DBF2

Summary

Gene Symbol: DBF2
Description: serine/threonine-protein kinase DBF2
Alias: serine/threonine-protein kinase DBF2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Meitinger F, Palani S, Hub B, Pereira G. Dual function of the NDR-kinase Dbf2 in the regulation of the F-BAR protein Hof1 during cytokinesis. Mol Biol Cell. 2013;24:1290-304 pubmed publisher
    The conserved NDR-kinase Dbf2 plays a critical role in cytokinesis in budding yeast. Among its cytokinesis-related substrates is the F-BAR protein Hof1...
  2. Johnston L, Eberly S, Chapman J, Araki H, Sugino A. The product of the Saccharomyces cerevisiae cell cycle gene DBF2 has homology with protein kinases and is periodically expressed in the cell cycle. Mol Cell Biol. 1990;10:1358-66 pubmed
    ..In this paper, one of them, dbf2, is characterized in detail...
  3. Morishita T, Mitsuzawa H, Nakafuku M, Nakamura S, Hattori S, Anraku Y. Requirement of Saccharomyces cerevisiae Ras for completion of mitosis. Science. 1995;270:1213-5 pubmed
    ..The lethality of the triple disruption was suppressed by the multicopies of CDC5, CDC15, DBF2, SPO12, and TEM1, all of which function in the completion of the M phase...
  4. Shou W, Azzam R, Chen S, Huddleston M, Baskerville C, Charbonneau H, et al. Cdc5 influences phosphorylation of Net1 and disassembly of the RENT complex. BMC Mol Biol. 2002;3:3 pubmed
    ..However, net1(19m') cells exhibit a synergistic growth defect when combined with mutations in CDC5 or DBF2 (another MEN gene)...
  5. Donovan J, Toyn J, Johnson A, Johnston L. P40SDB25, a putative CDK inhibitor, has a role in the M/G1 transition in Saccharomyces cerevisiae. Genes Dev. 1994;8:1640-53 pubmed
    The Saccharomyces cerevisiae protein kinase Dbf2 carries out an essential function in late mitosis, and its kinase activity is cell-cycle regulated around anaphase/telophase...
  6. Visintin R, Craig K, Hwang E, Prinz S, Tyers M, Amon A. The phosphatase Cdc14 triggers mitotic exit by reversal of Cdk-dependent phosphorylation. Mol Cell. 1998;2:709-18 pubmed
    ..Feedback between these pathways may lead to precipitous collapse of mitotic CDK activity and help coordinate exit from mitosis. ..
  7. Mohl D, Huddleston M, Collingwood T, Annan R, Deshaies R. Dbf2-Mob1 drives relocalization of protein phosphatase Cdc14 to the cytoplasm during exit from mitosis. J Cell Biol. 2009;184:527-39 pubmed publisher
    ..In this study, we show that a MEN component, protein kinase Dbf2-Mob1, promotes transfer of Cdc14 to the cytoplasm and consequent exit from mitosis by direct phosphorylation of ..
  8. Mah A, Jang J, Deshaies R. Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex. Proc Natl Acad Sci U S A. 2001;98:7325-30 pubmed
    ..turn, is regulated by a group of proteins, the mitotic exit network (MEN), which includes Lte1, Tem1, Cdc5, Cdc15, Dbf2/Dbf20, and Mob1. The direct biochemical interactions between the components of the MEN remain largely unresolved...
  9. Jaspersen S, Morgan D. Cdc14 activates cdc15 to promote mitotic exit in budding yeast. Curr Biol. 2000;10:615-8 pubmed
    ..Instead, Cdc15 dephosphorylation may promote some additional function of Cdc15 that is independent of its effects on Cdc14 activation. ..

More Information

Publications53

  1. Frenz L, Lee S, Fesquet D, Johnston L. The budding yeast Dbf2 protein kinase localises to the centrosome and moves to the bud neck in late mitosis. J Cell Sci. 2000;113 Pt 19:3399-408 pubmed
    b>Dbf2 is a multifunctional protein kinase in Saccharomyces cerevisiae that functions in transcription, the stress response and as part of a network of genes in exit from mitosis...
