EXO1

Summary

Gene Symbol: EXO1
Description: Rad2 family nuclease EXO1
Alias: DHS1, Rad2 family nuclease EXO1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Nakada D, Hirano Y, Sugimoto K. Requirement of the Mre11 complex and exonuclease 1 for activation of the Mec1 signaling pathway. Mol Cell Biol. 2004;24:10016-25 pubmed
    ..We show here that the Mre11 complex functions together with exonuclease 1 (Exo1) in activation of the Mec1 signaling pathway after DNA damage and replication block...
  2. Segurado M, Diffley J. Separate roles for the DNA damage checkpoint protein kinases in stabilizing DNA replication forks. Genes Dev. 2008;22:1816-27 pubmed publisher
    ..that the sensitivity of rad53 mutants to DNA-damaging agents can be almost completely suppressed by deletion of the EXO1 gene, which encodes an enigmatic flap endonuclease...
  3. Giannattasio M, Follonier C, Tourrière H, Puddu F, Lazzaro F, Pasero P, et al. Exo1 competes with repair synthesis, converts NER intermediates to long ssDNA gaps, and promotes checkpoint activation. Mol Cell. 2010;40:50-62 pubmed publisher
    ..We show that during repair of UV lesions in noncycling cells, Exo1-mediated processing of nucleotide excision repair (NER) intermediates competes with repair DNA synthesis...
  4. Bertuch A, Lundblad V. EXO1 contributes to telomere maintenance in both telomerase-proficient and telomerase-deficient Saccharomyces cerevisiae. Genetics. 2004;166:1651-9 pubmed
    Previous work in budding yeast has indicated that telomeres are protected, at least in part, from the action of Exo1, which degrades the C-rich strand of partially uncapped telomeres...
  5. Anbalagan S, Bonetti D, Lucchini G, Longhese M. Rif1 supports the function of the CST complex in yeast telomere capping. PLoS Genet. 2011;7:e1002024 pubmed publisher
    ..DNA damage checkpoint activation and lethality in cdc13 rif1? cells are partially counteracted by the lack of the Exo1 nuclease, which is involved in telomeric single-stranded DNA generation...
  6. Tishkoff D, Boerger A, Bertrand P, Filosi N, Gaida G, Kane M, et al. Identification and characterization of Saccharomyces cerevisiae EXO1, a gene encoding an exonuclease that interacts with MSH2. Proc Natl Acad Sci U S A. 1997;94:7487-92 pubmed
    ..One gene was found to encode a homologue of Schizosaccharomyces pombe EXO1, a double-stranded DNA-specific 5'-3' exonuclease. S. cerevisiae EXO1 interacted with both S...
  7. Tsolou A, Lydall D. Mrc1 protects uncapped budding yeast telomeres from exonuclease EXO1. DNA Repair (Amst). 2007;6:1607-17 pubmed
    ..Consistent with this, deletion of EXO1, encoding a nuclease that contributes to single stranded DNA accumulation after telomere uncapping, improves growth ..
  8. Smith S, Gupta A, Kolodner R, Myung K. Suppression of gross chromosomal rearrangements by the multiple functions of the Mre11-Rad50-Xrs2 complex in Saccharomyces cerevisiae. DNA Repair (Amst). 2005;4:606-17 pubmed
    ..However, the non-homologous end joining function of MRX complex does not appear to participate in the suppression of GCRs. ..
  9. Barber L, Ward T, Hartley J, McHugh P. DNA interstrand cross-link repair in the Saccharomyces cerevisiae cell cycle: overlapping roles for PSO2 (SNM1) with MutS factors and EXO1 during S phase. Mol Cell Biol. 2005;25:2297-309 pubmed
    ..Here we establish a novel overlapping function for PSO2 with MutS mismatch repair factors and the 5'-3' exonuclease Exo1 in the repair of DNA ICLs, which is confined to S phase...

More Information

Publications69

  1. Gueneau E, Dherin C, Legrand P, Tellier Lebegue C, Gilquin B, Bonnesoeur P, et al. Structure of the MutL? C-terminal domain reveals how Mlh1 contributes to Pms1 endonuclease site. Nat Struct Mol Biol. 2013;20:461-8 pubmed publisher
    ..The structures of the ternary complexes between MutL?(CTD) and Exo1 or Ntg2 fragments reveal the binding mode of the MIP-box motif shared by several Mlh1 partners...
