ELP4

Summary

Gene Symbol: ELP4
Description: Elongator subunit ELP4
Alias: HAP1, KTI9, TOT7, Elongator subunit ELP4
Species: Saccharomyces cerevisiae S288c
Products:     ELP4

Top Publications

  1. Lin Z, Zhao W, Diao W, Xie X, Wang Z, Zhang J, et al. Crystal structure of elongator subcomplex Elp4-6. J Biol Chem. 2012;287:21501-8 pubmed publisher
    Elongator is a multiprotein complex composed of two subcomplexes, Elp1-3 and Elp4-6...
  2. Krogan N, Greenblatt J. Characterization of a six-subunit holo-elongator complex required for the regulated expression of a group of genes in Saccharomyces cerevisiae. Mol Cell Biol. 2001;21:8203-12 pubmed
    ..purified a six-subunit yeast Holo-Elongator complex containing three additional polypeptides, which we have named Elp4, Elp5, and Elp6...
  3. Glatt S, Létoquart J, Faux C, Taylor N, Seraphin B, Müller C. The Elongator subcomplex Elp456 is a hexameric RecA-like ATPase. Nat Struct Mol Biol. 2012;19:314-20 pubmed publisher
    ..Our results support a role of Elongator in tRNA modification, explain the importance of each of the Elp4, Elp5 and Elp6 subunits for complex integrity and suggest a model for the overall architecture of the holo-..
  4. Greenwood C, Selth L, Dirac Svejstrup A, Svejstrup J. An iron-sulfur cluster domain in Elp3 important for the structural integrity of elongator. J Biol Chem. 2009;284:141-9 pubmed publisher
    ..Together our data support the idea that the Elp3 FeS cluster is essential for normal Elongator function in vivo primarily as a structural, rather than catalytic, domain. ..
  5. Winkler G, Petrakis T, Ethelberg S, Tokunaga M, Erdjument Bromage H, Tempst P, et al. RNA polymerase II elongator holoenzyme is composed of two discrete subcomplexes. J Biol Chem. 2001;276:32743-9 pubmed
    ..the previously identified Elp1, Elp2, and Elp3 proteins and another comprised of three novel polypeptides, termed Elp4, Elp5, and Elp6...
  6. Winkler G, Kristjuhan A, Erdjument Bromage H, Tempst P, Svejstrup J. Elongator is a histone H3 and H4 acetyltransferase important for normal histone acetylation levels in vivo. Proc Natl Acad Sci U S A. 2002;99:3517-22 pubmed
    ..The three smallest Elongator subunits--Elp4, Elp5, and Elp6--are required for HAT activity, and Elongator binds to both naked and nucleosomal DNA...
  7. Zabel R, Bär C, Mehlgarten C, Schaffrath R. Yeast alpha-tubulin suppressor Ats1/Kti13 relates to the Elongator complex and interacts with Elongator partner protein Kti11. Mol Microbiol. 2008;69:175-87 pubmed publisher
    ..In sum, our data suggest that Kti13 and Kti11 support Elongator functions and that they both share Elongator-independent role(s) that are important for cell viability. ..
  8. Parrish W, Stefan C, Emr S. PtdIns(3)P accumulation in triple lipid-phosphatase-deletion mutants triggers lethal hyperactivation of the Rho1p/Pkc1p cell-integrity MAP kinase pathway. J Cell Sci. 2005;118:5589-601 pubmed
    ..Taken together, the results of this study indicated that aberrant Rho1p/Pkc1p signaling contributes to the lethal effects of PtdIns(3)P accumulation in cells deficient in PI 3-phosphatase activity. ..
  9. Huang B, Lu J, Byström A. A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. RNA. 2008;14:2183-94 pubmed publisher
    ..Like the absence of the mcm(5) side chain, the lack of the s(2) group renders tRNA(mcm5s2UUC Glu) less sensitive to gamma-toxin, reinforcing the importance of the wobble nucleoside mcm(5)s(2)U for tRNA cleavage by gamma-toxin. ..

