DNA2

Summary

Gene Symbol: DNA2
Description: bifunctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2
Alias: WEB2, bifunctional ATP-dependent DNA helicase/ssDNA endodeoxyribonuclease DNA2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Budd M, Campbell J. A yeast gene required for DNA replication encodes a protein with homology to DNA helicases. Proc Natl Acad Sci U S A. 1995;92:7642-6 pubmed
    ..RNA synthesis is normal in the mutant but DNA synthesis ceases upon shift to the nonpermissive temperature. The DNA2 gene was cloned by complementation of the dna2ts gene phenotype. The gene is essential for viability...
  2. Fiorentino D, Crabtree G. Characterization of Saccharomyces cerevisiae dna2 mutants suggests a role for the helicase late in S phase. Mol Biol Cell. 1997;8:2519-37 pubmed
    ..This gene is identical to DNA2, encoding a helicase required for DNA replication...
  3. Bae S, Choi E, Lee K, Park J, Lee S, Seo Y. Dna2 of Saccharomyces cerevisiae possesses a single-stranded DNA-specific endonuclease activity that is able to act on double-stranded DNA in the presence of ATP. J Biol Chem. 1998;273:26880-90 pubmed
    To gain further insights into the biological functions of Dna2, previously known as a cellular replicative helicase in Saccharomyces cerevisiae, we examined biochemical properties of the recombinant Dna2 protein purified to homogeneity...
  4. Budd M, Campbell J. The pattern of sensitivity of yeast dna2 mutants to DNA damaging agents suggests a role in DSB and postreplication repair pathways. Mutat Res. 2000;459:173-86 pubmed
    The Saccharomyces cerevisiae DNA2 gene encodes a DNA-stimulated ATPase and DNA helicase/nuclease essential for DNA replication...
  5. Lee C, Shin Y, Phung T, Bae J, Kang Y, Nguyen T, et al. Involvement of Vts1, a structure-specific RNA-binding protein, in Okazaki fragment processing in yeast. Nucleic Acids Res. 2010;38:1583-95 pubmed publisher
    ..In this study, we identified VTS1 as a multi-copy suppressor of dna2-K1080E, a lethal mutant allele of DNA2 that lacks DNA helicase activity...
  6. Jin Y, Ayyagari R, Resnick M, Gordenin D, Burgers P. Okazaki fragment maturation in yeast. II. Cooperation between the polymerase and 3'-5'-exonuclease activities of Pol delta in the creation of a ligatable nick. J Biol Chem. 2003;278:1626-33 pubmed
    ..Maturation was efficient in the absence of Dna2 and required Dna2 only when FEN1 activity was compromised...
  7. Budd M, Campbell J. A yeast replicative helicase, Dna2 helicase, interacts with yeast FEN-1 nuclease in carrying out its essential function. Mol Cell Biol. 1997;17:2136-42 pubmed
    We have recently described a new helicase, the Dna2 helicase, that is essential for yeast DNA replication. We now show that the yeast FEN-1 (yFEN-1) nuclease interacts genetically and biochemically with Dna2 helicase...
  8. Kang Y, Kang M, Kim J, Lee C, Cho I, Hurwitz J, et al. The MPH1 gene of Saccharomyces cerevisiae functions in Okazaki fragment processing. J Biol Chem. 2009;284:10376-86 pubmed publisher
    ..Purified Mph1 stimulated the endonuclease activities of both Fen1 and Dna2, which act faithfully in the processing of Okazaki fragments...
  9. Niu H, Chung W, Zhu Z, Kwon Y, Zhao W, Chi P, et al. Mechanism of the ATP-dependent DNA end-resection machinery from Saccharomyces cerevisiae. Nature. 2010;467:108-11 pubmed publisher
    ..machinery comprising the Saccharomyces cerevisiae Mre11-Rad50-Xrs2 (MRX) complex, the Sgs1-Top3-Rmi1 complex, Dna2 protein and the heterotrimeric ssDNA-binding protein RPA...

More Information

Publications71

  1. Bonetti D, Martina M, Clerici M, Lucchini G, Longhese M. Multiple pathways regulate 3' overhang generation at S. cerevisiae telomeres. Mol Cell. 2009;35:70-81 pubmed publisher
    ..Moreover, Dna2 might also support Sgs1 activity, as it acts redundantly with Exo1, but not with Sgs1...
