ELP6

Summary

Gene Symbol: ELP6
Description: Elongator subunit ELP6
Alias: HAP3, KTI4, TOT6, Elongator subunit ELP6
Species: Saccharomyces cerevisiae S288c
Products:     ELP6

Top Publications

  1. Lin Z, Zhao W, Diao W, Xie X, Wang Z, Zhang J, et al. Crystal structure of elongator subcomplex Elp4-6. J Biol Chem. 2012;287:21501-8 pubmed publisher
    ..The overall structure of Elp4-6 revealed that Elp6 acts as a bridge to assemble Elp4 and Elp5...
  2. Glatt S, Létoquart J, Faux C, Taylor N, Seraphin B, Müller C. The Elongator subcomplex Elp456 is a hexameric RecA-like ATPase. Nat Struct Mol Biol. 2012;19:314-20 pubmed publisher
    ..Our results support a role of Elongator in tRNA modification, explain the importance of each of the Elp4, Elp5 and Elp6 subunits for complex integrity and suggest a model for the overall architecture of the holo-Elongator complex.
  3. Daudén M, Kosinski J, Kolaj Robin O, Desfosses A, Ori A, Faux C, et al. Architecture of the yeast Elongator complex. EMBO Rep. 2017;18:264-279 pubmed publisher
    ..Our study provides a structural framework on how the tRNA modification activity is carried out by Elongator. ..
  4. Xu H, Lin Z, Li F, Diao W, Dong C, Zhou H, et al. Dimerization of elongator protein 1 is essential for Elongator complex assembly. Proc Natl Acad Sci U S A. 2015;112:10697-702 pubmed publisher
  5. Xu H, Bygdell J, Wingsle G, Byström A. Yeast Elongator protein Elp1p does not undergo proteolytic processing in exponentially growing cells. Microbiologyopen. 2015;4:867-78 pubmed publisher
    ..Consequently, our results indicate that N-terminal truncation of Elp1p is not likely to regulate Elongator complex activity. ..
  6. Goehring A, Rivers D, Sprague G. Urmylation: a ubiquitin-like pathway that functions during invasive growth and budding in yeast. Mol Biol Cell. 2003;14:4329-41 pubmed
    ..Loss of five genes that are also essential in a cla4Delta strain, NCS2, NCS6, ELP2, ELP6, and URE2, affect the level of at least one Urm1p conjugate...
  7. Winkler G, Petrakis T, Ethelberg S, Tokunaga M, Erdjument Bromage H, Tempst P, et al. RNA polymerase II elongator holoenzyme is composed of two discrete subcomplexes. J Biol Chem. 2001;276:32743-9 pubmed
    ..identified Elp1, Elp2, and Elp3 proteins and another comprised of three novel polypeptides, termed Elp4, Elp5, and Elp6. Disruption of the yeast genes encoding the new Elongator proteins confers phenotypes indistinguishable from those ..
  8. Huang B, Lu J, Byström A. A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. RNA. 2008;14:2183-94 pubmed publisher
    ..Like the absence of the mcm(5) side chain, the lack of the s(2) group renders tRNA(mcm5s2UUC Glu) less sensitive to gamma-toxin, reinforcing the importance of the wobble nucleoside mcm(5)s(2)U for tRNA cleavage by gamma-toxin. ..
  9. Li Y, Takagi Y, Jiang Y, Tokunaga M, Erdjument Bromage H, Tempst P, et al. A multiprotein complex that interacts with RNA polymerase II elongator. J Biol Chem. 2001;276:29628-31 pubmed
    ..Deletions of genes for two Hap subunits, HAP1 and HAP3, confer pGKL killer-insensitive and weak Elongator phenotypes...

More Information

Publications17

  1. Winkler G, Kristjuhan A, Erdjument Bromage H, Tempst P, Svejstrup J. Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo. Proc Natl Acad Sci U S A. 2002;99:3517-22 pubmed
    ..The three smallest Elongator subunits--Elp4, Elp5, and Elp6--are required for HAT activity, and Elongator binds to both naked and nucleosomal DNA...
  2. Petrakis T, Wittschieben B, Svejstrup J. Molecular architecture, structure-function relationship, and importance of the Elp3 subunit for the RNA binding of holo-elongator. J Biol Chem. 2004;279:32087-92 pubmed
    ..In contrast, Elongator-RNA interaction requires the Elp3 protein. Together, these data shed light on the structure-function relationship of the Elongator complex. ..
  3. Xue Y, Kowalska A, Grabowska K, Przybyt K, Cichewicz M, Del Rosario B, et al. Histone chaperones Nap1 and Vps75 regulate histone acetylation during transcription elongation. Mol Cell Biol. 2013;33:1645-56 pubmed publisher
    ..This work sheds further light on the importance of histone chaperones as general regulators of transcription elongation. ..
  4. Setiaputra D, Cheng D, Lu S, Hansen J, Dalwadi U, Lam C, et al. Molecular architecture of the yeast Elongator complex reveals an unexpected asymmetric subunit arrangement. EMBO Rep. 2017;18:280-291 pubmed publisher
    ..Conserved from yeast to humans, Elongator is assembled from two copies of six unique subunits (Elp1 to Elp6)...
  5. Zabel R, Bär C, Mehlgarten C, Schaffrath R. Yeast alpha-tubulin suppressor Ats1/Kti13 relates to the Elongator complex and interacts with Elongator partner protein Kti11. Mol Microbiol. 2008;69:175-87 pubmed publisher
    ..In sum, our data suggest that Kti13 and Kti11 support Elongator functions and that they both share Elongator-independent role(s) that are important for cell viability. ..
  6. Greenwood C, Selth L, Dirac Svejstrup A, Svejstrup J. An iron-sulfur cluster domain in Elp3 important for the structural integrity of elongator. J Biol Chem. 2009;284:141-9 pubmed publisher
    ..Together our data support the idea that the Elp3 FeS cluster is essential for normal Elongator function in vivo primarily as a structural, rather than catalytic, domain. ..
  7. Laribee R, Krogan N, Xiao T, Shibata Y, Hughes T, Greenblatt J, et al. BUR kinase selectively regulates H3 K4 trimethylation and H2B ubiquitylation through recruitment of the PAF elongation complex. Curr Biol. 2005;15:1487-93 pubmed
    ..Our data reveal a novel function for the BUR kinase in transcriptional regulation through the selective control of histone modifications. ..
  8. Krogan N, Greenblatt J. Characterization of a six-subunit holo-elongator complex required for the regulated expression of a group of genes in Saccharomyces cerevisiae. Mol Cell Biol. 2001;21:8203-12 pubmed
    ..yeast Holo-Elongator complex containing three additional polypeptides, which we have named Elp4, Elp5, and Elp6. TAP tapping and subsequent purification of any one of the six subunits result in the isolation of all six ..