DNF1

Summary

Gene Symbol: DNF1
Description: aminophospholipid-translocating P4-type ATPase DNF1
Alias: aminophospholipid-translocating P4-type ATPase DNF1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Iwamoto K, Kobayashi S, Fukuda R, Umeda M, Kobayashi T, Ohta A. Local exposure of phosphatidylethanolamine on the yeast plasma membrane is implicated in cell polarity. Genes Cells. 2004;9:891-903 pubmed
    ..Furthermore, S. cerevisiae ros3, dnf1 and dnf2 null mutants, which were known to be defective in the internalization of fluorescence-labelled PE, as well ..
  2. Stevens H, Malone L, Nichols J. The putative aminophospholipid translocases, DNF1 and DNF2, are not required for 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylserine flip across the plasma membrane of Saccharomyces cerevisiae. J Biol Chem. 2008;283:35060-9 pubmed publisher
    ..In strains in which DNF1 and DNF2 are both deleted, the flip of NBD-PS is increased approximately 2-fold over that of the isogenic parent ..
  3. Baldridge R, Graham T. Two-gate mechanism for phospholipid selection and transport by type IV P-type ATPases. Proc Natl Acad Sci U S A. 2013;110:E358-67 pubmed publisher
    ..Here, through a combination of genetic screening and directed mutagenesis with the type IV P-type ATPases Dnf1 and Drs2 from budding yeast, we identify more than a dozen residues that determine headgroup specificity for ..
  4. Hachiro T, Yamamoto T, Nakano K, Tanaka K. Phospholipid flippases Lem3p-Dnf1p and Lem3p-Dnf2p are involved in the sorting of the tryptophan permease Tat2p in yeast. J Biol Chem. 2013;288:3594-608 pubmed publisher
    ..Here, we report that Lem3p-Dnf1/2p are involved in transport of the tryptophan permease Tat2p to the plasma membrane...
  5. Rockwell N, Wolfger H, Kuchler K, Thorner J. ABC transporter Pdr10 regulates the membrane microenvironment of Pdr12 in Saccharomyces cerevisiae. J Membr Biol. 2009;229:27-52 pubmed publisher
    ..of leaflet asymmetry requires P-type ATPases, which are proposed to act as inward-directed lipid translocases (Dnf1, Dnf2, and the associated protein Lem3), and ATP-binding cassette (ABC) transporters, which are proposed to act as ..
  6. Hua Z, Fatheddin P, Graham T. An essential subfamily of Drs2p-related P-type ATPases is required for protein trafficking between Golgi complex and endosomal/vacuolar system. Mol Biol Cell. 2002;13:3162-77 pubmed
    ..aminophospholipid translocases (APTs): DRS2, NEO1, and three uncharacterized open reading frames that we have named DNF1, DNF2, and DNF3 for DRS2/NEO1 family...
  7. Pomorski T, Lombardi R, Riezman H, Devaux P, van Meer G, Holthuis J. Drs2p-related P-type ATPases Dnf1p and Dnf2p are required for phospholipid translocation across the yeast plasma membrane and serve a role in endocytosis. Mol Biol Cell. 2003;14:1240-54 pubmed
    ..Moreover, it appears that Dnf1p, Dnf2p and Drs2p each help regulate the transbilayer lipid arrangement in the plasma membrane, and that this regulation is critical for budding endocytic vesicles...
  8. Baldridge R, Graham T. Identification of residues defining phospholipid flippase substrate specificity of type IV P-type ATPases. Proc Natl Acad Sci U S A. 2012;109:E290-8 pubmed publisher
    ..Here, we identify residues that confer differences in substrate specificity between Drs2 and Dnf1, Saccharomyces cerevisiae P4-ATPases that preferentially flip PS and phosphatidylcholine (PC), respectively...
  9. Noji T, Yamamoto T, Saito K, Fujimura Kamada K, Kondo S, Tanaka K. Mutational analysis of the Lem3p-Dnf1p putative phospholipid-translocating P-type ATPase reveals novel regulatory roles for Lem3p and a carboxyl-terminal region of Dnf1p independent of the phospholipid-translocating activity of Dnf1p in yeast. Biochem Biophys Res Commun. 2006;344:323-31 pubmed
    ..Our results suggest that the Lem3p-Dnf1p complex may play a role distinct from its plasma membrane APLT activity when it substitutes for the Cdc50p-Drs2p complex, its redundant partner in the endosomal/trans-Golgi network compartments...

