CYK3

Summary

Gene Symbol: CYK3
Description: Cyk3p
Alias: Cyk3p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Ko N, Nishihama R, Tully G, Ostapenko D, Solomon M, Morgan D, et al. Identification of yeast IQGAP (Iqg1p) as an anaphase-promoting-complex substrate and its role in actomyosin-ring-independent cytokinesis. Mol Biol Cell. 2007;18:5139-53 pubmed
    ..Together, the data suggest that compromise of APC/C function allows the accumulation of Iqg1p, which then promotes actomyosin-ring-independent cytokinesis at least in part by activation of Cyk3p.
  2. Labedzka K, Tian C, Nussbaumer U, Timmermann S, Walther P, Müller J, et al. Sho1p connects the plasma membrane with proteins of the cytokinesis network through multiple isomeric interaction states. J Cell Sci. 2012;125:4103-13 pubmed publisher
    ..The proteins Hof1p, Cyk3p, Inn1p and Myo1p each represent one of the four genetically defined and partially complementary pathways of ..
  3. Korinek W, Bi E, Epp J, Wang L, Ho J, Chant J. Cyk3, a novel SH3-domain protein, affects cytokinesis in yeast. Curr Biol. 2000;10:947-50 pubmed
    ..To uncover this mechanism, a screen for high-copy suppressors of the iqg1 lethal phenotype was performed. CYK3 suppressed the requirement for IQG1 in viability and cytokinesis without restoration of the actin ring, ..
  4. Nishihama R, Schreiter J, Onishi M, Vallen E, Hanna J, Moravcevic K, et al. Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae. J Cell Biol. 2009;185:995-1012 pubmed publisher
    ..functionally important interactions with SH3 domains in the cytokinesis proteins Hof1 (an F-BAR protein) and Cyk3 (whose overexpression can restore PS formation in inn1Delta cells)...
  5. Jendretzki A, Ciklic I, Rodicio R, Schmitz H, Heinisch J. Cyk3 acts in actomyosin ring independent cytokinesis by recruiting Inn1 to the yeast bud neck. Mol Genet Genomics. 2009;282:437-51 pubmed publisher
    ..In addition, an actomyosin ring independent cytokinesis pathway exists in yeast. We here identified Cyk3, a key component of the alternative pathway, as a novel interaction partner of Inn1...
  6. Meitinger F, Petrova B, Lombardi I, Bertazzi D, Hub B, Zentgraf H, et al. Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic-exit network regulates cytokinesis in budding yeast. J Cell Sci. 2010;123:1851-61 pubmed publisher
    ..Here, we show that the MEN controls components involved in septum formation, including Inn1, Cyk3 and Chs2...
  7. Meitinger F, Boehm M, Hofmann A, Hub B, Zentgraf H, Lehmann W, et al. Phosphorylation-dependent regulation of the F-BAR protein Hof1 during cytokinesis. Genes Dev. 2011;25:875-88 pubmed publisher
    ..Genetic and functional data support a model in which Dbf2-Mob1 regulates Hof1 by inducing domain rearrangements, leading to the exposure of the Hof1 RLS domain during telophase. ..
  8. Palani S, Meitinger F, Boehm M, Lehmann W, Pereira G. Cdc14-dependent dephosphorylation of Inn1 contributes to Inn1-Cyk3 complex formation. J Cell Sci. 2012;125:3091-6 pubmed publisher
    ..the C-terminal proline-rich domain of Inn1 that mediates its binding to the SH3-domain-containing proteins Hof1 and Cyk3. We show that phosphorylation of Inn1 by Cdk1 partially perturbs the interaction of Inn1 with Cyk3 thereby reducing ..
  9. Oh Y, Chang K, Orlean P, Wloka C, Deshaies R, Bi E. Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast. Mol Biol Cell. 2012;23:2445-56 pubmed publisher
    ..Of interest, both chs2-S217A and chs2-S217D mutants are robustly suppressed by increased dosage of Cyk3, a cytokinesis protein that displays Dbf2-dependent localization and also stimulates Chs2-mediated chitin synthesis...

