DNF2

Summary

Gene Symbol: DNF2
Description: aminophospholipid-translocating P4-type ATPase DNF2
Alias: aminophospholipid-translocating P4-type ATPase DNF2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Furuta N, Fujimura Kamada K, Saito K, Yamamoto T, Tanaka K. Endocytic recycling in yeast is regulated by putative phospholipid translocases and the Ypt31p/32p-Rcy1p pathway. Mol Biol Cell. 2007;18:295-312 pubmed
    ..We propose that the Ypt31p/32p-Rcy1p pathway regulates putative phospholipid translocases to promote formation of vesicles destined for the trans-Golgi network from early endosomes. ..
  2. Iwamoto K, Kobayashi S, Fukuda R, Umeda M, Kobayashi T, Ohta A. Local exposure of phosphatidylethanolamine on the yeast plasma membrane is implicated in cell polarity. Genes Cells. 2004;9:891-903 pubmed
    ..Furthermore, S. cerevisiae ros3, dnf1 and dnf2 null mutants, which were known to be defective in the internalization of fluorescence-labelled PE, as well as the ..
  3. Rockwell N, Wolfger H, Kuchler K, Thorner J. ABC transporter Pdr10 regulates the membrane microenvironment of Pdr12 in Saccharomyces cerevisiae. J Membr Biol. 2009;229:27-52 pubmed publisher
    ..leaflet asymmetry requires P-type ATPases, which are proposed to act as inward-directed lipid translocases (Dnf1, Dnf2, and the associated protein Lem3), and ATP-binding cassette (ABC) transporters, which are proposed to act as ..
  4. Hua Z, Fatheddin P, Graham T. An essential subfamily of Drs2p-related P-type ATPases is required for protein trafficking between Golgi complex and endosomal/vacuolar system. Mol Biol Cell. 2002;13:3162-77 pubmed
    ..translocases (APTs): DRS2, NEO1, and three uncharacterized open reading frames that we have named DNF1, DNF2, and DNF3 for DRS2/NEO1 family...
  5. Riekhof W, Wu J, Gij├│n M, Zarini S, Murphy R, Voelker D. Lysophosphatidylcholine metabolism in Saccharomyces cerevisiae: the role of P-type ATPases in transport and a broad specificity acyltransferase in acylation. J Biol Chem. 2007;282:36853-61 pubmed
    ..These results establish a new pathway for the net synthesis of PtdCho in yeast and provide new tools for the study of PtdCho synthesis, transport, and remodeling...
  6. Pomorski T, Lombardi R, Riezman H, Devaux P, van Meer G, Holthuis J. Drs2p-related P-type ATPases Dnf1p and Dnf2p are required for phospholipid translocation across the yeast plasma membrane and serve a role in endocytosis. Mol Biol Cell. 2003;14:1240-54 pubmed
    ..Moreover, it appears that Dnf1p, Dnf2p and Drs2p each help regulate the transbilayer lipid arrangement in the plasma membrane, and that this regulation is critical for budding endocytic vesicles...
  7. Stevens H, Malone L, Nichols J. The putative aminophospholipid translocases, DNF1 and DNF2, are not required for 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylserine flip across the plasma membrane of Saccharomyces cerevisiae. J Biol Chem. 2008;283:35060-9 pubmed publisher
    ..In strains in which DNF1 and DNF2 are both deleted, the flip of NBD-PS is increased approximately 2-fold over that of the isogenic parent strain, ..
  8. Noji T, Yamamoto T, Saito K, Fujimura Kamada K, Kondo S, Tanaka K. Mutational analysis of the Lem3p-Dnf1p putative phospholipid-translocating P-type ATPase reveals novel regulatory roles for Lem3p and a carboxyl-terminal region of Dnf1p independent of the phospholipid-translocating activity of Dnf1p in yeast. Biochem Biophys Res Commun. 2006;344:323-31 pubmed
    ..Our results suggest that the Lem3p-Dnf1p complex may play a role distinct from its plasma membrane APLT activity when it substitutes for the Cdc50p-Drs2p complex, its redundant partner in the endosomal/trans-Golgi network compartments...
  9. Das A, Slaughter B, Unruh J, Bradford W, Alexander R, Rubinstein B, et al. Flippase-mediated phospholipid asymmetry promotes fast Cdc42 recycling in dynamic maintenance of cell polarity. Nat Cell Biol. 2012;14:304-10 pubmed publisher
    ..Here we find that a lipid flippase complex, composed of Lem3, Dnf1 or Dnf2, has a role in the dynamic recycling of the Cdc42 GTPase, a key regulator of cell polarity, in yeast...

More Information

Publications17

  1. Niles B, Joslin A, Fresques T, Powers T. TOR complex 2-Ypk1 signaling maintains sphingolipid homeostasis by sensing and regulating ROS accumulation. Cell Rep. 2014;6:541-52 pubmed publisher
    ..Thus, ROS sensing and signaling by TORC2/Ypk1 play a central physiological role in sphingolipid biosynthesis and in the maintenance of cell growth and viability. ..
  2. Hachiro T, Yamamoto T, Nakano K, Tanaka K. Phospholipid flippases Lem3p-Dnf1p and Lem3p-Dnf2p are involved in the sorting of the tryptophan permease Tat2p in yeast. J Biol Chem. 2013;288:3594-608 pubmed publisher
  3. Niles B, Powers T. TOR complex 2-Ypk1 signaling regulates actin polarization via reactive oxygen species. Mol Biol Cell. 2014;25:3962-72 pubmed publisher
  4. Baldridge R, Graham T. Identification of residues defining phospholipid flippase substrate specificity of type IV P-type ATPases. Proc Natl Acad Sci U S A. 2012;109:E290-8 pubmed publisher
    ..Modeling of residues involved in substrate selection suggests a novel P-type ATPase transport pathway at the protein/lipid interface and a potential solution to the giant substrate problem. ..
  5. Stone A, Chau C, Eaton C, Foran E, Kapur M, Prevatt E, et al. Biochemical characterization of P4-ATPase mutations identified in patients with progressive familial intrahepatic cholestasis. J Biol Chem. 2012;287:41139-51 pubmed publisher
    ..The results of these measurements have implications for the basis of the disease and for the mechanism of phospholipid transit through the enzyme during the reaction cycle...
  6. Riekhof W, Voelker D. Uptake and utilization of lyso-phosphatidylethanolamine by Saccharomyces cerevisiae. J Biol Chem. 2006;281:36588-96 pubmed
  7. Sartorel E, Barrey E, Lau R, Thorner J. Plasma membrane aminoglycerolipid flippase function is required for signaling competence in the yeast mating pheromone response pathway. Mol Biol Cell. 2015;26:134-50 pubmed publisher
    ..In Saccharomyces cerevisiae, five related gene products (Dnf1, Dnf2, Dnf3, Drs2, and Neo1) are implicated in flipping of phosphatidylethanolamine, phosphatidylserine, and ..
  8. Roelants F, Su B, von Wulffen J, Ramachandran S, Sartorel E, TROTT A, et al. Protein kinase Gin4 negatively regulates flippase function and controls plasma membrane asymmetry. J Cell Biol. 2015;208:299-311 pubmed publisher
    ..Two of the P-type ATPases (flippases) in yeast, Dnf1 and Dnf2, translocate aminoglycerophospholipids from the outer to the inner leaflet, stimulated via phosphorylation by ..