  2. Meitinger F, Boehm M, Hofmann A, Hub B, Zentgraf H, Lehmann W, et al. Phosphorylation-dependent regulation of the F-BAR protein Hof1 during cytokinesis. Genes Dev. 2011;25:875-88 pubmed publisher
    ..Here we identified the conserved F-BAR protein Hof1 as a substrate of the MEN kinase complex Dbf2-Mob1 during cytokinesis...
  3. Toyn J, Johnston L. The Dbf2 and Dbf20 protein kinases of budding yeast are activated after the metaphase to anaphase cell cycle transition. EMBO J. 1994;13:1103-13 pubmed
    Thermosensitive mutations in the DBF2 gene arrest the cell cycle during nuclear division...
  4. Toyn J, Johnson A, Donovan J, Toone W, Johnston L. The Swi5 transcription factor of Saccharomyces cerevisiae has a role in exit from mitosis through induction of the cdk-inhibitor Sic1 in telophase. Genetics. 1997;145:85-96 pubmed
    ..Second, both are hypersensitive to overexpression of the B cyclin CLB2. Thus Swi5-dependent activation of the SIC1 gene contributes to the deactivation of the B cyclin kinase, and hence exit from mitosis. ..
  5. Meitinger F, Petrova B, Lombardi I, Bertazzi D, Hub B, Zentgraf H, et al. Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic-exit network regulates cytokinesis in budding yeast. J Cell Sci. 2010;123:1851-61 pubmed publisher
    ..This defect was partially suppressed by increased levels of Cyk3. We therefore propose that the MEN directly controls cytokinesis via targeting of Inn1, Cyk3 and Chs2 to the bud neck...
  6. Jaspersen S, Charles J, Tinker Kulberg R, Morgan D. A late mitotic regulatory network controlling cyclin destruction in Saccharomyces cerevisiae. Mol Biol Cell. 1998;9:2803-17 pubmed
    ..yeast Saccharomyces cerevisiae, members of a large class of late mitotic mutants, including cdc15, cdc5, cdc14, dbf2, and tem1, arrest in anaphase with a phenotype similar to that of cells expressing nondegradable forms of mitotic ..
  7. Hwa Lim H, Yeong F, Surana U. Inactivation of mitotic kinase triggers translocation of MEN components to mother-daughter neck in yeast. Mol Biol Cell. 2003;14:4734-43 pubmed
    ..is regulated by the mitotic exit network (MEN), which includes a GTPase (Tem1) and various kinases (Cdc15, Cdc5, Dbf2, and Dbf20). Here, we show that Dbf2 and Dbf20 functions are necessary for the execution of cytokinesis...
  8. Wang Y, Shirogane T, Liu D, Harper J, Elledge S. Exit from exit: resetting the cell cycle through Amn1 inhibition of G protein signaling. Cell. 2003;112:697-709 pubmed
    ..Thus, Amn1 is part of a daughter-specific switch that helps cells exit from mitotic exit and reset the cell cycle. ..
  9. Shou W, Deshaies R. Multiple telophase arrest bypassed (tab) mutants alleviate the essential requirement for Cdc15 in exit from mitosis in S. cerevisiae. BMC Genet. 2002;3:4 pubmed
    ..Transport-defective mutations in two karyopherins can bypass cdc15Delta, suggesting that the function of the MEN is to promote mitotic exit by regulating nuclear transport. ..
  10. Stoepel J, Ottey M, Kurischko C, Hieter P, Luca F. The mitotic exit network Mob1p-Dbf2p kinase complex localizes to the nucleus and regulates passenger protein localization. Mol Biol Cell. 2005;16:5465-79 pubmed
    ..These findings reveal kinetochores as sites for MEN signaling and implicate MEN in coordinating chromosome segregation and/or spindle integrity with mitotic exit and cytokinesis via regulation of chromosome passenger proteins. ..
  11. Yoshida S, Toh e A. Regulation of the localization of Dbf2 and mob1 during cell division of saccharomyces cerevisiae. Genes Genet Syst. 2001;76:141-7 pubmed
    ..yeast, MEN consists of the protein phosphatase Cdc14, the ras-like GTPase Tem1, protein kinases Cdc15, Cdc5, Dbf2 and Dbf2-binding protein Mob1...
  12. Asakawa K, Toh e A. A defect of Kap104 alleviates the requirement of mitotic exit network gene functions in Saccharomyces cerevisiae. Genetics. 2002;162:1545-56 pubmed
    ..These results suggest that a mutation in Kap104 stimulates exit from mitosis through the activation of Cdc14 and implies a novel role for Kap104 in cell-cycle progression in budding yeast. ..
