DMC1

Summary

Gene Symbol: DMC1
Description: recombinase DMC1
Alias: ISC2, recombinase DMC1
Species: Saccharomyces cerevisiae S288c
Products:     DMC1

Top Publications

  1. Shinohara A, Gasior S, Ogawa T, Kleckner N, Bishop D. Saccharomyces cerevisiae recA homologues RAD51 and DMC1 have both distinct and overlapping roles in meiotic recombination. Genes Cells. 1997;2:615-29 pubmed
    Rad51 and Dmc1 are Saccharomyces cerevisiae homologues of the Escherichia coli recombination protein RecA...
  2. San Segundo P, Roeder G. Pch2 links chromatin silencing to meiotic checkpoint control. Cell. 1999;97:313-24 pubmed
    ..Mutation of PCH2 relieves the checkpoint-induced pachytene arrest of the zip1, zip2, and dmc1 mutants, resulting in chromosome missegregation and low spore viability...
  3. Hong E, Shinohara A, Bishop D. Saccharomyces cerevisiae Dmc1 protein promotes renaturation of single-strand DNA (ssDNA) and assimilation of ssDNA into homologous super-coiled duplex DNA. J Biol Chem. 2001;276:41906-12 pubmed
    b>Dmc1 and Rad51 are eukaryotic RecA homologues that are involved in meiotic recombination. The expression of Dmc1 is limited to meiosis, whereas Rad51 is expressed in mitosis and meiosis...
  4. Iacovella M, Daly C, Kelly J, Michielsen A, Clyne R. Analysis of Polo-like kinase Cdc5 in the meiosis recombination checkpoint. Cell Cycle. 2010;9:1182-93 pubmed
    ..The cdc5-ad mutation that renders cells unable to adapt to DNA damage in mitosis did not affect checkpoint adaptation in meiosis, indicating that the mechanisms of checkpoint adaptation in mitosis and meiosis are not fully conserved. ..
  5. Cloud V, Chan Y, Grubb J, Budke B, Bishop D. Rad51 is an accessory factor for Dmc1-mediated joint molecule formation during meiosis. Science. 2012;337:1222-5 pubmed publisher
    Meiotic recombination in budding yeast requires two RecA-related proteins, Rad51 and Dmc1, both of which form filaments on DNA capable of directing homology search and catalyzing formation of homologous joint molecules (JMs) and strand ..
  6. Nimonkar A, Dombrowski C, Siino J, Stasiak A, Stasiak A, Kowalczykowski S. Saccharomyces cerevisiae Dmc1 and Rad51 proteins preferentially function with Tid1 and Rad54 proteins, respectively, to promote DNA strand invasion during genetic recombination. J Biol Chem. 2012;287:28727-37 pubmed publisher
    The Saccharomyces cerevisiae Dmc1 and Tid1 proteins are required for the pairing of homologous chromosomes during meiotic recombination...
  7. Holzen T, Shah P, Olivares H, Bishop D. Tid1/Rdh54 promotes dissociation of Dmc1 from nonrecombinogenic sites on meiotic chromatin. Genes Dev. 2006;20:2593-604 pubmed
    The meiosis-specific recombinase Dmc1 plays a critical role in DNA strand exchange in budding yeast. Tid1/Rdh54, a member of the Swi2/Snf2 family of DNA translocases, has been shown to stimulate Dmc1-dependent recombination...
  8. Bishop D, Nikolski Y, Oshiro J, Chon J, Shinohara M, Chen X. High copy number suppression of the meiotic arrest caused by a dmc1 mutation: REC114 imposes an early recombination block and RAD54 promotes a DMC1-independent DSB repair pathway. Genes Cells. 1999;4:425-44 pubmed
    b>DMC1, the meiosis-specific eukaryotic homologue of bacterial recA, is required for completion of meiotic recombination and cell cycle progression past prophase...
  9. Zierhut C, Berlinger M, Rupp C, Shinohara A, Klein F. Mnd1 is required for meiotic interhomolog repair. Curr Biol. 2004;14:752-62 pubmed
    ..We propose a model in which Mnd1 facilitates chromatin accessibility, which is required to allow strand invasion in meiotic chromatin. ..

