Gene Symbol: DIG2
Description: Dig2p
Alias: RST2, Dig2p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Cook J, Bardwell L, Kron S, Thorner J. Two novel targets of the MAP kinase Kss1 are negative regulators of invasive growth in the yeast Saccharomyces cerevisiae. Genes Dev. 1996;10:2831-48 pubmed
    ..screen for gene products that interact with Kss1, two homologous and previously uncharacterized loci (DIG1 and DIG2, for down-regulator of invasive growth) were identified...
  2. Tedford K, Kim S, Sa D, Stevens K, Tyers M. Regulation of the mating pheromone and invasive growth responses in yeast by two MAP kinase substrates. Curr Biol. 1997;7:228-38 pubmed
    ..Two newly identified proteins, Rst 1 and Rst 2 (also known as Dig1 and Dig2), were found to associate physically with Fus3 and Ste12...
  3. Bardwell L, Cook J, Zhu Shimoni J, Voora D, Thorner J. Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins. Proc Natl Acad Sci U S A. 1998;95:15400-5 pubmed
    ..Herein we show that two nuclear proteins, Dig1 and Dig2, are required cofactors in Kss1-imposed repression...
  4. Breitkreutz A, Boucher L, Tyers M. MAPK specificity in the yeast pheromone response independent of transcriptional activation. Curr Biol. 2001;11:1266-71 pubmed
    ..MAPK specificity in the pheromone response evidently occurs primarily at the substrate level, as opposed to specific kinase activation by dedicated signaling complexes. ..
  5. Chou S, Lane S, Liu H. Regulation of mating and filamentation genes by two distinct Ste12 complexes in Saccharomyces cerevisiae. Mol Cell Biol. 2006;26:4794-805 pubmed
    ..activity is regulated by Fus3 and Kss1 mitogen-activated protein kinases through two Ste12 inhibitors, Dig1 and Dig2. Mating genes are regulated by Ste12 through Ste12 binding sites (pheromone response elements [PREs]), whereas ..
  6. Olson K, Nelson C, Tai G, Hung W, Yong C, Astell C, et al. Two regulators of Ste12p inhibit pheromone-responsive transcription by separate mechanisms. Mol Cell Biol. 2000;20:4199-209 pubmed
    ..Ste12p is negatively regulated by two inhibitor proteins, Dig1p (also called Rst1p) and Dig2p (also called Rst2p)...
  7. Kusari A, Molina D, Sabbagh W, Lau C, Bardwell L. A conserved protein interaction network involving the yeast MAP kinases Fus3 and Kss1. J Cell Biol. 2004;164:267-77 pubmed
    ..of their binding partners, including the Ste7 MAPK kinase, the Ste5 adaptor/scaffold protein, and the Dig1 and Dig2 transcriptional repressors...
  8. Nakano A. Identification and characterization of extragenic suppressors of the yeast sec12 ts mutation. J Biochem. 1996;120:642-6 pubmed
    ..RST1DS is a dominant mutation and causes elevated expression of Sec12p. rst2 and rst3 are recessive and give pleiotropic phenotypes including slow growth at low temperatures and heterogeneous ..
  9. Pi H, Chien C, Fields S. Transcriptional activation upon pheromone stimulation mediated by a small domain of Saccharomyces cerevisiae Ste12p. Mol Cell Biol. 1997;17:6410-8 pubmed
    ..By two-hybrid analysis, this minimal domain interacts with two negative regulators, Dig1p and Dig2p (also designated Rst1p and Rst2p), and the interaction is abolished by mutation of the tyrosines...

More Information


  1. Gelli A. Rst1 and Rst2 are required for the a/alpha diploid cell type in yeast. Mol Microbiol. 2002;46:845-54 pubmed
    ..and the MAP kinase Fus3 phosphorylates two specific repressor proteins Rst1 and Rst2 (also known as Dig1 and Dig2) to promote Ste12-dependent transcription of mating-specific genes...
  2. Breitkreutz A, Boucher L, Breitkreutz B, Sultan M, Jurisica I, Tyers M. Phenotypic and transcriptional plasticity directed by a yeast mitogen-activated protein kinase network. Genetics. 2003;165:997-1015 pubmed
    ..and the transcriptional machinery consists of a network of two MAPKs, Fus3 and Kss1; two regulators, Rst1 and Rst2 (a.k.a. Dig1 and Dig2); and two transcription factors, Ste12 and Tec1...
  3. Blackwell E, Kim H, Stone D. The pheromone-induced nuclear accumulation of the Fus3 MAPK in yeast depends on its phosphorylation state and on Dig1 and Dig2. BMC Cell Biol. 2007;8:44 pubmed
    ..One possibility is that the activation of Fus3 changes its affinity for nuclear and cytoplasmic tethers. Dig1, Dig2, and Ste12 are nuclear proteins that interact with Fus3...
  4. Chen R, Thorner J. Systematic epistasis analysis of the contributions of protein kinase A- and mitogen-activated protein kinase-dependent signaling to nutrient limitation-evoked responses in the yeast Saccharomyces cerevisiae. Genetics. 2010;185:855-70 pubmed publisher
    ..Thus, although there are similarities between haploids and diploids, cell type-specific differences clearly alter the balance of the signaling inputs required to elicit the various nutrient limitation-evoked cellular behaviors. ..
  5. Houser J, Ford E, Nagiec M, Errede B, Elston T. Positive roles for negative regulators in the mating response of yeast. Mol Syst Biol. 2012;8:586 pubmed publisher
    ..First, we found that the transcriptional repressors Dig1 and Dig2 positively affect transcription by stabilizing Ste12...
  6. Ranish J, Yi E, Leslie D, Purvine S, Goodlett D, Eng J, et al. The study of macromolecular complexes by quantitative proteomics. Nat Genet. 2003;33:349-55 pubmed