Gene Symbol: ENO2
Description: phosphopyruvate hydratase ENO2
Alias: phosphopyruvate hydratase ENO2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Cohen R, Holland J, Yokoi T, Holland M. Identification of a regulatory region that mediates glucose-dependent induction of the Saccharomyces cerevisiae enolase gene ENO2. Mol Cell Biol. 1986;6:2287-97 pubmed
    There are two yeast enolase genes, designated ENO1 and ENO2, which are expressed differentially in vegetative cells grown on glucose and in cells grown on gluconeogenic carbon sources...
  2. de Nobel H, Lawrie L, Brul S, Klis F, Davis M, Alloush H, et al. Parallel and comparative analysis of the proteome and transcriptome of sorbic acid-stressed Saccharomyces cerevisiae. Yeast. 2001;18:1413-28 pubmed
    ..or Ssa2, Ssb1 or Ssb2, Ssc1, Ssa4, Ach1, Zwf1 and Tdh1; and the downregulation of three proteins; Ade16, Adh3 and Eno2. In parallel, of 6144 ORFs, 94 (1.53%) showed greater than a 1...
  3. López Villar E, Monteoliva L, Larsen M, Sachon E, Shabaz M, Pardo M, et al. Genetic and proteomic evidences support the localization of yeast enolase in the cell surface. Proteomics. 2006;6 Suppl 1:S107-18 pubmed
    ..Elongation factors, mitochondrial membrane proteins and a mannosyltransferase involved in cell wall mannan biosynthesis were also identified in this fraction. ..
  4. Decker B, Wickner W. Enolase activates homotypic vacuole fusion and protein transport to the vacuole in yeast. J Biol Chem. 2006;281:14523-8 pubmed
    ..Either deletion of the non-essential ENO1 gene or diminished expression of the essential ENO2 gene causes vacuole fragmentation in vivo, reflecting reduced fusion...
  5. McAlister L, Holland M. Targeted deletion of a yeast enolase structural gene. Identification and isolation of yeast enolase isozymes. J Biol Chem. 1982;257:7181-8 pubmed
    ..The yeast enolase loci have been designated ENO1 and ENO2. The deletion mutant lacks the enolase 1 polypeptide confirming that this polypeptide is encoded by the gene ..
  6. Bailleul P, Newnam G, Steenbergen J, Chernoff Y. Genetic study of interactions between the cytoskeletal assembly protein sla1 and prion-forming domain of the release factor Sup35 (eRF3) in Saccharomyces cerevisiae. Genetics. 1999;153:81-94 pubmed
    ..We hypothesize that Sup35N is involved in regulatory interactions with intracellular structural networks, and [PSI] prion may be formed as a by-product of this process. ..
  7. Kornblatt M, Richard Albert J, Mattie S, Zakaib J, Dayanandan S, Hanic Joyce P, et al. The Saccharomyces cerevisiae enolase-related regions encode proteins that are active enolases. Yeast. 2013;30:55-69 pubmed publisher
    In addition to two genes (ENO1 and ENO2) known to code for enolase (EC4.2.1...
  8. Lee J, Ishida Y, Kuge S, Naganuma A, Hwang G. Identification of substrates of F-box protein involved in methylmercury toxicity in yeast cells. FEBS Lett. 2015;589:2720-5 pubmed publisher
    ..In this study, we found that Dld3 and Grs1 bound to Hrt3 and that Eno2 bound to Ucc1 using a yeast two-hybrid screening...
  9. Baleva M, Meyer M, Entelis N, Tarassov I, Kamenski P, Masquida B. Factors beyond Enolase 2 and Mitochondrial Lysyl-tRNA Synthetase Precursor Are Required for tRNA Import into Yeast Mitochondria. Biochemistry (Mosc). 2017;82:1324-1335 pubmed publisher
    ..Taken together, our data suggest the existence of a protein complex containing Eno2p that is involved in RNA mitochondrial import. ..

More Information


  1. Brandina I, Graham J, Lemaitre Guillier C, Entelis N, Krasheninnikov I, Sweetlove L, et al. Enolase takes part in a macromolecular complex associated to mitochondria in yeast. Biochim Biophys Acta. 2006;1757:1217-28 pubmed
    ..This suggests an unsuspected novel function for this complex in tRNA mitochondrial import. ..
  2. Kaplan Y, Kupiec M. A role for the yeast cell cycle/splicing factor Cdc40 in the G1/S transition. Curr Genet. 2007;51:123-40 pubmed
    ..Finally, we discuss possible mechanisms of suppression by the cDNAs that imply cell cycle regulation by apparently unrelated processes, such as splicing, translation initiation and glycolysis. ..
  3. Guillen Ahlers H, Rao P, Levenstein M, Kennedy Darling J, Perumalla D, Jadhav A, et al. HyCCAPP as a tool to characterize promoter DNA-protein interactions in Saccharomyces cerevisiae. Genomics. 2016;107:267-73 pubmed publisher
    ..Both novel and known locus-specific DNA-protein interactions were identified at the ENO2 and GAL1 promoter regions of Saccharomyces cerevisiae, and revealed subgroups of proteins present in significantly ..
  4. Kornblatt M, Lange R, Balny C. Use of hydrostatic pressure to produce 'native' monomers of yeast enolase. Eur J Biochem. 2004;271:3897-904 pubmed
  5. Entelis N, Brandina I, Kamenski P, Krasheninnikov I, Martin R, Tarassov I. A glycolytic enzyme, enolase, is recruited as a cofactor of tRNA targeting toward mitochondria in Saccharomyces cerevisiae. Genes Dev. 2006;20:1609-20 pubmed
    ..Our results indicate an alternative molecular chaperone function of glycolytic enzyme enolase in tRNA mitochondrial targeting. ..