Genomes and Genes
Gene Symbol: DPB2
Description: DNA polymerase epsilon noncatalytic subunit
Alias: DNA polymerase epsilon noncatalytic subunit
Species: Saccharomyces cerevisiae S288c
- Tsubota T, Maki S, Kubota H, Sugino A, Maki H. Double-stranded DNA binding properties of Saccharomyces cerevisiae DNA polymerase epsilon and of the Dpb3p-Dpb4p subassembly. Genes Cells. 2003;8:873-88 pubmed..One of these sites has a strong affinity for dsDNA, a feature that is not generally associated with DNA polymerases. Involvement of the Dpb3p-Dpb4p complex in the dsDNA-binding of Pol epsilon is inferred. ..
- Shcherbakova P, Pavlov Y, Chilkova O, Rogozin I, Johansson E, Kunkel T. Unique error signature of the four-subunit yeast DNA polymerase epsilon. J Biol Chem. 2003;278:43770-80 pubmed..We discuss the implications of these findings for the role of Pol epsilon polymerase activity in DNA replication. ..
- Edwards S, Li C, Levy D, Brown J, Snow P, Campbell J. Saccharomyces cerevisiae DNA polymerase epsilon and polymerase sigma interact physically and functionally, suggesting a role for polymerase epsilon in sister chromatid cohesion. Mol Cell Biol. 2003;23:2733-48 pubmed..We also show that trf5 Delta mutants, like trf4 Delta mutants, are defective in DNA repair and sister chromatid cohesion. ..
- Dua R, Levy D, Campbell J. Role of the putative zinc finger domain of Saccharomyces cerevisiae DNA polymerase epsilon in DNA replication and the S/M checkpoint pathway. J Biol Chem. 1998;273:30046-55 pubmed..The POL2 C terminus is sufficient for interaction with DPB2, the essential and phylogenetically conserved subunit of pol epsilon, but not for interaction with DPB3...
- Burgers P. Eukaryotic DNA polymerases in DNA replication and DNA repair. Chromosoma. 1998;107:218-27 pubmed..The role of DNA polymerase beta in base-excision repair is well established for mammalian systems, but in yeast, DNA polymerase delta appears to fulfill that function. ..
- Ohya T, Kawasaki Y, Hiraga S, Kanbara S, Nakajo K, Nakashima N, et al. The DNA polymerase domain of pol(epsilon) is required for rapid, efficient, and highly accurate chromosomal DNA replication, telomere length maintenance, and normal cell senescence in Saccharomyces cerevisiae. J Biol Chem. 2002;277:28099-108 pubmed..These results indicate that the DNA polymerase domain of Pol2p is required for rapid, efficient, and highly accurate chromosomal DNA replication in yeast. ..
- Ohya T, Maki S, Kawasaki Y, Sugino A. Structure and function of the fourth subunit (Dpb4p) of DNA polymerase epsilon in Saccharomyces cerevisiae. Nucleic Acids Res. 2000;28:3846-52 pubmed..The genes POL2, DPB2 and DPB3, encoding the catalytic Pol2p, the second (Dpb2p) and the third largest subunits (Dpb3p) of the complex, ..
- Kesti T, McDonald W, Yates J, Wittenberg C. Cell cycle-dependent phosphorylation of the DNA polymerase epsilon subunit, Dpb2, by the Cdc28 cyclin-dependent protein kinase. J Biol Chem. 2004;279:14245-55 pubmed..of the essential catalytic subunit, called Pol2 in budding yeast, and three accessory subunits, only one of which, Dpb2, is essential...
- Kraszewska J, Garbacz M, Jonczyk P, Fijalkowska I, Jaszczur M. Defect of Dpb2p, a noncatalytic subunit of DNA polymerase ?, promotes error prone replication of undamaged chromosomal DNA in Saccharomyces cerevisiae. Mutat Res. 2012;737:34-42 pubmed publisher..The strength of the mutator phenotype due to the different dpb2 alleles was inversely proportional to the strength of protein-protein interactions between Pol2p and the mutated ..
- Muramatsu S, Hirai K, Tak Y, Kamimura Y, Araki H. CDK-dependent complex formation between replication proteins Dpb11, Sld2, Pol (epsilon}, and GINS in budding yeast. Genes Dev. 2010;24:602-12 pubmed publisher..We propose that CDK regulates the initiation of DNA replication in budding yeast through formation of the pre-LC. ..
