Gene Symbol: CUS1
Description: U2 snRNP complex subunit CUS1
Alias: U2 snRNP complex subunit CUS1
Species: Saccharomyces cerevisiae S288c
Wells S, Neville M, Haynes M, Wang J, Igel H, Ares M. CUS1, a suppressor of cold-sensitive U2 snRNA mutations, is a novel yeast splicing factor homologous to human SAP 145. Genes Dev. 1996;10:220-32 pubmed
..The screen identified three dominant suppressor genes, one of which, CUS1-54, encodes an essential splicing protein required for U2 snRNP addition to the spliceosome...
Yan D, Ares M. Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits. Mol Cell Biol. 1996;16:818-28 pubmed
..Lethal mutations in the region are suppressed by Cus1-54p, a mutant yeast splicing factor homologous to a mammalian SF3b subunit...
Wang Q, He J, Lynn B, Rymond B. Interactions of the yeast SF3b splicing factor. Mol Cell Biol. 2005;25:10745-54 pubmed
..These data establish the concordance of yeast and mammalian SF3b complexes, implicate accessory splicing factors in U2 snRNP function, and support SF3b contribution from early pre-mRNP recognition to late steps in splicing. ..
Dziembowski A, Ventura A, Rutz B, Caspary F, Faux C, Halgand F, et al
. Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing. EMBO J. 2004;23:4847-56 pubmed
..The corresponding complex was named pre-mRNA REtention and Splicing (RES). The presence of RES subunit homologues in numerous eukaryotes suggests that its function is evolutionarily conserved. ..
Igel H, Wells S, Perriman R, Ares M. Conservation of structure and subunit interactions in yeast homologues of splicing factor 3b (SF3b) subunits. RNA. 1998;4:1-10 pubmed
..contains two RNA recognition motifs (RRMs) and binds another SF3b subunit called SAP 145, whose yeast homologue is CUS1. Here we show that the predicted yeast open reading frame YOR319w (HSH49) encodes an essential yeast splicing ..
Pauling M, McPheeters D, Ares M. Functional Cus1p is found with Hsh155p in a multiprotein splicing factor associated with U2 snRNA. Mol Cell Biol. 2000;20:2176-85 pubmed
..and reconstitution of heat-inactivated splicing extracts from strains carrying a temperature-sensitive allele of CUS1 indicate that Cus1p and Hsh155p reside in a functional, high-salt-stable complex that is salt-dissociable from U2 ..
Hoskins A, Friedman L, Gallagher S, Crawford D, Anderson E, Wombacher R, et al
. Ordered and dynamic assembly of single spliceosomes. Science. 2011;331:1289-95 pubmed publisher
..This experimental strategy should prove widely useful for mechanistic analysis of other macromolecular machines in environments approaching the complexity of living cells. ..
Carrocci T, Zoerner D, Paulson J, Hoskins A. SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast. Nucleic Acids Res. 2017;45:4837-4852 pubmed publisher
..We propose that SF3b1 functions to stabilize weak U2/BS duplexes to drive spliceosome assembly and splicing. ..
van Roon A, Oubridge C, Obayashi E, Sposito B, Newman A, Seraphin B, et al
. Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p-binding domain. RNA. 2017;23:968-981 pubmed publisher
..The structures show that the Cus1 fragment binds to the ?-helical surface of Hsh49p RRM1, opposite the four-stranded ?-sheet, leaving the canonical ..