FAB1

Summary

Gene Symbol: FAB1
Description: 1-phosphatidylinositol-3-phosphate 5-kinase
Alias: SVL7, 1-phosphatidylinositol-3-phosphate 5-kinase
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Efe J, Botelho R, Emr S. Atg18 regulates organelle morphology and Fab1 kinase activity independent of its membrane recruitment by phosphatidylinositol 3,5-bisphosphate. Mol Biol Cell. 2007;18:4232-44 pubmed
    The lipid kinase Fab1 governs yeast vacuole homeostasis by generating PtdIns(3,5)P(2) on the vacuolar membrane. Recruitment of effector proteins by the phospholipid ensures precise regulation of vacuole morphology and function...
  2. Burd C, Emr S. Phosphatidylinositol(3)-phosphate signaling mediated by specific binding to RING FYVE domains. Mol Cell. 1998;2:157-62 pubmed
    ..Our data establish a molecular link between Vps34 PI(3)K and several FYVE domain-containing proteins (Vac1p, Vps27p) required for vacuolar/lysosomal protein trafficking. ..
  3. Cooke F, Dove S, McEwen R, Painter G, Holmes A, Hall M, et al. The stress-activated phosphatidylinositol 3-phosphate 5-kinase Fab1p is essential for vacuole function in S. cerevisiae. Curr Biol. 1998;8:1219-22 pubmed
    ..A search of the Saccharomyces cerevisiae genome database identified FAB1, a gene encoding a PIP kinase homologue and potential PI(3)P 5-kinase...
  4. Bonangelino C, Nau J, Duex J, Brinkman M, Wurmser A, Gary J, et al. Osmotic stress-induced increase of phosphatidylinositol 3,5-bisphosphate requires Vac14p, an activator of the lipid kinase Fab1p. J Cell Biol. 2002;156:1015-28 pubmed
    ..These findings demonstrate that Vac14p regulates the levels of PtdIns(3,5)P(2) and provide insight into why PtdIns(3,5)P(2) levels rise in response to osmotic stress. ..
  5. Dove S, McEwen R, Mayes A, Hughes D, Beggs J, Michell R. Vac14 controls PtdIns(3,5)P(2) synthesis and Fab1-dependent protein trafficking to the multivesicular body. Curr Biol. 2002;12:885-93 pubmed
    The PtdIns3P 5-kinase Fab1 makes PtdIns(3,5)P(2), a phosphoinositide essential for retrograde trafficking between the vacuole/lysosome and the late endosome and also for trafficking of some proteins into the vacuole via multivesicular ..
  6. Gary J, Wurmser A, Bonangelino C, Weisman L, Emr S. Fab1p is essential for PtdIns(3)P 5-kinase activity and the maintenance of vacuolar size and membrane homeostasis. J Cell Biol. 1998;143:65-79 pubmed
    The Saccharomyces cerevisiae FAB1 gene encodes a 257-kD protein that contains a cysteine-rich RING-FYVE domain at its NH2-terminus and a kinase domain at its COOH terminus...
  7. Jin N, Chow C, Liu L, Zolov S, Bronson R, Davisson M, et al. VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse. EMBO J. 2008;27:3221-34 pubmed publisher
    ..a scaffold for the PI(3,5)P(2) regulatory complex by direct contact with the known regulators of PI(3,5)P(2): Fig4, Fab1, Vac7 and Atg18...
  8. Botelho R, Efe J, Teis D, Emr S. Assembly of a Fab1 phosphoinositide kinase signaling complex requires the Fig4 phosphoinositide phosphatase. Mol Biol Cell. 2008;19:4273-86 pubmed publisher
    ..The lipid kinase Fab1 converts phosphatidylinositol-3-phosphate [PtdIns(3)P] to PtdIns(3,5)P(2)...
  9. Gary J, Sato T, Stefan C, Bonangelino C, Weisman L, Emr S. Regulation of Fab1 phosphatidylinositol 3-phosphate 5-kinase pathway by Vac7 protein and Fig4, a polyphosphoinositide phosphatase family member. Mol Biol Cell. 2002;13:1238-51 pubmed
    The Saccharomyces cerevisiae FAB1 gene encodes the sole phosphatidylinositol 3-phosphate [PtdIns(3)P] 5-kinase responsible for synthesis of the polyphosphoinositide PtdIns(3,5)P(2)...