  13. Corbett M, Xiong Y, Boyne J, Wright D, Munro E, Price C. IQGAP and mitotic exit network (MEN) proteins are required for cytokinesis and re-polarization of the actin cytoskeleton in the budding yeast, Saccharomyces cerevisiae. Eur J Cell Biol. 2006;85:1201-15 pubmed
    ..These data establish a mechanistic dependency of cell separation upon an intermediate step requiring actomyosin ring assembly. ..
  14. Liu H, Toyn J, Chiang Y, Draper M, Johnston L, Denis C. DBF2, a cell cycle-regulated protein kinase, is physically and functionally associated with the CCR4 transcriptional regulatory complex. EMBO J. 1997;16:5289-98 pubmed
    ..Using the yeast two-hybrid screen, we have identified DBF2, a cell cycle-regulated protein kinase, as a CCR4-associated protein...
  15. Oh Y, Chang K, Orlean P, Wloka C, Deshaies R, Bi E. Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast. Mol Biol Cell. 2012;23:2445-56 pubmed publisher
    ..Here, we report that Dbf2, a conserved mitotic exit kinase, localizes to the division site after Chs2 and directly phosphorylates Chs2 on ..
  16. Shirayama M, Matsui Y, Toh e A. The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase. Mol Cell Biol. 1994;14:7476-82 pubmed
    ..The genetic interaction among LTE1, TEM1, and CDC15 indicates that they cooperatively play an essential role for termination of M phase. ..
  17. Rahal R, Amon A. The Polo-like kinase Cdc5 interacts with FEAR network components and Cdc14. Cell Cycle. 2008;7:3262-72 pubmed
    ..Finally, we present evidence that the Cdc5-Cdc14 association is direct, further supporting the central role of Cdc5 in Cdc14 localization. ..
  18. Visintin R, Hwang E, Amon A. Cfi1 prevents premature exit from mitosis by anchoring Cdc14 phosphatase in the nucleolus. Nature. 1999;398:818-23 pubmed
    ..A highly conserved signalling cascade, critical for the exit from mitosis, is required for this movement of Cdc14 during anaphase. Furthermore, we have identified a negative regulator of Cdc14, Cfi1, that anchors Cdc14 in the nucleolus. ..
  19. Visintin R, Amon A. Regulation of the mitotic exit protein kinases Cdc15 and Dbf2. Mol Biol Cell. 2001;12:2961-74 pubmed
    ..factor Lte1, the GTPase activating protein complex Bub2-Bfa1/Byr4, and several protein kinases including Cdc15 and Dbf2. Here we investigate the regulation of the protein kinases Dbf2 and Cdc15...
  20. Chen J, Rappsilber J, Chiang Y, Russell P, Mann M, Denis C. Purification and characterization of the 1.0 MDa CCR4-NOT complex identifies two novel components of the complex. J Mol Biol. 2001;314:683-94 pubmed
    ..The distinct location of CAF40 and CAF130 and the evolutionary conservation of CAF40 implicate them in novel roles in the function of the CCR4-NOT complex. ..
  21. Komarnitsky S, Chiang Y, Luca F, Chen J, Toyn J, Winey M, et al. DBF2 protein kinase binds to and acts through the cell cycle-regulated MOB1 protein. Mol Cell Biol. 1998;18:2100-7 pubmed
    The DBF2 gene of the budding yeast Saccharomyces cerevisiae encodes a cell cycle-regulated protein kinase that plays an important role in the telophase/G1 transition...
  22. Shah R, Jensen S, Frenz L, Johnson A, Johnston L. The Spo12 protein of Saccharomyces cerevisiae: a regulator of mitotic exit whose cell cycle-dependent degradation is mediated by the anaphase-promoting complex. Genetics. 2001;159:965-80 pubmed
    ..This result is the first indication that Spo12 may carry out the same biochemical function in mitosis as it does in meiosis. ..
  23. Geymonat M, Spanos A, de Bettignies G, Sedgwick S. Lte1 contributes to Bfa1 localization rather than stimulating nucleotide exchange by Tem1. J Cell Biol. 2009;187:497-511 pubmed publisher
    ..We observed that lte1 mutants display aberrant localization of the polarity cap, which is the organizer of the actin cytoskeleton. We propose that Lte1's role in cell polarization underlies its contribution to mitotic regulation. ..
