Gene Symbol: CUE1
Description: Cue1p
Alias: KIS4, Cue1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Carvalho P, Goder V, Rapoport T. Distinct ubiquitin-ligase complexes define convergent pathways for the degradation of ER proteins. Cell. 2006;126:361-73 pubmed
    ..All three pathways converge at the Cdc48p ATPase complex. These results lead to a unifying concept for ERAD that may also apply to mammalian cells. ..
  2. Auld K, Hitchcock A, Doherty H, Frietze S, Huang L, Silver P. The conserved ATPase Get3/Arr4 modulates the activity of membrane-associated proteins in Saccharomyces cerevisiae. Genetics. 2006;174:215-27 pubmed
    ..In combination, our characterization of GET3 genetic and biochemical interactions with NPL4, GET1, and GET2 implicates Get3 in multiple membrane-dependent pathways. ..
  3. Bagola K, von Delbrück M, Dittmar G, Scheffner M, Ziv I, Glickman M, et al. Ubiquitin binding by a CUE domain regulates ubiquitin chain formation by ERAD E3 ligases. Mol Cell. 2013;50:528-39 pubmed publisher
    ..Interestingly, so far no significant ubiquitin binding has been observed for the CUE domain of yeast Cue1p. Cue1p is receptor and activator of the ubiquitin-conjugating enzyme Ubc7p...
  4. Ng W, Sergeyenko T, Zeng N, Brown J, Römisch K. Characterization of the proteasome interaction with the Sec61 channel in the endoplasmic reticulum. J Cell Sci. 2007;120:682-91 pubmed
    ..Mutations in the ATP-binding sites of individual Rpt proteins all reduced the affinity of 19S complexes for the ER, suggesting that the 19S base in the ATP-bound conformation docks at the Sec61 channel. ..
  5. Nakatsukasa K, Okada S, Umebayashi K, Fukuda R, Nishikawa S, Endo T. Roles of O-mannosylation of aberrant proteins in reduction of the load for endoplasmic reticulum chaperones in yeast. J Biol Chem. 2004;279:49762-72 pubmed
    ..O-Mannosylation may therefore function as a fail-safe mechanism for the ERAD by solubilizing the aberrant proteins that overflowed from the ERAD pathway and reducing the load for ER chaperones. ..
  6. Plemper R, Bordallo J, Deak P, Taxis C, Hitt R, Wolf D. Genetic interactions of Hrd3p and Der3p/Hrd1p with Sec61p suggest a retro-translocation complex mediating protein transport for ER degradation. J Cell Sci. 1999;112 ( Pt 22):4123-34 pubmed
    ..The retro-translocon seems to be build up at least by the Sec61 pore, Der3p/Hrd1p and Hrd3p and mediates both retrograde transport and ubiquitination of substrate molecules. ..
  7. McBratney S, Winey M. Mutant membrane protein of the budding yeast spindle pole body is targeted to the endoplasmic reticulum degradation pathway. Genetics. 2002;162:567-78 pubmed
    ..b>Cue1p has been shown to recruit the soluble ubiquitin-conjugating enzyme, Ubc7p, to the cytoplasmic face of the ER ..
  8. Neuber O, Jarosch E, Volkwein C, Walter J, Sommer T. Ubx2 links the Cdc48 complex to ER-associated protein degradation. Nat Cell Biol. 2005;7:993-8 pubmed
    ..Thus, a complex comprising the AAA ATPase, a ubiquitin ligase and the recruitment factor Ubx2 has a central role in ER-associated proteolysis. ..
  9. Kostova Z, Mariano J, Scholz S, Koenig C, Weissman A. A Ubc7p-binding domain in Cue1p activates ER-associated protein degradation. J Cell Sci. 2009;122:1374-81 pubmed publisher
    b>Cue1p is an N-terminally anchored endoplasmic reticulum (ER) protein essential for the activity of the two major yeast RING finger ubiquitin ligases (E3s) implicated in ER-associated degradation (ERAD)...

