COX5A

Summary

Gene Symbol: COX5A
Description: cytochrome c oxidase subunit Va
Alias: cytochrome c oxidase subunit Va
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Waterland R, Basu A, Chance B, Poyton R. The isoforms of yeast cytochrome c oxidase subunit V alter the in vivo kinetic properties of the holoenzyme. J Biol Chem. 1991;266:4180-6 pubmed
    ..One of the nuclear-coded subunits of yeast cytochrome c oxidase is specified by a gene family composed of two genes, COX5a and COX5b...
  2. Horan S, Bourges I, Taanman J, Meunier B. Analysis of COX2 mutants reveals cytochrome oxidase subassemblies in yeast. Biochem J. 2005;390:703-8 pubmed
    ..The identification of these novel cytochrome oxidase subcomplexes should encourage the reexamination of other yeast mutants. ..
  3. Fontanesi F, Jin C, Tzagoloff A, Barrientos A. Transcriptional activators HAP/NF-Y rescue a cytochrome c oxidase defect in yeast and human cells. Hum Mol Genet. 2008;17:775-88 pubmed
    ..Analogously, overexpression of the Hap complex human homologue NF-YA/B/C transcription complex in SURF1-deficient fibroblasts from an LS patient efficiently rescues their COX deficiency. ..
  4. Mick D, Wagner K, van der Laan M, Frazier A, Perschil I, Pawlas M, et al. Shy1 couples Cox1 translational regulation to cytochrome c oxidase assembly. EMBO J. 2007;26:4347-58 pubmed
    ..We suggest that Shy1 links Cox1 translational regulation to complex IV assembly and supercomplex formation. ..
  5. Fontanesi F, Clemente P, Barrientos A. Cox25 teams up with Mss51, Ssc1, and Cox14 to regulate mitochondrial cytochrome c oxidase subunit 1 expression and assembly in Saccharomyces cerevisiae. J Biol Chem. 2011;286:555-66 pubmed publisher
    ..These results suggest that once Ssc1-Mss51 are released from the Cox1 stabilization complex, Cox25 continues to interact with Cox14 and Cox1 to facilitate the formation of multisubunit COX assembly intermediates. ..
  6. Chen Y, Taylor E, Dephoure N, Heo J, Tonhato A, Papandreou I, et al. Identification of a protein mediating respiratory supercomplex stability. Cell Metab. 2012;15:348-60 pubmed publisher
    ..We suggest that Rcf1 is a member of an evolutionarily conserved protein family that acts to promote respiratory supercomplex assembly and activity. ..
  7. Dodia R, Meunier B, Kay C, Rich P. Comparisons of subunit 5A and 5B isoenzymes of yeast cytochrome c oxidase. Biochem J. 2014;464:335-42 pubmed publisher
    ..5A is expressed under normoxic conditions, whereas 5B is expressed at very low oxygen tensions. As a consequence, COX5A-deleted strains (Δcox5A) have no or only low levels of CcO under normoxic conditions rendering them ..
  8. Taanman J, Capaldi R. Purification of yeast cytochrome c oxidase with a subunit composition resembling the mammalian enzyme. J Biol Chem. 1992;267:22481-5 pubmed
    ..The enzyme isolated in lauryl maltoside contains these same nine different polypeptides and three others, including homologues of subunits VIa and VIb of the mammalian enzyme. ..
  9. Meier S, Neupert W, Herrmann J. Conserved N-terminal negative charges in the Tim17 subunit of the TIM23 translocase play a critical role in the import of preproteins into mitochondria. J Biol Chem. 2005;280:7777-85 pubmed
    ..On the basis of these observations we propose that charged residues in Tim17 are critical for the preprotein-induced gating of the TIM23 translocase. ..

More Information

Publications16

  1. Pierrel F, Khalimonchuk O, Cobine P, Bestwick M, Winge D. Coa2 is an assembly factor for yeast cytochrome c oxidase biogenesis that facilitates the maturation of Cox1. Mol Cell Biol. 2008;28:4927-39 pubmed publisher
    ..Overexpression of Cox10 or Cox5a and Cox6 or attenuation of the proteolytic activity of the m-AAA protease partially restores respiration in ..
  2. Stuart R. Chapter 11 Supercomplex organization of the yeast respiratory chain complexes and the ADP/ATP carrier proteins. Methods Enzymol. 2009;456:191-208 pubmed publisher
    ..The method used to analyze the cytochrome bc(1)-COX-AAC supercomplex and to affinity purify the Aac2 isoform and its associating proteins from S. cerevisiae mitochondria will be outlined in this chapter. ..
  3. Levchenko M, Wuttke J, Römpler K, Schmidt B, Neifer K, Juris L, et al. Cox26 is a novel stoichiometric subunit of the yeast cytochrome c oxidase. Biochim Biophys Acta. 2016;1863:1624-32 pubmed publisher
    ..Our analyses reveal Cox26 as a novel stoichiometric structural subunit of the cytochrome c oxidase. A loss of Cox26 affects cytochrome c oxidase activity and respirasome organization. ..
  4. Castello P, Woo D, Ball K, Wojcik J, Liu L, Poyton R. Oxygen-regulated isoforms of cytochrome c oxidase have differential effects on its nitric oxide production and on hypoxic signaling. Proc Natl Acad Sci U S A. 2008;105:8203-8 pubmed publisher
    ..studies have shown that these isoforms have differential effects on oxygen reduction by Cco, and that their genes (COX5a and COX5b, respectively) are inversely regulated by oxygen...
  5. Mick D, Vukotic M, Piechura H, Meyer H, Warscheid B, Deckers M, et al. Coa3 and Cox14 are essential for negative feedback regulation of COX1 translation in mitochondria. J Cell Biol. 2010;191:141-54 pubmed publisher
    ..Our data indicate that Coa1 binding to sequestered Mss51 in complex with Cox14, Coa3, and Cox1 is essential for full inactivation. ..
  6. Strogolova V, Furness A, Robb McGrath M, Garlich J, Stuart R. Rcf1 and Rcf2, members of the hypoxia-induced gene 1 protein family, are critical components of the mitochondrial cytochrome bc1-cytochrome c oxidase supercomplex. Mol Cell Biol. 2012;32:1363-73 pubmed publisher
    ..Based on our findings, we propose a model where the Hig1 proteins regulate the COX enzyme activity through Cox3 and associated Cox12 protein, in a manner that may be influenced by the neighboring AAC proteins. ..
  7. Bareth B, Nikolov M, Lorenzi I, Hildenbeutel M, Mick D, Helbig C, et al. Oms1 associates with cytochrome c oxidase assembly intermediates to stabilize newly synthesized Cox1. Mol Biol Cell. 2016;27:1570-80 pubmed publisher
    ..Our analyses show that Oms1 participates in cytochrome c oxidase assembly by stabilizing newly synthesized Cox1. ..