Gene Symbol: CTF18
Description: Ctf18p
Alias: CHL12, Ctf18p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Hanna J, Kroll E, Lundblad V, Spencer F. Saccharomyces cerevisiae CTF18 and CTF4 are required for sister chromatid cohesion. Mol Cell Biol. 2001;21:3144-58 pubmed
    ..We find that Ctf18p, an RFC1-like protein, directly interacts with Rfc2p, Rfc3p, Rfc4p, and Rfc5p...
  2. Bylund G, Majka J, Burgers P. Overproduction and purification of RFC-related clamp loaders and PCNA-related clamps from Saccharomyces cerevisiae. Methods Enzymol. 2006;409:1-11 pubmed
    ..the large Rfc1 subunit by a pathway-specific alternative large subunit, Rad24 for the DNA damage checkpoint, Ctf18 for the establishment of sister chromatid cohesion, and Elg1 for a general function in chromosome stability...
  3. Hiraga S, Robertson E, Donaldson A. The Ctf18 RFC-like complex positions yeast telomeres but does not specify their replication time. EMBO J. 2006;25:1505-14 pubmed
    Chromosome ends in Saccharomyces cerevisiae are positioned in clusters at the nuclear rim. We report that Ctf18, Ctf8, and Dcc1, the subunits of a Replication Factor C (RFC)-like complex, are essential for the perinuclear positioning of ..
  4. Skibbens R, Corson L, Koshland D, Hieter P. Ctf7p is essential for sister chromatid cohesion and links mitotic chromosome structure to the DNA replication machinery. Genes Dev. 1999;13:307-19 pubmed
    ..In addition, ctf7 genetically interacts with DNA metabolism mutations pol30 (PCNA) and ctf18 (an RF-C like protein) and ctf7 temperature sensitivity and chromosome loss are rescued by high levels of POL30...
  5. Xu H, Boone C, Brown G. Genetic dissection of parallel sister-chromatid cohesion pathways. Genetics. 2007;176:1417-29 pubmed
    ..data defined two cohesion pathways, one containing CSM3, TOF1, CTF4, and CHL1, and the second containing MRC1, CTF18, CTF8, and DCC1...
  6. Bylund G, Burgers P. Replication protein A-directed unloading of PCNA by the Ctf18 cohesion establishment complex. Mol Cell Biol. 2005;25:5445-55 pubmed
    ..Unloading requires ATP hydrolysis. This seven-subunit Ctf18-RFC complex consists of the four small subunits of RFC, together with Ctf18, Dcc1, and Ctf8...
  7. Moriel Carretero M, Aguilera A. A postincision-deficient TFIIH causes replication fork breakage and uncovers alternative Rad51- or Pol32-mediated restart mechanisms. Mol Cell. 2010;37:690-701 pubmed publisher
    ..Our results define the genetic and molecular hallmarks of replication fork breakage and restart and bring insights to understand specific NER-related human syndromes. ..
  8. Ogiwara H, Ohuchi T, Ui A, Tada S, Enomoto T, Seki M. Ctf18 is required for homologous recombination-mediated double-strand break repair. Nucleic Acids Res. 2007;35:4989-5000 pubmed
    ..In this study, we investigated whether Ctf18, a factor implicated in the establishment of sister chromatid cohesion, is involved in DSB repair in budding yeast...
  9. Parnas O, Zipin Roitman A, Mazor Y, Liefshitz B, Ben Aroya S, Kupiec M. The ELG1 clamp loader plays a role in sister chromatid cohesion. PLoS ONE. 2009;4:e5497 pubmed publisher
    ..Two additional RLCs exist in yeast: in one of them the large subunit is Ctf18, and in the other, Rad24. Ctf18 has been characterized as the RLC that functions in sister chromatid cohesion...

More Information


  1. Kenna M, Skibbens R. Mechanical link between cohesion establishment and DNA replication: Ctf7p/Eco1p, a cohesion establishment factor, associates with three different replication factor C complexes. Mol Cell Biol. 2003;23:2999-3007 pubmed
    ..Chl12p/Ctf18p combines with Rfc2p to Rfc5p to form one of three independent RFC complexes...
  2. Mayer M, Gygi S, Aebersold R, Hieter P. Identification of RFC(Ctf18p, Ctf8p, Dcc1p): an alternative RFC complex required for sister chromatid cohesion in S. cerevisiae. Mol Cell. 2001;7:959-70 pubmed
    We have identified and characterized an alternative RFC complex RFC(Ctf18p, Ctf8p, Dcc1p) that is required for sister chromatid cohesion and faithful chromosome transmission...
