Gene Symbol: CRH1
Species: Saccharomyces cerevisiae S288c
Rodríguez Peña J, Cid V, Arroyo J, Nombela C. A novel family of cell wall-related proteins regulated differently during the yeast life cycle. Mol Cell Biol. 2000;20:3245-55 pubmed
..These genes were renamed CRH1 and CRH2, respectively, for Congo red hypersensitive...
Blanco N, Sanz A, RodrÃguez PeÃ±a J, Nombela C, FarkaÅ¡ V, Hurtado Guerrero R, et al
. Structural and functional analysis of yeast Crh1 and Crh2 transglycosylases. FEBS J. 2015;282:715-31 pubmed publisher
Covalent cross-links between chitin and glucan at the yeast cell wall are created by the transglycosylase activity of redundant proteins Crh1 and Crh2, with cleavage of Î²-1,4 linkages of the chitin backbone and transfer of the ..
KovÃ¡ÄovÃ¡ K, FarkaÅ¡ V. Two Variants of a High-Throughput Fluorescent Microplate Assay of Polysaccharide Endotransglycosylases. Appl Biochem Biotechnol. 2016;178:1652-65 pubmed publisher
Yin Q, De Groot P, Dekker H, De Jong L, Klis F, de Koster C. Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages. J Biol Chem. 2005;280:20894-901 pubmed
..Therefore, it will probably be equally effective in many other fungi. ..
Cabib E, Blanco N, Grau C, Rodriguez Peña J, Arroyo J. Crh1p and Crh2p are required for the cross-linking of chitin to beta(1-6)glucan in the Saccharomyces cerevisiae cell wall. Mol Microbiol. 2007;63:921-35 pubmed
..study of cell wall cross-links, we have found that chitin linked to beta(1-6)glucan is diminished in mutants of the CRH1 or the CRH2/UTR2 gene and completely absent in a double mutant...
Cabib E, Farkas V, Kosík O, Blanco N, Arroyo J, McPhie P. Assembly of the yeast cell wall. Crh1p and Crh2p act as transglycosylases in vivo and in vitro. J Biol Chem. 2008;283:29859-72 pubmed publisher
..The importance of transglycosylation for cell wall assembly is thus firmly established. ..
Cabib E. Two novel techniques for determination of polysaccharide cross-links show that Crh1p and Crh2p attach chitin to both beta(1-6)- and beta(1-3)glucan in the Saccharomyces cerevisiae cell wall. Eukaryot Cell. 2009;8:1626-36 pubmed publisher
..Therefore, Crh1p and Crh2p catalyze the transfer of chitin to both beta(1-3)- and beta(1-6)glucan, and the biosynthetic mechanism for all chitin cross-links in the cell wall has been established. ..
Blanco N, Reidy M, Arroyo J, Cabib E. Crosslinks in the cell wall of budding yeast control morphogenesis at the mother-bud neck. J Cell Sci. 2012;125:5781-9 pubmed publisher
..When in a cla4? background, crh1? crh2? mutants, in which the chitin ring is not connected to ?(1-3)glucan, grew very slowly and showed wide and ..
Mazán M, Blanco N, Kováčová K, Firáková Z, Rehulka P, Farkas V, et al
. A novel fluorescence assay and catalytic properties of Crh1 and Crh2 yeast cell wall transglycosylases. Biochem J. 2013;455:307-18 pubmed publisher
..In the present study, the proteins encoded by CRH1 and CRH2 were heterologously expressed in Pichia pastoris and a sensitive fluorescence in vitro soluble assay was ..