Gene Symbol: FAA1
Description: long-chain fatty acid-CoA ligase FAA1
Alias: long-chain fatty acid-CoA ligase FAA1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Yadav P, Rajasekharan R. The m6A methyltransferase Ime4 epitranscriptionally regulates triacylglycerol metabolism and vacuolar morphology in haploid yeast cells. J Biol Chem. 2017;292:13727-13744 pubmed publisher
    ..regulates triacylglycerol metabolism and vacuolar morphology through the long-chain fatty acyl-CoA synthetase Faa1, independently of the RNA methylation complex (MIS complex)...
  2. Johnson D, Knoll L, Rowley N, Gordon J. Genetic analysis of the role of Saccharomyces cerevisiae acyl-CoA synthetase genes in regulating protein N-myristoylation. J Biol Chem. 1994;269:18037-46 pubmed
    ..We have isolated and characterized three unlinked Fatty Acid Activation genes from S. cerevisiae, FAA1, FAA2, and FAA3...
  3. Currie E, Guo X, Christiano R, Chitraju C, Kory N, Harrison K, et al. High confidence proteomic analysis of yeast LDs identifies additional droplet proteins and reveals connections to dolichol synthesis and sterol acetylation. J Lipid Res. 2014;55:1465-77 pubmed publisher
    ..Our results provide a highly specific list of yeast LD proteins and reveal that the vast majority of these proteins are involved in lipid metabolism. ..
  4. Li X, Guo D, Cheng Y, Zhu F, Deng Z, Liu T. Overproduction of fatty acids in engineered Saccharomyces cerevisiae. Biotechnol Bioeng. 2014;111:1841-52 pubmed publisher
    ..All these efforts, particularly the discovery of the limiting step are critical for developing a "cell factory" for the overproduction of fatty acids by using type I fatty acids synthase in yeast or other fungi. ..
  5. Knoll L, Johnson D, Gordon J. Biochemical studies of three Saccharomyces cerevisiae acyl-CoA synthetases, Faa1p, Faa2p, and Faa3p. J Biol Chem. 1994;269:16348-56 pubmed
    ..coli during exponential growth at 24 or 37 degrees C and (ii) measurement of the incorporation of [3H]myristate into a yeast N-myristoylprotein coexpressed with Nmt1p and Faa1p, Faa2p, or Faa3p in the fadD- strain. ..
  6. Nozawa M, Takahashi T, Hara S, Mizoguchi H. A role of Saccharomyces cerevisiae fatty acid activation protein 4 in palmitoyl-CoA pool for growth in the presence of ethanol. J Biosci Bioeng. 2002;93:288-95 pubmed
    ..the growth defect caused by snc1 overexpression was rescued by the overexpression of FAA4, but not of FAA1, which plays a predominant role in laboratory strains...
  7. Tong F, Black P, Bivins L, Quackenbush S, Ctrnacta V, DiRusso C. Direct interaction of Saccharomyces cerevisiae Faa1p with the Omi/HtrA protease orthologue Ynm3p alters lipid homeostasis. Mol Genet Genomics. 2006;275:330-43 pubmed
    ..Additional strain-specific phenotypes associated with deletion of YNM3 included inability to grow on non-fermentable carbon sources and altered cellular morphology. ..
  8. Li H, Melton E, Quackenbush S, DiRusso C, Black P. Mechanistic studies of the long chain acyl-CoA synthetase Faa1p from Saccharomyces cerevisiae. Biochim Biophys Acta. 2007;1771:1246-53 pubmed
    ..In the yeast Saccharomyces cerevisiae the principal ACSL is Faa1p (encoded by the FAA1 gene). The preferred substrates for this enzyme are cis-monounsaturated long chain fatty acids...
  9. Chen Y, Li F, Guo J, Liu G, Guo X, Xiao D. Enhanced ethyl caproate production of Chinese liquor yeast by overexpressing EHT1 with deleted FAA1. J Ind Microbiol Biotechnol. 2014;41:563-72 pubmed publisher
    ..we constructed a recombinant strain EY15 by overexpressing EHT1 (encoding ethanol hexanoyl transferase), in which FAA1 (encoding acyl-CoA synthetases) was deleted...

More Information


  1. Knoll L, Johnson D, Gordon J. Complementation of Saccharomyces cerevisiae strains containing fatty acid activation gene (FAA) deletions with a mammalian acyl-CoA synthetase. J Biol Chem. 1995;270:10861-7 pubmed
    ..The functionally interchangeable FAA1 and FAA4 genes are responsible for activation of these imported fatty acids...
