DID4

Summary

Gene Symbol: DID4
Description: ESCRT-III subunit protein DID4
Alias: CHM2, GRD7, REN1, VPL2, VPS14, VPS2, VPT14, ESCRT-III subunit protein DID4
Species: Saccharomyces cerevisiae S288c
Products:     DID4

Top Publications

  1. Shestakova A, Hanono A, Drosner S, Curtiss M, Davies B, Katzmann D, et al. Assembly of the AAA ATPase Vps4 on ESCRT-III. Mol Biol Cell. 2010;21:1059-71 pubmed publisher
    ..The most significant among these were the binding of Vps4 to Vta1 and to the ESCRT-III subunits Vps2 and Snf7...
  2. Babst M, Katzmann D, Estepa Sabal E, Meerloo T, Emr S. Escrt-III: an endosome-associated heterooligomeric protein complex required for mvb sorting. Dev Cell. 2002;3:271-82 pubmed
    ..The soluble coiled-coil-containing proteins Vps2, Vps20, Vps24, and Snf7 are recruited from the cytoplasm to endosomal membranes where they oligomerize into a ..
  3. Richter C, West M, Odorizzi G. Doa4 function in ILV budding is restricted through its interaction with the Vps20 subunit of ESCRT-III. J Cell Sci. 2013;126:1881-90 pubmed publisher
    ..This rescue in ILV biogenesis requires Doa4 expression but is independent of its Ub hydrolase activity. Thus, binding of Vps20 to the Doa4 N-terminus inhibits a non-catalytic function of Doa4 that promotes ILV formation. ..
  4. Obita T, Saksena S, Ghazi Tabatabai S, Gill D, Perisic O, Emr S, et al. Structural basis for selective recognition of ESCRT-III by the AAA ATPase Vps4. Nature. 2007;449:735-9 pubmed publisher
    ..Here we show that, of the six ESCRT--related subunits in yeast, only Vps2 and Did2 bind the MIT (microtubule interacting and transport) domain of Vps4, and that the carboxy-terminal 30 ..
  5. Azmi I, Davies B, Xiao J, Babst M, Xu Z, Katzmann D. ESCRT-III family members stimulate Vps4 ATPase activity directly or via Vta1. Dev Cell. 2008;14:50-61 pubmed publisher
    ..Two distinct mechanisms of Vps4 stimulation are described: Vps2 can directly stimulate Vps4 via its MIT domain, whereas Vps60 stimulates via Vta1...
  6. Babst M, Katzmann D, Snyder W, Wendland B, Emr S. Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body. Dev Cell. 2002;3:283-9 pubmed
    ..We propose that the ESCRT complexes perform a coordinated cascade of events to select and sort MVB cargoes for delivery to the lumen of the vacuole/lysosome. ..
  7. Rue S, Mattei S, Saksena S, Emr S. Novel Ist1-Did2 complex functions at a late step in multivesicular body sorting. Mol Biol Cell. 2008;19:475-84 pubmed
    ..We provide evidence that the ist1Delta mutation exhibits a synthetic interaction with mutations in VPS2 (DID4) that compromise the Vps2-Vps4 interaction...
  8. Vajjhala P, Nguyen C, Landsberg M, Kistler C, Gan A, King G, et al. The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases. FEBS J. 2008;275:1427-49 pubmed publisher
    ..We propose that Vps4 SRH function requires an intact C-terminal helix. Co-evolution of a distinct Vps4 SRH and C-terminal helix in meiotic clade AAA ATPases supports this possibility. ..
  9. Dimaano C, Jones C, Hanono A, Curtiss M, Babst M. Ist1 regulates Vps4 localization and assembly. Mol Biol Cell. 2008;19:465-74 pubmed
    ..The activity of the MVB pathway might be in part determined by outcome of these two competing activities. ..