- Jaszczur M, Rudzka J, Kraszewska J, Flis K, Polaczek P, Campbell J, et al. Defective interaction between Pol2p and Dpb2p, subunits of DNA polymerase epsilon, contributes to a mutator phenotype in Saccharomyces cerevisiae. Mutat Res. 2009;669:27-35 pubmed publisher..cerevisiae strains carrying different mutated alleles of the DPB2 gene...
- Dmowski M, Rudzka J, Campbell J, Jonczyk P, Fijałkowska I. Mutations in the Non-Catalytic Subunit Dpb2 of DNA Polymerase Epsilon Affect the Nrm1 Branch of the DNA Replication Checkpoint. PLoS Genet. 2017;13:e1006572 pubmed publisher..Here we have studied the involvement of Pol ? in sensing proper progression of DNA replication, using a mutant in DPB2, the gene coding for a non-catalytic subunit of Pol ?...
- Langston L, Zhang D, Yurieva O, Georgescu R, Finkelstein J, Yao N, et al. CMG helicase and DNA polymerase Îµ form a functional 15-subunit holoenzyme for eukaryotic leading-strand DNA replication. Proc Natl Acad Sci U S A. 2014;111:15390-5 pubmed publisher..On its own, the Pol2 catalytic subunit of Pol Îµ is inefficient in CMG-dependent replication, but addition of the Dpb2 protein subunit of Pol Îµ, known to bind the Psf1 protein subunit of CMG, allows stable synthesis with CMG...
- Ganai R, Osterman P, Johansson E. Yeast DNA polymerase Ïµ catalytic core and holoenzyme have comparable catalytic rates. J Biol Chem. 2015;290:3825-35 pubmed publisherThe holoenzyme of yeast DNA polymerase Ïµ (Pol Ïµ) consists of four subunits: Pol2, Dpb2, Dpb3, and Dpb4...
- Garbacz M, Araki H, Flis K, Bebenek A, Zawada A, Jonczyk P, et al. Fidelity consequences of the impaired interaction between DNA polymerase epsilon and the GINS complex. DNA Repair (Amst). 2015;29:23-35 pubmed publisher..It is postulated that CMG is responsible for targeting of Pol É› to the leading strand. We isolated a mutator dpb2-100 allele which encodes the mutant form of Dpb2p...
- Yaakov G, Duch A, Garcia Rubio M, Clotet J, Jimenez J, Aguilera A, et al. The stress-activated protein kinase Hog1 mediates S phase delay in response to osmostress. Mol Biol Cell. 2009;20:3572-82 pubmed publisher..Hog1 interacts with components of the replication complex and delays phosphorylation of the Dpb2 subunit of the DNA polymerase...
- Bloom J, Cross F. Novel role for Cdc14 sequestration: Cdc14 dephosphorylates factors that promote DNA replication. Mol Cell Biol. 2007;27:842-53 pubmed..it efficiently dephosphorylates a subset of Cdk1 substrates including the replication factors, Sld2 and Dpb2. Mutations causing Cdc14 mislocalization interact genetically with mutations affecting the function of DNA ..
- Kawasaki Y, Hiraga S, Sugino A. Interactions between Mcm10p and other replication factors are required for proper initiation and elongation of chromosomal DNA replication in Saccharomyces cerevisiae. Genes Cells. 2000;5:975-89 pubmed
- Araki H, Leem S, Phongdara A, Sugino A. Dpb11, which interacts with DNA polymerase II(epsilon) in Saccharomyces cerevisiae, has a dual role in S-phase progression and at a cell cycle checkpoint. Proc Natl Acad Sci U S A. 1995;92:11791-5 pubmed..mutations in two essential subunits of Saccharomyces cerevisiae DNA polymerase II(epsilon) encoded by POL2 and DPB2, was isolated on a multicopy plasmid...
- Lou H, Komata M, Katou Y, Guan Z, Reis C, Budd M, et al. Mrc1 and DNA polymerase epsilon function together in linking DNA replication and the S phase checkpoint. Mol Cell. 2008;32:106-17 pubmed publisher..We propose that Mrc1, which also interacts with the MCMs, may modulate coupling of polymerization and unwinding at the replication fork. ..
- Tanaka H, Katou Y, Yagura M, Saitoh K, Itoh T, Araki H, et al. Ctf4 coordinates the progression of helicase and DNA polymerase alpha. Genes Cells. 2009;14:807-20 pubmed publisher..These results lead us to propose that Ctf4 is a key connector between DNA helicase and Pol alpha and is required for the coordinated progression of the replisome. ..