  2. Kaochar S, Shanks L, Weinert T. Checkpoint genes and Exo1 regulate nearby inverted repeat fusions that form dicentric chromosomes in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2010;107:21605-10 pubmed publisher
    ..inverted repeat fusion, and that their activities are distinguishable by their interactions with exonuclease 1 (Exo1)...
  3. Maringele L, Lydall D. EXO1-dependent single-stranded DNA at telomeres activates subsets of DNA damage and spindle checkpoint pathways in budding yeast yku70Delta mutants. Genes Dev. 2002;16:1919-33 pubmed
    ..increasing levels of single-stranded DNA in subtelomeric Y' regions, and find that the mismatch repair-associated EXO1 gene is required for both ssDNA generation and cell cycle arrest of yku70Delta mutants...
  4. Foster S, Balestrini A, Petrini J. Functional interplay of the Mre11 nuclease and Ku in the response to replication-associated DNA damage. Mol Cell Biol. 2011;31:4379-89 pubmed publisher
    ..CPT) and methyl methanesulfonate (MMS) sensitivity of nuclease-deficient mre11-3 and sae2? mutants in an Exo1-dependent manner...
  5. Hodgson A, Terentyev Y, Johnson R, Bishop Bailey A, Angevin T, Croucher A, et al. Mre11 and Exo1 contribute to the initiation and processivity of resection at meiotic double-strand breaks made independently of Spo11. DNA Repair (Amst). 2011;10:138-48 pubmed publisher
    ..Analysis of strains with different mutant alleles of MRE11 (mre11-58S and mre11-H125N) and deleted for EXO1 indicated that these two nucleases make significant contributions to repair of VDE-DSBs...
  6. Moreau S, Morgan E, Symington L. Overlapping functions of the Saccharomyces cerevisiae Mre11, Exo1 and Rad27 nucleases in DNA metabolism. Genetics. 2001;159:1423-33 pubmed
    ..Mutation of EXO1, which encodes a 5' to 3' exonuclease, was found to increase the ionizing radiation sensitivity of both mre11Delta ..
  7. Tran P, Simon J, Liskay R. Interactions of Exo1p with components of MutLalpha in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2001;98:9760-5 pubmed
    ..Finally, our results show that much of the increase in spontaneous mutation observed in an exo1Delta strain is REV3-dependent, in turn suggesting that Exo1p is also involved in one or more MMR-independent mutation avoidance pathways. ..
  8. Chen H, Lisby M, Symington L. RPA coordinates DNA end resection and prevents formation of DNA hairpins. Mol Cell. 2013;50:589-600 pubmed publisher
    ..We found that RPA depletion eliminated both the Sgs1-Dna2- and Exo1-dependent extensive resection pathways and synergized with mre11? to prevent end resection...
  9. Eapen V, Sugawara N, Tsabar M, Wu W, Haber J. The Saccharomyces cerevisiae chromatin remodeler Fun30 regulates DNA end resection and checkpoint deactivation. Mol Cell Biol. 2012;32:4727-40 pubmed publisher
    ..Fun30 is recruited to an HO endonuclease-induced DSB and acts in both the Exo1-dependent and Sgs1-dependent resection pathways...
  10. Karras G, Fumasoni M, Sienski G, Vanoli F, Branzei D, Jentsch S. Noncanonical role of the 9-1-1 clamp in the error-free DNA damage tolerance pathway. Mol Cell. 2013;49:536-46 pubmed publisher
    ..that the PCNA-related 9-1-1 complex, which is typically linked to checkpoint signaling, participates together with Exo1 nuclease in error-free DDT...
