Gene Symbol: CRD1
Description: cardiolipin synthase
Alias: CLS1, cardiolipin synthase
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Jiang F, Ryan M, Schlame M, Zhao M, Gu Z, Klingenberg M, et al. Absence of cardiolipin in the crd1 null mutant results in decreased mitochondrial membrane potential and reduced mitochondrial function. J Biol Chem. 2000;275:22387-94 pubmed
    ..Saccharomyces cerevisiae cells containing a disruption of CRD1, the structural gene encoding CL synthase, have no CL in mitochondrial membranes...
  2. Chen S, Tarsio M, Kane P, Greenberg M. Cardiolipin mediates cross-talk between mitochondria and the vacuole. Mol Biol Cell. 2008;19:5047-58 pubmed publisher
    ..2000; Zhong et al., 2004). Previous studies have shown that yeast mutant cells lacking CL due to a disruption in CRD1, the structural gene encoding CL synthase, exhibit defective colony formation at elevated temperature even on ..
  3. Yadav P, Rajasekharan R. Misregulation of a DDHD Domain-containing Lipase Causes Mitochondrial Dysfunction in Yeast. J Biol Chem. 2016;291:18562-81 pubmed publisher
    ..Therefore, the DDL1-defective strain could be a good model system for understanding hereditary spastic paraplegia. ..
  4. Gohil V, Thompson M, Greenberg M. Synthetic lethal interaction of the mitochondrial phosphatidylethanolamine and cardiolipin biosynthetic pathways in Saccharomyces cerevisiae. J Biol Chem. 2005;280:35410-6 pubmed
    ..These results suggest that mitochondrial biosynthesis of PE is essential for the viability of yeast mutants lacking CL. ..
  5. Sauerwald J, Jores T, Eisenberg Bord M, Chuartzman S, Schuldiner M, Rapaport D. Genome-Wide Screens in Saccharomyces cerevisiae Highlight a Role for Cardiolipin in Biogenesis of Mitochondrial Outer Membrane Multispan Proteins. Mol Cell Biol. 2015;35:3200-11 pubmed publisher
    ..Cardiolipin (CL) synthase (Crd1) appeared as a candidate in both screens...
  6. Joshi A, Thompson M, Fei N, Huttemann M, Greenberg M. Cardiolipin and mitochondrial phosphatidylethanolamine have overlapping functions in mitochondrial fusion in Saccharomyces cerevisiae. J Biol Chem. 2012;287:17589-97 pubmed publisher
    ..To test this hypothesis, we constructed a conditional mutant crd1?psd1? containing null alleles of CRD1 (CL synthase) and PSD1 (mitochondrial phosphatidylserine decarboxylase), in ..
  7. Chang S, Heacock P, Mileykovskaya E, Voelker D, Dowhan W. Isolation and characterization of the gene (CLS1) encoding cardiolipin synthase in Saccharomyces cerevisiae. J Biol Chem. 1998;273:14933-41 pubmed
    ..using homology searches for genes potentially encoding phospholipid biosynthetic enzymes, we have cloned the gene (CLS1) encoding CL synthase in Saccharomyces cerevisiae...
  8. Watanabe Y, Tamura Y, Kawano S, Endo T. Structural and mechanistic insights into phospholipid transfer by Ups1-Mdm35 in mitochondria. Nat Commun. 2015;6:7922 pubmed publisher
    ..These results provide a structural basis for understanding the mechanism of PA transfer between mitochondrial membranes. ..
  9. Luévano Martínez L, Forni M, Dos Santos V, Souza Pinto N, Kowaltowski A. Cardiolipin is a key determinant for mtDNA stability and segregation during mitochondrial stress. Biochim Biophys Acta. 2015;1847:587-98 pubmed publisher
    ..Conversely, this effect is cardiolipin-dependent since a cardiolipin synthase-null mutant strain is unable to adapt to thermal stress as observed by a 60% increase of cells lacking ..

