Gene Symbol: CYT1
Description: ubiquinol--cytochrome-c reductase catalytic subunit CYT1
Alias: CTC1, YOR29-16, ubiquinol--cytochrome-c reductase catalytic subunit CYT1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Corvest V, Murrey D, Bernard D, Knaff D, Guiard B, Hamel P. c-type cytochrome assembly in Saccharomyces cerevisiae: a key residue for apocytochrome c1/lyase interaction. Genetics. 2010;186:561-71 pubmed publisher
    ..CC(1)HL-dependent assembly of holocytochrome c(1). We also provide evidence that Cyc2p is dedicated to the CCHL pathway and is not required for the CC(1)HL-dependent assembly of cytochrome c(1). ..
  2. Bernard D, Quevillon Cheruel S, Merchant S, Guiard B, Hamel P. Cyc2p, a membrane-bound flavoprotein involved in the maturation of mitochondrial c-type cytochromes. J Biol Chem. 2005;280:39852-9 pubmed
    ..We postulate that, in vivo, Cyc2p interacts with CCHL and is involved in the reduction of heme prior to its ligation to apocytochrome c by CCHL. ..
  3. Hunte C, Koepke J, Lange C, Rossmanith T, Michel H. Structure at 2.3 A resolution of the cytochrome bc(1) complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment. Structure. 2000;8:669-84 pubmed
    ..Each 'monomer' of the refined model includes 2178 amino acid residues of subunits COR1, QCR2, COB, CYT1, RIP1, QCR6, QCR7, QCR8 and QCR9 of the cytochrome bc(1) complex and of the polypeptides V(H) and V(L) of the Fv ..
  4. Mick D, Wagner K, van der Laan M, Frazier A, Perschil I, Pawlas M, et al. Shy1 couples Cox1 translational regulation to cytochrome c oxidase assembly. EMBO J. 2007;26:4347-58 pubmed
    ..We suggest that Shy1 links Cox1 translational regulation to complex IV assembly and supercomplex formation. ..
  5. Saint Georges Y, Hamel P, Lemaire C, Dujardin G. Role of positively charged transmembrane segments in the insertion and assembly of mitochondrial inner-membrane proteins. Proc Natl Acad Sci U S A. 2001;98:13814-9 pubmed
    ..This model argues in favor of a role of Oxa1p in the insertion and the lateral exit of less hydrophobic TM domains from the translocation site into the lipid bilayer. ..
  6. Mick D, Vukotic M, Piechura H, Meyer H, Warscheid B, Deckers M, et al. Coa3 and Cox14 are essential for negative feedback regulation of COX1 translation in mitochondria. J Cell Biol. 2010;191:141-54 pubmed publisher
    ..Our data indicate that Coa1 binding to sequestered Mss51 in complex with Cox14, Coa3, and Cox1 is essential for full inactivation. ..
  7. Wang X, Zuo X, Kucejova B, Chen X. Reduced cytosolic protein synthesis suppresses mitochondrial degeneration. Nat Cell Biol. 2008;10:1090-7 pubmed
    ..Our finding thus establishes a link between protein homeostasis (proteostasis), cellular bioenergetics and mitochondrial maintenance during ageing. ..
  8. Chen Y, Taylor E, Dephoure N, Heo J, Tonhato A, Papandreou I, et al. Identification of a protein mediating respiratory supercomplex stability. Cell Metab. 2012;15:348-60 pubmed publisher
    ..We suggest that Rcf1 is a member of an evolutionarily conserved protein family that acts to promote respiratory supercomplex assembly and activity. ..
  9. Strogolova V, Furness A, Robb McGrath M, Garlich J, Stuart R. Rcf1 and Rcf2, members of the hypoxia-induced gene 1 protein family, are critical components of the mitochondrial cytochrome bc1-cytochrome c oxidase supercomplex. Mol Cell Biol. 2012;32:1363-73 pubmed publisher
    ..Based on our findings, we propose a model where the Hig1 proteins regulate the COX enzyme activity through Cox3 and associated Cox12 protein, in a manner that may be influenced by the neighboring AAC proteins. ..

More Information


  1. Conte A, Papa B, Ferramosca A, Zara V. The dimerization of the yeast cytochrome bc1 complex is an early event and is independent of Rip1. Biochim Biophys Acta. 2015;1853:987-95 pubmed publisher
    ..Finally, a new model of cytochrome bc1 complex assembly, in which distinct intermediates sequentially interact during bc1 maturation, has been proposed. ..
  2. Hamel P, Lemaire C, Bonnefoy N, Brivet Chevillotte P, Dujardin G. Mutations in the membrane anchor of yeast cytochrome c1 compensate for the absence of Oxa1p and generate carbonate-extractable forms of cytochrome c1. Genetics. 1998;150:601-11 pubmed
    ..Molecular cloning and sequencing reveal that the suppressor mutations (CYT1-1 to -6) correspond to amino acid substitutions that are all located in the membrane anchor of cytochrome c1 and ..
