DUN1

Summary

Gene Symbol: DUN1
Description: serine/threonine protein kinase DUN1
Alias: serine/threonine protein kinase DUN1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Kats E, Albuquerque C, Zhou H, Kolodner R. Checkpoint functions are required for normal S-phase progression in Saccharomyces cerevisiae RCAF- and CAF-I-defective mutants. Proc Natl Acad Sci U S A. 2006;103:3710-5 pubmed
    ..Furthermore, asf1 mutants had high levels of Ddc2.GFP foci that were further increased in asf1 dun1 double mutants consistent with a requirement for checkpoint proteins in S-phase progression in asf1 mutants, ..
  2. Zhao X, Muller E, Rothstein R. A suppressor of two essential checkpoint genes identifies a novel protein that negatively affects dNTP pools. Mol Cell. 1998;2:329-40 pubmed
    ..Finally, both in vivo and in vitro experiments show that Sml1 binds to Rnr1. We propose that Sml1 inhibits dNTP synthesis posttranslationally by binding directly to Rnr1 and that Mec1 and Rad53 are required to relieve this inhibition. ..
  3. Fasullo M, Koudelik J, AhChing P, Giallanza P, Cera C. Radiosensitive and mitotic recombination phenotypes of the Saccharomyces cerevisiae dun1 mutant defective in DNA damage-inducible gene expression. Genetics. 1999;152:909-19 pubmed
    ..We investigated the role of DUN1-mediated, DNA damage-inducible gene expression in conferring radiation resistance in Saccharomyces cerevisiae...
  4. Huang M, Elledge S. Identification of RNR4, encoding a second essential small subunit of ribonucleotide reductase in Saccharomyces cerevisiae. Mol Cell Biol. 1997;17:6105-13 pubmed
    ..RNR4 is inducible by DNA-damaging agents through the same signal transduction pathway involving MEC1, RAD53, and DUN1 kinase genes...
  5. Schollaert K, Poisson J, Searle J, Schwanekamp J, Tomlinson C, Sanchez Y. A role for Saccharomyces cerevisiae Chk1p in the response to replication blocks. Mol Biol Cell. 2004;15:4051-63 pubmed
    ..cerevisiae and suggest that at least in some genetic backgrounds, the Chk1p/securin pathway is required for the recovery from stalled or collapsed replication forks. ..
  6. Uchiki T, Dice L, Hettich R, Dealwis C. Identification of phosphorylation sites on the yeast ribonucleotide reductase inhibitor Sml1. J Biol Chem. 2004;279:11293-303 pubmed
    ..Sml1 is a downstream effector of the Mec1/Rad53 cell cycle checkpoint pathway. The phosphorylation by Dun1 kinase during S phase or in response to DNA damage leads to diminished levels of Sml1...
  7. Fasullo M, Tsaponina O, Sun M, Chabes A. Elevated dNTP levels suppress hyper-recombination in Saccharomyces cerevisiae S-phase checkpoint mutants. Nucleic Acids Res. 2010;38:1195-203 pubmed publisher
    ..By introducing a dun1 mutation, thus eliminating inducible expression of ribonucleotide reductase in mec1-21, rates of spontaneous SCE ..
  8. Myung K, Chen C, Kolodner R. Multiple pathways cooperate in the suppression of genome instability in Saccharomyces cerevisiae. Nature. 2001;411:1073-6 pubmed
    ..Mutations that inactivate these pathways cause high rates of GCRs and show synergistic interactions, indicating that the pathways that suppress GCRs all compete for the same DNA substrates. ..
  9. Huang M, Zhou Z, Elledge S. The DNA replication and damage checkpoint pathways induce transcription by inhibition of the Crt1 repressor. Cell. 1998;94:595-605 pubmed
    ..The inhibition of an autoregulatory repressor in response to DNA damage is a strategy conserved throughout prokaryotic and eukaryotic evolution. ..

More Information

Publications61

  1. Lee Y, Wang J, Stubbe J, Elledge S. Dif1 is a DNA-damage-regulated facilitator of nuclear import for ribonucleotide reductase. Mol Cell. 2008;32:70-80 pubmed publisher
    ..Dif1 is both cell-cycle and DNA-damage regulated, the latter of which occurs via the Mec1-Dun1 pathway...