More Information

Publications59

  1. Xu L, Weiner B, Kleckner N. Meiotic cells monitor the status of the interhomolog recombination complex. Genes Dev. 1997;11:106-18 pubmed
    ..g., pachytene). In yeast, mutations of this type include rad50S, dmc1, rad51, and zip1. Rad50 is likely part of a recombination initiation complex...
  2. Zanders S, Sonntag Brown M, Chen C, Alani E. Pch2 modulates chromatid partner choice during meiotic double-strand break repair in Saccharomyces cerevisiae. Genetics. 2011;188:511-21 pubmed publisher
    ..These defects appear most pronounced in dmc1? mutants, which are defective for interhomolog repair, and explain the previously reported observation that pch2? ..
  3. Wang Y, Chang C, Wu J, Tung K. Nuclear localization of the meiosis-specific transcription factor Ndt80 is regulated by the pachytene checkpoint. Mol Biol Cell. 2011;22:1878-86 pubmed publisher
    ..The NDT80-bc mutation relieves the checkpoint-mediated arrest of the zip1, dmc1, and hop2 mutants, producing spores with low viability...
  4. Dresser M, Ewing D, Conrad M, Dominguez A, Barstead R, Jiang H, et al. DMC1 functions in a Saccharomyces cerevisiae meiotic pathway that is largely independent of the RAD51 pathway. Genetics. 1997;147:533-44 pubmed
    ..A screen for dominant meiotic mutants provided DMC1-G126D, a dominant allele mutated in the conserved ATP-binding site (specifically, the A-loop motif) that confers a ..
  5. San Segundo P, Roeder G. Role for the silencing protein Dot1 in meiotic checkpoint control. Mol Biol Cell. 2000;11:3601-15 pubmed
    ..silencing protein Dot1 (also known as Pch1) is required for checkpoint-mediated pachytene arrest of the zip1 and dmc1 mutants of Saccharomyces cerevisiae...
  6. Lao J, Oh S, Shinohara M, Shinohara A, Hunter N. Rad52 promotes postinvasion steps of meiotic double-strand-break repair. Mol Cell. 2008;29:517-24 pubmed publisher
    ..The meiosis-specific RecA-homolog Dmc1 does not show this dependence, however...
  7. Grushcow J, Holzen T, Park K, Weinert T, Lichten M, Bishop D. Saccharomyces cerevisiae checkpoint genes MEC1, RAD17 and RAD24 are required for normal meiotic recombination partner choice. Genetics. 1999;153:607-20 pubmed
    ..gene function prevents meiotic progression when recombination is blocked by mutations in the recA homologue DMC1. Bypass of dmc1 arrest by mutation of the DNA damage checkpoint genes MEC1, RAD17, or RAD24 results in a dramatic ..
  8. Niu H, Wan L, Baumgartner B, Schaefer D, Loidl J, Hollingsworth N. Partner choice during meiosis is regulated by Hop1-promoted dimerization of Mek1. Mol Biol Cell. 2005;16:5804-18 pubmed
    ..The C domain is needed for spore viability, for chromosome synapsis, and for preventing DMC1-independent DSB repair, indicating it plays a role in the BSCR...
  9. Callender T, Hollingsworth N. Mek1 suppression of meiotic double-strand break repair is specific to sister chromatids, chromosome autonomous and independent of Rec8 cohesin complexes. Genetics. 2010;185:771-82 pubmed publisher
    ..is determined at the time of strand invasion and is mediated by two recombinases: Rad51 and the meiosis-specific Dmc1. In budding yeast, interhomolog bias is created in part by the activity of a meiosis-specific kinase, Mek1, which ..
  10. Hepworth S, Friesen H, Segall J. NDT80 and the meiotic recombination checkpoint regulate expression of middle sporulation-specific genes in Saccharomyces cerevisiae. Mol Cell Biol. 1998;18:5750-61 pubmed
    ..In contrast, middle sporulation genes were not expressed in a dmc1 strain, which fails to enter the meiotic divisions because a defect in meiotic recombination leads to a RAD17-..
