DNM1

Summary

Gene Symbol: DNM1
Description: dynamin-related GTPase DNM1
Alias: dynamin-related GTPase DNM1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Cerveny K, Jensen R. The WD-repeats of Net2p interact with Dnm1p and Fis1p to regulate division of mitochondria. Mol Biol Cell. 2003;14:4126-39 pubmed
    The Net2, Fis1, and Dnm1 proteins are required for the division of mitochondria in the yeast Saccharomyces cerevisiae...
  2. Koirala S, Bui H, Schubert H, Eckert D, Hill C, Kay M, et al. Molecular architecture of a dynamin adaptor: implications for assembly of mitochondrial fission complexes. J Cell Biol. 2010;191:1127-39 pubmed publisher
    ..Subsequent Mdv1 binding to the mitochondrial dynamin Dnm1 stimulates Dnm1 assembly into spirals, which encircle and divide the mitochondrial compartment...
  3. Fekkes P, Shepard K, Yaffe M. Gag3p, an outer membrane protein required for fission of mitochondrial tubules. J Cell Biol. 2000;151:333-40 pubmed
    ..The gag1 mutation mapped to DNM1 that encodes a dynamin-related protein required for mitochondrial fission...
  4. Naylor K, Ingerman E, Okreglak V, Marino M, Hinshaw J, Nunnari J. Mdv1 interacts with assembled dnm1 to promote mitochondrial division. J Biol Chem. 2006;281:2177-83 pubmed
    The dynamin-related GTPase, Dnm1, self-assembles into punctate structures that are targeted to the outer mitochondrial membrane where they mediate mitochondrial division...
  5. Lackner L, Horner J, Nunnari J. Mechanistic analysis of a dynamin effector. Science. 2009;325:874-7 pubmed publisher
    ..the mechanistic role of a DAP, we used the yeast mitochondrial division machine as a model, which requires the DRP Dnm1, and two other proteins, Mdv1 and Fis1...
  6. Otsuga D, Keegan B, Brisch E, Thatcher J, Hermann G, Bleazard W, et al. The dynamin-related GTPase, Dnm1p, controls mitochondrial morphology in yeast. J Cell Biol. 1998;143:333-49 pubmed
    The Saccharomyces cerevisiae Dnm1 protein is structurally related to dynamin, a GTPase required for membrane scission during endocytosis. Here we show that Dnm1p is essential for the maintenance of mitochondrial morphology...
  7. Karren M, Coonrod E, Anderson T, Shaw J. The role of Fis1p-Mdv1p interactions in mitochondrial fission complex assembly. J Cell Biol. 2005;171:291-301 pubmed
    ..These studies suggest that the concave binding surface of the Fis1p TPR-like fold interacts with Mdv1p during mitochondrial fission and that Mdv1p facilitates Dnm1p recruitment into functional fission complexes. ..
  8. Sesaki H, Southard S, Hobbs A, Jensen R. Cells lacking Pcp1p/Ugo2p, a rhomboid-like protease required for Mgm1p processing, lose mtDNA and mitochondrial structure in a Dnm1p-dependent manner, but remain competent for mitochondrial fusion. Biochem Biophys Res Commun. 2003;308:276-83 pubmed
    ..However, the processing of Mgm1p is not strictly required for mitochondrial fusion, indicating that the 100 kDa form is sufficient to promote fusion. ..
  9. Griffin E, Graumann J, Chan D. The WD40 protein Caf4p is a component of the mitochondrial fission machinery and recruits Dnm1p to mitochondria. J Cell Biol. 2005;170:237-48 pubmed
    ..Our studies support a revised model for assembly of the mitochondrial fission apparatus. ..

More Information

Publications48

  1. Bhar D, Karren M, Babst M, Shaw J. Dimeric Dnm1-G385D interacts with Mdv1 on mitochondria and can be stimulated to assemble into fission complexes containing Mdv1 and Fis1. J Biol Chem. 2006;281:17312-20 pubmed
    ..Fis1p complexes. These dimers then assemble into multimers that subsequently promote the reorganization of Mdv1p into punctate fission complexes. ..
  2. Bleazard W, McCaffery J, King E, Bale S, Mozdy A, Tieu Q, et al. The dynamin-related GTPase Dnm1 regulates mitochondrial fission in yeast. Nat Cell Biol. 1999;1:298-304 pubmed
    The dynamin-related GTPase Dnm1 controls mitochondrial morphology in yeast. Here we show that dnm1 mutations convert the mitochondrial compartment into a planar 'net' of interconnected tubules...
