Gene Symbol: CWH41
Description: Cwh41p
Alias: DER7, GLS1, Cwh41p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Bickle M, Delley P, Schmidt A, Hall M. Cell wall integrity modulates RHO1 activity via the exchange factor ROM2. EMBO J. 1998;17:2235-45 pubmed
    ..We present evidence that cell wall defects activate RHO1. First, rot1, rot2, big1, cwh41, gas1 and fks1 mutations all confer cell wall defects and suppress tor2(ts)...
  2. Faridmoayer A, Scaman C. An improved purification procedure for soluble processing alpha-glucosidase I from Saccharomyces cerevisiae overexpressing CWH41. Protein Expr Purif. 2004;33:11-8 pubmed
    Processing alpha-glucosidase I, which is encoded by CWH41, regulates one of the key steps in asparagine-linked glycoprotein biosynthesis by cleaving the terminal alpha-1,2-linked glucose from Glc(3)Man(9)GlcNAc(2), the common ..
  3. Kurita T, Noda Y, Yoda K. Action of multiple endoplasmic reticulum chaperon-like proteins is required for proper folding and polarized localization of Kre6 protein essential in yeast cell wall ?-1,6-glucan synthesis. J Biol Chem. 2012;287:17415-24 pubmed publisher
    ..All mutants of the calnexin cycle member homologues (cwh41, rot2, kre5, and cne1) showed defects in ?-1,6-glucan synthesis, although the calnexin chaperon system is ..
  4. Schmelzle T, Helliwell S, Hall M. Yeast protein kinases and the RHO1 exchange factor TUS1 are novel components of the cell integrity pathway in yeast. Mol Cell Biol. 2002;22:1329-39 pubmed
    ..Furthermore, our findings suggest that the YPKs are a missing link between sphingolipid signaling and the cell integrity pathway. ..
  5. Hitt R, Wolf D. DER7, encoding alpha-glucosidase I is essential for degradation of malfolded glycoproteins of the endoplasmic reticulum. FEMS Yeast Res. 2004;4:815-20 pubmed
    ..Here we show that a previously isolated der mutant (der7-1) is defective in ERAD. We identify DER7 as the gene encoding N-glycan-processing alpha-glucosidase I (EC 3.2.1...
  6. Barker M, Rose D. Specificity of Processing ?-glucosidase I is guided by the substrate conformation: crystallographic and in silico studies. J Biol Chem. 2013;288:13563-74 pubmed publisher
    ..From these results, a model of substrate binding has been formulated, which is most likely conserved in mammalian GluI. ..
  7. Jiang B, Sheraton J, Ram A, Dijkgraaf G, Klis F, Bussey H. CWH41 encodes a novel endoplasmic reticulum membrane N-glycoprotein involved in beta 1,6-glucan assembly. J Bacteriol. 1996;178:1162-71 pubmed
    ..These results provide strong genetic evidence indicating that Cwh41p plays a functional role, possibly as a new synthetic component, in the assembly of cell wall beta 1,6-glucan.
  8. Romero P, Dijkgraaf G, Shahinian S, Herscovics A, Bussey H. The yeast CWH41 gene encodes glucosidase I. Glycobiology. 1997;7:997-1004 pubmed
    ..In the present work, it is shown that Cwh41p, a yeast endoplasmic reticulum protein previously identified as being required for normal cell wall beta-1,6-..
  9. Chen E, Kaiser C. LST8 negatively regulates amino acid biosynthesis as a component of the TOR pathway. J Cell Biol. 2003;161:333-47 pubmed
    ..Finally, the different effects of lst8 alleles on the activation of either the Rtg1/3p or Gln3p transcription factors reveal that these two pathways constitute distinct, genetically separable outputs of the Tor-Lst8 regulatory complex. ..

More Information


  1. Runge K, Robbins P. A new yeast mutation in the glucosylation steps of the asparagine-linked glycosylation pathway. Formation of a novel asparagine-linked oligosaccharide containing two glucose residues. J Biol Chem. 1986;261:15582-90 pubmed
    ..The yeast mutation gls1-1 lacks glucosidase I activity (Esmon, B., Esmon, P.C., and Schekman, R. (1984) J. Biol. Chem...
  2. Simons J, Ebersold M, Helenius A. Cell wall 1,6-beta-glucan synthesis in Saccharomyces cerevisiae depends on ER glucosidases I and II, and the molecular chaperone BiP/Kar2p. EMBO J. 1998;17:396-405 pubmed
    ..In S.cerevisiae, glucosidase II is encoded by the GLS2 gene, and glucosidase I, as suggested here, by the CWH41 gene...
  3. Abeijon C, Chen L. The role of glucosidase I (Cwh41p) in the biosynthesis of cell wall beta-1,6-glucan is indirect. Mol Biol Cell. 1998;9:2729-38 pubmed
    ..To distinguish between a direct or indirect role of Cwh41p in the biosynthesis of beta-1,6-glucan, we constructed a double mutant, alg5Delta (lacking dolichol-P-glucose ..
  4. Faridmoayer A, Scaman C. Truncations and functional carboxylic acid residues of yeast processing alpha-glucosidase I. Glycoconj J. 2007;24:429-37 pubmed
    Yeast alpha-glucosidase I (Cwh41p) encoded by CWH41 is an endoplasmic reticulum (ER) membrane-bound glycoprotein (833 residues), which plays an important role in the early steps of the N-glycosylation pathway...
  5. Shibuya A, Margulis N, Christiano R, Walther T, Barlowe C. The Erv41-Erv46 complex serves as a retrograde receptor to retrieve escaped ER proteins. J Cell Biol. 2015;208:197-209 pubmed publisher
    ..In cells lacking Erv41-Erv46 function, the ER enzyme glucosidase I (Gls1) was mislocalized and degraded in the vacuole...