  11. Lazzaro F, Sapountzi V, Granata M, Pellicioli A, Vaze M, Haber J, et al. Histone methyltransferase Dot1 and Rad9 inhibit single-stranded DNA accumulation at DSBs and uncapped telomeres. EMBO J. 2008;27:1502-12 pubmed publisher
  12. Keelagher R, Cotton V, Goldman A, Borts R. Separable roles for Exonuclease I in meiotic DNA double-strand break repair. DNA Repair (Amst). 2011;10:126-37 pubmed publisher
    b>Exo1 is a member of the Rad2 protein family and possesses both 5'-3' exonuclease and 5' flap endonuclease activities...
  13. Hombauer H, Campbell C, Smith C, Desai A, Kolodner R. Visualization of eukaryotic DNA mismatch repair reveals distinct recognition and repair intermediates. Cell. 2011;147:1040-53 pubmed publisher
    ..this localized pool accounted for 10%-15% of MMR in wild-type cells but was essential for MMR in the absence of Exo1. Unexpectedly, Mlh1-Pms1 formed nuclear foci that, although dependent on Msh2-Msh6 for formation, rarely ..
  14. Marrero V, Symington L. Extensive DNA end processing by exo1 and sgs1 inhibits break-induced replication. PLoS Genet. 2010;6:e1001007 pubmed publisher
    ..The rare chromosome rearrangements due to BIR template switching at repeated sequences were increased in the exo1Delta sgs1Delta mutant, suggesting that reduced resection can decrease the fidelity of homologous recombination. ..
  15. Lydeard J, Lipkin Moore Z, Jain S, Eapen V, Haber J. Sgs1 and exo1 redundantly inhibit break-induced replication and de novo telomere addition at broken chromosome ends. PLoS Genet. 2010;6:e1000973 pubmed publisher
    ..Resection proceeds by two pathways dependent on Exo1 or the BLM homolog, Sgs1...
  16. Costelloe T, Louge R, Tomimatsu N, Mukherjee B, Martini E, Khadaroo B, et al. The yeast Fun30 and human SMARCAD1 chromatin remodellers promote DNA end resection. Nature. 2012;489:581-4 pubmed publisher
    ..known as RBBP8 or CTIP in humans) initiate end resection, whereas long-range resection depends on the exonuclease Exo1, or the helicase-topoisomerase complex Sgs1-Top3-Rmi1 together with the endonuclease Dna2 (refs 1-6)...
  17. Morin I, Ngo H, Greenall A, Zubko M, Morrice N, Lydall D. Checkpoint-dependent phosphorylation of Exo1 modulates the DNA damage response. EMBO J. 2008;27:2400-10 pubmed publisher
    b>Exo1 is a nuclease involved in mismatch repair, DSB repair, stalled replication fork processing and in the DNA damage response triggered by dysfunctional telomeres...
  18. Mimitou E, Symington L. Sae2, Exo1 and Sgs1 collaborate in DNA double-strand break processing. Nature. 2008;455:770-4 pubmed publisher
    ..but several factors have been implicated, including the Mre11 complex (Mre11-Rad50-Xrs2/NBS1), Sae2/CtIP/Ctp1 and Exo1. Here we demonstrate that yeast Exo1 nuclease and Sgs1 helicase function in alternative pathways for DSB processing...
  19. Deng S, Gibb B, de Almeida M, Greene E, Symington L. RPA antagonizes microhomology-mediated repair of DNA double-strand breaks. Nat Struct Mol Biol. 2014;21:405-12 pubmed publisher
    ..We propose that the helix-destabilizing activity of RPA channels ssDNA intermediates from mutagenic MMEJ to error-free homologous recombination, thus preserving genome integrity. ..
  20. Maringele L, Lydall D. EXO1 plays a role in generating type I and type II survivors in budding yeast. Genetics. 2004;166:1641-9 pubmed
    ..Here we demonstrate that the Exo1 exonuclease is an initiator of the recombination process that allows cells to escape senescence and become immortal ..
  21. Cotta Ramusino C, Fachinetti D, Lucca C, Doksani Y, Lopes M, Sogo J, et al. Exo1 processes stalled replication forks and counteracts fork reversal in checkpoint-defective cells. Mol Cell. 2005;17:153-9 pubmed
    ..technique (2D gel) and psoralen crosslinking combined with electron microscopy (EM), we found that the Exo1 exonuclease is recruited to stalled forks and, in rad53 mutants, counteracts reversed fork accumulation by ..