  11. Pak J, Segall J. Role of Ndt80, Sum1, and Swe1 as targets of the meiotic recombination checkpoint that control exit from pachytene and spore formation in Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:6430-40 pubmed
    ..effects of manipulating the three known targets of the meiotic recombination checkpoint, NDT80, SWE1, and SUM1, in dmc1-arrested cells...
  12. Busygina V, Gaines W, Xu Y, Kwon Y, Williams G, Lin S, et al. Functional attributes of the Saccharomyces cerevisiae meiotic recombinase Dmc1. DNA Repair (Amst). 2013;12:707-12 pubmed publisher
    The role of Dmc1 as a meiosis-specific general recombinase was first demonstrated in Saccharomyces cerevisiae. Progress in understanding the biochemical mechanism of ScDmc1 has been hampered by its tendency to form inactive aggregates...
  13. Tsubouchi H, Roeder G. The importance of genetic recombination for fidelity of chromosome pairing in meiosis. Dev Cell. 2003;5:915-25 pubmed
    ..Genetic analysis indicates that Hop2 acts in the same pathway as the Rad51 and Dmc1 proteins, two homologs of E. coli RecA...
  14. Bishop D, Park D, Xu L, Kleckner N. DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression. Cell. 1992;69:439-56 pubmed
    b>DMC1 is a new meiosis-specific yeast gene. Dmc1 protein is structurally similar to bacterial RecA proteins...
  15. Tsubouchi H, Roeder G. Budding yeast Hed1 down-regulates the mitotic recombination machinery when meiotic recombination is impaired. Genes Dev. 2006;20:1766-75 pubmed
    In budding yeast, there are two RecA homologs: Rad51 and Dmc1. While Rad51 is involved in both mitotic and meiotic recombination, Dmc1 participates specifically in meiotic recombination...
  16. Bishop D. RecA homologs Dmc1 and Rad51 interact to form multiple nuclear complexes prior to meiotic chromosome synapsis. Cell. 1994;79:1081-92 pubmed
    b>Dmc1 and Rad51, yeast homologs of the E. coli RecA protein, are shown by immunostaining to localize to as many as 64 sites within spread meiotic nuclei...
  17. Schwacha A, Kleckner N. Interhomolog bias during meiotic recombination: meiotic functions promote a highly differentiated interhomolog-only pathway. Cell. 1997;90:1123-35 pubmed
    ..and between sisters in yeast strains lacking the meiotic chromosomal protein Red1, the meiotic recA homolog Dmc1, and/or mitotic recA homolog(s), Rad51, Rad55, and Rad57...
  18. Chang Y, Lo Y, Lee M, Leng C, Hu S, Chang C, et al. Molecular visualization of the yeast Dmc1 protein ring and Dmc1-ssDNA nucleoprotein complex. Biochemistry. 2005;44:6052-8 pubmed
    Saccharomyces cerevisiae Dmc1, a meiosis-specific homologue of RecA, catalyzes homologous pairing and strand exchange during meiotic DNA recombination...
  19. Lee M, Chang Y, Hong E, Grubb J, Chang C, Bishop D, et al. Calcium ion promotes yeast Dmc1 activity via formation of long and fine helical filaments with single-stranded DNA. J Biol Chem. 2005;280:40980-4 pubmed
    b>Dmc1 is specifically required for homologous recombination during meiosis...
  20. Thompson D, Stahl F. Genetic control of recombination partner preference in yeast meiosis. Isolation and characterization of mutants elevated for meiotic unequal sister-chromatid recombination. Genetics. 1999;153:621-41 pubmed
    ..The genes RED1, MEK1, RAD24, RAD17, and MEC1 are required for meiotic prophase arrest induced by a dmc1 mutation, which defines a meiotic recombination checkpoint. Meiotic unequal SCR was also elevated in a rad17 mutant...