  24. Parkes V, Johnston L. SPO12 and SIT4 suppress mutations in DBF2, which encodes a cell cycle protein kinase that is periodically expressed. Nucleic Acids Res. 1992;20:5617-23 pubmed
    To help clarify the role of DBF2, a previously described cell cycle protein kinase, high copy number suppressors of the dbf2 mutation were isolated. Three open reading frames (ORF) have been identified...
  25. Toyn J, Araki H, Sugino A, Johnston L. The cell-cycle-regulated budding yeast gene DBF2, encoding a putative protein kinase, has a homologue that is not under cell-cycle control. Gene. 1991;104:63-70 pubmed
    The budding yeast cell-cycle gene, DBF2, encoding a putative protein kinase, was shown to have a homologue, designated DBF20...
  26. König C, Maekawa H, Schiebel E. Mutual regulation of cyclin-dependent kinase and the mitotic exit network. J Cell Biol. 2010;188:351-68 pubmed publisher
    ..In addition, Cdk1 phosphorylates the Mob1 protein to inhibit the activity of Dbf2-Mob1 kinase that regulates Cdc14 phosphatase...
  27. Rossio V, Galati E, Ferrari M, Pellicioli A, Sutani T, Shirahige K, et al. The RSC chromatin-remodeling complex influences mitotic exit and adaptation to the spindle assembly checkpoint by controlling the Cdc14 phosphatase. J Cell Biol. 2010;191:981-97 pubmed publisher
    ..Our data suggest that fine-tuning regulators of mitotic exit have important functions during mitotic progression in cells treated with microtubule poisons and might be promising targets for cancer treatment. ..
  28. Irniger S, Baumer M, Braus G. Glucose and ras activity influence the ubiquitin ligases APC/C and SCF in Saccharomyces cerevisiae. Genetics. 2000;154:1509-21 pubmed
    ..We conclude that glucose and Ras proteins influence the APC/C and the SCF complex in an opposite manner. These ubiquitin ligases might represent novel targets for modulating cell division in response to growth conditions. ..
  29. Hotz M, Leisner C, Chen D, Manatschal C, Wegleiter T, Ouellet J, et al. Spindle pole bodies exploit the mitotic exit network in metaphase to drive their age-dependent segregation. Cell. 2012;148:958-72 pubmed publisher
    ..Consistent with this, phosphorylation of Kar9 by the MEN kinases Dbf2 and Dbf20 is not required to break Kar9 symmetry but is instead required to maintain stable association of Kar9 ..
  30. Renicke C, Allmann A, Lutz A, Heimerl T, Taxis C. The Mitotic Exit Network Regulates Spindle Pole Body Selection During Sporulation of Saccharomyces cerevisiae. Genetics. 2017;206:919-937 pubmed publisher
    ..Moreover, efficient genome inheritance requires Dbf2/20-Mob1 during a late step in spore maturation...
  31. Toyn J, Johnston L. Spo12 is a limiting factor that interacts with the cell cycle protein kinases Dbf2 and Dbf20, which are involved in mitotic chromatid disjunction. Genetics. 1993;135:963-71 pubmed
    The DBF2 and DBF20 genes of the budding yeast Saccharomyces cerevisiae encode a pair of structurally similar protein kinases. Although yeast with either gene deleted is viable, deletion of both genes is lethal...
  32. Kitada K, Johnson A, Johnston L, Sugino A. A multicopy suppressor gene of the Saccharomyces cerevisiae G1 cell cycle mutant gene dbf4 encodes a protein kinase and is identified as CDC5. Mol Cell Biol. 1993;13:4445-57 pubmed
    ..CDC5 on a multicopy plasmid also suppresses temperature-sensitive cdc15, cdc20, and dbf2 mutations which affect mitosis during the cell cycle.
  33. Grandin N, de Almeida A, Charbonneau M. The Cdc14 phosphatase is functionally associated with the Dbf2 protein kinase in Saccharomyces cerevisiae. Mol Gen Genet. 1998;258:104-16 pubmed
    The Saccharomyces cerevisiae Cdc14 protein phosphatase and Dbf2 protein kinase have been implicated to act during late M phase, but their functions are not known...
