CSE4

Summary

Gene Symbol: CSE4
Description: centromeric DNA-binding histone H3-like protein CSE4
Alias: CSL2, centromeric DNA-binding histone H3-like protein CSE4
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Camahort R, Shivaraju M, Mattingly M, Li B, Nakanishi S, Zhu D, et al. Cse4 is part of an octameric nucleosome in budding yeast. Mol Cell. 2009;35:794-805 pubmed publisher
    The budding yeast CenH3 histone variant Cse4 localizes to centromeric nucleosomes and is required for kinetochore assembly and chromosome segregation. The exact composition of centromeric Cse4-containing nucleosomes is a subject of debate...
  2. Ortiz J, Stemmann O, Rank S, Lechner J. A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore. Genes Dev. 1999;13:1140-55 pubmed
    ..the CDE III element of the centromere DNA, with further components of the budding yeast centromere, Cbf1, Mif2, and Cse4. We demonstrate that the CDE III element is essential and sufficient to localize the established protein network to ..
  3. Glowczewski L, Yang P, Kalashnikova T, Santisteban M, Smith M. Histone-histone interactions and centromere function. Mol Cell Biol. 2000;20:5700-11 pubmed
    ..this model, we targeted random mutations to the Cse4p histone fold domain and isolated three temperature-sensitive cse4 alleles in an unbiased genetic screen. Two of the cse4 alleles contain mutations at the Cse4p-H4 interface...
  4. Dechassa M, Wyns K, Luger K. Scm3 deposits a (Cse4-H4)2 tetramer onto DNA through a Cse4-H4 dimer intermediate. Nucleic Acids Res. 2014;42:5532-42 pubmed publisher
    ..In budding yeast, the centromere-specific histone H3 variant is Cse4, and the histone chaperone Scm3 functions as a Cse4-specific nucleosome assembly factor...
  5. Ohkuni K, Abdulle R, Kitagawa K. Degradation of centromeric histone H3 variant Cse4 requires the Fpr3 peptidyl-prolyl Cis-Trans isomerase. Genetics. 2014;196:1041-5 pubmed publisher
    The centromeric histone H3 variant Cse4 in Saccharomyces cerevisiae is polyubiquitylated and degraded in a proteasome-dependent manner. We report here that the proline isomerase Fpr3 regulates Cse4 proteolysis...
  6. Mishra P, Au W, Choy J, Kuich P, Baker R, Foltz D, et al. Misregulation of Scm3p/HJURP causes chromosome instability in Saccharomyces cerevisiae and human cells. PLoS Genet. 2011;7:e1002303 pubmed publisher
    ..Overexpression of CSE4 or histone H4 suppressed chromosome loss and restored levels of Cse4p at centromeres in GALSCM3 strains...
  7. London N, Ceto S, Ranish J, Biggins S. Phosphoregulation of Spc105 by Mps1 and PP1 regulates Bub1 localization to kinetochores. Curr Biol. 2012;22:900-6 pubmed publisher
    ..Together, these data identify Spc105 as a key target of the Mps1 kinase and show that the opposing activities of Mps1 and PP1 regulate the kinetochore localization of the Bub1 protein...
  8. Collins K, Furuyama S, Biggins S. Proteolysis contributes to the exclusive centromere localization of the yeast Cse4/CENP-A histone H3 variant. Curr Biol. 2004;14:1968-72 pubmed
    ..Here, we show that the levels of the budding yeast CenH3, Cse4, are regulated by ubiquitin-proteasome-mediated proteolysis...
  9. Shang C, Hazbun T, Cheeseman I, Aranda J, Fields S, Drubin D, et al. Kinetochore protein interactions and their regulation by the Aurora kinase Ipl1p. Mol Biol Cell. 2003;14:3342-55 pubmed
    ..Strikingly, we found that the Dam1p-Ndc80p and Dam1p-Spc34p interactions were weakened by mutations mimicking phosphorylation at Ipl1p sites, allowing us to formulate a model for the effects of phosphoregulation on kinetochore function. ..