  2. Kim J, Kang Y, Kang H, Kim D, Ryu G, Kang M, et al. In vivo and in vitro studies of Mgs1 suggest a link between genome instability and Okazaki fragment processing. Nucleic Acids Res. 2005;33:6137-50 pubmed
    ..In this study, we identified MGS1 as a multicopy suppressor of the temperature-sensitive dna2Delta405N mutation, a DNA2 allele lacking the N-terminal 405 amino acid residues...
  3. Weitao T, Budd M, Campbell J. Evidence that yeast SGS1, DNA2, SRS2, and FOB1 interact to maintain rDNA stability. Mutat Res. 2003;532:157-72 pubmed
    ..Both pausing and breakage are elevated in the hypomorphic dna2-2 helicase mutant. Deletion of FOB1 suppresses the elevated pausing and DSB formation...
  4. Cejka P, Cannavo E, Polaczek P, Masuda Sasa T, Pokharel S, Campbell J, et al. DNA end resection by Dna2-Sgs1-RPA and its stimulation by Top3-Rmi1 and Mre11-Rad50-Xrs2. Nature. 2010;467:112-6 pubmed publisher
    ..Here we biochemically reconstitute elements of the resection process and reveal that it requires the nuclease Dna2, the RecQ-family helicase Sgs1 and the ssDNA-binding protein replication protein-A (RPA)...
  5. Kosugi S, Hasebe M, Tomita M, Yanagawa H. Systematic identification of cell cycle-dependent yeast nucleocytoplasmic shuttling proteins by prediction of composite motifs. Proc Natl Acad Sci U S A. 2009;106:10171-6 pubmed publisher
    ..identified all previously reported and 5 previously uncharacterized yeast proteins (Yen1, Psy4, Pds1, Msa1, and Dna2) displaying CDK1- and cell cycle-regulated nuclear transport...
  6. Zhu Z, Chung W, Shim E, Lee S, Ira G. Sgs1 helicase and two nucleases Dna2 and Exo1 resect DNA double-strand break ends. Cell. 2008;134:981-94 pubmed publisher
    ..We show that the Mre11-Rad50-Xrs2 complex (MRX) initiates 5' degradation, whereas Sgs1 and Dna2 degrade 5' strands exposing long 3' strands...
  7. Budd M, Reis C, Smith S, Myung K, Campbell J. Evidence suggesting that Pif1 helicase functions in DNA replication with the Dna2 helicase/nuclease and DNA polymerase delta. Mol Cell Biol. 2006;26:2490-500 pubmed
    ..plays a wider role in DNA replication than previously appreciated and that it likely functions in conjunction with Dna2 helicase/nuclease as a component of the OFP machinery...
  8. Weitao T, Budd M, Hoopes L, Campbell J. Dna2 helicase/nuclease causes replicative fork stalling and double-strand breaks in the ribosomal DNA of Saccharomyces cerevisiae. J Biol Chem. 2003;278:22513-22 pubmed
    ..recombination and chromosome instability in Saccharomyces cerevisiae and contributes to the shortened lifespan of dna2 mutants...
  9. Masuda Sasa T, Polaczek P, Campbell J. Single strand annealing and ATP-independent strand exchange activities of yeast and human DNA2: possible role in Okazaki fragment maturation. J Biol Chem. 2006;281:38555-64 pubmed
    The Dna2 protein is a multifunctional enzyme with 5'-3' DNA helicase, DNA-dependent ATPase, 3' exo/endonuclease, and 5' exo/endonuclease...
  10. Budd M, Choe W, Campbell J. DNA2 encodes a DNA helicase essential for replication of eukaryotic chromosomes. J Biol Chem. 1995;270:26766-9 pubmed
    ..S. A. 30, 6465-6469; Budd, M. E., and Campbell, J. L. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 7642-7646). The DNA2 gene is essential and encodes a 172-kDa protein with DNA helicase motifs in its C-terminal half and an N-terminal ..
  11. Parenteau J, Wellinger R. Accumulation of single-stranded DNA and destabilization of telomeric repeats in yeast mutant strains carrying a deletion of RAD27. Mol Cell Biol. 1999;19:4143-52 pubmed
    ..Our data thus provide in vivo evidence for the types of DNA lesions predicted to occur when lagging-strand synthesis is deficient and suggest that Dna2p and Rad27p collaborate in the processing of Okazaki fragments. ..