  3. Tieu Q, Nunnari J. Mdv1p is a WD repeat protein that interacts with the dynamin-related GTPase, Dnm1p, to trigger mitochondrial division. J Cell Biol. 2000;151:353-66 pubmed
  4. Cerveny K, McCaffery J, Jensen R. Division of mitochondria requires a novel DMN1-interacting protein, Net2p. Mol Biol Cell. 2001;12:309-21 pubmed
    ..a single mitochondrion that consists of a network formed by interconnected tubules, similar to the phenotype of dnm1 Delta cells...
  5. Amiott E, Cohen M, Saint Georges Y, Weissman A, Shaw J. A mutation associated with CMT2A neuropathy causes defects in Fzo1 GTP hydrolysis, ubiquitylation, and protein turnover. Mol Biol Cell. 2009;20:5026-35 pubmed publisher
  6. Cerveny K, Studer S, Jensen R, Sesaki H. Yeast mitochondrial division and distribution require the cortical num1 protein. Dev Cell. 2007;12:363-75 pubmed
    Yeast mitochondrial division requires the dynamin-related Dnm1 protein...
  7. Mozdy A, McCaffery J, Shaw J. Dnm1p GTPase-mediated mitochondrial fission is a multi-step process requiring the novel integral membrane component Fis1p. J Cell Biol. 2000;151:367-80 pubmed
    ..We propose that mitochondrial fission in yeast is a multi-step process, and that membrane-bound Fis1p is required for the proper assembly, membrane distribution, and function of Dnm1p-containing complexes during fission. ..
  8. Schauss A, Bewersdorf J, Jakobs S. Fis1p and Caf4p, but not Mdv1p, determine the polar localization of Dnm1p clusters on the mitochondrial surface. J Cell Sci. 2006;119:3098-106 pubmed
    ..This study suggests that Caf4p plays a key role in determining the polarized localization of those Dnm1p clusters that are not immediately involved in the mitochondrial fission process. ..
  9. Sesaki H, Southard S, Yaffe M, Jensen R. Mgm1p, a dynamin-related GTPase, is essential for fusion of the mitochondrial outer membrane. Mol Biol Cell. 2003;14:2342-56 pubmed
    ..Fragmentation of mitochondria in mgm1 mutants is rescued by disrupting DNM1, a gene required for mitochondrial division...
  10. Tieu Q, Okreglak V, Naylor K, Nunnari J. The WD repeat protein, Mdv1p, functions as a molecular adaptor by interacting with Dnm1p and Fis1p during mitochondrial fission. J Cell Biol. 2002;158:445-52 pubmed
  11. Fukushima N, Brisch E, Keegan B, Bleazard W, Shaw J. The GTPase effector domain sequence of the Dnm1p GTPase regulates self-assembly and controls a rate-limiting step in mitochondrial fission. Mol Biol Cell. 2001;12:2756-66 pubmed
    ..Here we show that expression of the Dnm1 K705A protein in yeast enhances mitochondrial fission...
  12. Kuravi K, Nagotu S, Krikken A, Sjollema K, Deckers M, Erdmann R, et al. Dynamin-related proteins Vps1p and Dnm1p control peroxisome abundance in Saccharomyces cerevisiae. J Cell Sci. 2006;119:3994-4001 pubmed
    ..Previous data from glucose-grown VPS1 and DNM1 null mutants suggested that Vps1p, but not Dnm1p, plays a role in regulating peroxisome abundance...
  13. Ingerman E, Perkins E, Marino M, Mears J, McCaffery J, Hinshaw J, et al. Dnm1 forms spirals that are structurally tailored to fit mitochondria. J Cell Biol. 2005;170:1021-7 pubmed
    ..b>Dnm1, which is a yeast DRP (Drp1/Dlp1 in humans), is required for mitochondrial division, but its mechanism is unknown...
  14. Sesaki H, Jensen R. Division versus fusion: Dnm1p and Fzo1p antagonistically regulate mitochondrial shape. J Cell Biol. 1999;147:699-706 pubmed
    ..These mutants, which appear defective in mitochondrial division, all carried mutations in DNM1, a dynamin-related protein that localizes to mitochondria...
  15. Fritz S, Weinbach N, Westermann B. Mdm30 is an F-box protein required for maintenance of fusion-competent mitochondria in yeast. Mol Biol Cell. 2003;14:2303-13 pubmed
    ..These defects are rescued by deletion of DNM1, a gene encoding a component of the mitochondrial division machinery...
  16. Teixeira V, Medeiros T, Vilaça R, Pereira A, Chaves S, Côrte Real M, et al. Ceramide signalling impinges on Sit4p and Hog1p to promote mitochondrial fission and mitophagy in Isc1p-deficient cells. Cell Signal. 2015;27:1840-9 pubmed publisher
    ..high levels of Dnm1p associated with unbalanced mitochondrial fission, leading to mitochondrial fragmentation, and DNM1 deletion suppressed the oxidative stress sensitivity and shortened lifespan of isc1Δ cells...
