Gene Symbol: DEG1
Description: pseudouridine synthase DEG1
Alias: HRM3, PUS3, pseudouridine synthase DEG1
Species: Saccharomyces cerevisiae S288c
Copela L, Chakshusmathi G, Sherrer R, Wolin S. The La protein functions redundantly with tRNA modification enzymes to ensure tRNA structural stability. RNA. 2006;12:644-54 pubmed
..In addition, two pseudouridine synthases, PUS3 and PUS4, are important for growth in strains carrying a mutation in tRNA(Arg)(CCG) and are essential when La is ..
Han L, Kon Y, Phizicky E. Functional importance of Î¨38 and Î¨39 in distinct tRNAs, amplified for tRNAGln(UUG) by unexpected temperature sensitivity of the s2U modification in yeast. RNA. 2015;21:188-201 pubmed publisher
..in the anticodon stem-loop are frequently modified to pseudouridine (Î¨) by members of the widely conserved TruA/Pus3 family of pseudouridylases...
Lecointe F, Simos G, Sauer A, Hurt E, Motorin Y, Grosjean H. Characterization of yeast protein Deg1 as pseudouridine synthase (Pus3) catalyzing the formation of psi 38 and psi 39 in tRNA anticodon loop. J Biol Chem. 1998;273:1316-23 pubmed
The enzymatic activity of yeast gene product Deg1 was identified using both disrupted yeast strain and cloned recombinant protein expressed in yeast and in Escherichia coli...
Hepfer C, Arnold Croop S, Fogell H, Steudel K, Moon M, Roff A, et al
. DEG1, encoding the tRNA:pseudouridine synthase Pus3p, impacts HOT1-stimulated recombination in Saccharomyces cerevisiae. Mol Genet Genomics. 2005;274:528-38 pubmed
..The trans-acting nuclear mutation hrm3-1 specifically reduces HOT1-dependent recombination and prevents cell growth at 37 degrees ...
Sullivan J, Lewis M, Nikko E, Pelham H. Multiple interactions drive adaptor-mediated recruitment of the ubiquitin ligase rsp5 to membrane proteins in vivo and in vitro. Mol Biol Cell. 2007;18:2429-40 pubmed
..We suggest that sequential assembly of first a Bsd2/Rsp5 complex, then a Tre1/Bsd2/Rsp5 complex followed by a rearrangement of PY-WW interactions is required for the ubiquitination of Smf1. ..
Carlile T, Rojas Duran M, Zinshteyn B, Shin H, Bartoli K, Gilbert W. Pseudouridine profiling reveals regulated mRNA pseudouridylation in yeast and human cells. Nature. 2014;515:143-6 pubmed publisher
..Our findings reveal unanticipated roles for pseudouridylation and provide a resource for identifying the targets of pseudouridine synthases implicated in human disease. ..
Klassen R, Schaffrath R. Role of Pseudouridine Formation by Deg1 for Functionality of Two Glutamine Isoacceptor tRNAs. Biomolecules. 2017;7: pubmed publisher
Loss of Deg1/Pus3 and concomitant elimination of pseudouridine in tRNA at positions 38 and 39 (ψ38/39) was shown to specifically impair the function of tRNAGlnUUG under conditions of temperature-induced down-..