  12. Stewart J, Campbell J, Bambara R. Significance of the dissociation of Dna2 by flap endonuclease 1 to Okazaki fragment processing in Saccharomyces cerevisiae. J Biol Chem. 2009;284:8283-91 pubmed publisher
    ..Flap endonuclease 1 (FEN1) and Dna2 are responsible for flap cleavage...
  13. Bae K, Kim H, Bae S, Kang H, Brill S, Seo Y. Bimodal interaction between replication-protein A and Dna2 is critical for Dna2 function both in vivo and in vitro. Nucleic Acids Res. 2003;31:3006-15 pubmed
    ..plays a role in Okazaki fragment processing by acting as a molecular switch between the two endonucleases, Dna2 and Fen1, to ensure the complete removal of primer RNAs in Saccharomyces cerevisiae...
  14. Formosa T, Nittis T. Dna2 mutants reveal interactions with Dna polymerase alpha and Ctf4, a Pol alpha accessory factor, and show that full Dna2 helicase activity is not essential for growth. Genetics. 1999;151:1459-70 pubmed
    Mutations in the gene for the conserved, essential nuclease-helicase Dna2 from the yeast Saccharomyces cerevisiae were found to interact genetically with POL1 and CTF4, which encode a DNA Polymerase alpha subunit and an associated protein,..
  15. Balakrishnan L, Polaczek P, Pokharel S, Campbell J, Bambara R. Dna2 exhibits a unique strand end-dependent helicase function. J Biol Chem. 2010;285:38861-8 pubmed publisher
    b>Dna2 endonuclease/helicase participates in eukaryotic DNA transactions including cleavage of long flaps generated during Okazaki fragment processing...
  16. Chen X, Cui D, Papusha A, Zhang X, Chu C, Tang J, et al. The Fun30 nucleosome remodeller promotes resection of DNA double-strand break ends. Nature. 2012;489:576-80 pubmed publisher
    ..have been described both in cells and in vitro, one relying on Exo1 exonuclease and the other on Sgs1 helicase and Dna2 nuclease...
  17. Budd M, Campbell J. Interplay of Mre11 nuclease with Dna2 plus Sgs1 in Rad51-dependent recombinational repair. PLoS ONE. 2009;4:e4267 pubmed publisher
    ..One candidate nuclease that has not been tested to date because it is encoded by an essential gene is the Dna2 helicase/nuclease. We recently reported the ability to suppress the lethality of a dna2Delta with a pif1Delta...
  18. Pike J, Burgers P, Campbell J, Bambara R. Pif1 helicase lengthens some Okazaki fragment flaps necessitating Dna2 nuclease/helicase action in the two-nuclease processing pathway. J Biol Chem. 2009;284:25170-80 pubmed publisher
    ..An alternative pathway involving FEN1 and the nuclease/helicase Dna2 has been proposed for flaps that become long enough to bind replication protein A (RPA)...
  19. Shim E, Chung W, Nicolette M, Zhang Y, Davis M, Zhu Z, et al. Saccharomyces cerevisiae Mre11/Rad50/Xrs2 and Ku proteins regulate association of Exo1 and Dna2 with DNA breaks. EMBO J. 2010;29:3370-80 pubmed publisher
    ..Mre11/Rad50/Xrs2 (MRX) and Sae2 are implicated in the onset of 5'-strand resection, whereas Sgs1/Top3/Rmi1 with Dna2 and Exo1 are involved in extensive resection...
  20. Chen X, Niu H, Chung W, Zhu Z, Papusha A, Shim E, et al. Cell cycle regulation of DNA double-strand break end resection by Cdk1-dependent Dna2 phosphorylation. Nat Struct Mol Biol. 2011;18:1015-9 pubmed publisher
    ..The Mre11-Rad50-Xrs2 complex with Sae2 initiates resection, whereas two nucleases, Exo1 and Dna2, and the DNA helicase-topoisomerase complex Sgs1-Top3-Rmi1 generate longer ssDNA at DSBs...
  21. Kao H, Campbell J, Bambara R. Dna2p helicase/nuclease is a tracking protein, like FEN1, for flap cleavage during Okazaki fragment maturation. J Biol Chem. 2004;279:50840-9 pubmed
    ..We propose that the two nucleases both track, Dna2p first and then FEN1, to remove initiator RNA via long flap intermediates. ..