  3. Galdieri L, Zhang T, Rogerson D, Vancura A. Reduced Histone Expression or a Defect in Chromatin Assembly Induces Respiration. Mol Cell Biol. 2016;36:1064-77 pubmed publisher
    ..Together, our data indicate that altered chromatin structure relieves glucose repression of mitochondrial respiration by inducing transcription of the TCA cycle and OXPHOS genes carried by both nuclear and mitochondrial DNA. ..
  4. Hunte C, Solmaz S, Lange C. Electron transfer between yeast cytochrome bc(1) complex and cytochrome c: a structural analysis. Biochim Biophys Acta. 2002;1555:21-8 pubmed
    ..Remarkably, CYC binds only to one of the two possible binding sites of the homodimeric complex and binding appears to be coordinated with the presence of ubiquinone at the Q(i) site. Regulatory aspects of CYC reduction are discussed. ..
  5. Morar A, Wang X, Pielak G. Effects of crowding by mono-, di-, and tetrasaccharides on cytochrome c-cytochrome c peroxidase binding: comparing experiment to theory. Biochemistry. 2001;40:281-5 pubmed
    ..None of the analyses are completely successful by themselves, and the results suggest that a complete analysis must account for both excluded-volume and chemical interactions. ..
  6. Ahmad Z, Sherman F. Role of Arg-166 in yeast cytochrome C1. J Biol Chem. 2001;276:18450-6 pubmed
    A systematic screen for dominant-negative mutations of the CYT1 gene, which encodes cytochrome c(1), revealed seven mutants after testing approximately 10(4) Saccharomyces cerevisiae strains transformed with a library of mutagenized ..
  7. Atkinson A, Smith P, Fox J, Cui T, Khalimonchuk O, Winge D. The LYR protein Mzm1 functions in the insertion of the Rieske Fe/S protein in yeast mitochondria. Mol Cell Biol. 2011;31:3988-96 pubmed publisher
  8. Waldmeyer B, Bechtold R, Bosshard H, Poulos T. The cytochrome c peroxidase.cytochrome c electron transfer complex. Experimental support of a hypothetical model. J Biol Chem. 1982;257:6073-6 pubmed
    ..Cross-linking occurred to carboxyl groups of the NH2-terminal fragment 1-119 and of fragment 172-229. The four crucial aspartates of the hypothetical model are located in these same two sequence regions. ..
  9. Römpler K, Müller T, Juris L, Wissel M, Vukotic M, Hofmann K, et al. Overlapping Role of Respiratory Supercomplex Factor Rcf2 and Its N-terminal Homolog Rcf3 in Saccharomyces cerevisiae. J Biol Chem. 2016;291:23769-23778 pubmed
    ..Furthermore, our data suggest an association of all three Rcf proteins with the bc1 complex in the absence of a functional cytochrome c oxidase and identify a supercomplex independent interaction network of the Rcf proteins. ..
  10. Hildenbeutel M, Hegg E, Stephan K, Gruschke S, Meunier B, Ott M. Assembly factors monitor sequential hemylation of cytochrome b to regulate mitochondrial translation. J Cell Biol. 2014;205:511-24 pubmed publisher
    ..Failure to hemylate cytochrome b sequesters the Cbp3-Cbp6 complex in early assembly intermediates, thereby causing a reduction in cytochrome b synthesis via a feedback loop that senses hemylation of cytochrome b. ..
  11. Lavoie H, Whiteway M. Increased respiration in the sch9Delta mutant is required for increasing chronological life span but not replicative life span. Eukaryot Cell. 2008;7:1127-35 pubmed publisher
    ..Deletion of HAP4 and CYT1, both of which are essential for respiration, revert the sch9Delta mutant respiratory rate back to a lower-than-..
  12. Oyedotun K, Yau P, Lemire B. Identification of the heme axial ligands in the cytochrome b562 of the Saccharomyces cerevisiae succinate dehydrogenase. J Biol Chem. 2004;279:9432-9 pubmed
    ..Loss of cytochrome b(562) has no effect on enzyme assembly and quinone reduction; the role of the heme in enzyme structure and function is discussed. ..
  13. Easlon E, Tsang F, Skinner C, Wang C, Lin S. The malate-aspartate NADH shuttle components are novel metabolic longevity regulators required for calorie restriction-mediated life span extension in yeast. Genes Dev. 2008;22:931-44 pubmed publisher
    ..Together, these data suggest that CR may extend life span and ameliorate age-associated metabolic diseases by activating components of the NADH shuttles. ..
  14. Lauffer S, Mäbert K, Czupalla C, Pursche T, Hoflack B, Rödel G, et al. Saccharomyces cerevisiae porin pore forms complexes with mitochondrial outer membrane proteins Om14p and Om45p. J Biol Chem. 2012;287:17447-58 pubmed publisher
    ..We propose a model for the role of the two OM proteins in addressing the porin pore to bind to specific channels in the mt IM to facilitate transport of metabolites. ..