  17. Motley A, Ward G, Hettema E. Dnm1p-dependent peroxisome fission requires Caf4p, Mdv1p and Fis1p. J Cell Sci. 2008;121:1633-40 pubmed publisher
    ..vacuolar fusion defect of vps1 cells and Vps1p overexpression does not restore the mitochondrial fission defect of dnm1 cells. These data show that Vps1p and Dnm1p are part of independent fission machineries...
  18. Prevost C, Peris N, Seger C, Pedeville D, Wershing K, Sia E, et al. The influence of mitochondrial dynamics on mitochondrial genome stability. Curr Genet. 2018;64:199-214 pubmed publisher
    ..While increased loss of mtDNA integrity has been demonstrated for dnm1-? fission mutants after growth in a non-fermentable carbon source, we demonstrate that growth of yeast in different ..
  19. Harner M, Unger A, Geerts W, Mari M, Izawa T, Stenger M, et al. An evidence based hypothesis on the existence of two pathways of mitochondrial crista formation. elife. 2016;5: pubmed publisher
    ..The proposed hypothesis is suggested to apply also to higher eukaryotes, since the key components are conserved in structure and function throughout evolution. ..
  20. Joshi A, Fei N, Greenberg M. Get1p and Get2p are required for maintenance of mitochondrial morphology and normal cardiolipin levels. FEMS Yeast Res. 2016;16: pubmed publisher
    ..The genetic interactions observed in this study identify novel physiological modifiers that are required for maintenance of CL levels and mitochondrial morphology. ..
  21. Cooper K, Khakhina S, Kim S, Strich R. Stress-induced nuclear-to-cytoplasmic translocation of cyclin C promotes mitochondrial fission in yeast. Dev Cell. 2014;28:161-73 pubmed publisher
    ..This study provides a mechanism by which stress-induced gene induction and mitochondrial fission are coordinated through translocation of cyclin C. ..
  22. Lackner L, Ping H, Graef M, Murley A, Nunnari J. Endoplasmic reticulum-associated mitochondria-cortex tether functions in the distribution and inheritance of mitochondria. Proc Natl Acad Sci U S A. 2013;110:E458-67 pubmed publisher
    ..dynamics in vivo, we identified genes that become essential in cells lacking the dynamin-related proteins Fzo1 and Dnm1, which are required for mitochondrial fusion and division, respectively...
  23. Rucktäschel R, Halbach A, Girzalsky W, Rottensteiner H, Erdmann R. De novo synthesis of peroxisomes upon mitochondrial targeting of Pex3p. Eur J Cell Biol. 2010;89:947-54 pubmed publisher
    ..We conclude that natural or artificial targeting of Pex3p to any endomembrane may initiate peroxisome formation and that also Pex3p-containing mitochondria can serve as source for the de novo synthesis of peroxisomes. ..
  24. Mao K, Liu X, Feng Y, Klionsky D. The progression of peroxisomal degradation through autophagy requires peroxisomal division. Autophagy. 2014;10:652-61 pubmed publisher
    ..The segregation of peroxisomes is mediated by 2 dynamin-related GTPases, Dnm1 and Vps1, whereas, the degradation of peroxisomes is accomplished through pexophagy, a selective type of autophagy...
  25. Koirala S, Guo Q, Kalia R, Bui H, Eckert D, Frost A, et al. Interchangeable adaptors regulate mitochondrial dynamin assembly for membrane scission. Proc Natl Acad Sci U S A. 2013;110:E1342-51 pubmed publisher
    Mitochondrial fission is mediated by the dynamin-related GTPases Dnm1/Drp1 (yeast/mammals), which form spirals around constricted sites on mitochondria...
  26. Itoh K, Tamura Y, Iijima M, Sesaki H. Effects of Fcj1-Mos1 and mitochondrial division on aggregation of mitochondrial DNA nucleoids and organelle morphology. Mol Biol Cell. 2013;24:1842-51 pubmed publisher
    ..Moreover, connecting mitochondria by deleting the DNM1 gene required for organelle division enhances aggregation of mtDNA nucleoids in fcj1 and mos1 cells, whereas single ..
  27. Palermo V, Falcone C, Mazzoni C. Apoptosis and aging in mitochondrial morphology mutants of S. cerevisiae. Folia Microbiol (Praha). 2007;52:479-83 pubmed
    ..MDM30 and DNM1, the genes encoding an F-box protein and the dynamin-related GTPase, respectively, are involved in triggering aging ..
  28. Li Y, Chen G, Liu W. Alterations in the interaction between GAL4 and GAL80 effect regulation of the yeast GAL regulon mediated by the F box protein Dsg1. Curr Microbiol. 2010;61:210-6 pubmed publisher
    ..affected by the absence of dsg1 and the induction defect with deletion of dsg1 was partly rescued by disruption of dnm1, a gene encoding a component of the mitochondria division machinery...
