Gene Symbol: Dsl1p
Description: Dsl1p
Alias: RNS1, Dsl1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Diefenbacher M, Thorsteinsdóttir H, Spang A. The Dsl1 tethering complex actively participates in soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor (SNARE) complex assembly at the endoplasmic reticulum in Saccharomyces cerevisiae. J Biol Chem. 2011;286:25027-38 pubmed publisher
    ..Golgi-derived vesicles to the ER is the Dsl1 tethering complex, which is comprised of the essential proteins Dsl1p, Dsl3p, and Tip20p...
  2. Andag U, Neumann T, Schmitt H. The coatomer-interacting protein Dsl1p is required for Golgi-to-endoplasmic reticulum retrieval in yeast. J Biol Chem. 2001;276:39150-60 pubmed
    ..Furthermore, we demonstrate that Dsl1p is a peripheral membrane protein, which in vitro specifically binds to coatomer, the major component of the ..
  3. Reilly B, Kraynack B, VanRheenen S, Waters M. Golgi-to-endoplasmic reticulum (ER) retrograde traffic in yeast requires Dsl1p, a component of the ER target site that interacts with a COPI coat subunit. Mol Biol Cell. 2001;12:3783-96 pubmed
    ..These results suggest that the primary function of Dsl1p may be in retrograde traffic, and that retrograde defects can lead to secondary defects in anterograde traffic...
  4. VanRheenen S, Reilly B, Chamberlain S, Waters M. Dsl1p, an essential protein required for membrane traffic at the endoplasmic reticulum/Golgi interface in yeast. Traffic. 2001;2:212-31 pubmed
    ..Because the strongest suppressor is SEC21, we proposed that Dsl1p functions primarily in retrograde Golgi to ER traffic, although it is possible that Dsl1p functions in anterograde ..
  5. Andag U, Schmitt H. Dsl1p, an essential component of the Golgi-endoplasmic reticulum retrieval system in yeast, uses the same sequence motif to interact with different subunits of the COPI vesicle coat. J Biol Chem. 2003;278:51722-34 pubmed
    b>Dsl1p is required for Golgi-endoplasmic reticulum (ER) retrograde transport in yeast. It interacts with the ER resident protein Tip20p and with delta-COP, a subunit of coatomer, the coat complex of COPI vesicles...
  6. Kraynack B, Chan A, Rosenthal E, Essid M, Umansky B, Waters M, et al. Dsl1p, Tip20p, and the novel Dsl3(Sec39) protein are required for the stability of the Q/t-SNARE complex at the endoplasmic reticulum in yeast. Mol Biol Cell. 2005;16:3963-77 pubmed
    The "Dsl1p complex" in Saccharomyces cerevisiae, consisting of Dsl1p and Tip20p, is involved in Golgi-ER retrograde transport and it is functionally conserved from yeast to mammalian cells...
  7. Zink S, Wenzel D, Wurm C, Schmitt H. A link between ER tethering and COP-I vesicle uncoating. Dev Cell. 2009;17:403-16 pubmed publisher
    The yeast Dsl1p vesicle tethering complex, comprising the three subunits Dsl1p, Dsl3p, and Tip20p, is stably associated with three endoplasmic reticulum-localized Q-SNAREs and is believed to play a central role in the tethering and ..
  8. Suckling R, Poon P, Travis S, Majoul I, Hughson F, Evans P, et al. Structural basis for the binding of tryptophan-based motifs by δ-COP. Proc Natl Acad Sci U S A. 2015;112:14242-7 pubmed publisher
    ..vesicles; this conclusion is supported by the observation that, in the context of a sensitizing domain deletion in Dsl1p, mutating the tryptophan-based motif-binding site in yeast causes defects in both growth and carboxypeptidase Y ..
  9. Li Y, Gallwitz D, Peng R. Structure-based functional analysis reveals a role for the SM protein Sly1p in retrograde transport to the endoplasmic reticulum. Mol Biol Cell. 2005;16:3951-62 pubmed
    ..Together, these results indicate a previously unrecognized function of Sly1p in retrograde transport to the endoplasmic reticulum. ..

More Information


  1. Ren Y, Yip C, Tripathi A, Huie D, Jeffrey P, Walz T, et al. A structure-based mechanism for vesicle capture by the multisubunit tethering complex Dsl1. Cell. 2009;139:1119-29 pubmed publisher
    ..Our results suggest that even the simplest MTC may be capable of orchestrating vesicle capture, uncoating, and fusion...
  2. Hsia K, Hoelz A. Crystal structure of alpha-COP in complex with epsilon-COP provides insight into the architecture of the COPI vesicular coat. Proc Natl Acad Sci U S A. 2010;107:11271-6 pubmed publisher
    ..These data suggest that the heterodimer is exposed on COPI vesicles, while the remaining part of the B-subcomplex oligomerizes underneath into a cage. ..
  3. Ishikawa T, Unno K, Nonaka G, Nakajima H, Kitamoto K. Isolation of Saccharomyces cerevisiae RNase T1 hypersensitive (rns) mutants and genetic analysis of the RNS1/DSL1 gene. J Gen Appl Microbiol. 2005;51:73-82 pubmed
    ..We identified the genes mutated in three of the rns mutants, rns1, rns2, and rns3, as DSL1, UMP1, and SEC17, respectively...
  4. Tripathi A, Ren Y, Jeffrey P, Hughson F. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nat Struct Mol Biol. 2009;16:114-23 pubmed publisher
    ..Here we report initial structural characterization of the Dsl1p complex, whose three subunits are essential for trafficking from the Golgi apparatus to the endoplasmic reticulum (..
  5. Schmitt H. Dsl1p/Zw10: common mechanisms behind tethering vesicles and microtubules. Trends Cell Biol. 2010;20:257-68 pubmed publisher
    ..The complex seems to be designed to expose an unstructured domain of Dsl1p at its top, which is required to capture vesicles...
  6. Meiringer C, Rethmeier R, Auffarth K, Wilson J, Perz A, Barlowe C, et al. The Dsl1 protein tethering complex is a resident endoplasmic reticulum complex, which interacts with five soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptors (SNAREs): implications for fusion and fusion regulation. J Biol Chem. 2011;286:25039-46 pubmed publisher
    ..Our data support a model, in which a tethering complex is stabilized at the organelle membrane by binding to SNAREs, recognizes the incoming vesicle via its coat and then promotes its SNARE-mediated fusion. ..
  7. Perry R, Mast F, Rachubinski R. Endoplasmic reticulum-associated secretory proteins Sec20p, Sec39p, and Dsl1p are involved in peroxisome biogenesis. Eukaryot Cell. 2009;8:830-43 pubmed publisher
    ..protein chimera Pex3p-GFP localized to tubular-vesicular structures in cells suppressed for Sec20p, Sec39p, and Dsl1p, which form a complex at the ER...