Gene Symbol: FCP1
Description: protein serine/threonine phosphatase
Alias: protein serine/threonine phosphatase
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Murray S, Udupa R, Yao S, Hartzog G, Prelich G. Phosphorylation of the RNA polymerase II carboxy-terminal domain by the Bur1 cyclin-dependent kinase. Mol Cell Biol. 2001;21:4089-96 pubmed
    ..These results identify Bur1 as a fourth S. cerevisiae CTD kinase and provide striking functional similarities between Bur1 and metazoan P-TEFb. ..
  2. Costa P, Arndt K. Synthetic lethal interactions suggest a role for the Saccharomyces cerevisiae Rtf1 protein in transcription elongation. Genetics. 2000;156:535-47 pubmed
    ..The screen uncovered mutations in SRB5, CTK1, FCP1, and POB3...
  3. Gudipati R, Villa T, Boulay J, Libri D. Phosphorylation of the RNA polymerase II C-terminal domain dictates transcription termination choice. Nat Struct Mol Biol. 2008;15:786-94 pubmed publisher
    ..We discuss the mechanism underlying the regulation of coexisting cryptic and mRNA-productive transcription. ..
  4. Wu X, Chang A, Sudol M, Hanes S. Genetic interactions between the ESS1 prolyl-isomerase and the RSP5 ubiquitin ligase reveal opposing effects on RNA polymerase II function. Curr Genet. 2001;40:234-42 pubmed
    ..The results suggest a model in which Essl and Rsp5 act opposingly on pol II function to control the level of pol II available for transcription. ..
  5. Smith Kinnaman W, Berna M, Hunter G, True J, Hsu P, Cabello G, et al. The interactome of the atypical phosphatase Rtr1 in Saccharomyces cerevisiae. Mol Biosyst. 2014;10:1730-41 pubmed publisher
    ..Interestingly, we found that the interaction between Rtr1 and RNAPII is decreased in ctk1? strains. We hypothesize that serine-2 CTD phosphorylation is required for Rtr1 recruitment to RNAPII during transcription elongation. ..
  6. Suh M, Ye P, Zhang M, Hausmann S, Shuman S, Gnatt A, et al. Fcp1 directly recognizes the C-terminal domain (CTD) and interacts with a site on RNA polymerase II distinct from the CTD. Proc Natl Acad Sci U S A. 2005;102:17314-9 pubmed
    b>Fcp1 is an essential protein phosphatase that hydrolyzes phosphoserines within the C-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II)...
  7. Pierstorff E, Kane C. Genetic interactions between an RNA polymerase II phosphatase and centromeric elements in Saccharomyces cerevisiae. Mol Genet Genomics. 2004;271:603-15 pubmed
    The Saccharomyces cerevisiase protein phosphatase Fcp1 has been implicated in the regulation of transcription by RNA polymerase II, and is encoded by the essential gene FCP1...
  8. Squazzo S, Costa P, Lindstrom D, Kumer K, Simic R, Jennings J, et al. The Paf1 complex physically and functionally associates with transcription elongation factors in vivo. EMBO J. 2002;21:1764-74 pubmed
    ..Taken together, these data suggest that the Paf1 complex functions during the elongation phase of transcription in conjunction with Spt4-Spt5 and Spt16-Pob3. ..
  9. Ganem C, Devaux F, Torchet C, Jacq C, Quevillon Cheruel S, Labesse G, et al. Ssu72 is a phosphatase essential for transcription termination of snoRNAs and specific mRNAs in yeast. EMBO J. 2003;22:1588-98 pubmed
    ..phosphatase that physically interacts with the CTD kinase Kin28 and functionally interacts with the CTD phosphatase Fcp1. A genome-wide expression analysis of mutant ssu72-ts69 during growth in complete medium revealed a number of ..

More Information


  1. Kamenski T, Heilmeier S, Meinhart A, Cramer P. Structure and mechanism of RNA polymerase II CTD phosphatases. Mol Cell. 2004;15:399-407 pubmed
    ..II) after transcription requires dephosphorylation of the polymerase C-terminal domain (CTD) by the phosphatase Fcp1. We report the X-ray structure of the small CTD phosphatase Scp1, which is homologous to the Fcp1 catalytic domain...
  2. Kilpatrick A, Koharudin L, Calero G, Gronenborn A. Structural and binding studies of the C-terminal domains of yeast TFIIF subunits Tfg1 and Tfg2. Proteins. 2012;80:519-29 pubmed publisher
    ..Tfg1-CTD, but not Tfg2-CTD, also binds to yeast FCP1, an RNA polymerase II-specific phosphatase, and we delineated the interaction surface with the CTD of FCP1...
  3. Kubicek K, Cerná H, Holub P, Pasulka J, Hrossova D, Loehr F, et al. Serine phosphorylation and proline isomerization in RNAP II CTD control recruitment of Nrd1. Genes Dev. 2012;26:1891-6 pubmed publisher
    ..These findings underpin the interplay between covalent and noncovalent changes in the CTD structure that constitute the CTD code. ..
  4. Shohat Tal A, Eshel D. Cell cycle regulators interact with pathways that modulate microtubule stability in Saccharomyces cerevisiae. Eukaryot Cell. 2011;10:1705-13 pubmed publisher
    ..Our results introduce novel roles in microtubule stability for genes whose participation in the process may be masked under normal conditions yet nonetheless acquire a dominant role when microtubule stability is compromised. ..