More Information

Publications29

  1. Takeda M, Yamagami K, Tanaka K. Role of phosphatidylserine in phospholipid flippase-mediated vesicle transport in Saccharomyces cerevisiae. Eukaryot Cell. 2014;13:363-75 pubmed publisher
    ..These results suggest that flippase-dependent vesicle formation is mediated by phospholipid flipping, not by flipped phospholipids. ..
  2. Furuta N, Fujimura Kamada K, Saito K, Yamamoto T, Tanaka K. Endocytic recycling in yeast is regulated by putative phospholipid translocases and the Ypt31p/32p-Rcy1p pathway. Mol Biol Cell. 2007;18:295-312 pubmed
    ..We propose that the Ypt31p/32p-Rcy1p pathway regulates putative phospholipid translocases to promote formation of vesicles destined for the trans-Golgi network from early endosomes. ..
  3. Riekhof W, Wu J, Gijón M, Zarini S, Murphy R, Voelker D. Lysophosphatidylcholine metabolism in Saccharomyces cerevisiae: the role of P-type ATPases in transport and a broad specificity acyltransferase in acylation. J Biol Chem. 2007;282:36853-61 pubmed
    ..These results establish a new pathway for the net synthesis of PtdCho in yeast and provide new tools for the study of PtdCho synthesis, transport, and remodeling...
  4. Liu K, Hua Z, Nepute J, Graham T. Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway. Mol Biol Cell. 2007;18:487-500 pubmed
    ..These results demonstrate an essential role for endocytosis in retrieving proteins back to the Golgi, and they define critical cargos of the NPFXD/Sla1p system...
  5. Niles B, Joslin A, Fresques T, Powers T. TOR complex 2-Ypk1 signaling maintains sphingolipid homeostasis by sensing and regulating ROS accumulation. Cell Rep. 2014;6:541-52 pubmed publisher
    ..Thus, ROS sensing and signaling by TORC2/Ypk1 play a central physiological role in sphingolipid biosynthesis and in the maintenance of cell growth and viability. ..
  6. Saito K, Fujimura Kamada K, Furuta N, Kato U, Umeda M, Tanaka K. Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae. Mol Biol Cell. 2004;15:3418-32 pubmed
    ..Thus, phospholipid asymmetry plays an important role in the establishment of cell polarity; the Cdc50p/Lem3p family likely constitute potential subunits specific to unique P-type ATPases of the APT subfamily...
  7. Baldridge R, Xu P, Graham T. Type IV P-type ATPases distinguish mono- versus diacyl phosphatidylserine using a cytofacial exit gate in the membrane domain. J Biol Chem. 2013;288:19516-27 pubmed publisher
    ..Preferred translocation substrates for the budding yeast P4-ATPases Dnf1 and Dnf2 include lysophosphatidylcholine, lysophosphatidylethanolamine, derivatives of phosphatidylcholine and ..
  8. Puts C, Panatala R, Hennrich H, Tsareva A, Williamson P, Holthuis J. Mapping functional interactions in a heterodimeric phospholipid pump. J Biol Chem. 2012;287:30529-40 pubmed publisher
    ..Collectively, our data indicate that a dynamic association between subunit and transporter is crucial for the transport reaction cycle of the heterodimer. ..
  9. Das A, Slaughter B, Unruh J, Bradford W, Alexander R, Rubinstein B, et al. Flippase-mediated phospholipid asymmetry promotes fast Cdc42 recycling in dynamic maintenance of cell polarity. Nat Cell Biol. 2012;14:304-10 pubmed publisher
    ..Here we find that a lipid flippase complex, composed of Lem3, Dnf1 or Dnf2, has a role in the dynamic recycling of the Cdc42 GTPase, a key regulator of cell polarity, in yeast...
  10. Catty P, de Kerchove d Exaerde A, Goffeau A. The complete inventory of the yeast Saccharomyces cerevisiae P-type transport ATPases. FEBS Lett. 1997;409:325-32 pubmed
