Gene Symbol: CYB2
Description: Cyb2p
Alias: FCB2, Cyb2p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Guiard B. Structure, expression and regulation of a nuclear gene encoding a mitochondrial protein: the yeast L(+)-lactate cytochrome c oxidoreductase (cytochrome b2). EMBO J. 1985;4:3265-72 pubmed
    ..This is the first reported mutation affecting the structural gene of cytochrome b2. ..
  2. Daum G, Böhni P, Schatz G. Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria. J Biol Chem. 1982;257:13028-33 pubmed
    ..The availability of submitochondrial fractions provides a basis for studying import of precursor polypeptides into isolated yeast mitochondria. ..
  3. Tsai C, Gokulan K, Sobrado P, Sacchettini J, Fitzpatrick P. Mechanistic and structural studies of H373Q flavocytochrome b2: effects of mutating the active site base. Biochemistry. 2007;46:7844-51 pubmed
    ..The combination of an altered active site and the small kinetic isotope effects is consistent with the slowest step in turnover being a conformational change involving a conformation in which lactate is bound unproductively. ..
  4. Boubacar A, Pethe S, Mahy J, Lederer F. Flavocytochrome b2: reactivity of its flavin with molecular oxygen. Biochemistry. 2007;46:13080-8 pubmed
    ..An inspection of the available structures of family members does not provide a rationale for the difference between the oxidases and the electron transferases. ..
  5. Ookubo A, Hirasawa T, Yoshikawa K, Nagahisa K, Furusawa C, Shimizu H. Improvement of L-lactate production by CYB2 gene disruption in a recombinant Saccharomyces cerevisiae strain under low pH condition. Biosci Biotechnol Biochem. 2008;72:3063-6 pubmed
    ..Disruption of the CYB2 gene encoding L-lactate dehydrogenase improved the L-lactate production by S. cerevisiae under low pH condition.
  6. Mokranjac D, Sichting M, Popov Celeketic D, Mapa K, Gevorkyan Airapetov L, Zohary K, et al. Role of Tim50 in the transfer of precursor proteins from the outer to the inner membrane of mitochondria. Mol Biol Cell. 2009;20:1400-7 pubmed publisher
    ..This function of Tim50 depends on its association with TIM23. We conclude that the efficient transfer of precursors between TOM and TIM23 complexes requires the concerted action of Tim50 with Tim23. ..
  7. Bömer U, Meijer M, Guiard B, Dietmeier K, Pfanner N, Rassow J. The sorting route of cytochrome b2 branches from the general mitochondrial import pathway at the preprotein translocase of the inner membrane. J Biol Chem. 1997;272:30439-46 pubmed
    ..We suggest that the sorting signal of cytochrome b2 is recognized within the inner membrane in close vicinity to Tim23. ..
  8. Seytter T, Lottspeich F, Neupert W, Schwarz E. Mam33p, an oligomeric, acidic protein in the mitochondrial matrix of Saccharomyces cerevisiae is related to the human complement receptor gC1q-R. Yeast. 1998;14:303-10 pubmed
    ..Gene disruption did not result in a significant growth defect. Mam33p exhibits sequence similarity to gC1q-R, a human protein that has been implicated in the binding of complement factor C1q and kininogen. ..
  9. Grandier Vazeille X, Bathany K, Chaignepain S, Camougrand N, Manon S, Schmitter J. Yeast mitochondrial dehydrogenases are associated in a supramolecular complex. Biochemistry. 2001;40:9758-69 pubmed
    ..Nde1p and Nde2p, glycerol-3-phosphate dehydrogenase Gut2p, D- and L-lactate-dehydrogenases Dld1p and Cyb2p, the matrix-facing NADH-dehydrogenase Ndi1p, two probable flavoproteins YOR356Wp and YPR004Cp, four tricarboxylic ..

More Information


  1. Diep Le K, Lederer F, Golinelli Pimpaneau B. Structural evidence for the functional importance of the heme domain mobility in flavocytochrome b2. J Mol Biol. 2010;400:518-30 pubmed publisher
    Yeast flavocytochrome b(2) (Fcb2) is an L-lactate:cytochrome c oxidoreductase in the mitochondrial intermembrane space participating in cellular respiration...
  2. Lodi T, Ferrero I. Isolation of the DLD gene of Saccharomyces cerevisiae encoding the mitochondrial enzyme D-lactate ferricytochrome c oxidoreductase. Mol Gen Genet. 1993;238:315-24 pubmed
    ..1.2.3) encoded by the CYB2 gene, and D-lactate ferricytochrome c oxidoreductase (D-LCR) (EC, respectively...
  3. Sideris D, Petrakis N, Katrakili N, Mikropoulou D, Gallo A, Ciofi Baffoni S, et al. A novel intermembrane space-targeting signal docks cysteines onto Mia40 during mitochondrial oxidative folding. J Cell Biol. 2009;187:1007-22 pubmed publisher
  4. Popov Celeketic D, Waegemann K, Mapa K, Neupert W, Mokranjac D. Role of the import motor in insertion of transmembrane segments by the mitochondrial TIM23 complex. EMBO Rep. 2011;12:542-8 pubmed publisher
    ..Furthermore, our results suggest a role for the import motor in lateral insertion. Thus, the import motor is involved in ATP-dependent translocation and ATP-independent lateral insertion. ..