  29. Bui H, Karren M, Bhar D, Shaw J. A novel motif in the yeast mitochondrial dynamin Dnm1 is essential for adaptor binding and membrane recruitment. J Cell Biol. 2012;199:613-22 pubmed publisher
    ..Using yeast as a model, we show that the Insert B domain of the Dnm1 guanosine triphosphatase (a DRP) contains a novel motif required for association with the mitochondrial adaptor ..
  30. Mao K, Wang K, Liu X, Klionsky D. The scaffold protein Atg11 recruits fission machinery to drive selective mitochondria degradation by autophagy. Dev Cell. 2013;26:9-18 pubmed publisher
    ..is induced, the fission complex is recruited to the degrading mitochondria through an interaction between Atg11 and Dnm1; interfering with this interaction severely blocks mitophagy...
  31. Fernández Cid A, Riera A, Herrero P, Moreno F. Glucose levels regulate the nucleo-mitochondrial distribution of Mig2. Mitochondrion. 2012;12:370-80 pubmed publisher
    ..antagonistically with respect to the fission-promoting components, because mitochondrial aggregation induced by DNM1 deletion was rescued in the ?dnm1?mig2 double mutant...
  32. Scheckhuber C, Erjavec N, Tinazli A, Hamann A, Nystrom T, Osiewacz H. Reducing mitochondrial fission results in increased life span and fitness of two fungal ageing models. Nat Cell Biol. 2007;9:99-105 pubmed
    ..Deletion of the Dnm1 gene delays the transformation of filamentous to punctuate mitochondria and retards ageing without impairing ..
  33. Wells R, Picton L, Williams S, Tan F, Hill R. Direct binding of the dynamin-like GTPase, Dnm1, to mitochondrial dynamics protein Fis1 is negatively regulated by the Fis1 N-terminal arm. J Biol Chem. 2007;282:33769-75 pubmed
    Recruitment of a dynamin-like GTPase (Drp1/Dlp1/Dnm1) to membranes requires the mitochondrial dynamics protein Fis1. Mdv1 has been proposed to act as an adaptor between Fis1 and Dnm1 in Saccharomyces cerevisiae. We show that S...
  34. Tar K, Dange T, Yang C, Yao Y, Bulteau A, Salcedo E, et al. Proteasomes associated with the Blm10 activator protein antagonize mitochondrial fission through degradation of the fission protein Dnm1. J Biol Chem. 2014;289:12145-56 pubmed publisher
    ..The degradation of Dnm1, the main factor mediating mitochondrial fission, is impaired in the absence of BLM10 in vitro and in vivo...
  35. Würtz C, Schliebs W, Erdmann R, Rottensteiner H. Dynamin-like protein-dependent formation of Woronin bodies in Saccharomyces cerevisiae upon heterologous expression of a single protein. FEBS J. 2008;275:2932-41 pubmed publisher
    ..The data indicate that Woronin bodies emerge after the formation of a HEX1 core crystal within peroxisomes followed by Vps1p- and Dnm1p-mediated fission. ..
  36. Lefevre S, Sliwa D, Rustin P, Camadro J, Santos R. Oxidative stress induces mitochondrial fragmentation in frataxin-deficient cells. Biochem Biophys Res Commun. 2012;418:336-41 pubmed publisher
    ..Here we present evidence that in yeast frataxin-deficiency interferes with mitochondrial dynamics, which might therefore be relevant for the pathophysiology of FA. ..
  37. Bernhardt D, Müller M, Reichert A, Osiewacz H. Simultaneous impairment of mitochondrial fission and fusion reduces mitophagy and shortens replicative lifespan. Sci Rep. 2015;5:7885 pubmed publisher
    ..deletion mutant of Saccharomyces cerevisiae in which two essential components of the fission and fusion machinery, Dnm1 and Mgm1, are simultaneously ablated, contain wild-type like filamentous mitochondria, but are characterized by ..
  38. Hammermeister M, Schödel K, Westermann B. Mdm36 is a mitochondrial fission-promoting protein in Saccharomyces cerevisiae. Mol Biol Cell. 2010;21:2443-52 pubmed publisher
    The division of mitochondrial membranes is a complex process mediated by the dynamin-related protein Dnm1 in yeast, acting in concert with several cofactors...
  39. Joshi A, Thompson M, Fei N, Huttemann M, Greenberg M. Cardiolipin and mitochondrial phosphatidylethanolamine have overlapping functions in mitochondrial fusion in Saccharomyces cerevisiae. J Biol Chem. 2012;287:17589-97 pubmed publisher
    ..Deletion of DNM1, required for mitochondrial fission, restored the tubular mitochondrial morphology...