ERG6

Summary

Gene Symbol: ERG6
Description: sterol 24-C-methyltransferase
Alias: ISE1, LIS1, SED6, VID1, sterol 24-C-methyltransferase
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Bagnat M, Simons K. Cell surface polarization during yeast mating. Proc Natl Acad Sci U S A. 2002;99:14183-8 pubmed
    ..Our results show that membrane microdomain clustering at the mating projection is involved in the generation and maintenance of polarity during mating. ..
  2. Munn A, Heese Peck A, Stevenson B, Pichler H, Riezman H. Specific sterols required for the internalization step of endocytosis in yeast. Mol Biol Cell. 1999;10:3943-57 pubmed
    ..A single desaturation at C-8,9 was not sufficient to support internalization at 37 degrees C whereas two double bonds, either at C-5,6 and C-7,8 or at C-5,6 and C-8,9, allowed internalization. ..
  3. Gaber R, Copple D, Kennedy B, Vidal M, Bard M. The yeast gene ERG6 is required for normal membrane function but is not essential for biosynthesis of the cell-cycle-sparking sterol. Mol Cell Biol. 1989;9:3447-56 pubmed
    ..cerevisiae was genetically altered to assess the functional role of the C-28 methyl group of ergosterol. ERG6, the putative structural gene for S-adenosylmethionine: delta 24-methyltransferase, which catalyzes C-24 ..
  4. Markus S, Punch J, Lee W. Motor- and tail-dependent targeting of dynein to microtubule plus ends and the cell cortex. Curr Biol. 2009;19:196-205 pubmed publisher
    ..sites, whereas the C-terminal motor domain targets Dyn1/HC to microtubule plus ends in a Bik1/CLIP-170- and Pac1/LIS1-dependent manner...
  5. Tedrick K, Trischuk T, Lehner R, Eitzen G. Enhanced membrane fusion in sterol-enriched vacuoles bypasses the Vrp1p requirement. Mol Biol Cell. 2004;15:4609-21 pubmed
    ..Here we show that overexpression of ERG6, a gene involved in ergosterol synthesis, elevates sterol levels 1...
  6. Heese Peck A, Pichler H, Zanolari B, Watanabe R, Daum G, Riezman H. Multiple functions of sterols in yeast endocytosis. Mol Biol Cell. 2002;13:2664-80 pubmed
    ..Interestingly, sterol structures unable to support endocytosis allowed transport of the glycosylphosphatidylinositol-anchored protein Gas1p from the endoplasmic reticulum to Golgi compartment. ..
  7. Lone M, Atkinson A, Hodge C, Cottier S, Martínez Montañés F, Maithel S, et al. Yeast Integral Membrane Proteins Apq12, Brl1, and Brr6 Form a Complex Important for Regulation of Membrane Homeostasis and Nuclear Pore Complex Biogenesis. Eukaryot Cell. 2015;14:1217-27 pubmed publisher
    ..We suggest that the defects in nuclear pore complex biogenesis and mRNA export seen in these mutants are consequences of defects in maintaining the biophysical properties of the NE. ..
  8. Marshall R, McLoughlin F, Vierstra R. Autophagic Turnover of Inactive 26S Proteasomes in Yeast Is Directed by the Ubiquitin Receptor Cue5 and the Hsp42 Chaperone. Cell Rep. 2016;16:1717-1732 pubmed publisher
    ..Together, Cue5 and Hsp42 provide a quality control checkpoint in yeast directed at recycling dysfunctional 26S proteasomes. ..
  9. Currie E, Guo X, Christiano R, Chitraju C, Kory N, Harrison K, et al. High confidence proteomic analysis of yeast LDs identifies additional droplet proteins and reveals connections to dolichol synthesis and sterol acetylation. J Lipid Res. 2014;55:1465-77 pubmed publisher
    ..Our results provide a highly specific list of yeast LD proteins and reveal that the vast majority of these proteins are involved in lipid metabolism. ..

