Gene Symbol: ENO1
Description: phosphopyruvate hydratase ENO1
Alias: HSP48, phosphopyruvate hydratase ENO1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. McAlister L, Holland M. Targeted deletion of a yeast enolase structural gene. Identification and isolation of yeast enolase isozymes. J Biol Chem. 1982;257:7181-8 pubmed
    ..The yeast enolase loci have been designated ENO1 and ENO2...
  2. Brandina I, Graham J, Lemaitre Guillier C, Entelis N, Krasheninnikov I, Sweetlove L, et al. Enolase takes part in a macromolecular complex associated to mitochondria in yeast. Biochim Biophys Acta. 2006;1757:1217-28 pubmed
    ..This suggests an unsuspected novel function for this complex in tRNA mitochondrial import. ..
  3. Decker B, Wickner W. Enolase activates homotypic vacuole fusion and protein transport to the vacuole in yeast. J Biol Chem. 2006;281:14523-8 pubmed
    ..Either deletion of the non-essential ENO1 gene or diminished expression of the essential ENO2 gene causes vacuole fragmentation in vivo, reflecting reduced ..
  4. Araiza Olivera D, Chiquete Felix N, Rosas Lemus M, Sampedro J, Pena A, M├║jica A, et al. A glycolytic metabolon in Saccharomyces cerevisiae is stabilized by F-actin. FEBS J. 2013;280:3887-905 pubmed publisher
    ..In S. cerevisiae, a glycolytic metabolon appear to assemble in association with F-actin. In this complex, fermentation activity is enhanced and enzymes are partially protected against inhibition by trehalose or by antibodies. ..
  5. Miura N, Shinohara M, Tatsukami Y, Sato Y, Morisaka H, Kuroda K, et al. Spatial reorganization of Saccharomyces cerevisiae enolase to alter carbon metabolism under hypoxia. Eukaryot Cell. 2013;12:1106-19 pubmed publisher
  6. Kornblatt M, Richard Albert J, Mattie S, Zakaib J, Dayanandan S, Hanic Joyce P, et al. The Saccharomyces cerevisiae enolase-related regions encode proteins that are active enolases. Yeast. 2013;30:55-69 pubmed publisher
    In addition to two genes (ENO1 and ENO2) known to code for enolase (EC4.2.1...
  7. Choi I, Jeong M, Ham M, Sung H, Yun C. Novel Ree1 regulates the expression of ENO1 via the Snf1 complex pathway in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2008;377:395-399 pubmed publisher
    ..Furthermore, from 2-D gel electrophoresis we found that the deletion of REE1 resulted in the up-regulation of Eno1. From Western blotting, we learned that the expression of Eno1 in the Deltaree1 strain was different from that in ..
  8. Gomes R, Oliveira L, Silva M, Ascenso C, Quintas A, Costa G, et al. Protein glycation in vivo: functional and structural effects on yeast enolase. Biochem J. 2008;416:317-26 pubmed publisher
    ..In vivo, glycation appears to be a specific process, where the same residues are consistently modified in the same way, whereas in vitro several residues are modified with different advanced glycation end-products. ..
  9. Kornblatt M, Lange R, Balny C. Use of hydrostatic pressure to produce 'native' monomers of yeast enolase. Eur J Biochem. 2004;271:3897-904 pubmed

More Information


  1. Hall L, Sanchez R, Holloway S, Zhu H, Stine J, Lyons T, et al. X-ray crystallographic and analytical ultracentrifugation analyses of truncated and full-length yeast copper chaperones for SOD (LYS7): a dimer-dimer model of LYS7-SOD association and copper delivery. Biochemistry. 2000;39:3611-23 pubmed
    ..The advantages of the dimer-dimer model over the heterodimer model are enumerated. ..
  2. Cohen R, Yokoi T, Holland J, Pepper A, Holland M. Transcription of the constitutively expressed yeast enolase gene ENO1 is mediated by positive and negative cis-acting regulatory sequences. Mol Cell Biol. 1987;7:2753-61 pubmed
    There are two enolase genes, ENO1 and ENO2, per haploid yeast genome. Expression of the ENO1 gene is quantitatively similar in cells grown on glucose or gluconeogenic carbon sources...
