ELO2

Summary

Gene Symbol: ELO2
Description: fatty acid elongase ELO2
Alias: FEN1, GNS1, VBM2, fatty acid elongase ELO2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Silve S, Leplatois P, Josse A, Dupuy P, Lanau C, Kaghad M, et al. The immunosuppressant SR 31747 blocks cell proliferation by inhibiting a steroid isomerase in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:2719-27 pubmed
    ..cells growing in the absence of exogenous ergosterol, except in SR-resistant mutants lacking either the SUR4 or the FEN1 gene product...
  2. David D, Sundarababu S, Gerst J. Involvement of long chain fatty acid elongation in the trafficking of secretory vesicles in yeast. J Cell Biol. 1998;143:1167-82 pubmed
    ..Interestingly, VBM1 and VBM2 are allelic to ELO3 and ELO2, two genes that have been shown recently to mediate the elongation of very long chain fatty acids and subsequent ..
  3. Budd M, Campbell J. A yeast replicative helicase, Dna2 helicase, interacts with yeast FEN-1 nuclease in carrying out its essential function. Mol Cell Biol. 1997;17:2136-42 pubmed
    ..The simplest interpretation of these data is that one of the roles of Dna2 helicase is associated with processing of Okazaki fragments. ..
  4. Oh C, Toke D, Mandala S, Martin C. ELO2 and ELO3, homologues of the Saccharomyces cerevisiae ELO1 gene, function in fatty acid elongation and are required for sphingolipid formation. J Biol Chem. 1997;272:17376-84 pubmed
    b>ELO2 and ELO3 were identified from the Saccharomyces cerevisiae genome data base as homologues of ELO1, a gene involved in the elongation of the fatty acid 14:0 to 16:0...
  5. Revardel E, Bonneau M, Durrens P, Aigle M. Characterization of a new gene family developing pleiotropic phenotypes upon mutation in Saccharomyces cerevisiae. Biochim Biophys Acta. 1995;1263:261-5 pubmed
    ..Sequence comparison revealed that two yeast genes, FEN1 and JO343, shared significant similarities with SUR4...
  6. Hodge C, Choudhary V, Wolyniak M, Scarcelli J, Schneiter R, Cole C. Integral membrane proteins Brr6 and Apq12 link assembly of the nuclear pore complex to lipid homeostasis in the endoplasmic reticulum. J Cell Sci. 2010;123:141-51 pubmed publisher
    ..These data indicate that Brr6 has an essential function in regulating lipid homeostasis in the NE-ER, thereby impacting NPC formation and nucleocytoplasmic transport. ..
  7. Yang J, Freudenreich C. Haploinsufficiency of yeast FEN1 causes instability of expanded CAG/CTG tracts in a length-dependent manner. Gene. 2007;393:110-5 pubmed
    ..Flap endonuclease 1 (Fen1), has an endonuclease activity specific for 5' flap structures and is involved in Okazaki fragment processing and ..
  8. Tabuchi M, Audhya A, Parsons A, Boone C, Emr S. The phosphatidylinositol 4,5-biphosphate and TORC2 binding proteins Slm1 and Slm2 function in sphingolipid regulation. Mol Cell Biol. 2006;26:5861-75 pubmed
    ..Together, our data suggest that Slm1 and Slm2 define a molecular link between phosphoinositide and sphingolipid signaling and thereby regulate actin cytoskeleton organization. ..
  9. Borges V, Smith D, Whitehouse I, Uhlmann F. An Eco1-independent sister chromatid cohesion establishment pathway in S. cerevisiae. Chromosoma. 2013;122:121-34 pubmed publisher
    ..Thus, Ctf4 and Chl1 delineate an additional acetylation-independent pathway that might hold important clues as to the mechanism of sister chromatid cohesion establishment...