  3. Lengronne A, McIntyre J, Katou Y, Kanoh Y, Hopfner K, Shirahige K, et al. Establishment of sister chromatid cohesion at the S. cerevisiae replication fork. Mol Cell. 2006;23:787-99 pubmed
    ..Here we show that three proteins required for sister chromatid cohesion, Eco1, Ctf4, and Ctf18, are found at, and Ctf4 travels along chromosomes with, replication forks...
  4. Naiki T, Kondo T, Nakada D, Matsumoto K, Sugimoto K. Chl12 (Ctf18) forms a novel replication factor C-related complex and functions redundantly with Rad24 in the DNA replication checkpoint pathway. Mol Cell Biol. 2001;21:5838-45 pubmed
    ..CHL12 (also called CTF18) encodes a protein that is structurally related to the Rad24 and RFC proteins...
  5. Crabbe L, Thomas A, Pantesco V, de Vos J, Pasero P, Lengronne A. Analysis of replication profiles reveals key role of RFC-Ctf18 in yeast replication stress response. Nat Struct Mol Biol. 2010;17:1391-7 pubmed publisher
    ..In contrast, we found that RFC(Ctf18) is essential for the Mrc1-dependent activation of Rad53 and for the maintenance of paused forks...
  6. Kanellis P, Agyei R, Durocher D. Elg1 forms an alternative PCNA-interacting RFC complex required to maintain genome stability. Curr Biol. 2003;13:1583-95 pubmed
    ..Elg1, a conserved but uncharacterized homolog of the large RFC subunit Rfc1 and the alternative RFC subunits Ctf18/Chl12 and Rad24...
  7. Pike B, Heierhorst J. Mdt1 facilitates efficient repair of blocked DNA double-strand breaks and recombinational maintenance of telomeres. Mol Cell Biol. 2007;27:6532-45 pubmed
    ..synthetic growth defects with a deletion of the recombination facilitator and telomere-positioning factor gene CTF18 already in the absence of exogenous DNA damage...
  8. Gómez González B, Felipe Abrio I, Aguilera A. The S-phase checkpoint is required to respond to R-loops accumulated in THO mutants. Mol Cell Biol. 2009;29:5203-13 pubmed publisher
    ..In light of these results, we propose a model in which R-loop-mediated recombination is explained by template switching. ..
  9. Putnam C, Hayes T, Kolodner R. Specific pathways prevent duplication-mediated genome rearrangements. Nature. 2009;460:984-9 pubmed publisher
    ..This explains how extensive genome instability is prevented in eukaryotic cells whose genomes contain numerous divergent repeated sequences...
  10. Keogh M, Kim J, Downey M, Fillingham J, Chowdhury D, Harrison J, et al. A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery. Nature. 2006;439:497-501 pubmed
    ..The dephosphorylation of gammaH2AX by the HTP-C is necessary for efficient recovery from the DNA damage checkpoint. ..
  11. Gao H, Moss D, Parke C, Tatum D, Lustig A. The Ctf18RFC clamp loader is essential for telomere stability in telomerase-negative and mre11 mutant alleles. PLoS ONE. 2014;9:e88633 pubmed publisher
    ..We have investigated the variant clamp loader Ctf18 RFC (Replication Factor C)...
  12. McLellan J, O Neil N, Tarailo S, Stoepel J, Bryan J, Rose A, et al. Synthetic lethal genetic interactions that decrease somatic cell proliferation in Caenorhabditis elegans identify the alternative RFC CTF18 as a candidate cancer drug target. Mol Biol Cell. 2009;20:5306-13 pubmed publisher
    ..SL interactions between members of the cohesin complex and CTF4, RAD27, and components of the alternative RFC(CTF18) complex. The genetic interactions tested are highly conserved between S. cerevisiae and C...
  13. Litwin I, Bakowski T, Maciaszczyk Dziubinska E, Wysocki R. The LSH/HELLS homolog Irc5 contributes to cohesin association with chromatin in yeast. Nucleic Acids Res. 2017;45:6404-6416 pubmed publisher
    ..Our results suggest that Irc5 is an auxiliary factor that is involved in cohesin association with chromatin. ..