  34. Fitzpatrick P, Toyn J, Millar J, Johnston L. DNA replication is completed in Saccharomyces cerevisiae cells that lack functional Cdc14, a dual-specificity protein phosphatase. Mol Gen Genet. 1998;258:437-41 pubmed
    ..Overexpression of Sicl, a cyclin-dependent protein kinase inhibitor, is able to suppress telophase mutants such as dbf2, cdc5 and cdc15, but not cdc14...
  35. Chen Y, Weinreich M. Dbf4 regulates the Cdc5 Polo-like kinase through a distinct non-canonical binding interaction. J Biol Chem. 2010;285:41244-54 pubmed publisher
    ..This study, therefore, details the molecular nature of a new type of PBD binding and reveals that Cdc5 targeting to phosphorylated substrates likely regulates spindle dynamics. ..
  36. Makrantoni V, Dennison P, Stark M, Coote P. A novel role for the yeast protein kinase Dbf2p in vacuolar H+-ATPase function and sorbic acid stress tolerance. Microbiology. 2007;153:4016-26 pubmed
    ..acid hypersensitivity and impaired vacuolar acidification, phenotypes also observed in both a kinase-inactive dbf2 mutant and cells completely lacking DBF2 (dbf2Delta)...
  37. Grandin N, Charbonneau M. Dbf2 is implicated in a Cbt1-dependent pathway following a shift from glucose to galactose or non-fermentable carbon sources in Saccharomyces cerevisiae. Mol Gen Genet. 1999;261:402-7 pubmed
    b>Dbf2, a cell cycle-regulated protein kinase, has been shown recently to be part of the CCR4 transcriptional regulatory complex in the yeast Saccharomyces cerevisiae...
  38. Yellman C, Roeder G. Cdc14 Early Anaphase Release, FEAR, Is Limited to the Nucleus and Dispensable for Efficient Mitotic Exit. PLoS ONE. 2015;10:e0128604 pubmed publisher
    ..Our findings clarify the distinction between FEAR and MEN-dependent Cdc14 activities and will help guide emerging quantitative models of this cell cycle transition. ..
  39. Drechsler H, Tan A, Liakopoulos D. Yeast GSK-3 kinase regulates astral microtubule function through phosphorylation of the microtubule-stabilizing kinesin Kip2. J Cell Sci. 2015;128:3910-21 pubmed publisher
    ..We propose that yeast GSK-3 spatially controls astral MT dynamics and the loading of dynein and Kar9 on astral MT plus ends by regulating Kip2 interactions with Bim1 and MTs. ..
  40. Liu H, Badarinarayana V, Audino D, Rappsilber J, Mann M, Denis C. The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively. EMBO J. 1998;17:1096-106 pubmed
    The CCR4 transcriptional regulatory complex consisting of CCR4, CAF1, DBF2 and other unidentified factors is one of several groups of proteins that affect gene expression...
  41. Attner M, Amon A. Control of the mitotic exit network during meiosis. Mol Biol Cell. 2012;23:3122-32 pubmed publisher
  42. Wolken D, McInnes J, Pon L. Aim44p regulates phosphorylation of Hof1p to promote contractile ring closure during cytokinesis in budding yeast. Mol Biol Cell. 2014;25:753-62 pubmed publisher
    ..Our studies reveal a novel role for Aim44p in regulating contractile ring closure through effects on Hof1p. ..
  43. Rodriguez Rodriguez J, Moyano Y, Játiva S, Queralt E. Mitotic Exit Function of Polo-like Kinase Cdc5 Is Dependent on Sequential Activation by Cdk1. Cell Rep. 2016;15:2050-62 pubmed publisher
    ..Cdk1 first phosphorylates Cdc5 to activate it in early anaphase, and then, in late anaphase, further phosphorylation of Cdc5 by Cdk1 is needed to promote its MEN-related functions. ..
  44. Liu H, Chiang Y, Pan J, Chen J, Salvadore C, Audino D, et al. Characterization of CAF4 and CAF16 reveals a functional connection between the CCR4-NOT complex and a subset of SRB proteins of the RNA polymerase II holoenzyme. J Biol Chem. 2001;276:7541-8 pubmed
    ..9-MDa complex. CAF4 also displayed physical interactions with multiple CCR4-NOT components and with DBF2, a likely component of the 1.9-MDa complex...