More Information

Publications27

  1. Olson D, Fröhlich F, Christiano R, Hannibal Bach H, Ejsing C, Walther T. Rom2-dependent phosphorylation of Elo2 controls the abundance of very long-chain fatty acids. J Biol Chem. 2015;290:4238-47 pubmed publisher
    ..Here we show that Elo2, a key enzyme of VLCFA synthesis, is controlled by signaling of the guanine nucleotide exchange factor Rom2, ..
  2. Ragni E, Piberger H, Neupert C, García Cantalejo J, Popolo L, Arroyo J, et al. The genetic interaction network of CCW12, a Saccharomyces cerevisiae gene required for cell wall integrity during budding and formation of mating projections. BMC Genomics. 2011;12:107 pubmed publisher
    ..Among those are PFD1, WHI3, SRN2, PAC10, FEN1 and YDR417C, which have not been related to cell wall integrity before...
  3. Balguerie A, Bagnat M, Bonneu M, Aigle M, Breton A. Rvs161p and sphingolipids are required for actin repolarization following salt stress. Eukaryot Cell. 2002;1:1021-31 pubmed
    ..suppressing the rvs161delta-related salt sensitivity all occurred in genes required for sphingolipid biosynthesis: FEN1, SUR4, SUR2, SUR1, and IPT1...
  4. Kohlwein S, Eder S, Oh C, Martin C, Gable K, Bacikova D, et al. Tsc13p is required for fatty acid elongation and localizes to a novel structure at the nuclear-vacuolar interface in Saccharomyces cerevisiae. Mol Cell Biol. 2001;21:109-25 pubmed
    ..These phenotypes are exacerbated by the deletion of either the ELO2 or ELO3 gene, both of which have previously been shown to be required for VLCFA synthesis...
  5. Daquinag A, Fadri M, Jung S, Qin J, Kunz J. The yeast PH domain proteins Slm1 and Slm2 are targets of sphingolipid signaling during the response to heat stress. Mol Cell Biol. 2007;27:633-50 pubmed
  6. Singh P, Zheng L, Chavez V, Qiu J, Shen B. Concerted action of exonuclease and Gap-dependent endonuclease activities of FEN-1 contributes to the resolution of triplet repeat sequences (CTG)n- and (GAA)n-derived secondary structures formed during maturation of Okazaki fragments. J Biol Chem. 2007;282:3465-77 pubmed
    ..However, the absence of Fen1 in yeast results in a significant increase in trinucleotide repeat (TNR) expansion...
  7. Gomes X, Burgers P. Two modes of FEN1 binding to PCNA regulated by DNA. EMBO J. 2000;19:3811-21 pubmed
    The FEN1 nuclease functions during Okazaki fragment maturation in the eukaryotic cell...
  8. Han G, Gable K, Kohlwein S, Beaudoin F, Napier J, Dunn T. The Saccharomyces cerevisiae YBR159w gene encodes the 3-ketoreductase of the microsomal fatty acid elongase. J Biol Chem. 2002;277:35440-9 pubmed
    ..By disrupting the orthologs of Ybr159w in the ybr159Delta mutant we found that the ybr159Deltaayr1Delta double mutant was inviable, suggesting that Ayr1p is responsible for the residual 3-ketoreductase activity. ..
  9. Tseng H, Tomkinson A. Processing and joining of DNA ends coordinated by interactions among Dnl4/Lif1, Pol4, and FEN-1. J Biol Chem. 2004;279:47580-8 pubmed
  10. Obara K, Kojima R, Kihara A. Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids. J Lipid Res. 2013;54:831-42 pubmed publisher
    ..These results suggest that, of all the intracellular trafficking pathways, requirement of VLCFAs is especially high in the endosomal pathways. ..
  11. Tvrdik P, Westerberg R, Silve S, Asadi A, Jakobsson A, Cannon B, et al. Role of a new mammalian gene family in the biosynthesis of very long chain fatty acids and sphingolipids. J Cell Biol. 2000;149:707-18 pubmed
    ..Similarly, Cig30 reverted the phenotype of the fen1/elo2 mutant that has reduced levels of fatty acids in the C(20)-C(24) range...
  12. Choi J, Martin C. The Saccharomyces cerevisiae FAT1 gene encodes an acyl-CoA synthetase that is required for maintenance of very long chain fatty acid levels. J Biol Chem. 1999;274:4671-83 pubmed
    ..Simultaneous disruption of FAA1 and FAA4, which encode long chain (C14-C18) fatty acyl-CoA synthetases, effectively blocks the import of long chain saturated and unsaturated fatty acids. ..
  13. Jain S, Stanford N, Bhagwat N, Seiler B, Costanzo M, Boone C, et al. Identification of a novel lysophospholipid acyltransferase in Saccharomyces cerevisiae. J Biol Chem. 2007;282:30562-9 pubmed
    ..Therefore, Lpt1p, a member of the membrane-bound o-acyltransferase gene family, seems to work in conjunction with Slc1 to mediate the incorporation of unsaturated acyl chains into the sn-2 position of phospholipids. ..
  14. Rossler H, Rieck C, Delong T, Hoja U, Schweizer E. Functional differentiation and selective inactivation of multiple Saccharomyces cerevisiae genes involved in very-long-chain fatty acid synthesis. Mol Genet Genomics. 2003;269:290-8 pubmed
    ..Elongases I, II and III are specifically inactivated in, respectively, elo1, elo2 and elo3 mutants. Elongases II and III share the same 3-ketoacyl reductase, which is encoded by the YBR159w gene...
  15. Lone M, Atkinson A, Hodge C, Cottier S, Martínez Montañés F, Maithel S, et al. Yeast Integral Membrane Proteins Apq12, Brl1, and Brr6 Form a Complex Important for Regulation of Membrane Homeostasis and Nuclear Pore Complex Biogenesis. Eukaryot Cell. 2015;14:1217-27 pubmed publisher
    ..We suggest that the defects in nuclear pore complex biogenesis and mRNA export seen in these mutants are consequences of defects in maintaining the biophysical properties of the NE. ..
  16. Bailly Bechet M, Borgs C, Braunstein A, Chayes J, Dagkessamanskaia A, Francois J, et al. Finding undetected protein associations in cell signaling by belief propagation. Proc Natl Acad Sci U S A. 2011;108:882-7 pubmed publisher
    ..The algorithm we present is specially suited for very large datasets, can run in parallel, and can be adapted to other problems in systems biology. On renowned benchmarks it outperforms other algorithms in the field. ..
  17. Copic A, Latham C, Horlbeck M, D Arcangelo J, Miller E. ER cargo properties specify a requirement for COPII coat rigidity mediated by Sec13p. Science. 2012;335:1359-62 pubmed publisher
    ..Thus, Sec13p may rigidify the COPII cage and increase its membrane-bending capacity; this function could be bypassed when a bst mutation renders the membrane more deformable. ..
  18. Zimmermann C, Santos A, Gable K, Epstein S, Gururaj C, Chymkowitch P, et al. TORC1 inhibits GSK3-mediated Elo2 phosphorylation to regulate very long chain fatty acid synthesis and autophagy. Cell Rep. 2013;5:1036-46 pubmed publisher
    ..study was to identify mechanisms that regulate the rate of VLCFA synthesis, and we discovered that the fatty acid elongase Elo2 is regulated by phosphorylation...