  22. Huang D, Piening B, Paulovich A. The preference for error-free or error-prone postreplication repair in Saccharomyces cerevisiae exposed to low-dose methyl methanesulfonate is cell cycle dependent. Mol Cell Biol. 2013;33:1515-27 pubmed publisher
    ..However, when PRR is restricted to the G2 phase, cells utilize REV3-dependent translesion synthesis, which requires a MEC1-dependent delay and results in significant hypermutability. ..
  23. Bonetti D, Clerici M, Manfrini N, Lucchini G, Longhese M. The MRX complex plays multiple functions in resection of Yku- and Rif2-protected DNA ends. PLoS ONE. 2010;5:e14142 pubmed publisher
    ..DNA ends, which is specifically inhibited during the G1 cell cycle phase, requires the MRX complex, Sae2, Sgs1 and Exo1. Moreover, it is negatively regulated by the non-homologous end-joining component Yku and the telomeric protein ..
  24. Vodenicharov M, Laterreur N, Wellinger R. Telomere capping in non-dividing yeast cells requires Yku and Rap1. EMBO J. 2010;29:3007-19 pubmed publisher
    ..The results indeed show that both Exo1 and the Mre11/Rad50/Xrs2 complex are required for telomeric resection after Yku loss in non-dividing cells...
  25. Zhu Z, Chung W, Shim E, Lee S, Ira G. Sgs1 helicase and two nucleases Dna2 and Exo1 resect DNA double-strand break ends. Cell. 2008;134:981-94 pubmed publisher
    ..annealing between distant repeats while the remaining long-range resection activity depends on the exonuclease Exo1. In exo1Deltasgs1Delta double mutants, the MRX complex together with Sae2 nuclease generate, in a stepwise manner, ..
  26. Gravel S, Chapman J, Magill C, Jackson S. DNA helicases Sgs1 and BLM promote DNA double-strand break resection. Genes Dev. 2008;22:2767-72 pubmed publisher
    ..Here, we reveal that in the absence of exonuclease Exo1 activity, deletion or mutation of the Saccharomyces cerevisiae RecQ-family helicase, Sgs1, causes pronounced ..
  27. Zakharyevich K, Ma Y, Tang S, Hwang P, Boiteux S, Hunter N. Temporally and biochemically distinct activities of Exo1 during meiosis: double-strand break resection and resolution of double Holliday junctions. Mol Cell. 2010;40:1001-15 pubmed publisher
    The Rad2/XPG family nuclease, Exo1, functions in a variety of DNA repair pathways. During meiosis, Exo1 promotes crossover recombination and thereby facilitates chromosome segregation at the first division...
  28. Xu L, Petreaca R, Gasparyan H, Vu S, Nugent C. TEN1 is essential for CDC13-mediated telomere capping. Genetics. 2009;183:793-810 pubmed publisher
    ..Cdk1 activity is required to generate these single-stranded regions, and deleting the EXO1 nuclease partially suppresses ten1-ts growth defects...
  29. Liberti S, LARREA A, Kunkel T. Exonuclease 1 preferentially repairs mismatches generated by DNA polymerase α. DNA Repair (Amst). 2013;12:92-6 pubmed publisher
    The Saccharomyces cerevisiae EXO1 gene encodes a 5' exonuclease that participates in mismatch repair (MMR) of DNA replication errors...
  30. Nicholson A, Hendrix M, Jinks Robertson S, Crouse G. Regulation of mitotic homeologous recombination in yeast. Functions of mismatch repair and nucleotide excision repair genes. Genetics. 2000;154:133-46 pubmed
    ..In addition to the yeast MutS and MutL homologs, the exonuclease Exo1p and the nucleotide excision repair proteins Rad1p and Rad10p were found to have roles in inhibiting recombination between mismatched substrates. ..
  31. Symington L, Kang L, Moreau S. Alteration of gene conversion tract length and associated crossing over during plasmid gap repair in nuclease-deficient strains of Saccharomyces cerevisiae. Nucleic Acids Res. 2000;28:4649-56 pubmed
    A plasmid gap repair assay was used to assess the role of three known nucleases, Exo1, Mre11 and Rad1, in the processing of DNA ends and resolution of recombination intermediates during double-strand gap repair...