  2. Athenstaedt K, Zweytick D, Jandrositz A, Kohlwein S, Daum G. Identification and characterization of major lipid particle proteins of the yeast Saccharomyces cerevisiae. J Bacteriol. 1999;181:6441-8 pubmed
    ..Strains with YBR177c, YMR313c, and YKL140w deleted exhibited phospholipid and/or neutral lipid patterns that were different from the wild-type strain and thus may be further candidate ORFs involved in yeast lipid metabolism. ..
  3. Faergeman N, Black P, Zhao X, Knudsen J, DiRusso C. The Acyl-CoA synthetases encoded within FAA1 and FAA4 in Saccharomyces cerevisiae function as components of the fatty acid transport system linking import, activation, and intracellular Utilization. J Biol Chem. 2001;276:37051-9 pubmed
    ..acid, long-chain fatty acid transport was shown to be severely restricted in a faa1 Delta faa4 Delta strain...
  4. Nakahara K, Ohkuni A, Kitamura T, Abe K, Naganuma T, Ohno Y, et al. The Sjögren-Larsson syndrome gene encodes a hexadecenal dehydrogenase of the sphingosine 1-phosphate degradation pathway. Mol Cell. 2012;46:461-71 pubmed publisher
    ..Moreover, we demonstrate that yeast Faa1 and Faa4 and mammalian ACSL family members are acyl-CoA synthetases involved in the sphingolipid-to-glycerolipid ..
  5. Choi J, Martin C. The Saccharomyces cerevisiae FAT1 gene encodes an acyl-CoA synthetase that is required for maintenance of very long chain fatty acid levels. J Biol Chem. 1999;274:4671-83 pubmed
    ..Simultaneous disruption of FAA1 and FAA4, which encode long chain (C14-C18) fatty acyl-CoA synthetases, effectively blocks the import of long chain ..
  6. Chen L, Zhang J, Lee J, Chen W. Enhancement of free fatty acid production in Saccharomyces cerevisiae by control of fatty acyl-CoA metabolism. Appl Microbiol Biotechnol. 2014;98:6739-50 pubmed publisher
    ..For this purpose, firstly, haploid S. cerevisiae double deletion strain ?faa1?faa4 was constructed, in which the genes FAA1 and FAA4 encoding two acyl-CoA synthetases were deleted...
  7. Narita T, Naganuma T, Sase Y, Kihara A. Long-chain bases of sphingolipids are transported into cells via the acyl-CoA synthetases. Sci Rep. 2016;6:25469 pubmed publisher
    ..Here, we found that the yeast acyl-CoA synthetases (ACSs) Faa1 and Faa4 are redundantly involved in LCB uptake...
  8. Darwiche R, Mène Saffrané L, Gfeller D, Asojo O, Schneiter R. The pathogen-related yeast protein Pry1, a member of the CAP protein superfamily, is a fatty acid-binding protein. J Biol Chem. 2017;292:8304-8314 pubmed publisher
    ..i>in vitro, and its function was essential for fatty acid export in cells lacking the acyl-CoA synthetases Faa1 and Faa4...
  9. Zou Z, Tong F, Faergeman N, Børsting C, Black P, DiRusso C. Vectorial acylation in Saccharomyces cerevisiae. Fat1p and fatty acyl-CoA synthetase are interacting components of a fatty acid import complex. J Biol Chem. 2003;278:16414-22 pubmed
    ..Multicopy extragenic suppressors were selected in strains carrying deletions in FAA1 and FAA4 or FAA1 and FAT1...
  10. Scharnewski M, Pongdontri P, Mora G, Hoppert M, Fulda M. Mutants of Saccharomyces cerevisiae deficient in acyl-CoA synthetases secrete fatty acids due to interrupted fatty acid recycling. FEBS J. 2008;275:2765-78 pubmed publisher
    ..Therefore, we propose the recycling of endogenous fatty acids generated in the course of lipid remodelling as a major task of both acyl-CoA synthetases Faa1p and Faa4p. ..
  11. Jacquier N, Schneiter R. Ypk1, the yeast orthologue of the human serum- and glucocorticoid-induced kinase, is required for efficient uptake of fatty acids. J Cell Sci. 2010;123:2218-27 pubmed publisher
    ..Consistent with such a proposition, mutations that block early steps of endocytosis display reduced levels of fatty-acid uptake. ..