  22. Masuda Sasa T, Polaczek P, Peng X, Chen L, Campbell J. Processing of G4 DNA by Dna2 helicase/nuclease and replication protein A (RPA) provides insights into the mechanism of Dna2/RPA substrate recognition. J Biol Chem. 2008;283:24359-73 pubmed publisher
    ..The lagging strand DNA replication protein, Dna2, has demonstrated a unique localization to telomeres and a role in de novo telomere biogenesis, prompting us to ..
  23. Hoopes L, Budd M, Choe W, Weitao T, Campbell J. Mutations in DNA replication genes reduce yeast life span. Mol Cell Biol. 2002;22:4136-46 pubmed
    ..We show that a replicative yeast helicase/nuclease, encoded by DNA2 and a member of the same helicase subfamily as the RecQ helicases, is required for normal life span...
  24. Borges V, Smith D, Whitehouse I, Uhlmann F. An Eco1-independent sister chromatid cohesion establishment pathway in S. cerevisiae. Chromosoma. 2013;122:121-34 pubmed publisher
    ..Thus, Ctf4 and Chl1 delineate an additional acetylation-independent pathway that might hold important clues as to the mechanism of sister chromatid cohesion establishment...
  25. Choe W, Budd M, Imamura O, Hoopes L, Campbell J. Dynamic localization of an Okazaki fragment processing protein suggests a novel role in telomere replication. Mol Cell Biol. 2002;22:4202-17 pubmed
    We have found that the Dna2 helicase-nuclease, thought to be involved in maturation of Okazaki fragments, is a component of telomeric chromatin...
  26. Meurisse J, Bacquin A, Richet N, Charbonnier J, Ochsenbein F, Peyroche A. Hug1 is an intrinsically disordered protein that inhibits ribonucleotide reductase activity by directly binding Rnr2 subunit. Nucleic Acids Res. 2014;42:13174-85 pubmed publisher
    ..Hence, both the regulation and the mode of action of Hug1 are different from those of the small protein inhibitors Dif1 and Sml1, and Hug1 can be considered as a regulator for fine-tuning of RNR activity. ..
  27. Braguglia D, Heun P, Pasero P, Duncker B, Gasser S. Semi-conservative replication in yeast nuclear extracts requires Dna2 helicase and supercoiled template. J Mol Biol. 1998;281:631-49 pubmed
    ..Using this novel system we demonstrate that semi-conservative replication, but not polymerase activity per se, requires the activity of the DNA helicase encoded by DNA2.
  28. Schlesinger M, Formosa T. POB3 is required for both transcription and replication in the yeast Saccharomyces cerevisiae. Genetics. 2000;155:1593-606 pubmed
    ..were observed between pob3 mutations and the genes encoding several DNA replication factors, including POL1, CTF4, DNA2, and CHL12...
  29. Wittmeyer J, Formosa T. The Saccharomyces cerevisiae DNA polymerase alpha catalytic subunit interacts with Cdc68/Spt16 and with Pob3, a protein similar to an HMG1-like protein. Mol Cell Biol. 1997;17:4178-90 pubmed
    ..Based on these results and because Cdc68 has been implicated as a regulator of chromatin structure, we postulate that polymerase alpha may interact with these proteins to gain access to its template or to origins of replication in vivo. ..
  30. Lee M, Lee C, Demin A, Munashingha P, Amangyeld T, Kwon B, et al. Rad52/Rad59-dependent recombination as a means to rectify faulty Okazaki fragment processing. J Biol Chem. 2014;289:15064-79 pubmed publisher
    The correct removal of 5'-flap structures by Rad27 and Dna2 during Okazaki fragment maturation is crucial for the stable maintenance of genetic materials and cell viability...
  31. Lee C, Lee M, Kang H, Kim D, Kang Y, Bae S, et al. The N-terminal 45-kDa domain of Dna2 endonuclease/helicase targets the enzyme to secondary structure DNA. J Biol Chem. 2013;288:9468-81 pubmed publisher
    ..become long enough to bind replication protein A or form hairpin structures, the helicase/endonuclease enzyme, Dna2, becomes critical because of its ability to remove replication protein A-coated or secondary structure flaps...
  32. Chen H, Lisby M, Symington L. RPA coordinates DNA end resection and prevents formation of DNA hairpins. Mol Cell. 2013;50:589-600 pubmed publisher
    ..participates in DNA double-strand break repair by stimulating 5'-3' end resection by the Sgs1/BLM helicase and Dna2 endonuclease in vitro...