  32. Chen X, Niu H, Chung W, Zhu Z, Papusha A, Shim E, et al. Cell cycle regulation of DNA double-strand break end resection by Cdk1-dependent Dna2 phosphorylation. Nat Struct Mol Biol. 2011;18:1015-9 pubmed publisher
    ..The Mre11-Rad50-Xrs2 complex with Sae2 initiates resection, whereas two nucleases, Exo1 and Dna2, and the DNA helicase-topoisomerase complex Sgs1-Top3-Rmi1 generate longer ssDNA at DSBs...
  33. Tsubouchi H, Ogawa H. Exo1 roles for repair of DNA double-strand breaks and meiotic crossing over in Saccharomyces cerevisiae. Mol Biol Cell. 2000;11:2221-33 pubmed
    ..Multiple copies of the EXO1 gene, encoding a 5' to 3' double-strand DNA exonuclease, were found to suppress the high MMS sensitivity of these ..
  34. Tran P, Erdeniz N, Dudley S, Liskay R. Characterization of nuclease-dependent functions of Exo1p in Saccharomyces cerevisiae. DNA Repair (Amst). 2002;1:895-912 pubmed
    ..Examining the genetic interaction of these two exo1 mutations with rad27Delta suggest that the Exo1p flap-endonuclease activity and not the dsDNA 5'-3' exonuclease is ..
  35. Lee S, Bressan D, Petrini J, Haber J. Complementation between N-terminal Saccharomyces cerevisiae mre11 alleles in DNA repair and telomere length maintenance. DNA Repair (Amst). 2002;1:27-40 pubmed
    ..We propose that at least two separate activities associated with the N-terminus of Mre11p are required for its mitotic function. ..
  36. Mantiero D, Clerici M, Lucchini G, Longhese M. Dual role for Saccharomyces cerevisiae Tel1 in the checkpoint response to double-strand breaks. EMBO Rep. 2007;8:380-7 pubmed
    ..DNA accumulation at the ends of DSBs, and this checkpoint function can be overcome by overproducing the exonuclease Exo1. Second, Tel1 can activate the checkpoint response to DSBs independently of Mec1, although its signalling activity ..
  37. Mimitou E, Symington L. Ku prevents Exo1 and Sgs1-dependent resection of DNA ends in the absence of a functional MRX complex or Sae2. EMBO J. 2010;29:3358-69 pubmed publisher
    ..the absence of end protection by Ku, the requirement for the MRX complex is bypassed and resection is executed by Exo1. In contrast, both the Exo1 and Sgs1 resection pathways contribute to DSB processing in the absence of Ku and Sae2 ..
  38. Zubko M, Guillard S, Lydall D. Exo1 and Rad24 differentially regulate generation of ssDNA at telomeres of Saccharomyces cerevisiae cdc13-1 mutants. Genetics. 2004;168:103-15 pubmed
    ..We show that Exo1 is unique among the repair genes tested because like Rad9 and Rad24 checkpoint proteins, Exo1 inhibits the growth ..
  39. Bonetti D, Clerici M, Anbalagan S, Martina M, Lucchini G, Longhese M. Shelterin-like proteins and Yku inhibit nucleolytic processing of Saccharomyces cerevisiae telomeres. PLoS Genet. 2010;6:e1000966 pubmed publisher
    ..Rap1 and Rif2 prevent telomere degradation by inhibiting MRX access to telomeres, which are also protected from the Exo1 nuclease by Yku...
  40. Chen X, Cui D, Papusha A, Zhang X, Chu C, Tang J, et al. The Fun30 nucleosome remodeller promotes resection of DNA double-strand break ends. Nature. 2012;489:576-80 pubmed publisher
    ..Two redundant pathways of extensive resection have been described both in cells and in vitro, one relying on Exo1 exonuclease and the other on Sgs1 helicase and Dna2 nuclease...