  14. Myung K, Smith S, Kolodner R. Mitotic checkpoint function in the formation of gross chromosomal rearrangements in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2004;101:15980-5 pubmed
    ..These data suggest that functional mitotic checkpoints can play a role in the formation of genome rearrangements. ..
  15. Jessulat M, Alamgir M, Salsali H, Greenblatt J, Xu J, Golshani A. Interacting proteins Rtt109 and Vps75 affect the efficiency of non-homologous end-joining in Saccharomyces cerevisiae. Arch Biochem Biophys. 2008;469:157-64 pubmed
    ..We propose that one function of the Rtt109-Vps75 interacting protein pair is to affect the efficiency of NHEJ in yeast. Vps75 but not Rtt109 also seem to have an effect on the efficiency of DSB repair using homologous recombination. ..
  16. Okimoto H, Tanaka S, Araki H, Ohashi E, Tsurimoto T. Conserved interaction of Ctf18-RFC with DNA polymerase ? is critical for maintenance of genome stability in Saccharomyces cerevisiae. Genes Cells. 2016;21:482-91 pubmed publisher
    Human Ctf18-RFC, a PCNA loader complex, interacts with DNA polymerase ? (Pol?) through a structure formed by the Ctf18, Dcc1 and Ctf8 subunits...
  17. Esta A, Ma E, Dupaigne P, Maloisel L, Guerois R, Le Cam E, et al. Rad52 sumoylation prevents the toxicity of unproductive Rad51 filaments independently of the anti-recombinase Srs2. PLoS Genet. 2013;9:e1003833 pubmed publisher
    ..This conclusion is strengthened by the finding that Rad52 is often associated with complete Rad51 filaments in vitro...
  18. Haas J, Lemoncelli A, Morozov C, Franke K, Dominder J, Antoniacci L. Physical links between the nuclear envelope protein Mps3, three alternate replication factor C complexes, and a variant histone in Saccharomyces cerevisiae. DNA Cell Biol. 2012;31:917-24 pubmed publisher
    ..Budding yeast contain four alternate RFC complexes which play partially redundant roles. Rfc1, Ctf18, Rad24, and Elg1 are all large subunits that bind, in a mutually exclusive fashion to RFC 2-5 small subunits...
  19. Reha Krantz L, Siddique M, Murphy K, Tam A, O Carroll M, Lou S, et al. Drug-sensitive DNA polymerase ? reveals a role for mismatch repair in checkpoint activation in yeast. Genetics. 2011;189:1211-24 pubmed publisher
  20. Díaz de la Loza M, Gallardo M, García Rubio M, Izquierdo A, Herrero E, Aguilera A, et al. Zim17/Tim15 links mitochondrial iron-sulfur cluster biosynthesis to nuclear genome stability. Nucleic Acids Res. 2011;39:6002-15 pubmed publisher
    ..We propose that compromised ribosome biosynthesis and cell-cycle progression are interconnected, together contributing to replicative stress and nuclear genome instability in zim17? mutants. ..
  21. Kubota T, Hiraga S, Yamada K, Lamond A, Donaldson A. Quantitative proteomic analysis of chromatin reveals that Ctf18 acts in the DNA replication checkpoint. Mol Cell Proteomics. 2011;10:M110.005561 pubmed publisher
    Yeast cells lacking Ctf18, the major subunit of an alternative Replication Factor C complex, have multiple problems with genome stability...
  22. Gellon L, Razidlo D, Gleeson O, Verra L, Schulz D, Lahue R, et al. New functions of Ctf18-RFC in preserving genome stability outside its role in sister chromatid cohesion. PLoS Genet. 2011;7:e1001298 pubmed publisher
    ..we show that CAG/CTG tracts are stabilized in Saccharomyces cerevisiae by the alternative clamp loader/unloader Ctf18-Dcc1-Ctf8-RFC complex (Ctf18-RFC)...
  23. Ohuchi T, Seki M, Kugou K, Tada S, Ohta K, Enomoto T. Accumulation of sumoylated Rad52 in checkpoint mutants perturbed in DNA replication. DNA Repair (Amst). 2009;8:690-6 pubmed publisher
    ..Double mutation of RAD51 and RAD53 exhibited the similar levels of Rad52 sumoylation to RAD53 single mutation. The significance and regulation mechanism of Rad52 sumoylation by checkpoint pathways will be discussed. ..