  21. Henry J, Camahort R, Rice D, Florens L, Swanson S, Washburn M, et al. Mnd1/Hop2 facilitates Dmc1-dependent interhomolog crossover formation in meiosis of budding yeast. Mol Cell Biol. 2006;26:2913-23 pubmed
    ..Recombination in meiosis in Saccharomyces cerevisiae relies on two Escherichia coli recA homologs, Rad51 and Dmc1, as well as the more recently discovered heterodimer Mnd1/Hop2. Meiotic recombination in S...
  22. Leu J, Roeder G. The pachytene checkpoint in S. cerevisiae depends on Swe1-mediated phosphorylation of the cyclin-dependent kinase Cdc28. Mol Cell. 1999;4:805-14 pubmed
    ..The requirement for Swe1 distinguishes the pachytene checkpoint from the DNA damage checkpoints operating in vegetative cells. ..
  23. Eichinger C, Jentsch S. Synaptonemal complex formation and meiotic checkpoint signaling are linked to the lateral element protein Red1. Proc Natl Acad Sci U S A. 2010;107:11370-5 pubmed publisher
    ..Thus, Red1, in addition to its structural role in the SC, is a crucial coordinator of meiosis by coupling checkpoint signaling to SC formation...
  24. Rockmill B, Sym M, Scherthan H, Roeder G. Roles for two RecA homologs in promoting meiotic chromosome synapsis. Genes Dev. 1995;9:2684-95 pubmed
    Previous studies have shown that the RAD51 and DMC1 genes of Saccharomyces cerevisiae encode homologs of the Escherichia coli RecA strand exchange enzyme...
  25. Ho H, Burgess S. Pch2 acts through Xrs2 and Tel1/ATM to modulate interhomolog bias and checkpoint function during meiosis. PLoS Genet. 2011;7:e1002351 pubmed publisher
    ..In addition, Xrs2, like Pch2, is required for checkpoint-mediated delay conferred by the failure to synapse chromosomes...
  26. Lao J, Cloud V, Huang C, Grubb J, Thacker D, Lee C, et al. Meiotic crossover control by concerted action of Rad51-Dmc1 in homolog template bias and robust homeostatic regulation. PLoS Genet. 2013;9:e1003978 pubmed publisher
    ..Two DNA strand-exchange proteins, Rad51 and Dmc1, are required for meiotic recombination in many organisms...
  27. McKee A, Kleckner N. A general method for identifying recessive diploid-specific mutations in Saccharomyces cerevisiae, its application to the isolation of mutants blocked at intermediate stages of meiotic prophase and characterization of a new gene SAE2. Genetics. 1997;146:797-816 pubmed
    ..Identified in this screen were null mutations of the DMC1 gene, nonnull mutations of RAD50 (rad50S), and mutations in three new genes designed SAE1, SAE2 and SAE3 (..
  28. Sawarynski K, Najor N, Kepsel A, Brush G. Sic1-induced DNA rereplication during meiosis. Proc Natl Acad Sci U S A. 2009;106:232-7 pubmed publisher
    ..Deletion of DMC1 abolished DNA rereplication, but additional deletion of RAD17 restored the original phenotype...
  29. Refolio E, Cavero S, Marcon E, Freire R, San Segundo P. The Ddc2/ATRIP checkpoint protein monitors meiotic recombination intermediates. J Cell Sci. 2011;124:2488-500 pubmed publisher
    ..Thus, our results point to an evolutionary conserved role for Ddc2/ATRIP in monitoring meiotic chromosome metabolism. ..
  30. Chan Y, Brown M, Qin D, Handa N, Bishop D. The third exon of the budding yeast meiotic recombination gene HOP2 is required for calcium-dependent and recombinase Dmc1-specific stimulation of homologous strand assimilation. J Biol Chem. 2014;289:18076-86 pubmed publisher
    ..open reading frame to direct synthesis of wild type Hop2 protein, we show that the Hop2-Mnd1 heterodimer stimulated Dmc1 D-loop activity up to 30-fold, similar to the activity of mammalian Hop2-Mnd1...
