Gene Symbol: COB
Description: cytochrome b
Alias: COB1, CYTB, cytochrome b
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Hunte C, Koepke J, Lange C, Rossmanith T, Michel H. Structure at 2.3 A resolution of the cytochrome bc(1) complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment. Structure. 2000;8:669-84 pubmed
    ..Each 'monomer' of the refined model includes 2178 amino acid residues of subunits COR1, QCR2, COB, CYT1, RIP1, QCR6, QCR7, QCR8 and QCR9 of the cytochrome bc(1) complex and of the polypeptides V(H) and V(L) of the ..
  2. Atkinson A, Smith P, Fox J, Cui T, Khalimonchuk O, Winge D. The LYR protein Mzm1 functions in the insertion of the Rieske Fe/S protein in yeast mitochondria. Mol Cell Biol. 2011;31:3988-96 pubmed publisher
  3. Cruciat C, Hell K, FOLSCH H, Neupert W, Stuart R. Bcs1p, an AAA-family member, is a chaperone for the assembly of the cytochrome bc(1) complex. EMBO J. 1999;18:5226-33 pubmed
    ..We propose that Bcs1p acts as an ATP-dependent chaperone, maintaining the precomplex in a competent state for the subsequent assembly of the Rieske FeS and Qcr10p proteins. ..
  4. Hell K, Neupert W, Stuart R. Oxa1p acts as a general membrane insertion machinery for proteins encoded by mitochondrial DNA. EMBO J. 2001;20:1281-8 pubmed
    ..Taken together, we conclude that the Oxa1 complex represents a general membrane protein insertion machinery in the inner membrane of mitochondria. ..
  5. Gruschke S, Römpler K, Hildenbeutel M, Kehrein K, Kühl I, Bonnefoy N, et al. The Cbp3-Cbp6 complex coordinates cytochrome b synthesis with bc(1) complex assembly in yeast mitochondria. J Cell Biol. 2012;199:137-50 pubmed publisher
    ..example, the bc(1) complex consists of nine nuclear encoded subunits and the mitochondrially encoded subunit cytochrome b. We recently showed that the Cbp3-Cbp6 complex has a dual function for biogenesis of cytochrome b: it is both ..
  6. Hildenbeutel M, Hegg E, Stephan K, Gruschke S, Meunier B, Ott M. Assembly factors monitor sequential hemylation of cytochrome b to regulate mitochondrial translation. J Cell Biol. 2014;205:511-24 pubmed publisher
    ..How these redox cofactors are assembled into the complexes is not known. Cytochrome b, a central catalytic subunit of complex III, contains two heme bs...
  7. Moreno J, Buie K, Price R, Piva M. Ccm1p/Ygr150cp, a pentatricopeptide repeat protein, is essential to remove the fourth intron of both COB and COX1 pre-mRNAs in Saccharomyces cerevisiae. Curr Genet. 2009;55:475-84 pubmed publisher
    ..RNA hybridization studies showed that Deltaygr150c meiotic segregants produced COB and COX1 transcripts but failed to process them into the mature forms...
  8. Bonjardim C, Nobrega F. Revision of the nucleotide sequence at the last intron of the mitochondrial apocytochrome b gene in Saccharomyces cerevisiae. Braz J Med Biol Res. 1984;17:17-20 pubmed
    ..The corrected sequence has four additional nucleotides, one Hae III and two Hpa II sites. Previously undetected typographical errors were also found in the A + T-rich sequence. ..
  9. Lazowska J, Jacq C, Slonimski P. Sequence of introns and flanking exons in wild-type and box3 mutants of cytochrome b reveals an interlaced splicing protein coded by an intron. Cell. 1980;22:333-48 pubmed
    ..of the wild type and mutated introns as well as their flanking exons in the yeast mitochondrial gene specifying cytochrome b. The second intron (box3) encodes a trans-acting protein "mRNA maturase" responsible for splicing and ..

More Information


  1. Lazowska J, Jacq C, Slonimski P. Splice points of the third intron in the yeast mitochondrial cytochrome b gene. Cell. 1981;27:12-4 pubmed
    We report the nucleotide sequences at the splicing junctions of intron 13 of the cytochrome b (box) "long" gene of the mitochondrion of Saccharomyces cerevisiae and compare them with the homologous sequences in Aspergillus ..