    ..It is proposed that the latter family which has homologs in Tetrahymena thermophila, Plasmodium falciparum and Caenorhabditis elegans constitutes a new group called P4-ATPases with characteristic topology and aminoacid signatures. ..
  11. Sartorel E, Barrey E, Lau R, Thorner J. Plasma membrane aminoglycerolipid flippase function is required for signaling competence in the yeast mating pheromone response pathway. Mol Biol Cell. 2015;26:134-50 pubmed publisher
    ..In Saccharomyces cerevisiae, five related gene products (Dnf1, Dnf2, Dnf3, Drs2, and Neo1) are implicated in flipping of phosphatidylethanolamine, phosphatidylserine, and ..
  12. Niles B, Powers T. TOR complex 2-Ypk1 signaling regulates actin polarization via reactive oxygen species. Mol Biol Cell. 2014;25:3962-72 pubmed publisher
  13. Yamagami K, Yamamoto T, Sakai S, Mioka T, Sano T, Igarashi Y, et al. Inositol depletion restores vesicle transport in yeast phospholipid flippase mutants. PLoS ONE. 2015;10:e0120108 pubmed publisher
    ..These results suggest that inositol depletion changes the lipid composition of the endosomal/TGN membranes, which results in vesicle formation from these membranes in the absence of flippases. ..
  14. Roland B, Graham T. Directed evolution of a sphingomyelin flippase reveals mechanism of substrate backbone discrimination by a P4-ATPase. Proc Natl Acad Sci U S A. 2016;113:E4460-6 pubmed publisher
    ..screen in the yeast Saccharomyces cerevisiae has identified several gain-of-function mutations in the P4-ATPase Dnf1 that facilitate the transport of a novel lipid substrate, sphingomyelin...
  15. Roelants F, Su B, von Wulffen J, Ramachandran S, Sartorel E, TROTT A, et al. Protein kinase Gin4 negatively regulates flippase function and controls plasma membrane asymmetry. J Cell Biol. 2015;208:299-311 pubmed publisher
    ..Two of the P-type ATPases (flippases) in yeast, Dnf1 and Dnf2, translocate aminoglycerophospholipids from the outer to the inner leaflet, stimulated via phosphorylation ..
  16. Benito B, Garciadeblas B, Rodríguez Navarro A. Potassium- or sodium-efflux ATPase, a key enzyme in the evolution of fungi. Microbiology. 2002;148:933-41 pubmed
    ..The parasites Leishmania and Trypanosoma have ATPases phylogenetically related to fungal K(+)-ATPases, which are probably functional homologues of the fungal enzymes. ..
  17. Muthusamy B, Raychaudhuri S, Natarajan P, Abe F, Liu K, Prinz W, et al. Control of protein and sterol trafficking by antagonistic activities of a type IV P-type ATPase and oxysterol binding protein homologue. Mol Biol Cell. 2009;20:2920-31 pubmed publisher
  18. Riekhof W, Voelker D. Uptake and utilization of lyso-phosphatidylethanolamine by Saccharomyces cerevisiae. J Biol Chem. 2006;281:36588-96 pubmed
  19. Xu P, Baldridge R, Chi R, Burd C, Graham T. Phosphatidylserine flipping enhances membrane curvature and negative charge required for vesicular transport. J Cell Biol. 2013;202:875-86 pubmed publisher
    ..Using mutant forms of Drs2 and the related protein Dnf1, which alter their ability to recognize phosphatidylserine, we show that translocation of this substrate to the ..
  20. Puts C, Lenoir G, Krijgsveld J, Williamson P, Holthuis J. A P4-ATPase protein interaction network reveals a link between aminophospholipid transport and phosphoinositide metabolism. J Proteome Res. 2010;9:833-42 pubmed publisher
    ..Together, these findings suggest that aminophospholipid transport and phosphoinositide metabolism are interconnected at the Golgi. ..