  33. Munashingha P, Lee C, Kang Y, Shin Y, Nguyen T, Seo Y. The trans-autostimulatory activity of Rad27 suppresses dna2 defects in Okazaki fragment processing. J Biol Chem. 2012;287:8675-87 pubmed publisher
    b>Dna2 and Rad27 (yeast Fen1) are the two endonucleases critical for Okazaki fragment processing during lagging strand DNA synthesis that have been shown to interact genetically and physically...
  34. Saugar I, Parker J, Zhao S, Ulrich H. The genome maintenance factor Mgs1 is targeted to sites of replication stress by ubiquitylated PCNA. Nucleic Acids Res. 2012;40:245-57 pubmed publisher
    ..Our identification of Mgs1 as a UBZ-dependent downstream effector of ubiquitylated PCNA suggests an explanation for the ambivalent role of the protein in damage processing. ..
  35. Kumar S, Burgers P. Lagging strand maturation factor Dna2 is a component of the replication checkpoint initiation machinery. Genes Dev. 2013;27:313-21 pubmed publisher
    ..Saccharomyces cerevisiae Dna2, a nuclease-helicase that is essential for Okazaki fragment maturation, is employed specifically during S phase to ..
  36. Ireland M, Reinke S, Livingston D. The impact of lagging strand replication mutations on the stability of CAG repeat tracts in yeast. Genetics. 2000;155:1657-65 pubmed
    ..Mutations in a DNA helicase/deoxyribonuclease (dna2-1) or in two RNase H activities (rnh1Delta and rnh35Delta) do not have a significant effect on CAG repeat tract ..
  37. Ferrari M, Dibitetto D, De Gregorio G, Eapen V, Rawal C, Lazzaro F, et al. Functional interplay between the 53BP1-ortholog Rad9 and the Mre11 complex regulates resection, end-tethering and repair of a double-strand break. PLoS Genet. 2015;11:e1004928 pubmed publisher
    ..stranded DNA filaments can be exposed from a DSB through the redundant activities of the Exo1 nuclease and the Dna2 nuclease with the Sgs1 helicase...
  38. Gómez González B, Felipe Abrio I, Aguilera A. The S-phase checkpoint is required to respond to R-loops accumulated in THO mutants. Mol Cell Biol. 2009;29:5203-13 pubmed publisher
    ..In light of these results, we propose a model in which R-loop-mediated recombination is explained by template switching. ..
  39. Kanellis P, Agyei R, Durocher D. Elg1 forms an alternative PCNA-interacting RFC complex required to maintain genome stability. Curr Biol. 2003;13:1583-95 pubmed
    ..Collectively, these results reveal that Elg1 forms a novel and conserved alternative RFC complex. Furthermore, we propose that genome instability arises at high frequency in elg1 mutants due to a defect in Okazaki fragment maturation. ..
  40. Stewart J, Miller A, Campbell J, Bambara R. Dynamic removal of replication protein A by Dna2 facilitates primer cleavage during Okazaki fragment processing in Saccharomyces cerevisiae. J Biol Chem. 2008;283:31356-65 pubmed publisher
    ..Polymerase delta displaces the primer into a flap for processing. Dna2 nuclease/helicase and flap endonuclease 1 (FEN1) are proposed to cleave the flap...
  41. Stith C, Sterling J, Resnick M, Gordenin D, Burgers P. Flexibility of eukaryotic Okazaki fragment maturation through regulated strand displacement synthesis. J Biol Chem. 2008;283:34129-40 pubmed publisher
    ..exonuclease activity of Pol delta. This core maturation machinery can be assisted by Dna2 nuclease/helicase that processes long flaps...
  42. Rossi M, Pike J, Wang W, Burgers P, Campbell J, Bambara R. Pif1 helicase directs eukaryotic Okazaki fragments toward the two-nuclease cleavage pathway for primer removal. J Biol Chem. 2008;283:27483-93 pubmed publisher
    ..of flaps escapes cleavage and grows long enough to be coated with RPA and requires the consecutive action of the Dna2 and FEN1 nucleases for processing. Here we tested whether RPA inhibits FEN1 cleavage of long flaps as proposed...