More Information

Publications49

  1. Athenstaedt K, Zweytick D, Jandrositz A, Kohlwein S, Daum G. Identification and characterization of major lipid particle proteins of the yeast Saccharomyces cerevisiae. J Bacteriol. 1999;181:6441-8 pubmed
    ..Strains with YBR177c, YMR313c, and YKL140w deleted exhibited phospholipid and/or neutral lipid patterns that were different from the wild-type strain and thus may be further candidate ORFs involved in yeast lipid metabolism. ..
  2. Valachovic M, Bareither B, Shah Alam Bhuiyan M, Eckstein J, Barbuch R, Balderes D, et al. Cumulative mutations affecting sterol biosynthesis in the yeast Saccharomyces cerevisiae result in synthetic lethality that is suppressed by alterations in sphingolipid profiles. Genetics. 2006;173:1893-908 pubmed
    ..addition we demonstrate that strains containing upc2Delta ecm22Delta are also inviable when carrying deletions of ERG6 and ERG28 but not when carrying deletions of ERG3, ERG4, or ERG5...
  3. Mo C, Bard M. A systematic study of yeast sterol biosynthetic protein-protein interactions using the split-ubiquitin system. Biochim Biophys Acta. 2005;1737:152-60 pubmed
    ..Our results provide a working model as to how sterol biosynthetic enzymes are topologically organized not only in yeast but in plant and animal systems that share many of these biosynthetic reactions. ..
  4. Walsh L, Hastwell P, Keenan P, Knight A, Billinton N, Walmsley R. Genetic modification and variations in solvent increase the sensitivity of the yeast RAD54-GFP genotoxicity assay. Mutagenesis. 2005;20:317-27 pubmed
    ..strain by deletion of genes involved in cell wall integrity or with products functioning as efflux pumps (PDR5, ERG6, SNQ2, YOR1); (ii) expression in the host yeast of human phase I metabolic activation genes and (iii) variation in ..
  5. Muthusamy B, Raychaudhuri S, Natarajan P, Abe F, Liu K, Prinz W, et al. Control of protein and sterol trafficking by antagonistic activities of a type IV P-type ATPase and oxysterol binding protein homologue. Mol Biol Cell. 2009;20:2920-31 pubmed publisher
  6. te Welscher Y, ten Napel H, Balagué M, Souza C, Riezman H, de Kruijff B, et al. Natamycin blocks fungal growth by binding specifically to ergosterol without permeabilizing the membrane. J Biol Chem. 2008;283:6393-401 pubmed publisher
    ..Also, in ergosterol containing model membranes, natamycin did not cause a change in bilayer permeability. This demonstrates that natamycin acts via a novel mode of action and blocks fungal growth by binding specifically to ergosterol. ..
  7. Huang Z, Chen K, Zhang J, Li Y, Wang H, Cui D, et al. A functional variomics tool for discovering drug-resistance genes and drug targets. Cell Rep. 2013;3:577-85 pubmed publisher
    ..New genes and alleles that confer resistance to other stresses can also be discovered. Similar tools in other systems, such as human cell lines, will also be useful. ..
  8. Chang H, Jesch S, Gaspar M, Henry S. Role of the unfolded protein response pathway in secretory stress and regulation of INO1 expression in Saccharomyces cerevisiae. Genetics. 2004;168:1899-913 pubmed
    ..Indeed, many of the Sec(-) mutants that had elevated UPR expression at semipermissive growth temperatures failed to achieve wild-type levels of INO1 expression under these same conditions. ..
  9. Mantzouridou F, Tsimidou M. Observations on squalene accumulation in Saccharomyces cerevisiae due to the manipulation of HMG2 and ERG6. FEMS Yeast Res. 2010;10:699-707 pubmed publisher
    ..stably integrated into the chromosomal HO locus, and AM64, a derivative of AM63 with an additional deletion of the ERG6 gene, were used as tools to test the squalene accumulation capacity of Saccharomyces cerevisiae...
  10. Umebayashi K, Nakano A. Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J Cell Biol. 2003;161:1117-31 pubmed
    It was known that the uptake of tryptophan is reduced in the yeast erg6 mutant, which is defective in a late step of ergosterol biosynthesis...