  3. Chen M, Lopes J. Multiple basic helix-loop-helix proteins regulate expression of the ENO1 gene of Saccharomyces cerevisiae. Eukaryot Cell. 2007;6:786-96 pubmed
    ..We tested the ability of all nine Saccharomyces cerevisiae bHLH proteins to regulate the enolase-encoding gene ENO1. ENO1 was known to be activated by the bHLH protein Sgc1p...
  4. Fan X, Martin Brown S, Florens L, Li R. Intrinsic capability of budding yeast cofilin to promote turnover of tropomyosin-bound actin filaments. PLoS ONE. 2008;3:e3641 pubmed publisher
    ..Our results suggest that yeast cofilin has the intrinsic ability to promote actin cable turnover, and that the severing activity may rely on its ability to bind Tpm1. ..
  5. Larsen T, Wedekind J, Rayment I, Reed G. A carboxylate oxygen of the substrate bridges the magnesium ions at the active site of enolase: structure of the yeast enzyme complexed with the equilibrium mixture of 2-phosphoglycerate and phosphoenolpyruvate at 1.8 A resolution. Biochemistry. 1996;35:4349-58 pubmed
    ..The structure provides a candid view of the catalytic machinery of enolase. ..
  6. Wedekind J, Poyner R, Reed G, Rayment I. Chelation of serine 39 to Mg2+ latches a gate at the active site of enolase: structure of the bis(Mg2+) complex of yeast enolase and the intermediate analog phosphonoacetohydroxamate at 2.1-A resolution. Biochemistry. 1994;33:9333-42 pubmed
    ..The position of the second Mg2+ in the active site provides new insight into the stereochemistry of substrate binding...
  7. Wedekind J, Reed G, Rayment I. Octahedral coordination at the high-affinity metal site in enolase: crystallographic analysis of the MgII--enzyme complex from yeast at 1.9 A resolution. Biochemistry. 1995;34:4325-30 pubmed
    ..These latter enzymes have alpha/beta-barrel folds comparable to enolase.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  8. Denison C, Rudner A, Gerber S, Bakalarski C, Moazed D, Gygi S. A proteomic strategy for gaining insights into protein sumoylation in yeast. Mol Cell Proteomics. 2005;4:246-54 pubmed
    ..These data combine with recent works to further our understanding of the breadth and impact of protein sumoylation in a diverse array of biological processes. ..
  9. Zhou W, Ryan J, Zhou H. Global analyses of sumoylated proteins in Saccharomyces cerevisiae. Induction of protein sumoylation by cellular stresses. J Biol Chem. 2004;279:32262-8 pubmed
    ..Taken together, these results show that protein sumoylation is broadly involved in many cellular functions and this mass spectrometry-based proteomic approach is useful in studying the regulation of protein sumoylation in the cells. ..
  10. Zhao S, Choy B, Kornblatt M. Effects of the G376E and G157D mutations on the stability of yeast enolase--a model for human muscle enolase deficiency. FEBS J. 2008;275:97-106 pubmed
    ..The identification of the cleavage sites and spectral studies of enolase have revealed some of the structural differences between the dimeric and monomeric forms of this enzyme. ..
  11. Zhang E, Brewer J, Minor W, Carreira L, Lebioda L. Mechanism of enolase: the crystal structure of asymmetric dimer enolase-2-phospho-D-glycerate/enolase-phosphoenolpyruvate at 2.0 A resolution. Biochemistry. 1997;36:12526-34 pubmed
    ..The crystals are unique because they have resolved two intermediates on the opposite sides of the transition state. ..
  12. Paludo G, Lorenzatto K, Bonatto D, Ferreira H. Systems biology approach reveals possible evolutionarily conserved moonlighting functions for enolase. Comput Biol Chem. 2015;58:1-8 pubmed publisher
    ..Overall, our results provided evidences of enolase multifunctionality and evolutionary conservation of enolase PPIs at all these levels. ..