More Information


  1. Wang S, Ng D. Evasion of endoplasmic reticulum surveillance makes Wsc1p an obligate substrate of Golgi quality control. Mol Biol Cell. 2010;21:1153-65 pubmed publisher
    ..By evading ERAD through these passive and active mechanisms, Wsc1p is fully dependent on the post-ER system for its quality control. ..
  2. Ravid T, Kreft S, Hochstrasser M. Membrane and soluble substrates of the Doa10 ubiquitin ligase are degraded by distinct pathways. EMBO J. 2006;25:533-43 pubmed
    ..For all these substrates, Doa10 requires two Ub-conjugating enzymes, Ubc6 and Ubc7, as well as the Ubc7 cofactor Cue1. Based on a novel genomic screen of a comprehensive gene deletion library and other data, these four proteins ..
  3. Ravid T, Hochstrasser M. Autoregulation of an E2 enzyme by ubiquitin-chain assembly on its catalytic residue. Nat Cell Biol. 2007;9:422-7 pubmed
    ..we find that Ubc7, a yeast E2, can itself undergo degradation when its levels exceed that of its binding partner Cue1, a transmembrane protein that tethers Ubc7 to the endoplasmic reticulum...
  4. von Delbrück M, Kniss A, Rogov V, Pluska L, Bagola K, Lohr F, et al. The CUE Domain of Cue1 Aligns Growing Ubiquitin Chains with Ubc7 for Rapid Elongation. Mol Cell. 2016;62:918-928 pubmed publisher
    ..Only recently has ubiquitin binding activity emerged as an important factor in chain formation. The Ubc7 activator Cue1 carries a ubiquitin binding CUE domain that substantially stimulates K48-linked polyubiquitination mediated by Ubc7...
  5. Loertscher J, Larson L, Matson C, Parrish M, Felthauser A, Sturm A, et al. Endoplasmic reticulum-associated degradation is required for cold adaptation and regulation of sterol biosynthesis in the yeast Saccharomyces cerevisiae. Eukaryot Cell. 2006;5:712-22 pubmed
    ..We have discovered that the ERAD proteins Ubc7 (Qri8), Cue1, and Doa10 (Ssm4) are required for growth of yeast that express high levels of the sterol biosynthetic enzyme, 3-..
  6. Metzger M, Liang Y, Das R, Mariano J, Li S, Li J, et al. A structurally unique E2-binding domain activates ubiquitination by the ERAD E2, Ubc7p, through multiple mechanisms. Mol Cell. 2013;50:516-27 pubmed publisher
    b>Cue1p is an integral component of yeast endoplasmic reticulum (ER)-associated degradation (ERAD) ubiquitin ligase (E3) complexes...
  7. Hitchcock A, Auld K, Gygi S, Silver P. A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery. Proc Natl Acad Sci U S A. 2003;100:12735-40 pubmed
    ..Interestingly, we also identified novel membrane-bound transcription factors that may be subject to ubiquitin/proteasome-mediated cleavage and activation at the ER membrane. ..
  8. Bazirgan O, Hampton R. Cue1p is an activator of Ubc7p E2 activity in vitro and in vivo. J Biol Chem. 2008;283:12797-810 pubmed publisher
    ..Ubc7p only functions in ERAD if bound to the ER surface by Cue1p, a membrane-anchored ER protein...
  9. Biederer T, Volkwein C, Sommer T. Role of Cue1p in ubiquitination and degradation at the ER surface. Science. 1997;278:1806-9 pubmed
    ..Here, a membrane-bound component of the ubiquitin system, Cue1p, was identified. It was shown to recruit the soluble ubiquitin-conjugating enzyme Ubc7p to the ER membrane...
  10. Cohen I, Wiener R, Reiss Y, Ravid T. Distinct activation of an E2 ubiquitin-conjugating enzyme by its cognate E3 ligases. Proc Natl Acad Sci U S A. 2015;112:E625-32 pubmed publisher