  11. Jin H, McCaffery J, Grote E. Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast. J Cell Biol. 2008;180:813-26 pubmed publisher
    ..In contrast to signaling, plasma membrane fusion is relatively insensitive to sphingolipid depletion. Thus, the sphingolipid-free pool of ergosterol promotes plasma membrane fusion. ..
  12. Daicho K, Makino N, Hiraki T, Ueno M, Uritani M, Abe F, et al. Sorting defects of the tryptophan permease Tat2 in an erg2 yeast mutant. FEMS Microbiol Lett. 2009;298:218-27 pubmed publisher
    ..When the activity of Erg9 involved in the first step of ergosterol biogenesis, but not that of Erg6 involved in a late step, is compromised, vacuolar degradation of the tryptophan permease Tat2 is promoted...
  13. Guan X, Souza C, Pichler H, Dewhurst G, Schaad O, Kajiwara K, et al. Functional interactions between sphingolipids and sterols in biological membranes regulating cell physiology. Mol Biol Cell. 2009;20:2083-95 pubmed publisher
  14. Mo C, Bard M. Erg28p is a key protein in the yeast sterol biosynthetic enzyme complex. J Lipid Res. 2005;46:1991-8 pubmed
    ..Based on these results, we suggest that many if not all sterol biosynthetic proteins may be tethered as a large complex. ..
  15. Wood M, Rawe M, Johansson G, Pang S, Soderquist R, Patel A, et al. Discovery of a small molecule targeting IRA2 deletion in budding yeast and neurofibromin loss in malignant peripheral nerve sheath tumor cells. Mol Cancer Ther. 2011;10:1740-50 pubmed publisher
    ..This work identifies a lead compound and a possible target pathway for NF1-associated MPNST, and shows a novel model system approach to identify and validate target pathways for cancer cells in which NF1 loss drives tumor formation. ..
  16. Leber R, Landl K, Zinser E, Ahorn H, Spök A, Kohlwein S, et al. Dual localization of squalene epoxidase, Erg1p, in yeast reflects a relationship between the endoplasmic reticulum and lipid particles. Mol Biol Cell. 1998;9:375-86 pubmed
    ..Close contact between lipid particles and endoplasmic reticulum may be necessary for a concerted action of these two compartments in sterol biosynthesis. ..
  17. Shah Alam Bhuiyan M, Eckstein J, Barbuch R, Bard M. Synthetically lethal interactions involving loss of the yeast ERG24: the sterol C-14 reductase gene. Lipids. 2007;42:69-76 pubmed
    ..Further genetic analysis indicates that erg24 combined with mutations in erg6 or erg28 is synthetically lethal but when combined with mutations in erg3 is weakly viable...
  18. Toropova K, Zou S, Roberts A, Redwine W, Goodman B, Reck Peterson S, et al. Lis1 regulates dynein by sterically blocking its mechanochemical cycle. elife. 2014;3: pubmed publisher
    ..The dynein regulator Lis1 is known to keep dynein bound to microtubules; however, how this is accomplished mechanistically remains unknown...
  19. Grote E. Secretion is required for late events in the cell-fusion pathway of mating yeast. J Cell Sci. 2010;123:1902-12 pubmed publisher
  20. Desantis M, Cianfrocco M, Htet Z, Tran P, Reck Peterson S, Leschziner A. Lis1 Has Two Opposing Modes of Regulating Cytoplasmic Dynein. Cell. 2017;170:1197-1208.e12 pubmed publisher
    ..Previous work established that Lis1, a conserved regulator of dynein, binds to its motor domain and induces a tight microtubule-binding state in dynein...
  21. Sitcheran R, Emter R, Kralli A, Yamamoto K. A genetic analysis of glucocorticoid receptor signaling. Identification and characterization of ligand-effect modulators in Saccharomyces cerevisiae. Genetics. 2000;156:963-72 pubmed
    ..These studies identify two genes, LEM3 and LEM4, which correspond to YNL323w and ERG6, respectively...