More Information

Publications67

  1. Bock L, Pagliuca C, Kobayashi N, Grove R, Oku Y, Shrestha K, et al. Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore. Nat Cell Biol. 2012;14:614-24 pubmed publisher
    ..Cnn1 regulates KMN activity in a spatiotemporal manner by inhibiting the interaction between its complexes. Cnn1 activity peaks in anaphase and is driven by the Cdc28, Mps1 and Ipl1 kinases. ..
  2. Keith K, Fitzgerald Hayes M. CSE4 genetically interacts with the Saccharomyces cerevisiae centromere DNA elements CDE I and CDE II but not CDE III. Implications for the path of the centromere dna around a cse4p variant nucleosome. Genetics. 2000;156:973-81 pubmed
    ..the genetic interactions between Cse4p and centromere DNA, we measured the chromosome loss rates exhibited by cse4 cen3 double-mutant cells that express mutant Cse4 proteins and carry chromosomes containing mutant centromere DNA (..
  3. Xiao H, Mizuguchi G, Wisniewski J, Huang Y, Wei D, Wu C. Nonhistone Scm3 binds to AT-rich DNA to organize atypical centromeric nucleosome of budding yeast. Mol Cell. 2011;43:369-80 pubmed publisher
    ..have biochemically reconstituted two distinct populations of nucleosomes containing Saccharomyces cerevisiae CenH3 (Cse4)...
  4. Westermann S, Cheeseman I, Anderson S, Yates J, Drubin D, Barnes G. Architecture of the budding yeast kinetochore reveals a conserved molecular core. J Cell Biol. 2003;163:215-22 pubmed
    ..We propose that a molecular core consisting of CENP-A, -C, -H, and Ndc80/HEC has been conserved from yeast to humans to link centromeres to spindle microtubules. ..
  5. Huang C, Chang K, Cui H, Jayaram M. Histone H3-variant Cse4-induced positive DNA supercoiling in the yeast plasmid has implications for a plasmid origin of a chromosome centromere. Proc Natl Acad Sci U S A. 2011;108:13671-6 pubmed publisher
    ..The plasmid's partitioning system poaches host factors, including the centromere-specific histone H3-variant Cse4 and the cohesin complex, enabling replicated plasmid copies to segregate equally in a chromosome-coupled fashion...
  6. Gkikopoulos T, Singh V, Tsui K, Awad S, Renshaw M, Scholfield P, et al. The SWI/SNF complex acts to constrain distribution of the centromeric histone variant Cse4. EMBO J. 2011;30:1919-27 pubmed publisher
    ..the Snf2 subunit of the complex was found to cause partial redistribution of the centromeric histone variant Cse4 to sites on chromosome arms. Cultures of snf2Δ yeast were found to progress through mitosis slowly...
  7. Shivaraju M, Unruh J, Slaughter B, Mattingly M, Berman J, Gerton J. Cell-cycle-coupled structural oscillation of centromeric nucleosomes in yeast. Cell. 2012;150:304-16 pubmed publisher
    ..Centromeres are marked by a histone H3 variant. In budding yeast, the histone H3 variant Cse4 is present in a single centromeric nucleosome...
  8. Luconi L, Araki Y, Erlemann S, Schiebel E. The CENP-A chaperone Scm3 becomes enriched at kinetochores in anaphase independently of CENP-A incorporation. Cell Cycle. 2011;10:3369-78 pubmed publisher
    ..the canonical histones H2A, H2B and H4 and a centromere-specific histone H3 variant, known as CENP-A in humans and Cse4 in budding yeast...
  9. Meluh P, Yang P, Glowczewski L, Koshland D, Smith M. Cse4p is a component of the core centromere of Saccharomyces cerevisiae. Cell. 1998;94:607-13 pubmed
    ..Furthermore, by immunofluorescence microscopy, Cse4p is found in discrete foci consistent with that expected for centromeres. These results suggest the kinetochore is assembled on a specialized centromeric nucleosome containing Cse4p. ..