More Information

Publications20

  1. Wollert T, Wunder C, Lippincott Schwartz J, Hurley J. Membrane scission by the ESCRT-III complex. Nature. 2009;458:172-7 pubmed publisher
    ..In Saccharomyces cerevisiae, ESCRT-III consists of Vps20, Snf7, Vps24 and Vps2 (also known as Did4), which assemble in that order and require the ATPase Vps4 for their disassembly...
  2. Nothwehr S, Bryant N, Stevens T. The newly identified yeast GRD genes are required for retention of late-Golgi membrane proteins. Mol Cell Biol. 1996;16:2700-7 pubmed
    ..All of the grd mutants exhibited significant mislocalization of A-ALP, as measured by determining the kinetics of A-ALP processing and by analyzing its ..
  3. Palmer S, Smaczynska de Rooij I, Marklew C, Allwood E, Mishra R, Johnson S, et al. A dynamin-actin interaction is required for vesicle scission during endocytosis in yeast. Curr Biol. 2015;25:868-78 pubmed publisher
    ..A model is proposed in which actin filaments bind Vps1 during invagination, and this interaction is important to transduce the force of actin polymerization to the membrane to drive successful scission. ..
  4. Amerik A, Nowak J, Swaminathan S, Hochstrasser M. The Doa4 deubiquitinating enzyme is functionally linked to the vacuolar protein-sorting and endocytic pathways. Mol Biol Cell. 2000;11:3365-80 pubmed
  5. Adell M, Vogel G, Pakdel M, M ller M, Lindner H, Hess M, et al. Coordinated binding of Vps4 to ESCRT-III drives membrane neck constriction during MVB vesicle formation. J Cell Biol. 2014;205:33-49 pubmed publisher
    ..ESCRT-II also initiates the assembly of a ringlike ESCRT-III filament consisting of Vps20, Snf7, Vps24, and Vps2. The AAA-adenosine triphosphatase Vps4 disassembles and recycles the ESCRT-III complex, thereby terminating the ..
  6. Hayashi M, Fukuzawa T, Sorimachi H, Maeda T. Constitutive activation of the pH-responsive Rim101 pathway in yeast mutants defective in late steps of the MVB/ESCRT pathway. Mol Cell Biol. 2005;25:9478-90 pubmed
    ..Three identified suppressors-did4/vps2, vps24, and vps4-all belonged to "class E" vps mutants, which are commonly defective in ..
  7. Bowers K, Lottridge J, Helliwell S, Goldthwaite L, Luzio J, Stevens T. Protein-protein interactions of ESCRT complexes in the yeast Saccharomyces cerevisiae. Traffic. 2004;5:194-210 pubmed
    ..We propose the formation of a large multimeric complex on the endosome membrane consisting of ESCRTI, ESCRTII, ESCRTIII and other associated proteins. ..
  8. Vajjhala P, Catchpoole E, Nguyen C, Kistler C, Munn A. Vps4 regulates a subset of protein interactions at the multivesicular endosome. FEBS J. 2007;274:1894-907 pubmed
    ..We demonstrate that Vps4 interacts directly with Vps2 and Bro1...
  9. Bauer I, Brune T, Preiss R, Kölling R. Evidence for a Nonendosomal Function of the Saccharomyces cerevisiae ESCRT-III-Like Protein Chm7. Genetics. 2015;201:1439-52 pubmed publisher
  10. Peters T, Miller A, Tourette C, Agren H, Hubbard A, Hughes R. Genomic Analysis of ATP Efflux in Saccharomyces cerevisiae. G3 (Bethesda). 2015;6:161-70 pubmed publisher
    ..These results will facilitate analysis of ATP efflux mechanisms in higher eukaryotes. ..
  11. Han H, Monroe N, Votteler J, Shakya B, Sundquist W, Hill C. Binding of Substrates to the Central Pore of the Vps4 ATPase Is Autoinhibited by the Microtubule Interacting and Trafficking (MIT) Domain and Activated by MIT Interacting Motifs (MIMs). J Biol Chem. 2015;290:13490-9 pubmed publisher
    ..These observations support the model that Vps4 substrates are initially recruited by an MIM-MIT interaction that activates the Vps4 central pore to engage substrates and generate force, thereby triggering ESCRT-III disassembly. ..