  2. Kumar D, Viberg J, Nilsson A, Chabes A. Highly mutagenic and severely imbalanced dNTP pools can escape detection by the S-phase checkpoint. Nucleic Acids Res. 2010;38:3975-83 pubmed publisher
    ..In contrast, when none of the dNTPs was limiting for DNA replication, even extreme and mutagenic dNTP pool imbalances did not activate the S-phase checkpoint and did not interfere with the cell cycle progression. ..
  3. Zhao X, Rothstein R. The Dun1 checkpoint kinase phosphorylates and regulates the ribonucleotide reductase inhibitor Sml1. Proc Natl Acad Sci U S A. 2002;99:3746-51 pubmed
    ..Here we show that Dun1, a downstream kinase of Mec1/Rad53, genetically and physically interacts with Sml1 in vivo...
  4. Hu F, Alcasabas A, Elledge S. Asf1 links Rad53 to control of chromatin assembly. Genes Dev. 2001;15:1061-6 pubmed
    ..Thus, checkpoint pathways directly regulate chromatin assembly to promote survival in response to DNA damage and replication blocks. ..
  5. Zhao X, Georgieva B, Chabes A, Domkin V, Ippel J, Schleucher J, et al. Mutational and structural analyses of the ribonucleotide reductase inhibitor Sml1 define its Rnr1 interaction domain whose inactivation allows suppression of mec1 and rad53 lethality. Mol Cell Biol. 2000;20:9076-83 pubmed
    ..Finally, the seven sml1 mutations also disrupt the interaction with yeast Rnr3 and human R1, suggesting a conserved binding mechanism between Sml1 and the large subunit of RNR from different species. ..
  6. Gardner R, Putnam C, Weinert T. RAD53, DUN1 and PDS1 define two parallel G2/M checkpoint pathways in budding yeast. EMBO J. 1999;18:3173-85 pubmed
    ..g. RAD9 and RAD24), those transducing a signal (MEC1, RAD53 and DUN1) or those participating more directly in arrest (PDS1)...
  7. Tsaponina O, Barsoum E, Aström S, Chabes A. Ixr1 is required for the expression of the ribonucleotide reductase Rnr1 and maintenance of dNTP pools. PLoS Genet. 2011;7:e1002061 pubmed publisher
    The Saccharomyces cerevisiae Dun1 protein kinase is a downstream target of the conserved Mec1-Rad53 checkpoint pathway...
  8. Chen S, Smolka M, Zhou H. Mechanism of Dun1 activation by Rad53 phosphorylation in Saccharomyces cerevisiae. J Biol Chem. 2007;282:986-95 pubmed
    ..To better dissect this mechanism, we developed an activity-based assay for Dun1, a downstream DNA damage check-point kinase in yeast, using its physiological substrate Sml1...
  9. Bastos de Oliveira F, Harris M, Brazauskas P, de Bruin R, Smolka M. Linking DNA replication checkpoint to MBF cell-cycle transcription reveals a distinct class of G1/S genes. EMBO J. 2012;31:1798-810 pubmed publisher
    ..Our results reveal that replication stress induces expression of G1/S genes via the Rad53-MBF pathway and that an SBF-to-MBF switch characterizes a new class of genes that can be induced by replication stress. ..
  10. Bashkirov V, Bashkirova E, Haghnazari E, Heyer W. Direct kinase-to-kinase signaling mediated by the FHA phosphoprotein recognition domain of the Dun1 DNA damage checkpoint kinase. Mol Cell Biol. 2003;23:1441-52 pubmed
    The serine-threonine kinase Dun1 contains a forkhead-associated (FHA) domain and functions in the DNA damage checkpoint pathway of Saccharomyces cerevisiae. It belongs to the Chk2 family of checkpoint kinases, which includes S...
  11. Lin C, Wu M, Gay S, Marjavaara L, Lai M, Hsiao W, et al. H2B mono-ubiquitylation facilitates fork stalling and recovery during replication stress by coordinating Rad53 activation and chromatin assembly. PLoS Genet. 2014;10:e1004667 pubmed publisher
  12. Hwang J, Smith S, Ceschia A, Torres Rosell J, Aragon L, Myung K. Smc5-Smc6 complex suppresses gross chromosomal rearrangements mediated by break-induced replications. DNA Repair (Amst). 2008;7:1426-36 pubmed publisher
  13. Tsang C, Liu Y, Thomas J, Zhang Y, Zheng X. Superoxide dismutase 1 acts as a nuclear transcription factor to regulate oxidative stress resistance. Nat Commun. 2014;5:3446 pubmed publisher
    ..ROS signalling is mediated by Mec1/ATM and its effector Dun1/Cds1 kinase, through Dun1 interaction with Sod1 and regulation of Sod1 by phosphorylation at S60, 99...