  10. Smith M, Yang P, Santisteban M, Boone P, Goldstein A, Megee P. A novel histone H4 mutant defective in nuclear division and mitotic chromosome transmission. Mol Cell Biol. 1996;16:1017-26 pubmed
    ..High-copy CSE4, encoding an H3 variant related to the mammalian CENP-A kinetochore antigen, was found to suppress the temperature ..
  11. Zhou Z, Feng H, Zhou B, Ghirlando R, Hu K, Zwolak A, et al. Structural basis for recognition of centromere histone variant CenH3 by the chaperone Scm3. Nature. 2011;472:234-7 pubmed publisher
    ..we have elucidated the structural basis for recognition of budding yeast (Saccharomyces cerevisiae) CenH3 (called Cse4) by Scm3...
  12. Chen Y, Baker R, Keith K, Harris K, Stoler S, Fitzgerald Hayes M. The N terminus of the centromere H3-like protein Cse4p performs an essential function distinct from that of the histone fold domain. Mol Cell Biol. 2000;20:7037-48 pubmed
    ..Genetic and biochemical evidence shows that Cse4p proteins interact with each other in vivo and that nonfunctional cse4 END and HFD mutant proteins can form functional mixed complexes...
  13. Shivaraju M, Camahort R, Mattingly M, Gerton J. Scm3 is a centromeric nucleosome assembly factor. J Biol Chem. 2011;286:12016-23 pubmed publisher
    The Cse4 nucleosome at each budding yeast centromere must be faithfully assembled each cell cycle to specify the site of kinetochore assembly and microtubule attachment for chromosome segregation...
  14. Akiyoshi B, Nelson C, Biggins S. The aurora B kinase promotes inner and outer kinetochore interactions in budding yeast. Genetics. 2013;194:785-9 pubmed publisher
    ..The Dsn1 component is crucial for kinetochore assembly and is phosphorylated by the Aurora B kinase. We found that Aurora B phosphorylation of Dsn1 promotes the interaction between outer and inner kinetochore proteins in budding yeast. ..
  15. Lochmann B, Ivanov D. Histone H3 localizes to the centromeric DNA in budding yeast. PLoS Genet. 2012;8:e1002739 pubmed publisher
    ..In budding yeast, as well as in other eukaryotes, the Cse4 histone variant (known in vertebrates as CENP-A) is believed to substitute for histone H3 at the centromeric ..
  16. Choy J, Acuña R, Au W, Basrai M. A role for histone H4K16 hypoacetylation in Saccharomyces cerevisiae kinetochore function. Genetics. 2011;189:11-21 pubmed publisher
    ..Inhibition of histone deacetylases (HDAC) using nicotinamide (NAM) caused lethality in cse4 and hhf1-20 kinetochore mutants and increased centromeric H4K16Ac...
  17. Mizuguchi G, Xiao H, Wisniewski J, Smith M, Wu C. Nonhistone Scm3 and histones CenH3-H4 assemble the core of centromere-specific nucleosomes. Cell. 2007;129:1153-64 pubmed
    The budding yeast histone H3 variant, Cse4, replaces conventional histone H3 in centromeric chromatin and, together with centromere-specific DNA-binding factors, directs assembly of the kinetochore, a multiprotein complex mediating ..
  18. Collins K, Castillo A, Tatsutani S, Biggins S. De novo kinetochore assembly requires the centromeric histone H3 variant. Mol Biol Cell. 2005;16:5649-60 pubmed
    ..We performed the first investigation of kinetochore assembly in the absence of the centromeric histone H3 variant Cse4 and found that all proteins tested depend on Cse4 to localize...
