Genomes and Genes
Gene Symbol: ENO1
Description: phosphopyruvate hydratase ENO1
Alias: HSP48, phosphopyruvate hydratase ENO1
Species: Saccharomyces cerevisiae S288c
- Hall L, Sanchez R, Holloway S, Zhu H, Stine J, Lyons T, et al. X-ray crystallographic and analytical ultracentrifugation analyses of truncated and full-length yeast copper chaperones for SOD (LYS7): a dimer-dimer model of LYS7-SOD association and copper delivery. Biochemistry. 2000;39:3611-23 pubmed..The advantages of the dimer-dimer model over the heterodimer model are enumerated. ..
- Cohen R, Yokoi T, Holland J, Pepper A, Holland M. Transcription of the constitutively expressed yeast enolase gene ENO1 is mediated by positive and negative cis-acting regulatory sequences. Mol Cell Biol. 1987;7:2753-61 pubmedThere are two enolase genes, ENO1 and ENO2, per haploid yeast genome. Expression of the ENO1 gene is quantitatively similar in cells grown on glucose or gluconeogenic carbon sources...
- Chen M, Lopes J. Multiple basic helix-loop-helix proteins regulate expression of the ENO1 gene of Saccharomyces cerevisiae. Eukaryot Cell. 2007;6:786-96 pubmed..We tested the ability of all nine Saccharomyces cerevisiae bHLH proteins to regulate the enolase-encoding gene ENO1. ENO1 was known to be activated by the bHLH protein Sgc1p...
- Fan X, Martin Brown S, Florens L, Li R. Intrinsic capability of budding yeast cofilin to promote turnover of tropomyosin-bound actin filaments. PLoS ONE. 2008;3:e3641 pubmed publisher..Our results suggest that yeast cofilin has the intrinsic ability to promote actin cable turnover, and that the severing activity may rely on its ability to bind Tpm1. ..
- Larsen T, Wedekind J, Rayment I, Reed G. A carboxylate oxygen of the substrate bridges the magnesium ions at the active site of enolase: structure of the yeast enzyme complexed with the equilibrium mixture of 2-phosphoglycerate and phosphoenolpyruvate at 1.8 A resolution. Biochemistry. 1996;35:4349-58 pubmed..The structure provides a candid view of the catalytic machinery of enolase. ..
- Wedekind J, Poyner R, Reed G, Rayment I. Chelation of serine 39 to Mg2+ latches a gate at the active site of enolase: structure of the bis(Mg2+) complex of yeast enolase and the intermediate analog phosphonoacetohydroxamate at 2.1-A resolution. Biochemistry. 1994;33:9333-42 pubmed..The position of the second Mg2+ in the active site provides new insight into the stereochemistry of substrate binding...
- Wedekind J, Reed G, Rayment I. Octahedral coordination at the high-affinity metal site in enolase: crystallographic analysis of the MgII--enzyme complex from yeast at 1.9 A resolution. Biochemistry. 1995;34:4325-30 pubmed..These latter enzymes have alpha/beta-barrel folds comparable to enolase.(ABSTRACT TRUNCATED AT 250 WORDS) ..
- Denison C, Rudner A, Gerber S, Bakalarski C, Moazed D, Gygi S. A proteomic strategy for gaining insights into protein sumoylation in yeast. Mol Cell Proteomics. 2005;4:246-54 pubmed..These data combine with recent works to further our understanding of the breadth and impact of protein sumoylation in a diverse array of biological processes. ..
- Zhou W, Ryan J, Zhou H. Global analyses of sumoylated proteins in Saccharomyces cerevisiae. Induction of protein sumoylation by cellular stresses. J Biol Chem. 2004;279:32262-8 pubmed..Taken together, these results show that protein sumoylation is broadly involved in many cellular functions and this mass spectrometry-based proteomic approach is useful in studying the regulation of protein sumoylation in the cells. ..
- Zhao S, Choy B, Kornblatt M. Effects of the G376E and G157D mutations on the stability of yeast enolase--a model for human muscle enolase deficiency. FEBS J. 2008;275:97-106 pubmed..The identification of the cleavage sites and spectral studies of enolase have revealed some of the structural differences between the dimeric and monomeric forms of this enzyme. ..
- Zhang E, Brewer J, Minor W, Carreira L, Lebioda L. Mechanism of enolase: the crystal structure of asymmetric dimer enolase-2-phospho-D-glycerate/enolase-phosphoenolpyruvate at 2.0 A resolution. Biochemistry. 1997;36:12526-34 pubmed..The crystals are unique because they have resolved two intermediates on the opposite sides of the transition state. ..
- Paludo G, Lorenzatto K, Bonatto D, Ferreira H. Systems biology approach reveals possible evolutionarily conserved moonlighting functions for enolase. Comput Biol Chem. 2015;58:1-8 pubmed publisher..Overall, our results provided evidences of enolase multifunctionality and evolutionary conservation of enolase PPIs at all these levels. ..