Gene Symbol: EMP47
Description: Emp47p
Alias: Emp47p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Spang A, Schekman R. Reconstitution of retrograde transport from the Golgi to the ER in vitro. J Cell Biol. 1998;143:589-99 pubmed
    ..Putative retrograde carriers (COPI vesicles) generated from Golgi-enriched membranes contain v-SNAREs as well as Emp47p as cargo.
  2. Mesecke N, Spang A, Deponte M, Herrmann J. A novel group of glutaredoxins in the cis-Golgi critical for oxidative stress resistance. Mol Biol Cell. 2008;19:2673-80 pubmed publisher
    ..Our observations suggest that Grx6 and Grx7 do not play a general role in the oxidative folding of proteins in the early secretory pathway but rather counteract the oxidation of specific thiol groups in substrate proteins. ..
  3. Sato K, Nakano A. Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae. Mol Biol Cell. 2002;13:2518-32 pubmed
    The yeast open reading frame YLR080w/EMP46 encodes a homolog of the Golgi protein Emp47p. These two proteins are 45% identical and have a single transmembrane domain in their C-terminal regions and a carbohydrate recognition domain ..
  4. Zink S, Wenzel D, Wurm C, Schmitt H. A link between ER tethering and COP-I vesicle uncoating. Dev Cell. 2009;17:403-16 pubmed publisher
  5. Ishii K, Enda H, Noda M, Kajino M, Kim A, Kurimoto E, et al. pH-Dependent Assembly and Segregation of the Coiled-Coil Segments of Yeast Putative Cargo Receptors Emp46p and Emp47p. PLoS ONE. 2015;10:e0140287 pubmed publisher
    Emp46p and Emp47p are yeast putative cargo receptors that recycle between the endoplasmic reticulum and the Golgi apparatus. These receptors can form complexes in a pH-dependent manner, but their molecular mechanisms remain unclear...
  6. Dilcher M, Veith B, Chidambaram S, Hartmann E, Schmitt H, Fischer von Mollard G. Use1p is a yeast SNARE protein required for retrograde traffic to the ER. EMBO J. 2003;22:3664-74 pubmed
    ..Therefore, we conclude that Use1p is a novel SNARE protein that functions in retrograde traffic from the Golgi to the ER. ..
  7. Eugster A, Frigerio G, Dale M, Duden R. The alpha- and beta'-COP WD40 domains mediate cargo-selective interactions with distinct di-lysine motifs. Mol Biol Cell. 2004;15:1011-23 pubmed
    ..Here, we show that the WD40 domain of beta'-COP is required for the recycling of the KTKLL-tagged Golgi protein Emp47p. The protein is degraded more rapidly in cells with a point mutation in the WD40 domain of beta'-COP (sec27-95) or ..
  8. Satoh T, Sato K, Kanoh A, Yamashita K, Yamada Y, Igarashi N, et al. Structures of the carbohydrate recognition domain of Ca2+-independent cargo receptors Emp46p and Emp47p. J Biol Chem. 2006;281:10410-9 pubmed
    Emp46p and Emp47p are type I membrane proteins, which cycle between the endoplasmic reticulum (ER) and the Golgi apparatus by vesicles coated with coat protein complexes I and II (COPI and COPII)...
  9. Todorow Z, Spang A, Carmack E, Yates J, Schekman R. Active recycling of yeast Golgi mannosyltransferase complexes through the endoplasmic reticulum. Proc Natl Acad Sci U S A. 2000;97:13643-8 pubmed
    ..These observations indicate that the Mnn9p-containing mannosyltransferase complexes cycle back and forth between the ER and Golgi. ..

More Information


  1. Otte S, Belden W, Heidtman M, Liu J, Jensen O, Barlowe C. Erv41p and Erv46p: new components of COPII vesicles involved in transport between the ER and Golgi complex. J Cell Biol. 2001;152:503-18 pubmed
    ..Emp24p, and Erv25p) and an additional nine species (Yip3p, Rer1p, Erp1p, Erp2p, Erv29p, Yif1p, Erv41p, Erv46p, and Emp47p) that had not been localized to ER vesicles...
  2. Sato K, Nakano A. Oligomerization of a cargo receptor directs protein sorting into COPII-coated transport vesicles. Mol Biol Cell. 2003;14:3055-63 pubmed
    ..Here we show that Emp47p, a type-I membrane protein, is specifically required for the transport of an integral membrane protein, Emp46p, ..
  3. Sato K, Nakano A. Reconstitution of coat protein complex II (COPII) vesicle formation from cargo-reconstituted proteoliposomes reveals the potential role of GTP hydrolysis by Sar1p in protein sorting. J Biol Chem. 2004;279:1330-5 pubmed
    ..b>Emp47p, a type-I membrane protein, is specifically required for the transport of an integral membrane protein, Emp46p, ..
  4. Denic V, Quan E, Weissman J. A luminal surveillance complex that selects misfolded glycoproteins for ER-associated degradation. Cell. 2006;126:349-59 pubmed
  5. Matiach A, Schröder Köhne S. Yeast cys3 and gsh1 mutant cells display overlapping but non-identical symptoms of oxidative stress with regard to subcellular protein localization and CDP-DAG metabolism. Mol Genet Genomics. 2001;266:481-96 pubmed
    ..of Saccharomyces cerevisiae that are defective in the proper localization of the Golgi transmembrane protein Emp47p, we uncovered a constitutive loss-of-function mutation in CYS3/STR1, the gene coding for cystathionine-gamma-lyase...
  6. Schindler C, Rodriguez F, Poon P, Singer R, Johnston G, Spang A. The GAP domain and the SNARE, coatomer and cargo interaction region of the ArfGAP2/3 Glo3 are sufficient for Glo3 function. Traffic. 2009;10:1362-75 pubmed publisher
    ..Our data suggest that membrane-interaction modules and cargo-sensing regions have evolved independently in ArfGAP1s versus ArfGAP2/3s. ..