  19. Hsu J, Huang J, Meluh P, Laurent B. The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation. Mol Cell Biol. 2003;23:3202-15 pubmed
  20. Au W, Crisp M, DeLuca S, Rando O, Basrai M. Altered dosage and mislocalization of histone H3 and Cse4p lead to chromosome loss in Saccharomyces cerevisiae. Genetics. 2008;179:263-75 pubmed publisher
    ..Overexpression of the stable Cse4p mutant, cse4(K16R), resulted in its mislocalization, increased association with chromatin, and a high rate of chromosome loss, ..
  21. Huang C, Hajra S, Ghosh S, Jayaram M. Cse4 (CenH3) association with the Saccharomyces cerevisiae plasmid partitioning locus in its native and chromosomally integrated states: implications in centromere evolution. Mol Cell Biol. 2011;31:1030-40 pubmed publisher
    The histone H3 variant Cse4 specifies centromere identity in Saccharomyces cerevisiae by its incorporation into a special nucleosome positioned at CEN DNA and promotes the assembly of the kinetochore complex, which is required for ..
  22. Aravamudhan P, Felzer Kim I, Joglekar A. The budding yeast point centromere associates with two Cse4 molecules during mitosis. Curr Biol. 2013;23:770-4 pubmed publisher
    ..Using novel live-cell fluorescence microscopy assays, we demonstrate that the budding yeast centromere recruits two Cse4 (ScCENP-A) molecules...
  23. Sharp J, Franco A, Osley M, Kaufman P. Chromatin assembly factor I and Hir proteins contribute to building functional kinetochores in S. cerevisiae. Genes Dev. 2002;16:85-100 pubmed
    ..Finally, CAF-I subunits and Hir1 are enriched at centromeres, indicating that these proteins make a direct contribution to centromeric chromatin structures. ..
  24. Baker R, Harris K, Zhang K. Mutations synthetically lethal with cep1 target S. cerevisiae kinetochore components. Genetics. 1998;149:73-85 pubmed
    ..Three of these four CSL genes were subsequently found to be known or imputed kinetochore genes: CEP3, NDC10, and CSE4. The fourth, CSL4, corresponded to ORF YNL232w on chromosome XIV, and was found to be essential...
  25. Camahort R, Li B, Florens L, Swanson S, Washburn M, Gerton J. Scm3 is essential to recruit the histone h3 variant cse4 to centromeres and to maintain a functional kinetochore. Mol Cell. 2007;26:853-65 pubmed
    ..Budding-yeast Cse4 is an essential evolutionarily conserved histone H3 variant recruited to the centromere by an unknown mechanism...
  26. Samel A, Cuomo A, Bonaldi T, Ehrenhofer Murray A. Methylation of CenH3 arginine 37 regulates kinetochore integrity and chromosome segregation. Proc Natl Acad Sci U S A. 2012;109:9029-34 pubmed publisher
    ..Here we show that CENP-A from Saccharomyces cerevisiae, termed Cse4, is methylated on arginine 37 (R37) and that this methylation regulates the recruitment of kinetochore components ..
  27. Pinsky B, Tatsutani S, Collins K, Biggins S. An Mtw1 complex promotes kinetochore biorientation that is monitored by the Ipl1/Aurora protein kinase. Dev Cell. 2003;5:735-45 pubmed
    ..dosage suppressors and identified Dsn1, a kinetochore protein that immunoprecipitates with the Mif2/CENP-C and Cse4/CENP-A proteins, as well as the Mtw1, Nnf1, and Nsl1 kinetochore proteins...
  28. Stoler S, Rogers K, Weitze S, Morey L, Fitzgerald Hayes M, Baker R. Scm3, an essential Saccharomyces cerevisiae centromere protein required for G2/M progression and Cse4 localization. Proc Natl Acad Sci U S A. 2007;104:10571-6 pubmed
    ..to identify factors involved in CenH3 deposition, we screened for dosage suppressors of a temperature-sensitive cse4 allele in Saccharomyces cerevisiae (Cse4 is the S. cerevisiae CenH3)...
