DSN1

Summary

Gene Symbol: DSN1
Description: MIND complex subunit DSN1
Alias: MIND complex subunit DSN1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Nekrasov V, Smith M, Peak Chew S, Kilmartin J. Interactions between centromere complexes in Saccharomyces cerevisiae. Mol Biol Cell. 2003;14:4931-46 pubmed
    ..These results show an increasingly complex structure for the S. cerevisiae centromere and a probable conservation of structure between parts of the centromeres of S. cerevisiae and S. pombe. ..
  2. Maskell D, Hu X, Singleton M. Molecular architecture and assembly of the yeast kinetochore MIND complex. J Cell Biol. 2010;190:823-34 pubmed publisher
    ..We propose a model for the overall structure of the complex and provide data on the interactions with NDC80, Spc105p, and thus the mitotic spindle. ..
  3. Akiyoshi B, Nelson C, Biggins S. The aurora B kinase promotes inner and outer kinetochore interactions in budding yeast. Genetics. 2013;194:785-9 pubmed publisher
    The kinetochore is the macromolecular protein complex that mediates chromosome segregation. The Dsn1 component is crucial for kinetochore assembly and is phosphorylated by the Aurora B kinase...
  4. Scharfenberger M, Ortiz J, Grau N, Janke C, Schiebel E, Lechner J. Nsl1p is essential for the establishment of bipolarity and the localization of the Dam-Duo complex. EMBO J. 2003;22:6584-97 pubmed
    ..Moreover the analysis of Nsl1p-depleted cells indicated that Nsl1p is required for the spindle checkpoint and kinetochore integrity. ..
  5. Corbett K, Yip C, Ee L, Walz T, Amon A, Harrison S. The monopolin complex crosslinks kinetochore components to regulate chromosome-microtubule attachments. Cell. 2010;142:556-67 pubmed publisher
    ..Csm1 presents a conserved hydrophobic surface patch that binds two kinetochore proteins: Dsn1, a subunit of the outer-kinetochore MIND/Mis12 complex, and Mif2/CENP-C...
  6. Bock L, Pagliuca C, Kobayashi N, Grove R, Oku Y, Shrestha K, et al. Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore. Nat Cell Biol. 2012;14:614-24 pubmed publisher
    ..Cnn1 regulates KMN activity in a spatiotemporal manner by inhibiting the interaction between its complexes. Cnn1 activity peaks in anaphase and is driven by the Cdc28, Mps1 and Ipl1 kinases. ..
  7. Peng Y, Wong C, Nakajima Y, Tyers R, Sarkeshik A, Yates J, et al. Overlapping kinetochore targets of CK2 and Aurora B kinases in mitotic regulation. Mol Biol Cell. 2011;22:2680-9 pubmed publisher
    ..Furthermore, we show Mif2 and the inner kinetochore protein Ndc10 are phosphorylated by CK2, and this phosphorylation plays antagonistic and synergistic roles with Aurora B phosphorylation of these targets, respectively. ..
  8. London N, Ceto S, Ranish J, Biggins S. Phosphoregulation of Spc105 by Mps1 and PP1 regulates Bub1 localization to kinetochores. Curr Biol. 2012;22:900-6 pubmed publisher
    ..Together, these data identify Spc105 as a key target of the Mps1 kinase and show that the opposing activities of Mps1 and PP1 regulate the kinetochore localization of the Bub1 protein...
  9. Pinsky B, Tatsutani S, Collins K, Biggins S. An Mtw1 complex promotes kinetochore biorientation that is monitored by the Ipl1/Aurora protein kinase. Dev Cell. 2003;5:735-45 pubmed
    ..We isolated mtw1-1 dosage suppressors and identified Dsn1, a kinetochore protein that immunoprecipitates with the Mif2/CENP-C and Cse4/CENP-A proteins, as well as the Mtw1, ..