More Information

Publications20

  1. Li Y, Takagi Y, Jiang Y, Tokunaga M, Erdjument Bromage H, Tempst P, et al. A multiprotein complex that interacts with RNA polymerase II elongator. J Biol Chem. 2001;276:29628-31 pubmed
    ..Deletions of genes for two Hap subunits, HAP1 and HAP3, confer pGKL killer-insensitive and weak Elongator phenotypes...
  2. Xue Y, Kowalska A, Grabowska K, Przybyt K, Cichewicz M, Del Rosario B, et al. Histone chaperones Nap1 and Vps75 regulate histone acetylation during transcription elongation. Mol Cell Biol. 2013;33:1645-56 pubmed publisher
    ..This work sheds further light on the importance of histone chaperones as general regulators of transcription elongation. ..
  3. Xu H, Bygdell J, Wingsle G, Byström A. Yeast Elongator protein Elp1p does not undergo proteolytic processing in exponentially growing cells. Microbiologyopen. 2015;4:867-78 pubmed publisher
    ..Consequently, our results indicate that N-terminal truncation of Elp1p is not likely to regulate Elongator complex activity. ..
  4. Daudén M, Kosinski J, Kolaj Robin O, Desfosses A, Ori A, Faux C, et al. Architecture of the yeast Elongator complex. EMBO Rep. 2017;18:264-279 pubmed publisher
    ..Our study provides a structural framework on how the tRNA modification activity is carried out by Elongator. ..
  5. Petrakis T, Søgaard T, Erdjument Bromage H, Tempst P, Svejstrup J. Physical and functional interaction between Elongator and the chromatin-associated Kti12 protein. J Biol Chem. 2005;280:19454-60 pubmed
    ..Together, these experiments indicate a close physical and functional relationship between Elongator and the highly conserved Kti12 protein. ..
  6. Petrakis T, Wittschieben B, Svejstrup J. Molecular architecture, structure-function relationship, and importance of the Elp3 subunit for the RNA binding of holo-elongator. J Biol Chem. 2004;279:32087-92 pubmed
    ..Elp2 and Elp4 were largely dispensable for the association of Elongator with nascent RNA transcript in vivo...
  7. James A, Cocheme H, Murai M, Miyoshi H, Murphy M. Complementation of coenzyme Q-deficient yeast by coenzyme Q analogues requires the isoprenoid side chain. FEBS J. 2010;277:2067-82 pubmed publisher
    ..Here we suggest that CoQ or its redox state may be a signal for growth during the shift to respiration. ..
  8. Chen C, Huang B, Eliasson M, Ryden P, Byström A. Elongator complex influences telomeric gene silencing and DNA damage response by its role in wobble uridine tRNA modification. PLoS Genet. 2011;7:e1002258 pubmed publisher
    ..Consistent with this notion, we found that expression of Sir4, a silent information regulator required for assembly of silent chromatin at telomeres, was decreased in the elp3? mutants. ..
  9. Laribee R, Krogan N, Xiao T, Shibata Y, Hughes T, Greenblatt J, et al. BUR kinase selectively regulates H3 K4 trimethylation and H2B ubiquitylation through recruitment of the PAF elongation complex. Curr Biol. 2005;15:1487-93 pubmed
    ..Our data reveal a novel function for the BUR kinase in transcriptional regulation through the selective control of histone modifications. ..
  10. Xu H, Lin Z, Li F, Diao W, Dong C, Zhou H, et al. Dimerization of elongator protein 1 is essential for Elongator complex assembly. Proc Natl Acad Sci U S A. 2015;112:10697-702 pubmed publisher
  11. Setiaputra D, Cheng D, Lu S, Hansen J, Dalwadi U, Lam C, et al. Molecular architecture of the yeast Elongator complex reveals an unexpected asymmetric subunit arrangement. EMBO Rep. 2017;18:280-291 pubmed publisher
    ..This work provides the first structural insights into Elongator and a framework to understand the molecular basis of its multifunctionality. ..