  24. Ma L, Zhai Y, Feng D, Chan T, Lu Y, Fu X, et al. Identification of novel factors involved in or regulating initiation of DNA replication by a genome-wide phenotypic screen in Saccharomyces cerevisiae. Cell Cycle. 2010;9:4399-410 pubmed
    ..in previously known initiation proteins and identified several novel factors, including Ctf1p Ctf3p, Ctf4p, Ctf18p, Adk1p and Cdc60p, whose mutants lose plasmid containing a single replication origin at high rates but lose ..
  25. Warren C, Brady D, Johnston R, Hanna J, Hardwick K, Spencer F. Distinct chromosome segregation roles for spindle checkpoint proteins. Mol Biol Cell. 2002;13:3029-41 pubmed
    ..Analysis of this activity indicates that the Bub3p-binding domain of Bub1p contributes to this phenotype through disruption of checkpoint activity as well as through introduction of kinetochore or spindle damage. ..
  26. Borges V, Smith D, Whitehouse I, Uhlmann F. An Eco1-independent sister chromatid cohesion establishment pathway in S. cerevisiae. Chromosoma. 2013;122:121-34 pubmed publisher
    ..In addition to Eco1, several other factors contribute to cohesion establishment, including Ctf4, Ctf18, Tof1, Csm3, Chl1 and Mrc1, but little is known about their roles...
  27. Campbell C, Desai A. Tension sensing by Aurora B kinase is independent of survivin-based centromere localization. Nature. 2013;497:118-21 pubmed publisher
    ..These results suggest that activation of Aurora B kinase by clustering either on chromatin or on microtubules is sufficient for chromosome biorientation...
  28. Kadyrova L, Mertz T, Zhang Y, Northam M, Sheng Z, Lobachev K, et al. A reversible histone H3 acetylation cooperates with mismatch repair and replicative polymerases in maintaining genome stability. PLoS Genet. 2013;9:e1003899 pubmed publisher
    ..Our results suggest that cyclic acetylation and deacetylation of chromatin contribute to replication fidelity and play important roles in the protection of nuclear DNA from diverse spontaneous mutations. ..
  29. Formosa T, Eriksson P, Wittmeyer J, Ginn J, Yu Y, Stillman D. Spt16-Pob3 and the HMG protein Nhp6 combine to form the nucleosome-binding factor SPN. EMBO J. 2001;20:3506-17 pubmed
    ..These complexes have altered electrophoretic mobility and a distinct pattern of enhanced sensitivity to DNase I. These results suggest that Spt16-Pob3 and Nhp6 cooperate to function as a novel nucleosome reorganizing factor. ..
  30. García Rodríguez L, De Piccoli G, Marchesi V, Jones R, Edmondson R, Labib K. A conserved Polϵ binding module in Ctf18-RFC is required for S-phase checkpoint activation downstream of Mec1. Nucleic Acids Res. 2015;43:8830-8 pubmed publisher
    ..Work with budding yeast showed that the 'alternative clamp loader' known as Ctf18-RFC acts by an unknown mechanism to activate the checkpoint kinase Rad53, which then mediates much of the ..
  31. Zhang W, Durocher D. De novo telomere formation is suppressed by the Mec1-dependent inhibition of Cdc13 accumulation at DNA breaks. Genes Dev. 2010;24:502-15 pubmed publisher
    ..These studies therefore identify a mechanism by which the ATR family of kinases enforces genome integrity, and a process that underscores the contribution of Cdc13 to the fate of DNA ends. ..
  32. Villa F, Simon A, Ortiz Bazan M, Kilkenny M, Wirthensohn D, Wightman M, et al. Ctf4 Is a Hub in the Eukaryotic Replisome that Links Multiple CIP-Box Proteins to the CMG Helicase. Mol Cell. 2016;63:385-96 pubmed publisher
    ..Most strikingly, Ctf4-dependent recruitment of CIP-box proteins couples other processes to DNA synthesis, including rDNA copy-number regulation. ..
  33. Schlesinger M, Formosa T. POB3 is required for both transcription and replication in the yeast Saccharomyces cerevisiae. Genetics. 2000;155:1593-606 pubmed
    ..between pob3 mutations and the genes encoding several DNA replication factors, including POL1, CTF4, DNA2, and CHL12. pob3 alleles caused sensitivity to the ribonucleotide reductase inhibitor hydroxyurea, indicating a defect in a ..