  29. Mythreye K, Bloom K. Differential kinetochore protein requirements for establishment versus propagation of centromere activity in Saccharomyces cerevisiae. J Cell Biol. 2003;160:833-43 pubmed
    ..Furthermore, this study identifies Chl4p in the initiation and specification of a heritable chromatin state. ..
  30. Measday V, Hailey D, Pot I, Givan S, Hyland K, Cagney G, et al. Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore. Genes Dev. 2002;16:101-13 pubmed
    ..In contrast, Ctf3p and Ctf19p fail to bind properly to the centromere in a cse4-1 mutant strain...
  31. Pinto I, Winston F. Histone H2A is required for normal centromere function in Saccharomyces cerevisiae. EMBO J. 2000;19:1598-612 pubmed
    ..Taken together, these results strongly suggest that histone H2A is required for proper centromere-kinetochore function during chromosome segregation. ..
  32. Stoler S, Keith K, Curnick K, Fitzgerald Hayes M. A mutation in CSE4, an essential gene encoding a novel chromatin-associated protein in yeast, causes chromosome nondisjunction and cell cycle arrest at mitosis. Genes Dev. 1995;9:573-86 pubmed
    ..In this study, a Saccharomyces cerevisiae chromosome mis-segregation mutant, cse4-1, has been isolated and shown to increase the nondisjunction frequency of a chromosome bearing a mutant centromere ..
  33. Durand Dubief M, Will W, Petrini E, Theodorou D, Harris R, Crawford M, et al. SWI/SNF-like chromatin remodeling factor Fun30 supports point centromere function in S. cerevisiae. PLoS Genet. 2012;8:e1002974 pubmed publisher
    ..FUN30 genetically interacts with CSE4, coding for the centromere-specific variant of histone H3, and counteracts the detrimental effect of transcription ..
  34. Snead J, Sullivan M, Lowery D, Cohen M, Zhang C, Randle D, et al. A coupled chemical-genetic and bioinformatic approach to Polo-like kinase pathway exploration. Chem Biol. 2007;14:1261-72 pubmed
    ..Finally, an examination of Cdc5 binding by SPB-localized proteins expanded our knowledge of Cdc5 function at the SPB. ..
  35. Mishra P, Guo J, Dittman L, Haase J, Yeh E, Bloom K, et al. Pat1 protects centromere-specific histone H3 variant Cse4 from Psh1-mediated ubiquitination. Mol Biol Cell. 2015;26:2067-79 pubmed publisher
    Evolutionarily conserved histone H3 variant Cse4 and its homologues are essential components of specialized centromere (CEN)-specific nucleosomes and serve as an epigenetic mark for CEN identity and propagation...
  36. Deyter G, Hildebrand E, Barber A, Biggins S. Histone H4 Facilitates the Proteolysis of the Budding Yeast CENP-ACse4 Centromeric Histone Variant. Genetics. 2017;205:113-124 pubmed publisher
    ..that facilitate proteolysis, we performed a genetic screen to identify histone H4 residues that regulate CENP-ACse4 degradation...
  37. Daniel J. Direct in vivo access to potential gene targets of the RPD3 histone deactylase using fitness-based interferential genetics. Yeast. 2007;24:575-87 pubmed
    ..Moreover, other selected genes are linked to cell-cycle control (CSE4, AMN1, VAC17 and GRR1)...
  38. Deyter G, Biggins S. The FACT complex interacts with the E3 ubiquitin ligase Psh1 to prevent ectopic localization of CENP-A. Genes Dev. 2014;28:1815-26 pubmed publisher
    ..complex FACT (facilitates chromatin transcription/transactions) in preventing budding yeast CENP-A(Cse4) mislocalization to euchromatin by mediating its proteolysis...
  39. Hajra S, Ghosh S, Jayaram M. The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-microm circle partitioning locus and promotes equal plasmid segregation. J Cell Biol. 2006;174:779-90 pubmed
    ..It has been regarded as the exquisite hallmark of centromeric chromatin. We demonstrate that Cse4 resides at the partitioning locus STB of the 2-microm plasmid...