  2. Perez Martinez X, Butler C, Shingu Vazquez M, Fox T. Dual functions of Mss51 couple synthesis of Cox1 to assembly of cytochrome c oxidase in Saccharomyces cerevisiae mitochondria. Mol Biol Cell. 2009;20:4371-80 pubmed publisher
    ..Regulation of COX1 mRNA translation by Mss51 seems to be an example of a homeostatic mechanism in which a positive effector of gene expression interacts with the product it regulates in a posttranslational assembly process. ..
  3. Hill J, McGraw P, Tzagoloff A. A mutation in yeast mitochondrial DNA results in a precise excision of the terminal intron of the cytochrome b gene. J Biol Chem. 1985;260:3235-8 pubmed
    ..gene CBP2 was previously proposed to code for a protein necessary for processing of the terminal intron in the cytochrome b pre-mRNA (McGraw, P., and Tzagoloff, A. (1983) J. Biol. Chem. 258, 9459-9468)...
  4. Kreike J, Bechmann H, Van Hemert F, Schweyen R, Boer P, Kaudewitz F, et al. The identification of apocytochrome b as a mitochondrial gene product and immunological evidence for altered apocytochrome b in yeast strains having mutations in the COB region of mitochondrial DNA. Eur J Biochem. 1979;101:607-17 pubmed
    ..New mitochondrial translation products in 36 strains of Saccharomyces cerevisiae having mutations in the COB region of the mitochondrial DNA, are precipitated by this antiserum...
  5. Webb A, Rose M, Westhof E, Weeks K. Protein-dependent transition states for ribonucleoprotein assembly. J Mol Biol. 2001;309:1087-100 pubmed
    ..4) Because assembly from the kinetically trapped state is faster at elevated temperature, we can identify conditions where CYT-18 accelerates (catalyzes) bI5 RNA folding relative to assembly with CBP2. ..
  6. Keil M, Bareth B, Woellhaf M, Peleh V, Prestele M, Rehling P, et al. Oxa1-ribosome complexes coordinate the assembly of cytochrome C oxidase in mitochondria. J Biol Chem. 2012;287:34484-93 pubmed publisher
    ..This points to a role for Oxa1 in the spatial coordination of the ribosome with assembly factors that are critical for enzyme biogenesis. ..
  7. Brivet Chevillotte P, di Rago J. Electron-transfer restoration by vitamin K3 in a complex III-deficient mutant of S. cerevisiae and sequence of the corresponding cytochrome b mutation. FEBS Lett. 1989;255:5-9 pubmed
    The yeast box-mutant W7 exhibits deficiencies in cytochrome b and in nuclear coded complex III subunits, a phenotype observed previously in a patient with mitochondrial myopathy...
  8. Vallieres C, Trouillard M, Dujardin G, Meunier B. Deleterious effect of the Qo inhibitor compound resistance-conferring mutation G143A in the intron-containing cytochrome b gene and mechanisms for bypassing it. Appl Environ Microbiol. 2011;77:2088-93 pubmed publisher
    The mutation G143A in the inhibitor binding site of cytochrome b confers a high level of resistance to fungicides targeting the bc(1) complex...
  9. Shingu Vazquez M, Camacho Villasana Y, Sandoval Romero L, Butler C, Fox T, Perez Martinez X. The carboxyl-terminal end of Cox1 is required for feedback assembly regulation of Cox1 synthesis in Saccharomyces cerevisiae mitochondria. J Biol Chem. 2010;285:34382-9 pubmed publisher
    ..Our data suggest a novel activity of Pet54 required for normal synthesis of Cox1 that is independent of the Cox1 C-terminal end. ..
  10. Ding M, di Rago J, Trumpower B. Combining Inhibitor Resistance-conferring Mutations in Cytochrome b Creates Conditional Synthetic Lethality in Saccharomyces cerevisiae. J Biol Chem. 2009;284:8478-85 pubmed publisher
    ..Its core subunit, cytochrome b, contains two sites, center P and center N, that participate in the electron transfer activity of the bc(1) ..
  11. Seddiki N, Meunier B, Lemesle Meunier D, Brasseur G. Is cytochrome b glutamic acid 272 a quinol binding residue in the bc1 complex of Saccharomyces cerevisiae?. Biochemistry. 2008;47:2357-68 pubmed publisher
    ..The data lead us to suggest that E272 may be involved in a later step on the proton exit pathway via the interaction with a water molecule. ..