  31. Pohl T, Nickoloff J. Rad51-independent interchromosomal double-strand break repair by gene conversion requires Rad52 but not Rad55, Rad57, or Dmc1. Mol Cell Biol. 2008;28:897-906 pubmed
    ..A Rad51 homolog, Dmc1, is important for meiotic HR...
  32. Shinohara M, Sakai K, Shinohara A, Bishop D. Crossover interference in Saccharomyces cerevisiae requires a TID1/RDH54- and DMC1-dependent pathway. Genetics. 2003;163:1273-86 pubmed
    Two RecA-like recombinases, Rad51 and Dmc1, function together during double-strand break (DSB)-mediated meiotic recombination to promote homologous strand invasion in the budding yeast Saccharomyces cerevisiae...
  33. Katis V, Lipp J, Imre R, Bogdanova A, Okaz E, Habermann B, et al. Rec8 phosphorylation by casein kinase 1 and Cdc7-Dbf4 kinase regulates cohesin cleavage by separase during meiosis. Dev Cell. 2010;18:397-409 pubmed publisher
    ..Our data suggest that PP2A protects centromeric cohesion by opposing CK1delta/epsilon- and DDK-dependent phosphorylation of Rec8. ..
  34. Ferrari S, Grubb J, Bishop D. The Mei5-Sae3 protein complex mediates Dmc1 activity in Saccharomyces cerevisiae. J Biol Chem. 2009;284:11766-70 pubmed publisher
    ..DNA and relieves the inhibition of the strand assimilation and DNA binding abilities of the meiotic recombinase Dmc1 imposed by the single-stranded DNA-binding protein replication protein A...
  35. Cho H, Kong Y, Hong S, Kim K. Hop2 and Sae3 Are Required for Dmc1-Mediated Double-Strand Break Repair via Homolog Bias during Meiosis. Mol Cells. 2016;39:550-6 pubmed publisher
    ..This genetic exchange process is tightly controlled by the eukaryotic RecA homologs Rad51 and Dmc1, which are involved in strand exchange of meiotic recombination, with Rad51 participating specifically in mitotic ..
  36. Tishkoff D, Rockmill B, Roeder G, Kolodner R. The sep1 mutant of Saccharomyces cerevisiae arrests in pachytene and is deficient in meiotic recombination. Genetics. 1995;139:495-509 pubmed
    ..In an assay of intrachromosomal pop-out recombination, the sep1, dmc1 and rad51 single mutations had only small effects; however, pop-out recombination was virtually eliminated in the ..
  37. Copsey A, Tang S, Jordan P, Blitzblau H, Newcombe S, Chan A, et al. Smc5/6 coordinates formation and resolution of joint molecules with chromosome morphology to ensure meiotic divisions. PLoS Genet. 2013;9:e1004071 pubmed publisher
  38. Liu Y, Gaines W, Callender T, Busygina V, Oke A, Sung P, et al. Down-regulation of Rad51 activity during meiosis in yeast prevents competition with Dmc1 for repair of double-strand breaks. PLoS Genet. 2014;10:e1004005 pubmed publisher
    ..double strand breaks (DSBs) using sister chromatids, in contrast to the conserved, meiosis-specific recombinase, Dmc1, which preferentially repairs programmed DSBs using homologs...
  39. Bendjilali N, MacLeon S, Kalra G, Willis S, Hossian A, Avery E, et al. Time-Course Analysis of Gene Expression During the Saccharomyces cerevisiae Hypoxic Response. G3 (Bethesda). 2017;7:221-231 pubmed publisher
    ..transcription-quantitative PCR (RT-qPCR) analysis confirmed that the lysine methyltransferase EFM6 and the recombinase DMC1, both conserved in humans, are indeed oxygen-responsive...
  40. Usui T, Kanehara Y. Elevated Rad53 kinase activity influences formation and interhomolog repair of meiotic DNA double-strand breaks in budding yeast. Biochem Biophys Res Commun. 2013;441:593-9 pubmed publisher
    ..The overexpression led to partial activation of Rad53, uncovering that the negative impacts of Rad53 kinase activation on meiotic progression, and formation and interhomolog repair of meiotic programmed DSBs. ..