  43. Bastos de Oliveira F, Kim D, Cussiol J, Das J, Jeong M, Doerfler L, et al. Phosphoproteomics reveals distinct modes of Mec1/ATR signaling during DNA replication. Mol Cell. 2015;57:1124-1132 pubmed publisher
    ..This "replication-correlated" mode of Mec1 action requires the 9-1-1 clamp and the Dna2 lagging-strand factor and is distinguishable from Mec1's action in activating the downstream kinase Rad53...
  44. Gobbini E, Villa M, Gnugnoli M, Menin L, Clerici M, Longhese M. Sae2 Function at DNA Double-Strand Breaks Is Bypassed by Dampening Tel1 or Rad53 Activity. PLoS Genet. 2015;11:e1005685 pubmed publisher
    ..that suppress both the DNA damage hypersensitivity and the resection defect of sae2Δ cells through an Sgs1-Dna2-dependent mechanism. These suppression events do not involve escaping the checkpoint-mediated cell cycle arrest...
  45. Demin A, Lee M, Lee C, Seo Y. GSK-3? Homolog Rim11 and the Histone Deacetylase Complex Ume6-Sin3-Rpd3 Are Involved in Replication Stress Response Caused by Defects in Dna2. Genetics. 2017;206:829-842 pubmed publisher
    ..We attempted to identify novel factors that are required in the absence of the helicase activity of Dna2, an essential enzyme in Okazaki-fragment maturation...
  46. Formosa T, Eriksson P, Wittmeyer J, Ginn J, Yu Y, Stillman D. Spt16-Pob3 and the HMG protein Nhp6 combine to form the nucleosome-binding factor SPN. EMBO J. 2001;20:3506-17 pubmed
    ..These complexes have altered electrophoretic mobility and a distinct pattern of enhanced sensitivity to DNase I. These results suggest that Spt16-Pob3 and Nhp6 cooperate to function as a novel nucleosome reorganizing factor. ..
  47. Bhattacharyya S, Lahue R. Srs2 helicase of Saccharomyces cerevisiae selectively unwinds triplet repeat DNA. J Biol Chem. 2005;280:33311-7 pubmed
    ..Overall, the selective unwinding activity of Srs2 on triplet repeat hairpin DNA helps explain the genetic evidence that Srs2, not the RecQ homolog Sgs1, is a preferred helicase for preventing expansions. ..
  48. Ang J, Duffy S, Segovia R, Stirling P, Hieter P. Dosage Mutator Genes in Saccharomyces cerevisiae: A Novel Mutator Mode-of-Action of the Mph1 DNA Helicase. Genetics. 2016;204:975-986 pubmed publisher
    ..single gene, and characterized the five strongest mutators, MPH1 (mutator phenotype 1), RRM3, UBP12, PIF1, and DNA2 We show that, for MPH1, the yeast homolog of Fanconi Anemia complementation group M (FANCM), the overexpression ..
  49. Wanrooij P, Tannous E, Kumar S, Navadgi Patil V, Burgers P. Probing the Mec1ATR Checkpoint Activation Mechanism with Small Peptides. J Biol Chem. 2016;291:393-401 pubmed publisher
    ..pombe Cut5), and the multifunctional nuclease/helicase Dna2. Here, we use small peptides to determine the requirements for Mec1 activation...
  50. Levikova M, Cejka P. The Saccharomyces cerevisiae Dna2 can function as a sole nuclease in the processing of Okazaki fragments in DNA replication. Nucleic Acids Res. 2015;43:7888-97 pubmed publisher
    ..Previously, Dna2 was implicated to function upstream of flap endonuclease 1 (Fen1 or Rad27) in the processing of long flaps bound by ..
  51. Manfrini N, Guerini I, Citterio A, Lucchini G, Longhese M. Processing of meiotic DNA double strand breaks requires cyclin-dependent kinase and multiple nucleases. J Biol Chem. 2010;285:11628-37 pubmed publisher
    ..Furthermore, the helicase Sgs1 and the nucleases Exo1 and Dna2 participate in lengthening the 5'-3' resection tracts during meiosis by controlling a step subsequent to Spo11 ..
  52. Yimit A, Riffle M, Brown G. Genetic Regulation of Dna2 Localization During the DNA Damage Response. G3 (Bethesda). 2015;5:1937-44 pubmed publisher
    DNA damage response pathways are crucial for protecting genome stability in all eukaryotes. Saccharomyces cerevisiae Dna2 has both helicase and nuclease activities that are essential for Okazaki fragment maturation, and Dna2 is involved ..