More Information

Publications25

  1. Westermann S, Cheeseman I, Anderson S, Yates J, Drubin D, Barnes G. Architecture of the budding yeast kinetochore reveals a conserved molecular core. J Cell Biol. 2003;163:215-22 pubmed
    ..We propose that a molecular core consisting of CENP-A, -C, -H, and Ndc80/HEC has been conserved from yeast to humans to link centromeres to spindle microtubules. ..
  2. Hornung P, Maier M, Alushin G, Lander G, Nogales E, Westermann S. Molecular architecture and connectivity of the budding yeast Mtw1 kinetochore complex. J Mol Biol. 2011;405:548-59 pubmed publisher
    ..Ndc80 and Ctf19 complexes do not compete for binding to the Mtw1 complex, suggesting that Mtw1 can bridge the microtubule-binding components of the kinetochore to the inner centromere. ..
  3. London N, Biggins S. Mad1 kinetochore recruitment by Mps1-mediated phosphorylation of Bub1 signals the spindle checkpoint. Genes Dev. 2014;28:140-52 pubmed publisher
    ..The Mad1 interaction with Bub1 and kinetochores can be reconstituted in the presence of Mps1 and Mad2. Together, this work reveals a critical mechanism that determines kinetochore activation of the spindle checkpoint. ..
  4. Brito I, Monje Casas F, Amon A. The Lrs4-Csm1 monopolin complex associates with kinetochores during anaphase and is required for accurate chromosome segregation. Cell Cycle. 2010;9:3611-8 pubmed
    ..Furthermore, we find that Lrs4 and Csm1 are important for chromosome segregation fidelity. Our results reveal a previously unanticipated function for Lrs4-Csm1 in mitotic chromosome segregation. ..
  5. Euskirchen G. Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a new group of proteins important for chromosome segregation in Saccharomyces cerevisiae. Eukaryot Cell. 2002;1:229-40 pubmed
    ..I have identified the novel essential genes DSN1, DSN3, and NSL1 through genetic interactions with nnf1-17...
  6. Sarangapani K, Akiyoshi B, Duggan N, Biggins S, Asbury C. Phosphoregulation promotes release of kinetochores from dynamic microtubules via multiple mechanisms. Proc Natl Acad Sci U S A. 2013;110:7282-7 pubmed publisher
    ..Taken together, our results suggest that, in vivo, Aurora B releases kinetochores via at least two mechanisms: by weakening the kinetochore-microtubule interface and also by destabilizing the kinetochore-attached microtubule tip...
  7. Samel A, Nguyen T, Ehrenhofer Murray A. Defects in methylation of arginine 37 on CENP-A/Cse4 are compensated by the ubiquitin ligase complex Ubr2/Mub1. FEMS Yeast Res. 2017;17: pubmed publisher
    ..Ubr2 is known to regulate the levels of the MIND complex component Dsn1 via ubiquitination and proteasome-mediated degradation...
  8. Sarkar S, Shenoy R, Dalgaard J, Newnham L, Hoffmann E, Millar J, et al. Monopolin subunit Csm1 associates with MIND complex to establish monopolar attachment of sister kinetochores at meiosis I. PLoS Genet. 2013;9:e1003610 pubmed publisher
    ..Furthermore, we demonstrate that interaction of monopolin subunit Csm1 with the N-terminal domain of MIND complex subunit Dsn1, is essential for both the association of monopolin with kinetochores and for monopolar attachment of ..
  9. Hewawasam G, Mattingly M, Venkatesh S, Zhang Y, Florens L, Workman J, et al. Phosphorylation by casein kinase 2 facilitates Psh1 protein-assisted degradation of Cse4 protein. J Biol Chem. 2014;289:29297-309 pubmed publisher
    ..Collectively our data suggest that phosphorylation is crucial in Psh1-assisted control of Cse4 levels and that the Psh1-Cse4 association itself functions to prevent Cse4 misincorporation. ..
  10. Meyer R, Chuong H, Hild M, Hansen C, Kinter M, Dawson D. Ipl1/Aurora-B is necessary for kinetochore restructuring in meiosis I in Saccharomyces cerevisiae. Mol Biol Cell. 2015;26:2986-3000 pubmed publisher
    ..This shedding is regulated by the conserved protein kinase Ipl1/Aurora-B and promotes the subsequent assembly of a kinetochore that will confer meiosis-specific segregation patterns on the chromosome. ..
  11. Hornung P, Troć P, Malvezzi F, Maier M, Demianova Z, Zimniak T, et al. A cooperative mechanism drives budding yeast kinetochore assembly downstream of CENP-A. J Cell Biol. 2014;206:509-24 pubmed publisher
    ..This two-step mechanism may protect against inappropriate kinetochore assembly similar to rate-limiting nucleation steps used by cytoskeletal polymers. ..
  12. Deyter G, Hildebrand E, Barber A, Biggins S. Histone H4 Facilitates the Proteolysis of the Budding Yeast CENP-ACse4 Centromeric Histone Variant. Genetics. 2017;205:113-124 pubmed publisher
    ..Together, these data reveal a key residue in histone H4 that is important for efficient CENP-ACse4 degradation, likely by facilitating the interaction between Psh1 and CENP-ACse4. ..
  13. Akiyoshi B, Nelson C, Duggan N, Ceto S, Ranish J, Biggins S. The Mub1/Ubr2 ubiquitin ligase complex regulates the conserved Dsn1 kinetochore protein. PLoS Genet. 2013;9:e1003216 pubmed publisher
    ..We previously developed a method to isolate kinetochore particles via purification of the conserved Dsn1 kinetochore protein...
  14. Miller M, Asbury C, Biggins S. A TOG Protein Confers Tension Sensitivity to Kinetochore-Microtubule Attachments. Cell. 2016;165:1428-1439 pubmed publisher
  15. Aravamudhan P, Felzer Kim I, Gurunathan K, Joglekar A. Assembling the protein architecture of the budding yeast kinetochore-microtubule attachment using FRET. Curr Biol. 2014;24:1437-46 pubmed publisher
  16. Ye Q, Ur S, Su T, Corbett K. Structure of the Saccharomyces cerevisiae Hrr25:Mam1 monopolin subcomplex reveals a novel kinase regulator. EMBO J. 2016;35:2139-2151 pubmed
    ..Finally, using purified proteins, we find that Hrr25 phosphorylates the kinetochore receptor for monopolin, Dsn1. Together with our new structural insights into the fully assembled monopolin complex, this finding suggests that ..