Gene Symbol: DIA2
Description: DNA-binding SCF ubiquitin ligase subunit DIA2
Alias: YOR29-31, DNA-binding SCF ubiquitin ligase subunit DIA2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Morohashi H, Maculins T, Labib K. The amino-terminal TPR domain of Dia2 tethers SCF(Dia2) to the replisome progression complex. Curr Biol. 2009;19:1943-9 pubmed publisher
    ..The F box protein Dia2 is an important determinant of genome stability in budding yeast [3-5], but its mode of action is poorly understood...
  2. Swaminathan S, Kile A, MacDonald E, Koepp D. Yra1 is required for S phase entry and affects Dia2 binding to replication origins. Mol Cell Biol. 2007;27:4674-84 pubmed
    The Saccharomyces cerevisiae F-box protein Dia2 is important for DNA replication and genomic stability. Using an affinity approach, we identified Yra1, a transcription-coupled mRNA export protein, as a Dia2 interaction partner...
  3. Kus B, Caldon C, Andorn Broza R, Edwards A. Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro. Proteins. 2004;54:455-67 pubmed
    ..Ubc4 thus interacts with multiple SCFs in vitro, and the interactions among SCF and E2 components of the ubiquitination machinery may allow further diversification of the roles of SCFs in vivo. ..
  4. Kile A, Koepp D. Activation of the S-phase checkpoint inhibits degradation of the F-box protein Dia2. Mol Cell Biol. 2010;30:160-71 pubmed publisher
    ..In budding yeast, the chromatin-bound F-box protein Dia2 is required to maintain genomic stability and may help replication complexes overcome sites of damaged DNA and ..
  5. Koepp D, Kile A, Swaminathan S, Rodriguez Rivera V. The F-box protein Dia2 regulates DNA replication. Mol Biol Cell. 2006;17:1540-8 pubmed
    ..b>Dia2 is a Saccharomyces cerevisiae F-box protein previously described to play a role in invasive growth and pheromone ..
  6. Mimura S, Komata M, Kishi T, Shirahige K, Kamura T. SCF(Dia2) regulates DNA replication forks during S-phase in budding yeast. EMBO J. 2009;28:3693-705 pubmed publisher
    b>Dia2 is an F-box protein, which is involved in the regulation of DNA replication in the budding yeast Saccharomyces cerevisiae. The function of Dia2, however, remains largely unknown...
  7. Bairwa N, Mohanty B, Stamenova R, Curcio M, Bastia D. The intra-S phase checkpoint protein Tof1 collaborates with the helicase Rrm3 and the F-box protein Dia2 to maintain genome stability in Saccharomyces cerevisiae. J Biol Chem. 2011;286:2445-54 pubmed publisher
    ..and discovered that Tof1 suppressed excessive retromobility in collaboration with either Rrm3 or the F-box protein Dia2. Although both Rrm3 and Dia2 are believed to facilitate fork movement, fork stalling at DNA-protein complexes did ..
  8. Andress E, Holic R, Edelmann M, Kessler B, Yu V. Dia2 controls transcription by mediating assembly of the RSC complex. PLoS ONE. 2011;6:e21172 pubmed publisher
    b>Dia2 is an F-box protein found in the budding yeast, S. cerevisiae. Together with Skp1 and Cul1, Dia2 forms the substrate-determining part of an E3 ubiquitin ligase complex, otherwise known as the SCF...
  9. Mimura S, Yamaguchi T, Ishii S, Noro E, Katsura T, Obuse C, et al. Cul8/Rtt101 forms a variety of protein complexes that regulate DNA damage response and transcriptional silencing. J Biol Chem. 2010;285:9858-67 pubmed publisher
    ..These results suggest that Cul8-Mms1, as part of various protein complexes, is involved in the regulation of chromatin metabolism. ..

More Information


  1. Maric M, Maculins T, De Piccoli G, Labib K. Cdc48 and a ubiquitin ligase drive disassembly of the CMG helicase at the end of DNA replication. Science. 2014;346:1253596 pubmed publisher
    ..Here we show that the ubiquitin ligase known as SCF(Dia2) promotes ubiquitylation of CMG during the final stages of chromosome replication in Saccharomyces cerevisiae...
  2. Kim D, Zhang W, Koepp D. The Hect domain E3 ligase Tom1 and the F-box protein Dia2 control Cdc6 degradation in G1 phase. J Biol Chem. 2012;287:44212-20 pubmed publisher
    ..we identified two additional E3 ubiquitin ligase components required for Cdc6 degradation, the F-box protein Dia2 and the Hect domain E3 Tom1...
  3. Chaudhury I, Koepp D. Degradation of Mrc1 promotes recombination-mediated restart of stalled replication forks. Nucleic Acids Res. 2017;45:2558-2570 pubmed publisher
    ..Using DNA fiber analysis, we showed that Dia2 functions with the Sgs1 and Mph1 helicases (orthologs of human BLM and FANCM, respectively) in the recombination-..
  4. Gómez González B, Felipe Abrio I, Aguilera A. The S-phase checkpoint is required to respond to R-loops accumulated in THO mutants. Mol Cell Biol. 2009;29:5203-13 pubmed publisher
    ..In light of these results, we propose a model in which R-loop-mediated recombination is explained by template switching. ..
  5. Fong C, Arumugam A, Koepp D. The Saccharomyces cerevisiae F-box protein Dia2 is a mediator of S-phase checkpoint recovery from DNA damage. Genetics. 2013;193:483-99 pubmed publisher
    ..In this study, we identify the Saccharomyces cerevisiae F-box protein Dia2 as a novel player in the S-phase checkpoint recovery pathway...
  6. Kim D, Koepp D. Hect E3 ubiquitin ligase Tom1 controls Dia2 degradation during the cell cycle. Mol Biol Cell. 2012;23:4203-11 pubmed publisher
    The ubiquitin proteasome system plays a pivotal role in controlling the cell cycle. The budding yeast F-box protein Dia2 is required for genomic stability and is targeted for ubiquitin-dependent degradation in a cell cycle-dependent ..
  7. Maculins T, Nkosi P, Nishikawa H, Labib K. Tethering of SCF(Dia2) to the Replisome Promotes Efficient Ubiquitylation and Disassembly of the CMG Helicase. Curr Biol. 2015;25:2254-9 pubmed publisher
    ..In budding yeast, the F-box protein Dia2 drives ubiquitylation of the CMG helicase at the end of replication, leading to a disassembly pathway that requires ..
  8. Burgess R, Zhou H, Han J, Li Q, Zhang Z. The SCFDia2 ubiquitin E3 ligase ubiquitylates Sir4 and functions in transcriptional silencing. PLoS Genet. 2012;8:e1002846 pubmed publisher
    ..We show that cells lacking Dia2, a component of the SCF(Dia2) E3 ubiquitin ligase involved in DNA replication, display defects in silencing at the ..
  9. Bao M, Schwartz M, Cantin G, Yates J, Madhani H. Pheromone-dependent destruction of the Tec1 transcription factor is required for MAP kinase signaling specificity in yeast. Cell. 2004;119:991-1000 pubmed
    ..Destruction of Tec1 requires the F box protein Dia2 (Digs-into-agar-2), and Cdc53, the Cullin of SCF (Skp1-Cdc53-F box) ubiquitin ligases...