More Information

Publications23

  1. Bidlingmaier S, Snyder M. Large-scale identification of genes important for apical growth in Saccharomyces cerevisiae by directed allele replacement technology (DART) screening. Funct Integr Genomics. 2002;1:345-56 pubmed
    ..29 insertion alleles, containing mutations in 17 different genes ( SMY1, SPA2, PAN1, SLA1, SLA2, CBK1, SEC22, FAB1, VPS36, VID22, RAS2, ECM33, OPI3, API1/YDR372c, API2/YDR525w, API3/YKR020w, and API4/YNL051w), which alter the ..
  2. Paumi C, Menendez J, Arnoldo A, Engels K, Iyer K, Thaminy S, et al. Mapping protein-protein interactions for the yeast ABC transporter Ycf1p by integrated split-ubiquitin membrane yeast two-hybrid analysis. Mol Cell. 2007;26:15-25 pubmed
    ..Our study provides proof of principle that iMYTH is an ideal methodology to identify physiological interactors and regulators of ABC transporters and other yeast transmembrane proteins...
  3. Georgakopoulos T, Koutroubas G, Vakonakis I, Tzermia M, Prokova V, Voutsina A, et al. Functional analysis of the Saccharomyces cerevisiae YFR021w/YGR223c/YPL100w ORF family suggests relations to mitochondrial/peroxisomal functions and amino acid signalling pathways. Yeast. 2001;18:1155-71 pubmed
    ..We found no unique phenotype attributed to the deletion of YGR223c. However, its function interferes with the function of the other two ORFs, as revealed by the effects of double- and triple-ORF deletions. ..
  4. Thumm M, Busse R, Scacioc A, Stephan M, Janshoff A, Kühnel K, et al. It takes two to tango: PROPPINs use two phosphoinositide-binding sites. Autophagy. 2013;9:106-7 pubmed publisher
    ..We expect that membrane association of PROPPINs is further stabilized by membrane insertions and interactions with other proteins. ..
  5. Jin N, Mao K, Jin Y, Tevzadze G, Kauffman E, Park S, et al. Roles for PI(3,5)P2 in nutrient sensing through TORC1. Mol Biol Cell. 2014;25:1171-85 pubmed publisher
    ..These findings reveal PI(3,5)P(2) as a general regulator of TORC1 and suggest that PI(3,5)P(2) provides a platform for TORC1 signaling from lysosomes. ..
  6. Yadav P, Rajasekharan R. The m6A methyltransferase Ime4 epitranscriptionally regulates triacylglycerol metabolism and vacuolar morphology in haploid yeast cells. J Biol Chem. 2017;292:13727-13744 pubmed publisher
    ..Because the yeast vacuole is an analogue of the mammalian lysosome, our findings pave the way to better understand the role of m6A methylation in lysosome-related functions and diseases. ..
  7. Nomura W, Maeta K, Inoue Y. Phosphatidylinositol 3,5-bisphosphate is involved in methylglyoxal-induced activation of the Mpk1 mitogen-activated protein kinase cascade in Saccharomyces cerevisiae. J Biol Chem. 2017;292:15039-15048 pubmed publisher
    ..Our results suggest that PtdIns(3,5)P2 is specifically involved in the MG-induced activation of the Mpk1 MAPK cascade and in the cellular adaptation to MG-induced stress. ..
  8. Tahirovic S, Schorr M, Mayinger P. Regulation of intracellular phosphatidylinositol-4-phosphate by the Sac1 lipid phosphatase. Traffic. 2005;6:116-30 pubmed
    ..We conclude that Sac1p functions in confining PtdIns(4)P-dependent processes to specific intracellular membranes. ..
  9. Alghamdi T, Ho C, Mrakovic A, Taylor D, Mao D, Botelho R. Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and function. J Biol Chem. 2013;288:9363-72 pubmed publisher
    ..The Fab1/PIKfyve lipid kinase phosphorylates PtdIns(3)P into PtdIns(3,5)P2 whereas the Fig4/Sac3 lipid phosphatase ..
  10. Wei W, Smith N, Wu X, Kim H, Seravalli J, Khalimonchuk O, et al. YCF1-mediated cadmium resistance in yeast is dependent on copper metabolism and antioxidant enzymes. Antioxid Redox Signal. 2014;21:1475-89 pubmed publisher
  11. Ho C, Choy C, Wattson C, Johnson D, Botelho R. The Fab1/PIKfyve phosphoinositide phosphate kinase is not necessary to maintain the pH of lysosomes and of the yeast vacuole. J Biol Chem. 2015;290:9919-28 pubmed publisher
    ..For example, quinacrine, an acidotropic fluorescent base, does not accumulate in the vacuoles of fab1Δ yeast...
  12. López Martínez G, Margalef Català M, Salinas F, Liti G, Cordero Otero R. ATG18 and FAB1 are involved in dehydration stress tolerance in Saccharomyces cerevisiae. PLoS ONE. 2015;10:e0119606 pubmed publisher
    ..We report new phenotypes for these genes using a previously validated test. Finally, our data illustrates the power of this approach in the investigation of the complex cell dehydration phenotype. ..
  13. Jin N, Jin Y, Weisman L. Early protection to stress mediated by CDK-dependent PI3,5P2 signaling from the vacuole/lysosome. J Cell Biol. 2017;216:2075-2090 pubmed publisher
    ..lipid PI3,5P2 We show that Pho85/CDK5 directly phosphorylates and positively regulates the PI3P-5 kinase Fab1/PIKfyve complex and provide evidence that this regulation is conserved in mammalian cells...
  14. Phelan J, Millson S, Parker P, Piper P, Cooke F. Fab1p and AP-1 are required for trafficking of endogenously ubiquitylated cargoes to the vacuole lumen in S. cerevisiae. J Cell Sci. 2006;119:4225-34 pubmed
    ..Our data imply that AP-1 is required for some Fab1p and PtdIns(3,5)P2-dependent processes. ..