More Information


  1. Chen S, Liu D, Finley R, Greenberg M. Loss of mitochondrial DNA in the yeast cardiolipin synthase crd1 mutant leads to up-regulation of the protein kinase Swe1p that regulates the G2/M transition. J Biol Chem. 2010;285:10397-407 pubmed publisher
    ..These results suggest that anionic phospholipids are required for processes that are essential for normal cell division in rho degrees cells. ..
  2. Gebert N, Joshi A, Kutik S, Becker T, McKenzie M, Guan X, et al. Mitochondrial cardiolipin involved in outer-membrane protein biogenesis: implications for Barth syndrome. Curr Biol. 2009;19:2133-9 pubmed publisher
    ..Our findings reveal a new role for cardiolipin in protein sorting at the mitochondrial outer membrane and bear implications for the pathogenesis of Barth syndrome. ..
  3. Kornmann B, Currie E, Collins S, Schuldiner M, Nunnari J, Weissman J, et al. An ER-mitochondria tethering complex revealed by a synthetic biology screen. Science. 2009;325:477-81 pubmed publisher
    ..The tethering complex localized to discrete foci, suggesting that discrete sites of close apposition between ER and mitochondria facilitate interorganelle calcium and phospholipid exchange. ..
  4. Chen Y, Taylor E, Dephoure N, Heo J, Tonhato A, Papandreou I, et al. Identification of a protein mediating respiratory supercomplex stability. Cell Metab. 2012;15:348-60 pubmed publisher
    ..We suggest that Rcf1 is a member of an evolutionarily conserved protein family that acts to promote respiratory supercomplex assembly and activity. ..
  5. Rijken P, Houtkooper R, Akbari H, Brouwers J, Koorengevel M, de Kruijff B, et al. Cardiolipin molecular species with shorter acyl chains accumulate in Saccharomyces cerevisiae mutants lacking the acyl coenzyme A-binding protein Acb1p: new insights into acyl chain remodeling of cardiolipin. J Biol Chem. 2009;284:27609-19 pubmed publisher
    ..Experiments in which the substrate specificity of the yeast cardiolipin synthase Crd1p and the acyl chain composition of individual short CL species were investigated, indicated that both ..
  6. Schlame M, Zhao M, Rua D, Haldar D, Greenberg M. Kinetic analysis of cardiolipin synthase: a membrane enzyme with two glycerophospholipid substrates. Lipids. 1995;30:633-40 pubmed
    Mitochondrial cardiolipin synthase catalyzes the transfer of a phosphatidyl moiety from phosphatidyl-CMP (PtdCMP) to phosphatidylglycerol (PtdGro) in the presence of specific divalent cations...
  7. Gat Viks I, Meller R, Kupiec M, Shamir R. Understanding gene sequence variation in the context of transcription regulation in yeast. PLoS Genet. 2010;6:e1000800 pubmed publisher
    ..Overall, our findings demonstrate that this approach provides a useful framework for the systematic mapping of quantitative trait loci and their role in gene expression variation. ..
  8. Patil V, Fox J, Gohil V, Winge D, Greenberg M. Loss of cardiolipin leads to perturbation of mitochondrial and cellular iron homeostasis. J Biol Chem. 2013;288:1696-705 pubmed publisher
    ..the cellular response to the loss of CL, we carried out genome-wide expression profiling of the yeast CL mutant crd1?...
  9. Joshi A, Fei N, Greenberg M. Get1p and Get2p are required for maintenance of mitochondrial morphology and normal cardiolipin levels. FEMS Yeast Res. 2016;16: pubmed publisher
    ..The genetic interactions observed in this study identify novel physiological modifiers that are required for maintenance of CL levels and mitochondrial morphology. ..
  10. Shen Z, Li Y, Gasparski A, Abeliovich H, Greenberg M. Cardiolipin Regulates Mitophagy through the Protein Kinase C Pathway. J Biol Chem. 2017;292:2916-2923 pubmed publisher
    ..genes ATG8, ATG18, and ATG32 synthetically interact with CL synthase mutant crd1Δ...