  41. Hayase A, Takagi M, Miyazaki T, Oshiumi H, Shinohara M, Shinohara A. A protein complex containing Mei5 and Sae3 promotes the assembly of the meiosis-specific RecA homolog Dmc1. Cell. 2004;119:927-40 pubmed
    Meiotic recombination requires the meiosis-specific RecA homolog Dmc1 as well as the mitotic RecA homolog Rad51...
  42. Lee J, Steinfeld J, Qi Z, Kwon Y, Sung P, Greene E. Sequence imperfections and base triplet recognition by the Rad51/RecA family of recombinases. J Biol Chem. 2017;292:11125-11135 pubmed publisher
    ..Here, we examined how base triplet stepping by RecA, Rad51, and Dmc1 is affected by DNA sequence imperfections, such as single and multiple mismatches, abasic sites, and single ..
  43. Callender T, Laureau R, Wan L, Chen X, Sandhu R, Laljee S, et al. Mek1 Down Regulates Rad51 Activity during Yeast Meiosis by Phosphorylation of Hed1. PLoS Genet. 2016;12:e1006226 pubmed publisher
    ..In many organisms, there are two strand exchange proteins, Rad51 and the meiosis-specific Dmc1, required for interhomolog (IH) bias...
  44. Sheridan S, Yu X, Roth R, Heuser J, Sehorn M, Sung P, et al. A comparative analysis of Dmc1 and Rad51 nucleoprotein filaments. Nucleic Acids Res. 2008;36:4057-66 pubmed publisher
    The eukaryotic RecA homologs Rad51 and Dmc1 are essential for strand exchange between homologous chromosomes during meiosis...
  45. Acosta I, Ontoso D, San Segundo P. The budding yeast polo-like kinase Cdc5 regulates the Ndt80 branch of the meiotic recombination checkpoint pathway. Mol Biol Cell. 2011;22:3478-90 pubmed publisher
    ..Our results reveal a role for Cdc5 in meiotic checkpoint control by regulating Ndt80 function. ..
  46. Fukuda T, Ohya Y. Recruitment of RecA homologs Dmc1p and Rad51p to the double-strand break repair site initiated by meiosis-specific endonuclease VDE (PI-SceI). Mol Genet Genomics. 2006;275:204-14 pubmed
  47. Brown M, Grubb J, Zhang A, Rust M, Bishop D. Small Rad51 and Dmc1 Complexes Often Co-occupy Both Ends of a Meiotic DNA Double Strand Break. PLoS Genet. 2015;11:e1005653 pubmed publisher
    The Eukaryotic RecA-like proteins Rad51 and Dmc1 cooperate during meiosis to promote recombination between homologous chromosomes by repairing programmed DNA double strand breaks (DSBs)...
  48. Farmer S, Hong E, Leung W, Argunhan B, Terentyev Y, Humphryes N, et al. Budding yeast Pch2, a widely conserved meiotic protein, is involved in the initiation of meiotic recombination. PLoS ONE. 2012;7:e39724 pubmed publisher
    ..by the pch2 mutation is most prominent in the sae2 mutant background, whereas the impact remains mild in the rad51 dmc1 double mutant background...
  49. Najor N, Weatherford L, Brush G. Prevention of DNA Rereplication Through a Meiotic Recombination Checkpoint Response. G3 (Bethesda). 2016;6:3869-3881 pubmed publisher
    ..When programmed DNA double-strand breaks (DSBs) are generated but not repaired due to absence of DMC1, a pathway involving the checkpoint gene RAD17 prevents this DNA rereplication...
  50. McKee A, Kleckner N. Mutations in Saccharomyces cerevisiae that block meiotic prophase chromosome metabolism and confer cell cycle arrest at pachytene identify two new meiosis-specific genes SAE1 and SAE3. Genetics. 1997;146:817-34 pubmed
    ..The phenotypes conferred by sae3-1 are similar to those conferred by mutation of the yeast RecA homologue DMC1, suggesting that SAE3 and DMC1 act at the same step(s) of chromosome metabolism...