  22. Jayasimha P, Nes W. Photoaffinity labeling and mutational analysis of 24-C-sterol methyltransferase defines the AdoMet binding site. Lipids. 2008;43:681-93 pubmed publisher
    ..The results indicate that one or both of Cys128 and Pro133 are covalently bound to AdoMet. ..
  23. Chiabudini M, Conz C, Reckmann F, Rospert S. Ribosome-associated complex and Ssb are required for translational repression induced by polylysine segments within nascent chains. Mol Cell Biol. 2012;32:4769-79 pubmed publisher
    ..This active role of RAC/Ssb in the quality control of polylysine proteins significantly contributed to the low level of expression of nonstop transcripts in vivo. ..
  24. Pichler H, Gaigg B, Hrastnik C, Achleitner G, Kohlwein S, Zellnig G, et al. A subfraction of the yeast endoplasmic reticulum associates with the plasma membrane and has a high capacity to synthesize lipids. Eur J Biochem. 2001;268:2351-61 pubmed
    ..A possible role of PAM in the supply of lipids to the plasma membrane is discussed. ..
  25. McCammon M, Hartmann M, Bottema C, Parks L. Sterol methylation in Saccharomyces cerevisiae. J Bacteriol. 1984;157:475-83 pubmed
    ..1.1.41) were shown to possess alleles of the same gene, erg6. The genetic map location of erg6 was shown to be close to trp1 on chromosome 4...
  26. Olmo V, Grote E. Prm1 targeting to contact sites enhances fusion during mating in Saccharomyces cerevisiae. Eukaryot Cell. 2010;9:1538-48 pubmed publisher
    ..Thus, the amount of Prm1 expressed in mating yeast is far in excess of the amount required to facilitate fusion. ..
  27. Eisenkolb M, Zenzmaier C, Leitner E, Schneiter R. A specific structural requirement for ergosterol in long-chain fatty acid synthesis mutants important for maintaining raft domains in yeast. Mol Biol Cell. 2002;13:4414-28 pubmed
    ..of yeast, ergosterol, becomes essential, because mutations in ELO3 are synthetically lethal with mutations in ERG6. Erg6p catalyzes the methylation of carbon atom 24 in the aliphatic side chain of sterol...
  28. Roberts A, Goodman B, Reck Peterson S. Reconstitution of dynein transport to the microtubule plus end by kinesin. elife. 2014;3:e02641 pubmed publisher
    ..cerevisiae and dissect the mechanism using single-molecule microscopy. We find that two proteins-homologs of Lis1 and Clip170-are sufficient to couple dynein to Kip2, a plus-end-directed kinesin...
  29. Hodge C, Choudhary V, Wolyniak M, Scarcelli J, Schneiter R, Cole C. Integral membrane proteins Brr6 and Apq12 link assembly of the nuclear pore complex to lipid homeostasis in the endoplasmic reticulum. J Cell Sci. 2010;123:141-51 pubmed publisher
    ..These data indicate that Brr6 has an essential function in regulating lipid homeostasis in the NE-ER, thereby impacting NPC formation and nucleocytoplasmic transport. ..
  30. Khoury C, Yang Z, Li X, Vignali M, Fields S, Greenwood M. A TSC22-like motif defines a novel antiapoptotic protein family. FEMS Yeast Res. 2008;8:540-63 pubmed publisher
    ..Overexpression and knock-out experiments were used to validate this prediction. These findings demonstrate the potential of studying heterologous proteins in yeast to uncover novel biological insights into the regulation of apoptosis. ..
  31. Ueno K, Nagano M, Shimizu S, Toshima J, Toshima J. Lipid droplet proteins, Lds1p, Lds2p, and Rrt8p, are implicated in membrane protein transport associated with ergosterol. Biochem Biophys Res Commun. 2016;475:315-21 pubmed publisher
    ..These results suggest a possible role of Lds proteins in maintenance of membrane lipid homeostasis and accompanying membrane protein transport. ..