  40. Hewawasam G, Mattingly M, Venkatesh S, Zhang Y, Florens L, Workman J, et al. Phosphorylation by casein kinase 2 facilitates Psh1 protein-assisted degradation of Cse4 protein. J Biol Chem. 2014;289:29297-309 pubmed publisher
    b>Cse4 is the centromeric histone H3 variant in budding yeast. Psh1 is an E3 ubiquitin ligase that controls Cse4 levels through proteolysis...
  41. Hornung P, Troć P, Malvezzi F, Maier M, Demianova Z, Zimniak T, et al. A cooperative mechanism drives budding yeast kinetochore assembly downstream of CENP-A. J Cell Biol. 2014;206:509-24 pubmed publisher
    ..This two-step mechanism may protect against inappropriate kinetochore assembly similar to rate-limiting nucleation steps used by cytoskeletal polymers. ..
  42. Wisniewski J, Hajj B, Chen J, Mizuguchi G, Xiao H, Wei D, et al. Imaging the fate of histone Cse4 reveals de novo replacement in S phase and subsequent stable residence at centromeres. elife. 2014;3:e02203 pubmed publisher
    The budding yeast centromere contains Cse4, a specialized histone H3 variant...
  43. Ho K, Tsuchiya D, Oliger A, Lacefield S. Localization and function of budding yeast CENP-A depends upon kinetochore protein interactions and is independent of canonical centromere sequence. Cell Rep. 2014;9:2027-33 pubmed publisher
    ..The localization of budding yeast CENP-A, Cse4, requires the centromere DNA binding components, which are not conserved in multicellular eukaryotes...
  44. Kanta H, Laprade L, Almutairi A, Pinto I. Suppressor analysis of a histone defect identifies a new function for the hda1 complex in chromosome segregation. Genetics. 2006;173:435-50 pubmed
    ..Moreover, these suppressors provide the basis for future studies regarding histone function in chromosome segregation. ..
  45. Ohkuni K, Abdulle R, Tong A, Boone C, Kitagawa K. Ybp2 associates with the central kinetochore of Saccharomyces cerevisiae and mediates proper mitotic progression. PLoS ONE. 2008;3:e1617 pubmed publisher
    ..and 3 components of the Ndc80 complex (Ndc80, Nuf2, and Spc25 but not Spc24) in the central kinetochore and with Cse4 (the centromeric histone and CENP-A homolog)...
  46. Mishra P, Thapa K, Chen P, Wang S, Hazbun T, Basrai M. Budding yeast CENP-ACse4 interacts with the N-terminus of Sgo1 and regulates its association with centromeric chromatin. Cell Cycle. 2018;17:11-23 pubmed publisher
    ..Given that histone H3 is absent from CEN and is replaced by a histone H3 variant CENP-ACse4, we investigated if CENP-ACse4 interacts with Sgo1 and promotes its association with the CEN ..
  47. White W, Johnson D. Characterization of synthetic-lethal mutants reveals a role for the Saccharomyces cerevisiae guanine-nucleotide exchange factor Cdc24p in vacuole function and Na+ tolerance. Genetics. 1997;147:43-55 pubmed
    ..Our results indicated that Cdc24p was required in one V-ATPase mutant and another mutant affecting vacuole morphology, and also implicated Cdc24p in Na+ tolerance. ..
  48. Miller M, Asbury C, Biggins S. A TOG Protein Confers Tension Sensitivity to Kinetochore-Microtubule Attachments. Cell. 2016;165:1428-1439 pubmed publisher
  49. Anderson M, Haase J, Yeh E, Bloom K. Function and assembly of DNA looping, clustering, and microtubule attachment complexes within a eukaryotic kinetochore. Mol Biol Cell. 2009;20:4131-9 pubmed publisher
    ..The linkage of microtubule-binding to centromere DNA-looping complexes positions the pericentric chromatin loops and stabilizes the dynamic properties of individual kinetochore complexes in mitosis. ..