  41. Bonetti D, Martina M, Clerici M, Lucchini G, Longhese M. Multiple pathways regulate 3' overhang generation at S. cerevisiae telomeres. Mol Cell. 2009;35:70-81 pubmed publisher
    ..No processing activity is detectable in sae2Delta sgs1Delta cells, while the Exo1 exonuclease contributes to telomere end processing and elongation in both sae2Delta and sgs1Delta cells, suggesting ..
  42. Amin N, Nguyen M, Oh S, Kolodner R. exo1-Dependent mutator mutations: model system for studying functional interactions in mismatch repair. Mol Cell Biol. 2001;21:5142-55 pubmed
    b>EXO1 interacts with MSH2 and MLH1 and has been proposed to be a redundant exonuclease that functions in mismatch repair (MMR)...
  43. Lewis L, Karthikeyan G, Westmoreland J, Resnick M. Differential suppression of DNA repair deficiencies of Yeast rad50, mre11 and xrs2 mutants by EXO1 and TLC1 (the RNA component of telomerase). Genetics. 2002;160:49-62 pubmed
    ..expressed cDNAs that suppress the DNA repair deficiency of rad50 mutants yielded multiple isolates of two genes: EXO1 and TLC1...
  44. Ngo H, Lydall D. Survival and growth of yeast without telomere capping by Cdc13 in the absence of Sgs1, Exo1, and Rad9. PLoS Genet. 2010;6:e1001072 pubmed publisher
    ..We found that simultaneous inactivation of Sgs1, Exo1, and Rad9, three DNA damage response (DDR) proteins, is sufficient to allow cell division in the absence of Cdc13...
  45. Shim E, Chung W, Nicolette M, Zhang Y, Davis M, Zhu Z, et al. Saccharomyces cerevisiae Mre11/Rad50/Xrs2 and Ku proteins regulate association of Exo1 and Dna2 with DNA breaks. EMBO J. 2010;29:3370-80 pubmed publisher
    ..MRX) and Sae2 are implicated in the onset of 5'-strand resection, whereas Sgs1/Top3/Rmi1 with Dna2 and Exo1 are involved in extensive resection...
  46. Li L, Murphy K, Kanevets U, Reha Krantz L. Sensitivity to phosphonoacetic acid: a new phenotype to probe DNA polymerase delta in Saccharomyces cerevisiae. Genetics. 2005;170:569-80 pubmed
    ..proteins-Msh2, Mlh1, or Pms1-and severe sensitivity to PAA is observed in the absence of the core proteins Msh6 or Exo1, but not Msh3...
  47. Potenski C, Niu H, Sung P, Klein H. Avoidance of ribonucleotide-induced mutations by RNase H2 and Srs2-Exo1 mechanisms. Nature. 2014;511:251-4 pubmed publisher
    ..In addition, Srs2 interacts with and enhances the activity of the nuclease Exo1, to generate a DNA gap in preparation for repair...
  48. Balogun F, Truman A, Kron S. DNA resection proteins Sgs1 and Exo1 are required for G1 checkpoint activation in budding yeast. DNA Repair (Amst). 2013;12:751-60 pubmed publisher
    ..Here we demonstrate for the first time that the resection proteins Sgs1 and Exo1 are required for efficient G1 checkpoint activation...
  49. Guo X, Hum Y, Lehner K, Jinks Robertson S. Regulation of hetDNA Length during Mitotic Double-Strand Break Repair in Yeast. Mol Cell. 2017;67:539-549.e4 pubmed publisher
    ..Loss of either the Exo1 or Sgs1 long-range resection pathway significantly shortened hetDNA, suggesting that these pathways normally ..
  50. Grandin N, Charbonneau M. RPA provides checkpoint-independent cell cycle arrest and prevents recombination at uncapped telomeres of Saccharomyces cerevisiae. DNA Repair (Amst). 2013;12:212-26 pubmed publisher
    ..In conclusion, the present study identifies a novel pathway of telomere end protection that utilizes a previously unsuspected function of RPA at the telomeres. ..
  51. Choudhury S, Asefa B, Kauler P, Chow T. Synergistic effect of TRM2/RNC1 and EXO1 in DNA double-strand break repair in Saccharomyces cerevisiae. Mol Cell Biochem. 2007;304:127-34 pubmed
    ..at the MAT locus by HO-endonuclease with higher sterility rate among the survivors compared to wild type (wt) or exo1 single mutants...