  14. Kats E, Enserink J, Martinez S, Kolodner R. The Saccharomyces cerevisiae Rad6 postreplication repair and Siz1/Srs2 homologous recombination-inhibiting pathways process DNA damage that arises in asf1 mutants. Mol Cell Biol. 2009;29:5226-37 pubmed publisher
    ..seen in asf1 mutants, which was independent of translesion bypass polymerases but showed an increased dependency on Dun1. Combining an asf1 deletion with different PRR mutations resulted in a synergistic increase in sensitivity to ..
  15. Chen E, Weng J, Chen Y, Wang S, Liu X, Huang W, et al. Phospho-Priming Confers Functionally Relevant Specificities for Rad53 Kinase Autophosphorylation. Biochemistry. 2017;56:5112-5124 pubmed publisher
    ..Our results show that, while Rad53 can display active conformations under various conditions, simulation of in vivo regulatory conditions confers functionally relevant autophosphorylation. ..
  16. Hammet A, Pike B, Heierhorst J. Posttranscriptional regulation of the RAD5 DNA repair gene by the Dun1 kinase and the Pan2-Pan3 poly(A)-nuclease complex contributes to survival of replication blocks. J Biol Chem. 2002;277:22469-74 pubmed
    The yeast Dun1 kinase has complex checkpoint functions including DNA damage-dependent cell cycle arrest in G(2)/M, transcriptional induction of repair genes, and regulation of postreplicative DNA repair pathways...
  17. Shen J, Srivas R, Gross A, Li J, Jaehnig E, Sun S, et al. Chemogenetic profiling identifies RAD17 as synthetically lethal with checkpoint kinase inhibition. Oncotarget. 2015;6:35755-69 pubmed publisher
    ..Taken together, our data suggest that CHEK1 or WEE1 inhibitors are likely to have greater clinical efficacy in tumors with RAD17 loss-of-function. ..
  18. Herr A, Kennedy S, Knowels G, Schultz E, Preston B. DNA replication error-induced extinction of diploid yeast. Genetics. 2014;196:677-91 pubmed publisher
    ..Conversely, inactivation of the Dun1 S-phase checkpoint kinase suppresses strong pol3-01,L612M,eex and pol3-01,L612G,eex mutator phenotypes as well as ..
  19. Traven A, Hammet A, Tenis N, Denis C, Heierhorst J. Ccr4-not complex mRNA deadenylase activity contributes to DNA damage responses in Saccharomyces cerevisiae. Genetics. 2005;169:65-75 pubmed
    ..encode the major cytoplasmic mRNA deadenylase complex, have complex genetic interactions with the checkpoint genes DUN1, MRC1, RAD9, and RAD17 in response to DNA-damaging agents hydroxyurea (HU) and methylmethane sulfonate (MMS)...
  20. Myung K, Kolodner R. Suppression of genome instability by redundant S-phase checkpoint pathways in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2002;99:4500-7 pubmed
    ..These data support the view that spontaneous genome rearrangements result from DNA replication errors and indicate that there is a high degree of redundancy among the checkpoints that act in S phase to suppress such genome instability. ..
  21. Mertz T, Sharma S, Chabes A, Shcherbakova P. Colon cancer-associated mutator DNA polymerase δ variant causes expansion of dNTP pools increasing its own infidelity. Proc Natl Acad Sci U S A. 2015;112:E2467-76 pubmed publisher
  22. Marsolier M, Roussel P, Leroy C, Mann C. Involvement of the PP2C-like phosphatase Ptc2p in the DNA checkpoint pathways of Saccharomyces cerevisiae. Genetics. 2000;154:1523-32 pubmed
    ..overexpression induces hypersensitivity to genotoxic agents in wild-type cells and is lethal to rad53, mec1, and dun1 mutants with low ribonucleotide reductase activity...
  23. Yoshitani A, Ling F, Yoshida M. The yeast checkpoint kinase Dun1 downregulates DIN7 in the absence of DNA damage. Biosci Biotechnol Biochem. 2008;72:1630-4 pubmed
    Yeast DIN7 is a DNA damage-inducible gene. Its expression is increased in the absence of Dun1, a DNA damage checkpoint kinase...