  53. Miller A, Daley J, Pham N, Niu H, Xue X, Ira G, et al. A novel role of the Dna2 translocase function in DNA break resection. Genes Dev. 2017;31:503-510 pubmed publisher
    ..b>Dna2, a flap endonuclease with 5'-3' helicase activity, is involved in the resection process...
  54. Levikova M, Klaue D, Seidel R, Cejka P. Nuclease activity of Saccharomyces cerevisiae Dna2 inhibits its potent DNA helicase activity. Proc Natl Acad Sci U S A. 2013;110:E1992-2001 pubmed publisher
    b>Dna2 is a nuclease-helicase involved in several key pathways of eukaryotic DNA metabolism. The potent nuclease activity of Saccharomyces cerevisiae Dna2 was reported to be required for all its in vivo functions tested to date...
  55. Henry R, Balakrishnan L, Ying Lin S, Campbell J, Bambara R. Components of the secondary pathway stimulate the primary pathway of eukaryotic Okazaki fragment processing. J Biol Chem. 2010;285:28496-505 pubmed publisher
    ..The flaps must then be cleaved by Dna2 nuclease/helicase before FEN1 can act. Pif1 helicase aids creation of long flaps...
  56. Kang M, Lee C, Kang Y, Cho I, Nguyen T, Seo Y. Genetic and functional interactions between Mus81-Mms4 and Rad27. Nucleic Acids Res. 2010;38:7611-25 pubmed publisher
    The two endonucleases, Rad27 (yeast Fen1) and Dna2, jointly participate in the processing of Okazaki fragments in yeasts...
  57. Budd M, Campbell J. Dna2 is involved in CA strand resection and nascent lagging strand completion at native yeast telomeres. J Biol Chem. 2013;288:29414-29 pubmed publisher
    ..At short de novo telomeres generated artificially by HO endonuclease in the G2 phase, we show that dna2-defective strains are impaired in both telomere elongation and sequential 5'-CA resection...
  58. Bonetti D, Villa M, Gobbini E, Cassani C, Tedeschi G, Longhese M. Escape of Sgs1 from Rad9 inhibition reduces the requirement for Sae2 and functional MRX in DNA end resection. EMBO Rep. 2015;16:351-61 pubmed publisher
    ..are involved in the onset of DSB resection, whereas extensive resection requires Exo1 and the concerted action of Dna2 and Sgs1...
  59. Villa F, Simon A, Ortiz Bazan M, Kilkenny M, Wirthensohn D, Wightman M, et al. Ctf4 Is a Hub in the Eukaryotic Replisome that Links Multiple CIP-Box Proteins to the CMG Helicase. Mol Cell. 2016;63:385-96 pubmed publisher
    ..Mutations in the CIP-box motifs of the Dna2 nuclease or the rDNA-associated protein Tof2 do not perturb DNA synthesis genome-wide, but instead lead to a ..
  60. Budd M, Antoshechkin I, Reis C, Wold B, Campbell J. Inviability of a DNA2 deletion mutant is due to the DNA damage checkpoint. Cell Cycle. 2011;10:1690-8 pubmed
    b>Dna2 is a dual polarity exo/endonuclease, and 5' to 3' DNA helicase involved in Okazaki Fragment Processing (OFP) and Double-Strand Break (DSB) Repair. In yeast, DNA2 is an essential gene, as expected for a DNA replication protein...
  61. Colosio A, Frattini C, Pellicanò G, Villa Hernández S, Bermejo R. Nucleolytic processing of aberrant replication intermediates by an Exo1-Dna2-Sae2 axis counteracts fork collapse-driven chromosome instability. Nucleic Acids Res. 2016;44:10676-10690 pubmed
    ..Genetic analyses demonstrated a functional interplay of Exo1 with Mus81, Dna2 and Sae2 nucleases in promoting cell survival following replication stress, suggestive of concerted nucleolytic ..
  62. Pokharel S, Campbell J. Cross talk between the nuclease and helicase activities of Dna2: role of an essential iron-sulfur cluster domain. Nucleic Acids Res. 2012;40:7821-30 pubmed publisher
    b>Dna2 nuclease/helicase is a multitasking protein involved in DNA replication and recombinational repair, and it is important for preservation of genomic stability...