhNRAMP1 in tobacco conferred enhanced tolerance to iron deprivation. These results suggest that the AhNRAMP1 is possibly involved in iron acquisition in peanut plants. ..
- Shi X, Stoj C, Romeo A, Kosman D, Zhu Z. Fre1p Cu2+ reduction and Fet3p Cu1+ oxidation modulate copper toxicity in Saccharomyces cerevisiae. J Biol Chem. 2003;278:50309-15 pubmed..Deletion of FET3 leads to iron deficiency; this deletion also causes a copper sensitivity not seen in wild type...
- Stearman R, Yuan D, Yamaguchi Iwai Y, Klausner R, Dancis A. A permease-oxidase complex involved in high-affinity iron uptake in yeast. Science. 1996;271:1552-7 pubmed..Two proteins in the plasma membrane of yeast--a multicopper oxidase, encoded by the FET3 gene, and a permease, encoded by the FTR1 gene--were shown to mediate high-affinity iron uptake...
- De Freitas J, Liba A, Meneghini R, Valentine J, Gralla E. Yeast lacking Cu-Zn superoxide dismutase show altered iron homeostasis. Role of oxidative stress in iron metabolism. J Biol Chem. 2000;275:11645-9 pubmed..Transcription of the high affinity iron transporter gene, FET3, was enhanced in the sod1 mutant, suggesting that iron transport systems were up-regulated...
- Li L, Vulpe C, Kaplan J. Functional studies of hephaestin in yeast: evidence for multicopper oxidase activity in the endocytic pathway. Biochem J. 2003;375:793-8 pubmed..Yeast with a deletion in FET3, which encodes a cell-surface multicopper oxidase, cannot grow on low-iron media...
- de Silva D, Davis Kaplan S, Fergestad J, Kaplan J. Purification and characterization of Fet3 protein, a yeast homologue of ceruloplasmin. J Biol Chem. 1997;272:14208-13 pubmedThe FET3 gene product of Saccharomyces cerevisiae is an essential component of the high affinity iron transport system...
- Yun C, Ferea T, Rashford J, Ardon O, Brown P, Botstein D, et al. Desferrioxamine-mediated iron uptake in Saccharomyces cerevisiae. Evidence for two pathways of iron uptake. J Biol Chem. 2000;275:10709-15 pubmed..Deletion of ARN3 did not prevent yeast from using ferrioxamine B as an iron source; however, deletion of ARN3 and FET3, a component of the high affinity ferrous iron transport system, did prevent uptake of ferrioxamine-bound iron and ..
- Stoj C, Augustine A, Zeigler L, Solomon E, Kosman D. Structural basis of the ferrous iron specificity of the yeast ferroxidase, Fet3p. Biochemistry. 2006;45:12741-9 pubmed..Indeed, this double mutant functions kinetically better as a laccase, albeit a relatively inefficient one. ..
- Protchenko O, Rodriguez Suarez R, Androphy R, Bussey H, Philpott C. A screen for genes of heme uptake identifies the FLC family required for import of FAD into the endoplasmic reticulum. J Biol Chem. 2006;281:21445-57 pubmed..We propose that the FLC genes are required for import of FAD into the lumen of the endoplasmic reticulum, where it is required for disulfide bond formation. ..
- Sedlak E, Ziegler L, Kosman D, Wittung Stafshede P. In vitro unfolding of yeast multicopper oxidase Fet3p variants reveals unique role of each metal site. Proc Natl Acad Sci U S A. 2008;105:19258-63 pubmed publisher..Our results suggest that there is a metal-induced tradeoff between overall protein stability and metal coordination in members of the MCO family. ..
- Strachotová D, Holoubek A, Kucerova H, Benda A, Humpolickova J, Vachova L, et al. Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM). Biochim Biophys Acta. 2012;1818:2126-34 pubmed publisher..In addition, using specially prepared strains, we were able to detect an interaction between molecules of the same Ato protein, namely Ato1p-Ato1p and Ato3p-Ato3p, but not Ato2p-Ato2p. ..
- Batista Nascimento L, Toledano M, Thiele D, Rodrigues Pousada C. Yeast protective response to arsenate involves the repression of the high affinity iron uptake system. Biochim Biophys Acta. 2013;1833:997-1005 pubmed publisher..iron responsive transcription factor, Aft1, as a consequence of a defective high-affinity iron uptake mediated by Fet3 and Ftr1, whose mRNAs are drastically decreased upon arsenic exposure...
- Kupchak B, Garitaonandia I, Villa N, Mullen M, Weaver M, Regalla L, et al. Probing the mechanism of FET3 repression by Izh2p overexpression. Biochim Biophys Acta. 2007;1773:1124-32 pubmed..we show that the Nrg1p/Nrg2p and Msn2p/Msn4p pairs are epistatic to each other with respect to their effects on FET3 expression...
- Pozdnyakova I, Wittung Stafshede P. Non-linear effects of macromolecular crowding on enzymatic activity of multi-copper oxidase. Biochim Biophys Acta. 2010;1804:740-4 pubmed publisher..The observations are compatible with ordering of a non-optimal substrate-binding site and restricted internal dynamics as a result of excluded volume effects making the protein less structurally 'strained'. ..
- Hassett R, Yuan D, Kosman D. Spectral and kinetic properties of the Fet3 protein from Saccharomyces cerevisiae, a multinuclear copper ferroxidase enzyme. J Biol Chem. 1998;273:23274-82 pubmed..The Km values for Fe(II) and O2 were 4.8 and 1.3 microM, respectively, while kcat values for Fe(II) and O2 turnover were 9.5 and 2.3 min-1, consistent with an Fe(II):O2 reaction stoichiometry of 4:1. ..
- Spizzo T, Byersdorfer C, Duesterhoeft S, Eide D. The yeast FET5 gene encodes a FET3-related multicopper oxidase implicated in iron transport. Mol Gen Genet. 1997;256:547-56 pubmedThe yeast FET3 gene encodes an integral membrane multicopper oxidase required for high-affinity iron uptake. The FET4 gene encodes an Fe(II) transporter required for low-affinity uptake...
- Sanvisens N, Romero A, An X, Zhang C, de Llanos R, Martínez Pastor M, et al. Yeast Dun1 kinase regulates ribonucleotide reductase inhibitor Sml1 in response to iron deficiency. Mol Cell Biol. 2014;34:3259-71 pubmed publisher..by Dun1 under low-Fe conditions is highlighted by the synthetic growth defect observed between dun1? and fet3? fet4? mutants, which is rescued by SML1 deletion...
- Singh A, Severance S, Kaur N, Wiltsie W, Kosman D. Assembly, activation, and trafficking of the Fet3p.Ftr1p high affinity iron permease complex in Saccharomyces cerevisiae. J Biol Chem. 2006;281:13355-64 pubmed..The results are consistent with a (minimal) heterodimer model of the Fet3p.Ftr1p complex that supports the trafficking of iron from Fet3p to Ftr1p for iron permeation across the yeast PM. ..
- Stoj C, Augustine A, Solomon E, Kosman D. Structure-function analysis of the cuprous oxidase activity in Fet3p from Saccharomyces cerevisiae. J Biol Chem. 2007;282:7862-8 pubmedThe Fet3 protein from Saccharomyces cerevisiae is a multicopper oxidase with specificity toward Fe(II) and Cu(I)...