  24. Hoch N, Chen E, Buckland R, Wang S, Fazio A, Hammet A, et al. Molecular basis of the essential s phase function of the rad53 checkpoint kinase. Mol Cell Biol. 2013;33:3202-13 pubmed publisher
    ..This phenotype is caused by an inability of rad53-4AQ to activate the downstream kinase Dun1, which then leads to reduced basal deoxynucleoside triphosphate (dNTP) levels, spontaneous replication fork ..
  25. Kaochar S, Shanks L, Weinert T. Checkpoint genes and Exo1 regulate nearby inverted repeat fusions that form dicentric chromosomes in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2010;107:21605-10 pubmed publisher
    ..whereas in a second pathway the ATR-like kinases Mec1 and Tel1, adaptor protein Rad9, and effector kinases Chk1 and Dun1 act independently of Exo1 to prevent inverted repeat fusion...
  26. Fikus M, Mieczkowski P, Koprowski P, Rytka J, Sledziewska Gojska E, Ciesla Z. The product of the DNA damage-inducible gene of Saccharomyces cerevisiae, DIN7, specifically functions in mitochondria. Genetics. 2000;154:73-81 pubmed
    ..Here, we report that Din7p specifically affects metabolism of mitochondrial DNA (mtDNA). We have found that dun1 strains, defective in the transcriptional activation of the DNA damage-inducible genes RNR1, RNR2, and RNR3, ..
  27. Sharp J, Rizki G, Kaufman P. Regulation of histone deposition proteins Asf1/Hir1 by multiple DNA damage checkpoint kinases in Saccharomyces cerevisiae. Genetics. 2005;171:885-99 pubmed
    ..Furthermore, deletion of the Dun1 kinase, a downstream target of Rad53, also suppressed the silencing defects of cac1Delta cells and reduced the ..
  28. Liang J, Suhandynata R, Zhou H. Phosphorylation of Sae2 Mediates Forkhead-associated (FHA) Domain-specific Interaction and Regulates Its DNA Repair Function. J Biol Chem. 2015;290:10751-63 pubmed publisher
    ..We found that phosphorylation of Thr-90 of Sae2 mediates its interaction with Rad53, Dun1, Xrs2, Dma1, and Dma2, whereas Rad53 and Dun1 additionally interact with phosphorylated Thr-279 of Sae2...
  29. Zhang Y, Li H, Zhang C, An X, Liu L, Stubbe J, et al. Conserved electron donor complex Dre2-Tah18 is required for ribonucleotide reductase metallocofactor assembly and DNA synthesis. Proc Natl Acad Sci U S A. 2014;111:E1695-704 pubmed publisher
    ..Together, these findings provide insight into RNR cofactor formation and reveal a shared mechanism underlying assembly of the Fe(III)2-Y• cofactor in RNR and the Fe-S clusters in cytosolic and nuclear proteins. ..
  30. Andreson B, Gupta A, Georgieva B, Rothstein R. The ribonucleotide reductase inhibitor, Sml1, is sequentially phosphorylated, ubiquitylated and degraded in response to DNA damage. Nucleic Acids Res. 2010;38:6490-501 pubmed publisher
    ..The Mec1/Rad53/Dun1 DNA damage response kinase cascade exhibits multifaceted controls over RNR activity including the regulation of the ..
  31. Woolstencroft R, Beilharz T, Cook M, Preiss T, Durocher D, Tyers M. Ccr4 contributes to tolerance of replication stress through control of CRT1 mRNA poly(A) tail length. J Cell Sci. 2006;119:5178-92 pubmed
    ..We found that Ccr4 cooperated with the Dun1 branch of the replication checkpoint, such that ccr4Delta dun1Delta strains exhibited irreversible hypersensitivity ..
  32. Fasullo M, Sun M, Egner P. Stimulation of sister chromatid exchanges and mutation by aflatoxin B1-DNA adducts in Saccharomyces cerevisiae requires MEC1 (ATR), RAD53, and DUN1. Mol Carcinog. 2008;47:608-15 pubmed publisher
    ..We found that MEC1, RAD53, and DUN1 are required for both AFB(1)-associated mutation and SCE...
