Gene Symbol: CWC2
Description: Cwc2p
Alias: NTC40, Cwc2p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. McGrail J, Krause A, O Keefe R. The RNA binding protein Cwc2 interacts directly with the U6 snRNA to link the nineteen complex to the spliceosome during pre-mRNA splicing. Nucleic Acids Res. 2009;37:4205-17 pubmed publisher
    ..A candidate for linking the NTC to the snRNAs is the NTC protein Cwc2, which contains motifs known to bind RNA, a zinc finger and RNA recognition motif (RRM)...
  2. Schmitzová J, Rasche N, Dybkov O, Kramer K, Fabrizio P, Urlaub H, et al. Crystal structure of Cwc2 reveals a novel architecture of a multipartite RNA-binding protein. EMBO J. 2012;31:2222-34 pubmed publisher
    The yeast splicing factor Cwc2 contacts several catalytically important RNA elements in the active spliceosome, suggesting that Cwc2 is involved in determining their spatial arrangement at the spliceosome's catalytic centre...
  3. Ohi M, Gould K. Characterization of interactions among the Cef1p-Prp19p-associated splicing complex. RNA. 2002;8:798-815 pubmed
    ..Our data indicate that Cwc2p, Prp46p, Clf1p, and Syf1p most likely represent Ntc40p, Ntc50p, Ntc77p, and Ntc90p, respectively...
  4. Rasche N, Dybkov O, Schmitzová J, Akyildiz B, Fabrizio P, Luhrmann R. Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre. EMBO J. 2012;31:1591-604 pubmed publisher
    ..We show that the S. cerevisiae Cwc2 protein functions prior to step 1 of splicing, and it is not required for the Prp2-mediated spliceosome remodelling ..
  5. Lu P, Lu G, Yan C, Wang L, Li W, Yin P. Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition. Biochem J. 2012;441:591-7 pubmed publisher
    ..Only one component of the NTC, Cwc2, is capable of binding RNA. In the present study we report the 1.9 Å (1 Å=0...
  6. Hogg R, de Almeida R, Ruckshanthi J, O Keefe R. Remodeling of U2-U6 snRNA helix I during pre-mRNA splicing by Prp16 and the NineTeen Complex protein Cwc2. Nucleic Acids Res. 2014;42:8008-23 pubmed publisher
    ..How Prp16 destabilizes U2-U6 helix I is not clear. We show that the NineTeen Complex (NTC) protein Cwc2 displays genetic interactions with the U6 ACAGAGA, the U6 internal stem loop (ISL) and the U2-U6 helix I, all RNA ..
  7. Warkocki Z, Schneider C, Mozaffari Jovin S, Schmitzová J, Höbartner C, Fabrizio P, et al. The G-patch protein Spp2 couples the spliceosome-stimulated ATPase activity of the DEAH-box protein Prp2 to catalytic activation of the spliceosome. Genes Dev. 2015;29:94-107 pubmed publisher
    ..Our data suggest that Spp2 plays a major role in coupling Prp2's ATPase activity to remodeling of the spliceosome into a catalytically active machine. ..
  8. Tsai W, Chow Y, Chen H, Huang K, Hong R, Jan S, et al. Cef1p is a component of the Prp19p-associated complex and essential for pre-mRNA splicing. J Biol Chem. 1999;274:9455-62 pubmed
  9. Gahura O, Abrhámová K, Skruzny M, Valentová A, Munzarová V, Folk P, et al. Prp45 affects Prp22 partition in spliceosomal complexes and splicing efficiency of non-consensus substrates. J Cell Biochem. 2009;106:139-51 pubmed publisher
    ..b>Cwc2-associated spliceosomal complexes purified from prp45(1-169) cells showed decreased stoichiometry of Prp22, ..

More Information


  1. Vander Kooi C, Ren L, Xu P, Ohi M, Gould K, Chazin W. The Prp19 WD40 domain contains a conserved protein interaction region essential for its function. Structure. 2010;18:584-93 pubmed publisher
    ..highly conserved surface centered around blade five that is required for the physical interaction between Prp19 and Cwc2, another essential splicing factor. This region is found to be required for Prp19 function and yeast viability...
  2. Vincent K, Wang Q, Jay S, Hobbs K, Rymond B. Genetic interactions with CLF1 identify additional pre-mRNA splicing factors and a link between activators of yeast vesicular transport and splicing. Genetics. 2003;164:895-907 pubmed
    ..Prp22, and Syf2 splicing factors and four proteins without established contribution to splicing (Bud13, Cet1, Cwc2, and Rds3). Each synthetic lethal with clf1Delta2 (slc) mutant is splicing defective in a wild-type CLF1 background...