  50. Au W, Dawson A, Rawson D, Taylor S, Baker R, Basrai M. A novel role of the N terminus of budding yeast histone H3 variant Cse4 in ubiquitin-mediated proteolysis. Genetics. 2013;194:513-8 pubmed publisher
    ..Interaction of Psh1, an E3 ligase, with the C terminus of Cse4 has been shown to contribute to its proteolysis...
  51. Haase J, Stephens A, Verdaasdonk J, Yeh E, Bloom K. Bub1 kinase and Sgo1 modulate pericentric chromatin in response to altered microtubule dynamics. Curr Biol. 2012;22:471-81 pubmed publisher
    ..The geometric alteration of all 16 kinetochores and pericentric chromatin reflect global changes in the pericentromeric region and provide mechanisms for mechanically amplifying damage at a single kinetochore microtubule. ..
  52. Sarangapani K, Akiyoshi B, Duggan N, Biggins S, Asbury C. Phosphoregulation promotes release of kinetochores from dynamic microtubules via multiple mechanisms. Proc Natl Acad Sci U S A. 2013;110:7282-7 pubmed publisher
    ..Taken together, our results suggest that, in vivo, Aurora B releases kinetochores via at least two mechanisms: by weakening the kinetochore-microtubule interface and also by destabilizing the kinetochore-attached microtubule tip...
  53. Henikoff S, Ramachandran S, Krassovsky K, Bryson T, Codomo C, Brogaard K, et al. The budding yeast Centromere DNA Element II wraps a stable Cse4 hemisome in either orientation in vivo. elife. 2014;3:e01861 pubmed publisher
    In budding yeast, a single cenH3 (Cse4) nucleosome occupies the ?120-bp functional centromere, however conflicting structural models for the particle have been proposed...
  54. Ohkuni K, Takahashi Y, Fulp A, Lawrimore J, Au W, Pasupala N, et al. SUMO-Targeted Ubiquitin Ligase (STUbL) Slx5 regulates proteolysis of centromeric histone H3 variant Cse4 and prevents its mislocalization to euchromatin. Mol Biol Cell. 2016;: pubmed
    Centromeric histone H3, CENP-ACse4, is essential for faithful chromosome segregation. Stringent regulation of cellular levels of CENP-ACse4 restricts its localization to centromeres...
  55. Cheng H, Bao X, Rao H. The F-box Protein Rcy1 Is Involved in the Degradation of Histone H3 Variant Cse4 and Genome Maintenance. J Biol Chem. 2016;291:10372-7 pubmed publisher
    b>Cse4, a histone H3-like centromeric protein, plays critical functions in chromosome segregation. Cse4 level is tightly regulated, but the underlying mechanism remains poorly understood...
  56. Boeckmann L, Takahashi Y, Au W, Mishra P, Choy J, Dawson A, et al. Phosphorylation of centromeric histone H3 variant regulates chromosome segregation in Saccharomyces cerevisiae. Mol Biol Cell. 2013;24:2034-44 pubmed publisher
    ..We identify posttranslational modifications of Saccharomyces cerevisiae CenH3, Cse4. Functional characterization of cse4 phosphorylation mutants shows growth and chromosome segregation defects when ..
  57. Canzonetta C, Vernarecci S, Iuliani M, Marracino C, Belloni C, Ballario P, et al. SAGA DUB-Ubp8 Deubiquitylates Centromeric Histone Variant Cse4. G3 (Bethesda). 2015;6:287-98 pubmed publisher
    ..site to the mitotic spindle, marked by specialized nucleosomes containing a specific histone variant, CEN-H3/Cse4, in yeast...
  58. Peiró Chova L, Estruch F. Specific defects in different transcription complexes compensate for the requirement of the negative cofactor 2 repressor in Saccharomyces cerevisiae. Genetics. 2007;176:125-38 pubmed