  33. Karumbati A, Wilson T. Abrogation of the Chk1-Pds1 checkpoint leads to tolerance of persistent single-strand breaks in Saccharomyces cerevisiae. Genetics. 2005;169:1833-44 pubmed
    ..We propose a model in which recombinational repair during S phase coupled with failure of the metaphase-anaphase checkpoint allows for tolerance of persistent single-strand breaks at the expense of genome stability. ..
  34. Pijuan J, María C, Herrero E, Bellí G. Impaired mitochondrial Fe-S cluster biogenesis activates the DNA damage response through different signaling mediators. J Cell Sci. 2015;128:4653-65 pubmed publisher
    ..stage of the ISC machinery, different signaling pathway mediators contribute to such a response, converging on Dun1. Thus, cells lacking the glutaredoxin Grx5, which are compromised at the core ISC system, show Mec1- and Rad53-..
  35. Liang F, Wang Y. DNA damage checkpoints inhibit mitotic exit by two different mechanisms. Mol Cell Biol. 2007;27:5067-78 pubmed
    ..In contrast to previous data, the Rad53 pathway negatively regulates MEN independently of Cdc5, a Polo-like kinase essential for mitotic exit. Instead, a defective Rad53 pathway alleviates the inhibition of MEN by Bfa1. ..
  36. Myung K, Smith S, Kolodner R. Mitotic checkpoint function in the formation of gross chromosomal rearrangements in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2004;101:15980-5 pubmed
    ..These data suggest that functional mitotic checkpoints can play a role in the formation of genome rearrangements. ..
  37. Zhu Y, Xiao W. Two alternative cell cycle checkpoint pathways differentially control DNA damage-dependent induction of MAG1 and DDI1 expression in yeast. Mol Genet Genomics. 2001;266:436-44 pubmed
    ..MAG1 shares a common regulatory pathway with the RNR1,2,3,4 genes, which are also regulated by the POL2-MEC1-RAD53-DUN1 checkpoint pathway, and that the mec1-1 mutation probably represents a separation-of-function mutation...
  38. Caldwell J, Chen Y, Schollaert K, Theis J, Babcock G, Newlon C, et al. Orchestration of the S-phase and DNA damage checkpoint pathways by replication forks from early origins. J Cell Biol. 2008;180:1073-86 pubmed publisher
    ..Thus, oncogene-mediated deregulation of cyclins in the early stages of cancer development could contribute to genomic instability through a deficiency in the forks required to establish the S-phase checkpoint. ..
  39. Myung K, Pennaneach V, Kats E, Kolodner R. Saccharomyces cerevisiae chromatin-assembly factors that act during DNA replication function in the maintenance of genome stability. Proc Natl Acad Sci U S A. 2003;100:6640-5 pubmed
    ..These results indicate that coupling of chromatin assembly to DNA replication and DNA repair is critical to maintaining genome stability. ..
  40. Jack C, Cruz C, Hull R, Keller M, Ralser M, Houseley J. Regulation of ribosomal DNA amplification by the TOR pathway. Proc Natl Acad Sci U S A. 2015;112:9674-9 pubmed publisher
    ..Our results reveal how a signaling pathway can orchestrate specific genome changes and demonstrate that the copy number of repetitive DNA can be altered to suit environmental conditions. ..
  41. Williams L, Marjavaara L, Knowels G, Schultz E, Fox E, Chabes A, et al. dNTP pool levels modulate mutator phenotypes of error-prone DNA polymerase ? variants. Proc Natl Acad Sci U S A. 2015;112:E2457-66 pubmed publisher
    ..Deletion of DUN1 (dun1?) suppresses the mutator phenotype of pol2-4 (encoding Pol ? proofreading deficiency) and is synthetically ..
  42. Houseley J, Tollervey D. Repeat expansion in the budding yeast ribosomal DNA can occur independently of the canonical homologous recombination machinery. Nucleic Acids Res. 2011;39:8778-91 pubmed publisher
    ..Our data suggest that a novel mode of break-induced replication occurs in repetitive DNA that is dependent on high homology but does not require the canonical homologous recombination machinery. ..
  43. Lee S, Schwartz M, Duong J, Stern D. Rad53 phosphorylation site clusters are important for Rad53 regulation and signaling. Mol Cell Biol. 2003;23:6300-14 pubmed
    ..caused a decrease in DNA damage-induced Rad53 kinase activity and an impaired interaction with the protein kinase Dun1. The Dun1 FHA (Forkhead-associated) domain recognized the amino-terminal TQ cluster of Rad53 after DNA damage or ..