  5. Arevalo Rodriguez M, Cardenas M, Wu X, Hanes S, Heitman J. Cyclophilin A and Ess1 interact with and regulate silencing by the Sin3-Rpd3 histone deacetylase. EMBO J. 2000;19:3739-49 pubmed
    ..Our findings support a model in which Ess1 and cyclophilin A modulate the activity of the Sin3-Rpd3 complex, and excess histone deacetylation causes mitotic arrest in ess1 mutants. ..
  6. Allepuz Fuster P, Martínez Fernández V, Garrido Godino A, Alonso Aguado S, Hanes S, Navarro F, et al. Rpb4/7 facilitates RNA polymerase II CTD dephosphorylation. Nucleic Acids Res. 2014;42:13674-88 pubmed
    ..RPB4 interacted genetically with genes encoding CTD phosphatases (SSU72, FCP1), CTD kinases (KIN28, CTK1, SRB10) and a prolyl isomerase that targets the CTD (ESS1)...
  7. Mandal S, Cho H, Kim S, Cabane K, Reinberg D. FCP1, a phosphatase specific for the heptapeptide repeat of the largest subunit of RNA polymerase II, stimulates transcription elongation. Mol Cell Biol. 2002;22:7543-52 pubmed
    b>FCP1, a phosphatase specific for the carboxy-terminal domain of RNA polymerase II (RNAP II), was found to stimulate transcript elongation by RNAP II in vitro and in vivo...
  8. Kops O, Zhou X, Lu K. Pin1 modulates the dephosphorylation of the RNA polymerase II C-terminal domain by yeast Fcp1. FEBS Lett. 2002;513:305-11 pubmed
    ..Here we confirm that the CTD phosphatase Fcp1 can suppress a Pin1 mutation in yeast...
  9. Wu X, Wilcox C, Devasahayam G, Hackett R, Ar valo Rodr guez M, Cardenas M, et al. The Ess1 prolyl isomerase is linked to chromatin remodeling complexes and the general transcription machinery. EMBO J. 2000;19:3727-38 pubmed publisher
    ..In this model, Ess1 binds and isomerizes the CTD of RNA polymerase II, thus altering its interaction with proteins required for transcription of essential cell cycle genes...
  10. Calvo O, Manley J. The transcriptional coactivator PC4/Sub1 has multiple functions in RNA polymerase II transcription. EMBO J. 2005;24:1009-20 pubmed
    ..between SUB1 and genes encoding an RNA polymerase II (RNAP II)-specific kinase (KIN28) and phosphatase (FCP1) suggest that Sub1 influences and/or is sensitive to the phosphorylation status of elongating RNAP II...
  11. Kobor M, Archambault J, Lester W, Holstege F, Gileadi O, Jansma D, et al. An unusual eukaryotic protein phosphatase required for transcription by RNA polymerase II and CTD dephosphorylation in S. cerevisiae. Mol Cell. 1999;4:55-62 pubmed
    ..We show here that the essential FCP1 gene of S...
  12. Kobor M, Simon L, Omichinski J, Zhong G, Archambault J, Greenblatt J. A motif shared by TFIIF and TFIIB mediates their interaction with the RNA polymerase II carboxy-terminal domain phosphatase Fcp1p in Saccharomyces cerevisiae. Mol Cell Biol. 2000;20:7438-49 pubmed
    ..motif KEFGK in the RAP74 (Tfg1p) subunit of TFIIF to EEFGE led to both synthetic phenotypes in certain fcp1 tfg1 double mutants and a reduced ability of Fcp1p to activate transcription when it is artificially tethered to a ..
  13. Lindstrom D, Hartzog G. Genetic interactions of Spt4-Spt5 and TFIIS with the RNA polymerase II CTD and CTD modifying enzymes in Saccharomyces cerevisiae. Genetics. 2001;159:487-97 pubmed
    ..or alter the composition of the CTD; affect the Kin28, Bur1, or Ctk1 CTD kinases; and affect the CTD phosphatase Fcp1. We show that Spt5 co-immunoprecipitates with RNA polymerase II that has either a hyper- or a hypophosphorylated ..
  14. Jimeno González S, Schmid M, Malagon F, Haaning L, Jensen T. Rat1p maintains RNA polymerase II CTD phosphorylation balance. RNA. 2014;20:551-8 pubmed publisher
    ..Thus, Rat1p plays more complex roles in controlling transcription than previously thought. ..
  15. Reyes Reyes M, Hampsey M. Role for the Ssu72 C-terminal domain phosphatase in RNA polymerase II transcription elongation. Mol Cell Biol. 2007;27:926-36 pubmed
  16. Archambault J, Chambers R, Kobor M, Ho Y, Cartier M, Bolotin D, et al. An essential component of a C-terminal domain phosphatase that interacts with transcription factor IIF in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1997;94:14300-5 pubmed
    ..II (RPII) large subunit C-terminal domain (CTD) is a novel polypeptide encoded by an essential gene termed FCP1. The Fcp1 protein is localized to the nucleus, and it binds the largest subunit of the yeast general transcription ..
  17. Ghosh A, Shuman S, Lima C. The structure of Fcp1, an essential RNA polymerase II CTD phosphatase. Mol Cell. 2008;32:478-90 pubmed publisher
    ..b>Fcp1 is an essential CTD phosphatase that preferentially hydrolyzes Ser2-PO(4) of the tandem YSPTSPS CTD heptad array...