  12. Cui T, Conte A, Fox J, Zara V, Winge D. Modulation of the respiratory supercomplexes in yeast: enhanced formation of cytochrome oxidase increases the stability and abundance of respiratory supercomplexes. J Biol Chem. 2014;289:6133-41 pubmed publisher
    ..This study presents the first evidence showing that supercomplexes can be stabilized by the stimulated formation of CcO. ..
  13. Asher E, Groudinsky O, Dujardin G, Altamura N, Kermorgant M, Slonimski P. Novel class of nuclear genes involved in both mRNA splicing and protein synthesis in Saccharomyces cerevisiae mitochondria. Mol Gen Genet. 1989;215:517-28 pubmed
    ..three distinct nuclear genes, NAM1, NAM7, and NAM8, which alleviate mitochondrial intron mutations of the cytochrome b and COXI (subunit I of cytochrome oxidase) genes when present on multicopy plasmids...
  14. Chen W, Islas Osuna M, Dieckmann C. Suppressor analysis of mutations in the 5'-untranslated region of COB mRNA identifies components of general pathways for mitochondrial mRNA processing and decay in Saccharomyces cerevisiae. Genetics. 1999;151:1315-25 pubmed
    The cytochrome b gene in Saccharomyces cerevisiae, COB, is encoded by the mitochondrial genome. Nuclear-encoded Cbp1 protein is required specifically for COB mRNA stabilization...
  15. Nobrega F, Tzagoloff A. Assembly of the mitochondrial membrane system. DNA sequence and organization of the cytochrome b gene in Saccharomyces cerevisiae D273-10B. J Biol Chem. 1980;255:9828-37 pubmed
    ..of cytoplasmic "petite" (rho-) mutants of Saccharomyces cerevisiae have been used to sequence the cytochrome b gene. A continuous sequence of 6.2 kilobase pairs has been obtained from 71.4 to 80...
  16. Conte A, Papa B, Ferramosca A, Zara V. The dimerization of the yeast cytochrome bc1 complex is an early event and is independent of Rip1. Biochim Biophys Acta. 2015;1853:987-95 pubmed publisher
    ..Finally, a new model of cytochrome bc1 complex assembly, in which distinct intermediates sequentially interact during bc1 maturation, has been proposed. ..
  17. Wenz T, Covian R, Hellwig P, MacMillan F, Meunier B, Trumpower B, et al. Mutational analysis of cytochrome b at the ubiquinol oxidation site of yeast complex III. J Biol Chem. 2007;282:3977-88 pubmed
    ..activities and pre-steady-state reduction kinetics of yeast cytochrome bc1 complexes with mutations in cytochrome b that we expected would affect oxidation of ubiquinol...
  18. Covian R, Trumpower B. Rapid electron transfer between monomers when the cytochrome bc1 complex dimer is reduced through center N. J Biol Chem. 2005;280:22732-40 pubmed
    We have obtained evidence for electron transfer between cytochrome b subunits of the yeast bc(1) complex dimer by analyzing pre-steady state reduction of cytochrome b in the presence of center P inhibitors...
  19. Lange C, Hunte C. Crystal structure of the yeast cytochrome bc1 complex with its bound substrate cytochrome c. Proc Natl Acad Sci U S A. 2002;99:2800-5 pubmed
    ..Obviously, cytochrome c reduction by the cytochrome bc1 complex can be regulated in response to respiratory conditions. ..
  20. Claros M, Perea J, Shu Y, Samatey F, Popot J, Jacq C. Limitations to in vivo import of hydrophobic proteins into yeast mitochondria. The case of a cytoplasmically synthesized apocytochrome b. Eur J Biochem. 1995;228:762-71 pubmed
    ..Such features may have played a major role in the evolution of mitochondrial genomes. ..
  21. Foury F, Roganti T, Lecrenier N, Purnelle B. The complete sequence of the mitochondrial genome of Saccharomyces cerevisiae. FEBS Lett. 1998;440:325-31 pubmed
    ..Our data suggest that shuffling of mobile elements plays an important role in the remodelling of the yeast mitochondrial genome. ..