  44. Sanvisens N, Romero A, An X, Zhang C, de Llanos R, Martínez Pastor M, et al. Yeast Dun1 kinase regulates ribonucleotide reductase inhibitor Sml1 in response to iron deficiency. Mol Cell Biol. 2014;34:3259-71 pubmed publisher
    ..RNR large-subunit inhibitor, specifically decrease in response to both nutritional and genetic Fe deficiencies in a Dun1-dependent but Mec1/Rad53- and Aft1-independent manner...
  45. O Neill B, Szyjka S, Lis E, Bailey A, Yates J, Aparicio O, et al. Pph3-Psy2 is a phosphatase complex required for Rad53 dephosphorylation and replication fork restart during recovery from DNA damage. Proc Natl Acad Sci U S A. 2007;104:9290-5 pubmed
    ..These findings suggest that Rad53 regulates replication fork restart and initiation of late firing origins independently and that regulation of these processes is mediated by specific Rad53 phosphatases. ..
  46. Chen S, Zhou H. Reconstitution of Rad53 activation by Mec1 through adaptor protein Mrc1. J Biol Chem. 2009;284:18593-604 pubmed publisher
    ..Further, the conserved C-terminal domain of Mrc1 was found to be required for Rad53 activation. These results thus provide insights into the role of the adaptor protein Mrc1 in activating Rad53 in the DNA replication checkpoint. ..
  47. Mallory J, Bashkirov V, Trujillo K, Solinger J, Dominska M, Sung P, et al. Amino acid changes in Xrs2p, Dun1p, and Rfa2p that remove the preferred targets of the ATM family of protein kinases do not affect DNA repair or telomere length in Saccharomyces cerevisiae. DNA Repair (Amst). 2003;2:1041-64 pubmed
    ..We demonstrated that null alleles of DUN1 and certain mutant alleles of RFA2 result in short telomeres...
  48. Makovets S, Blackburn E. DNA damage signalling prevents deleterious telomere addition at DNA breaks. Nat Cell Biol. 2009;11:1383-6 pubmed publisher
    ..After DNA damage, MEC1-RAD53-DUN1-dependent phosphorylation of the telomerase inhibitor Pif1 occurs...
  49. Sanvisens N, Romero A, Zhang C, Wu X, An X, Huang M, et al. Yeast Dun1 Kinase Regulates Ribonucleotide Reductase Small Subunit Localization in Response to Iron Deficiency. J Biol Chem. 2016;291:9807-17 pubmed publisher
    ..Dif1 protein inhibits RNR by promoting nuclear import of Rnr2-Rnr4. Upon DNA damage, Dif1 phosphorylation by the Dun1 checkpoint kinase and its subsequent degradation enhances RNR function...
  50. Kaniak Golik A, Kuberska R, Dzierzbicki P, Sledziewska Gojska E. Activation of Dun1 in response to nuclear DNA instability accounts for the increase in mitochondrial point mutations in Rad27/FEN1 deficient S. cerevisiae. PLoS ONE. 2017;12:e0180153 pubmed publisher
    ..mutator phenotype resulting from Rad27 deficiency entirely depends on the DNA damage checkpoint kinase Dun1. DUN1 inactivation suppresses the mitochondrial mutator phenotype caused by Rad27 deficiency and this suppression ..
  51. Lee H, Yuan C, Hammet A, Mahajan A, Chen E, Wu M, et al. Diphosphothreonine-specific interaction between an SQ/TQ cluster and an FHA domain in the Rad53-Dun1 kinase cascade. Mol Cell. 2008;30:767-78 pubmed publisher
    ..The Rad53-SCD1 has dual functions in regulating the activation of the Rad53-Dun1 checkpoint kinase cascade but with unknown molecular mechanisms...
  52. Tang H, Siu K, Wong C, Jin D. Loss of yeast peroxiredoxin Tsa1p induces genome instability through activation of the DNA damage checkpoint and elevation of dNTP levels. PLoS Genet. 2009;5:e1000697 pubmed publisher
    ..Strong genetic interactions of TSA1 with DNA damage checkpoint components DUN1, SML1, and CRT1 were found when mutant cells were analyzed for either sensitivity to DNA damage or rate of ..