EGD2

Summary

Gene Symbol: EGD2
Description: Egd2p
Alias: Egd2p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Reimann B, Bradsher J, Franke J, Hartmann E, Wiedmann M, Prehn S, et al. Initial characterization of the nascent polypeptide-associated complex in yeast. Yeast. 1999;15:397-407 pubmed
    ..polypeptide-associated complex (alpha, beta1, beta3) in Saccharomyces cerevisiae are encoded by three genes (EGD2, EGD1, BTT1)...
  2. Franke J, Reimann B, Hartmann E, Köhlerl M, Wiedmann B. Evidence for a nuclear passage of nascent polypeptide-associated complex subunits in yeast. J Cell Sci. 2001;114:2641-8 pubmed
    ..We hypothesize that newly translated NAC proteins travel into the nucleus to bind stoichiometrically to ribosomal subunits and then leave the nucleus together with these subunits to concentrate in the cytosol. ..
  3. Panasenko O, David F, Collart M. Ribosome association and stability of the nascent polypeptide-associated complex is dependent upon its own ubiquitination. Genetics. 2009;181:447-60 pubmed publisher
    ..While simultaneous mutation of many lysines in the alpha-subunit of NAC (Egd2p) was required to abolish its ubiquitination, for the beta-subunit of NAC (Egd1p), mutation of K29 and K30 was ..
  4. Wiedmann B, Prehn S. The nascent polypeptide-associated complex (NAC) of yeast functions in the targeting process of ribosomes to the ER membrane. FEBS Lett. 1999;458:51-4 pubmed
    ..In the absence of NAC, signal-less RNCs are able to bind to ER membranes. However, following puromycin treatment, only very few nascent chains translocate into the lumen, as detected by glycosylation. ..
  5. George R, Beddoe T, Landl K, Lithgow T. The yeast nascent polypeptide-associated complex initiates protein targeting to mitochondria in vivo. Proc Natl Acad Sci U S A. 1998;95:2296-301 pubmed
    The yeast nascent polypeptide-associated complex (NAC) is encoded by two genes, EGD1 and EGD2, and is associated with cytoplasmic ribosomes...
  6. Panasenko O, Landrieux E, Feuermann M, Finka A, Paquet N, Collart M. The yeast Ccr4-Not complex controls ubiquitination of the nascent-associated polypeptide (NAC-EGD) complex. J Biol Chem. 2006;281:31389-98 pubmed
    ..ribosome-associated heterodimeric EGD (enhancer of Gal4p DNA binding) complex, which consists of the Egd1p and Egd2p subunits in yeast and is named NAC (nascent polypeptide-associated complex) in mammals...
  7. Pech M, Spreter T, Beckmann R, Beatrix B. Dual binding mode of the nascent polypeptide-associated complex reveals a novel universal adapter site on the ribosome. J Biol Chem. 2010;285:19679-87 pubmed publisher
    ..In contrast, alphaNAC (Egd2p) contacts Rpl17, the direct neighbor of Rpl31 at the ribosomal tunnel exit site...
  8. Koplin A, Preissler S, Ilina Y, Koch M, Scior A, Erhardt M, et al. A dual function for chaperones SSB-RAC and the NAC nascent polypeptide-associated complex on ribosomes. J Cell Biol. 2010;189:57-68 pubmed publisher
    ..These findings emphasize the concept that ribosome production is coordinated with the protein-folding capacity of ribosome-associated chaperones. ..
  9. del Alamo M, Hogan D, Pechmann S, Albanèse V, Brown P, Frydman J. Defining the specificity of cotranslationally acting chaperones by systematic analysis of mRNAs associated with ribosome-nascent chain complexes. PLoS Biol. 2011;9:e1001100 pubmed publisher
    ..The strategy we used should be generally applicable to mapping the specificity, interplay, and dynamics of the cotranslational protein homeostasis network. ..

More Information

Publications17

  1. Keefer K, True H. Prion-Associated Toxicity is Rescued by Elimination of Cotranslational Chaperones. PLoS Genet. 2016;12:e1006431 pubmed publisher
    ..This toxicity rescue demonstrates that chaperone modification can block key steps of the prion life cycle and has exciting implications for potential treatment of many human protein conformational disorders. ..
  2. Möckli N, Deplazes A, Hassa P, Zhang Z, Peter M, Hottiger M, et al. Yeast split-ubiquitin-based cytosolic screening system to detect interactions between transcriptionally active proteins. Biotechniques. 2007;42:725-30 pubmed
    ..The cytoY2H system extends existing methods for the detection of protein interactions by providing a convenient solution for screening a wide range of transcriptionally active proteins. ..
  3. Fernández Murray J, McMaster C. Identification of novel phospholipid binding proteins in Saccharomyces cerevisiae. FEBS Lett. 2006;580:82-6 pubmed
    ..This simple approach could be systematically applied to perform an exhaustive screen for soluble lipid binding proteins in S. cerevisiae or other organisms. ..
  4. Takahashi T, Hirose K, Mizutani E, Naganuma A. Dysfunctional nascent polypeptide-associated complex (NAC) activity in ribosomes enhances adriamycin toxicity in budding yeast. J Toxicol Sci. 2009;34:703-8 pubmed
    ..This revealed that adriamycin toxicity was enhanced byloss of the Egd1 or Egd2 subunits of the nascent polypeptide-associated complex(NAC)...
  5. Hiraishi H, Shimada T, Ohtsu I, Sato T, Takagi H. The yeast ubiquitin ligase Rsp5 downregulates the alpha subunit of nascent polypeptide-associated complex Egd2 under stress conditions. FEBS J. 2009;276:5287-97 pubmed publisher
    ..obtained indicate that several proteins, including the a-subunit of nascent polypeptide-associated complex (aNAC, Egd2) accumulated in the rsp5 mutant...
  6. Zhang Y, Berndt U, Gölz H, Tais A, Oellerer S, Wölfle T, et al. NAC functions as a modulator of SRP during the early steps of protein targeting to the endoplasmic reticulum. Mol Biol Cell. 2012;23:3027-40 pubmed publisher
    ..The combined data indicate a functional interplay between NAC and SRP on ER-targeted RNCs, which is based on the ability of the two complexes to bind simultaneously to distinct segments of a single nascent chain. ..
  7. Lesnik C, Cohen Y, Atir Lande A, Schuldiner M, Arava Y. OM14 is a mitochondrial receptor for cytosolic ribosomes that supports co-translational import into mitochondria. Nat Commun. 2014;5:5711 pubmed publisher
    ..These results provide a strong support for an additional, co-translational mode of import into mitochondria. ..
  8. Nyathi Y, Pool M. Analysis of the interplay of protein biogenesis factors at the ribosome exit site reveals new role for NAC. J Cell Biol. 2015;210:287-301 pubmed publisher
    ..NAC also functions in SRP-dependent targeting and helps to protect substrates from aggregation before translocation. ..