  52. Tran H, Gordenin D, Resnick M. The 3'-->5' exonucleases of DNA polymerases delta and epsilon and the 5'-->3' exonuclease Exo1 have major roles in postreplication mutation avoidance in Saccharomyces cerevisiae. Mol Cell Biol. 1999;19:2000-7 pubmed
    ..We have genetically characterized the roles of the 5'-->3' Exo1 and the 3'-->5' DNA polymerase exonucleases in mismatch repair in the yeast Saccharomyces cerevisiae by using ..
  53. Colosio A, Frattini C, Pellicanò G, Villa Hernández S, Bermejo R. Nucleolytic processing of aberrant replication intermediates by an Exo1-Dna2-Sae2 axis counteracts fork collapse-driven chromosome instability. Nucleic Acids Res. 2016;44:10676-10690 pubmed
    ..This revealed a novel role for Exo1 nuclease in resecting reversed replication fork structures and counteracting the accumulation of aberrant ..
  54. Dewar J, Lydall D. Pif1- and Exo1-dependent nucleases coordinate checkpoint activation following telomere uncapping. EMBO J. 2010;29:4020-34 pubmed publisher
    ..the telomere capping protein Cdc13 protects telomeres from the activity of the helicase Pif1 and the exonuclease Exo1. Our data support a two-stage model for the DDR at uncapped telomeres; Pif1 and Exo1 resect telomeric DNA <5 kb ..
  55. Karanja K, Livingston D. C-terminal flap endonuclease (rad27) mutations: lethal interactions with a DNA ligase I mutation (cdc9-p) and suppression by proliferating cell nuclear antigen (POL30) in Saccharomyces cerevisiae. Genetics. 2009;183:63-78 pubmed publisher
  56. Bernstein K, Mimitou E, Mihalevic M, Chen H, Sunjaveric I, Symington L, et al. Resection activity of the Sgs1 helicase alters the affinity of DNA ends for homologous recombination proteins in Saccharomyces cerevisiae. Genetics. 2013;195:1241-51 pubmed publisher
    ..in the absence of the Sae2 nuclease causes slow growth, which is alleviated by making the DNA ends accessible to Exo1 nuclease...
  57. Argueso J, Smith D, Yi J, Waase M, Sarin S, Alani E. Analysis of conditional mutations in the Saccharomyces cerevisiae MLH1 gene in mismatch repair and in meiotic crossing over. Genetics. 2002;160:909-21 pubmed
    ..The mutator phenotype exhibited in mlh1-I296S strains was partially suppressed at 35 degrees by EXO1 overexpression...
  58. Gobbini E, Villa M, Gnugnoli M, Menin L, Clerici M, Longhese M. Sae2 Function at DNA Double-Strand Breaks Is Bypassed by Dampening Tel1 or Rad53 Activity. PLoS Genet. 2015;11:e1005685 pubmed publisher
    ..We propose that persistent Tel1 and Rad53 checkpoint signaling in cells lacking Sae2 increases the association of Rad9 at DSBs, which in turn inhibits DSB resection by limiting the activity of the Sgs1-Dna2 resection machinery. ..
  59. Kaniak Golik A, Kuberska R, Dzierzbicki P, Sledziewska Gojska E. Activation of Dun1 in response to nuclear DNA instability accounts for the increase in mitochondrial point mutations in Rad27/FEN1 deficient S. cerevisiae. PLoS ONE. 2017;12:e0180153 pubmed publisher
    ..In addition, we have established that overproduction of Exo1, which suppresses DNA damage sensitivity and replication stress in nuclei of Rad27 deficient cells, but does not ..
  60. Bertrand P, Tishkoff D, Filosi N, Dasgupta R, Kolodner R. Physical interaction between components of DNA mismatch repair and nucleotide excision repair. Proc Natl Acad Sci U S A. 1998;95:14278-83 pubmed
    ..These data suggest that MSH2 and possibly other components of DNA mismatch repair exist in a complex with NER proteins, providing a biochemical and genetical basis for these proteins to function in common processes. ..