- Tsubota T, Tajima R, Ode K, Kubota H, Fukuhara N, Kawabata T, et al. Double-stranded DNA binding, an unusual property of DNA polymerase epsilon, promotes epigenetic silencing in Saccharomyces cerevisiae. J Biol Chem. 2006;281:32898-908 pubmed..We conclude that the dsDNA-binding property of Pol epsilon is required for epigenetic silencing at telomeres. ..
- Tackett A, Dilworth D, Davey M, O DONNELL M, Aitchison J, Rout M, et al. Proteomic and genomic characterization of chromatin complexes at a boundary. J Cell Biol. 2005;169:35-47 pubmed..We show that these complexes are important for the faithful maintenance of an established boundary, as disruption of the complexes results in specific, anomalous alterations of the silent and active epigenetic states. ..
- Sundin B, Chiu C, Riffle M, Davis T, Muller E. Localization of proteins that are coordinately expressed with Cln2 during the cell cycle. Yeast. 2004;21:793-800 pubmed..A complete list of localizations, along with images, can be found at our website (http://www.yeastrc.org/cln2/). ..
- Zou L, Stillman B. Assembly of a complex containing Cdc45p, replication protein A, and Mcm2p at replication origins controlled by S-phase cyclin-dependent kinases and Cdc7p-Dbf4p kinase. Mol Cell Biol. 2000;20:3086-96 pubmed..These results suggest that the complex containing Cdc45p, RPA, and MCMs is involved in origin unwinding and assembly of replication forks at each origin. ..
- Okimoto H, Tanaka S, Araki H, Ohashi E, Tsurimoto T. Conserved interaction of Ctf18-RFC with DNA polymerase ? is critical for maintenance of genome stability in Saccharomyces cerevisiae. Genes Cells. 2016;21:482-91 pubmed publisher..These results indicate that the Ctf18-RFC/Pol? interaction plays a crucial role in maintaining genome stability in budding yeast, probably through recruitment of this PCNA loader to the replication fork. ..
- Villa F, Simon A, Ortiz Bazan M, Kilkenny M, Wirthensohn D, Wightman M, et al. Ctf4 Is a Hub in the Eukaryotic Replisome that Links Multiple CIP-Box Proteins to the CMG Helicase. Mol Cell. 2016;63:385-96 pubmed publisher..Most strikingly, Ctf4-dependent recruitment of CIP-box proteins couples other processes to DNA synthesis, including rDNA copy-number regulation. ..
- Komata M, Bando M, Araki H, Shirahige K. The direct binding of Mrc1, a checkpoint mediator, to Mcm6, a replication helicase, is essential for the replication checkpoint against methyl methanesulfonate-induced stress. Mol Cell Biol. 2009;29:5008-19 pubmed publisher..These results strongly suggest for the first time that an Mcm helicase acts as a checkpoint sensor for methyl methanesulfonate-induced DNA damage through direct binding to the replication checkpoint mediator Mrc1. ..
- Isoz I, Persson U, Volkov K, Johansson E. The C-terminus of Dpb2 is required for interaction with Pol2 and for cell viability. Nucleic Acids Res. 2012;40:11545-53 pubmed publisher..Pol ? comprises four subunits: the catalytic subunit, Pol2, and three accessory subunits, Dpb2, Dpb3 and Dpb4. DPB2 is an essential gene with unclear function. A genetic screen was performed in S...
- Sengupta S, van Deursen F, De Piccoli G, Labib K. Dpb2 integrates the leading-strand DNA polymerase into the eukaryotic replisome. Curr Biol. 2013;23:543-52 pubmed publisher..Here, we use budding yeast to show that a conserved amino-terminal domain of the Dpb2 subunit of Pol ? (Dpb2NT) interacts with the Psf1 component of GINS, via the unique "B domain" of the ..
- Pavlov Y, Maki S, Maki H, Kunkel T. Evidence for interplay among yeast replicative DNA polymerases alpha, delta and epsilon from studies of exonuclease and polymerase active site mutations. BMC Biol. 2004;2:11 pubmed..This suggests that other polymerases can substitute for certain functions of polymerase epsilon. ..
- Kolodner R, Marsischky G. Eukaryotic DNA mismatch repair. Curr Opin Genet Dev. 1999;9:89-96 pubmed..MMR proteins function in these processes in conjunction with components of nucleotide excision repair (NER) and, possibly, recombination. ..