  32. Aresta Branco F, Cordeiro A, Marinho H, Cyrne L, Antunes F, de Almeida R. Gel domains in the plasma membrane of Saccharomyces cerevisiae: highly ordered, ergosterol-free, and sphingolipid-enriched lipid rafts. J Biol Chem. 2011;286:5043-54 pubmed publisher
    ..The abundance of the sphingolipid-enriched gel domains was inversely related to the cellular membrane system global order, suggesting their involvement in the regulation of membrane properties. ..
  33. Cary G, Yoon S, Torres C, Wang K, Hays M, Ludlow C, et al. Identification and characterization of a drug-sensitive strain enables puromycin-based translational assays in Saccharomyces cerevisiae. Yeast. 2014;31:167-78 pubmed publisher
    ..Puromycin inhibits the growth of yeast cells mutant for erg6?, pdr1? and pdr3? (EPP) on both solid and liquid media...
  34. Emter R, Heese Peck A, Kralli A. ERG6 and PDR5 regulate small lipophilic drug accumulation in yeast cells via distinct mechanisms. FEBS Lett. 2002;521:57-61 pubmed
    ..In the model organism Saccharomyces cerevisiae, deletions of PDR5 or ERG6 increase sensitivity to many small lipophilic drugs...
  35. Zhang Z, He X, Li W, Lu Y, Wang Z, Zhang B. [Regulation role of sterol C-24 methyltransferase and sterol C-8 isomerase in the ergosterol biosynthesis of Saccharomyces cerevisiae]. Wei Sheng Wu Xue Bao. 2009;49:1063-8 pubmed
    ..In addition,the regulation role of sterol C-24 methyltransferase, encoded by ERG6, in ergosterol biosynthesis was further verified by analysis of sterol components and levels in yeast strains ..
  36. Souza C, Schwabe T, Pichler H, Ploier B, Leitner E, Guan X, et al. A stable yeast strain efficiently producing cholesterol instead of ergosterol is functional for tryptophan uptake, but not weak organic acid resistance. Metab Eng. 2011;13:555-69 pubmed publisher
    ..These strains will also be improved hosts for heterologous expression of sterol-dependent proteins and safe sources to obtain pure cholesterol and other sterols...
  37. Dupont S, Beney L, Ferreira T, Gervais P. Nature of sterols affects plasma membrane behavior and yeast survival during dehydration. Biochim Biophys Acta. 2011;1808:1520-8 pubmed publisher
    ..ultrastructure, cell volume, and membrane permeability) in the wild-type (WT) strain and the ergosterol mutant erg6? strain...
  38. Mo C, Valachovic M, Bard M. The ERG28-encoded protein, Erg28p, interacts with both the sterol C-4 demethylation enzyme complex as well as the late biosynthetic protein, the C-24 sterol methyltransferase (Erg6p). Biochim Biophys Acta. 2004;1686:30-6 pubmed
    ..We conclude that Erg28p may not only anchor the C-4 demethylation enzyme complex to the ER but also acts as a protein bridge to the Erg6p enzyme required for the next ergosterol biosynthetic step. ..
  39. Hongay C, Jia N, Bard M, Winston F. Mot3 is a transcriptional repressor of ergosterol biosynthetic genes and is required for normal vacuolar function in Saccharomyces cerevisiae. EMBO J. 2002;21:4114-24 pubmed
    ..Transcriptional analyses show that Mot3 represses transcription of ERG2, ERG6 and ERG9, genes required for ergosterol biosynthesis, during both aerobic and hypoxic growth...
  40. Heiderpriem R, Livant P, Parish E, Barbuch R, Broaddus M, Bard M. A simple method for the isolation of zymosterol from a sterol mutant of Saccharomyces cerevisiae. J Steroid Biochem Mol Biol. 1992;43:741-3 pubmed
    ..This yeast strain, which is a double mutant of the ERG6 (sterol transmethylase) and ERG2 (C-8 sterol isomerase) genes, accumulates zymosterol as its major sterol component. ..