More Information

Publications21

  1. Meitinger F, Palani S, Hub B, Pereira G. Dual function of the NDR-kinase Dbf2 in the regulation of the F-BAR protein Hof1 during cytokinesis. Mol Biol Cell. 2013;24:1290-304 pubmed publisher
    ..Thus our data show that Dbf2 coordinates septin and AMR functions during cytokinesis through the regulation/control of Hof1. ..
  2. Foltman M, Molist I, Arcones I, Sacristan C, Filali Mouncef Y, Roncero C, et al. Ingression Progression Complexes Control Extracellular Matrix Remodelling during Cytokinesis in Budding Yeast. PLoS Genet. 2016;12:e1005864 pubmed publisher
    ..the IQGAP protein Iqg1 and Chs2, IPCs contain the F-BAR protein Hof1, and the cytokinesis regulators Inn1 and Cyk3. We describe the molecular mechanism by which chitin synthase is activated by direct association of the C2 domain ..
  3. Roelants F, Su B, von Wulffen J, Ramachandran S, Sartorel E, TROTT A, et al. Protein kinase Gin4 negatively regulates flippase function and controls plasma membrane asymmetry. J Cell Biol. 2015;208:299-311 pubmed publisher
  4. Tian C, Wu Y, Johnsson N. Stepwise and cooperative assembly of a cytokinetic core complex in Saccharomyces cerevisiae. J Cell Sci. 2014;127:3614-24 pubmed publisher
    ..We propose that the MIH complex acts as hinge between the AMR and the proteins involved in cell wall synthesis and membrane attachment. ..
  5. Cassani C, Raspelli E, Santo N, Chiroli E, Lucchini G, Fraschini R. Saccharomyces cerevisiae Dma proteins participate in cytokinesis by controlling two different pathways. Cell Cycle. 2013;12:2794-808 pubmed publisher
    ..be required for AMR contraction, and causes asymmetric primary septum deposition as well as mislocalization of the Cyk3-positive regulator of this process...
  6. Mancini Lombardi I, Palani S, Meitinger F, Darieva Z, Hofmann A, Sharrocks A, et al. Lre1 directly inhibits the NDR/Lats kinase Cbk1 at the cell division site in a phosphorylation-dependent manner. Curr Biol. 2013;23:1736-45 pubmed publisher
    ..We propose that similar inhibitory proteins may also provide fine tuning for the activity of NDR kinases in other organisms. ..
  7. Onishi M, Ko N, Nishihama R, Pringle J. Distinct roles of Rho1, Cdc42, and Cyk3 in septum formation and abscission during yeast cytokinesis. J Cell Biol. 2013;202:311-29 pubmed publisher
    ..Moreover, Rho1 is temporarily inactivated during cleavage-furrow ingression; this inactivation requires the protein Cyk3, which binds Rho1-guanosine diphosphate via its catalytically inactive transglutaminase-like domain...
  8. Nkosi P, Targosz B, Labib K, Sanchez Diaz A. Hof1 and Rvs167 have redundant roles in actomyosin ring function during cytokinesis in budding yeast. PLoS ONE. 2013;8:e57846 pubmed publisher
    ..The redundant role of Hof1 in actin ring assembly suggests that the mechanism of actin ring assembly has been conserved to a greater extent across evolution than anticipated previously. ..
  9. Atkins B, Yoshida S, Saito K, Wu C, Lew D, Pellman D. Inhibition of Cdc42 during mitotic exit is required for cytokinesis. J Cell Biol. 2013;202:231-40 pubmed publisher
    ..The effects of Cdc42 hyperactivation are largely mediated by the Cdc42 effector p21-activated kinase Ste20. Inhibition of Cdc42 and related Rho guanosine triphosphatases may be a general feature of cytokinesis in eukaryotes. ..
  10. Oh Y, Schreiter J, Nishihama R, Wloka C, Bi E. Targeting and functional mechanisms of the cytokinesis-related F-BAR protein Hof1 during the cell cycle. Mol Biol Cell. 2013;24:1305-20 pubmed publisher
  11. Devrekanli A, Foltman M, Roncero C, Sanchez Diaz A, Labib K. Inn1 and Cyk3 regulate chitin synthase during cytokinesis in budding yeasts. J Cell Sci. 2012;125:5453-66 pubmed publisher
    ..mutations in the Inn1 C2-domain, the dominant CHS2 alleles suppress cytokinesis defects produced by the lack of the Cyk3 protein...
  12. Amaral N, Vendrell A, Funaya C, Idrissi F, Maier M, Kumar A, et al. The Aurora-B-dependent NoCut checkpoint prevents damage of anaphase bridges after DNA replication stress. Nat Cell Biol. 2016;18:516-26 pubmed publisher
    ..Therefore, the molecular origin of chromatin bridges is critical for activation of NoCut, which plays a key role in the maintenance of genome stability after replicative stress. ..