CLB5

Summary

Gene Symbol: CLB5
Description: B-type cyclin CLB5
Alias: B-type cyclin CLB5
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Archambault V, Buchler N, Wilmes G, Jacobson M, Cross F. Two-faced cyclins with eyes on the targets. Cell Cycle. 2005;4:125-30 pubmed
    We recently reported that the 'hydrophobic patch' (HP) of the Saccharomyces cerevisiae S-phase cyclin Clb5 facilitates its interaction with Orc6 (via its Cy or RXL motif), providing a mechanism that helps prevent rereplication from ..
  2. Kõivomägi M, Valk E, Venta R, Iofik A, Lepiku M, Balog E, et al. Cascades of multisite phosphorylation control Sic1 destruction at the onset of S phase. Nature. 2011;480:128-31 pubmed publisher
    ..S phase is thought to be governed by complexes of Cdk1 and Cln cyclins that phosphorylate six or more sites on the Clb5-Cdk1 inhibitor Sic1, directing it to SCF-mediated destruction...
  3. Schwob E, Bohm T, Mendenhall M, Nasmyth K. The B-type cyclin kinase inhibitor p40SIC1 controls the G1 to S transition in S. cerevisiae. Cell. 1994;79:233-44 pubmed
    ..In wild-type cells, p40SIC1 protein appears at the end of mitosis and disappears shortly before S phase. Proteolysis of a cyclin-specific inhibitor of Cdc28 is therefore an essential aspect of the G1 to S phase transition. ..
  4. Rice L, Plakas C, Nickels J. Loss of meiotic rereplication block in Saccharomyces cerevisiae cells defective in Cdc28p regulation. Eukaryot Cell. 2005;4:55-62 pubmed
    ..Finally, we present evidence of a possible role for a Cdc55p-dependent protein phosphatase 2A in initiating meiotic replication. ..
  5. Stuart D, Wittenberg C. CLB5 and CLB6 are required for premeiotic DNA replication and activation of the meiotic S/M checkpoint. Genes Dev. 1998;12:2698-710 pubmed
    ..The B-type cyclins Clb5 and Clb6 are the primary activators of the S phase function of the budding yeast CDK Cdc28...
  6. Segal M, Clarke D, Reed S. Clb5-associated kinase activity is required early in the spindle pathway for correct preanaphase nuclear positioning in Saccharomyces cerevisiae. J Cell Biol. 1998;143:135-45 pubmed
    ..By this approach, a novel phenotype associated with CLB5 mutation was observed. Homozygous cdc28-4(ts) clb5 diploids were inviable at room temperature...
  7. Gibson D, Aparicio J, Hu F, Aparicio O. Diminished S-phase cyclin-dependent kinase function elicits vital Rad53-dependent checkpoint responses in Saccharomyces cerevisiae. Mol Cell Biol. 2004;24:10208-22 pubmed
    ..In Saccharomyces cerevisiae, the Clb5 and Clb6 cyclins activate Cdk1 and drive replication origin firing...
  8. Chen S, Bell S. CDK prevents Mcm2-7 helicase loading by inhibiting Cdt1 interaction with Orc6. Genes Dev. 2011;25:363-72 pubmed publisher
  9. Smith K, Penkner A, Ohta K, Klein F, Nicolas A. B-type cyclins CLB5 and CLB6 control the initiation of recombination and synaptonemal complex formation in yeast meiosis. Curr Biol. 2001;11:88-97 pubmed
    ..how replication influences the initiation of recombination, we have used mutations in the B-type cyclin genes CLB5 and CLB6, which specifically prevent premeiotic replication in the yeast Saccharomyces cerevisiae...

More Information

Publications78

  1. Hsu W, Erickson S, Tsai H, Andrews C, Vas A, Clarke D. S-phase cyclin-dependent kinases promote sister chromatid cohesion in budding yeast. Mol Cell Biol. 2011;31:2470-83 pubmed publisher
    ..that promote cohesion within S phase in the absence of Pds1 and found that cells lacking the S-phase cyclins Clb5 and Clb6 have a cohesion defect under conditions of replication stress...
  2. Ikui A, Archambault V, Drapkin B, Campbell V, Cross F. Cyclin and cyclin-dependent kinase substrate requirements for preventing rereplication reveal the need for concomitant activation and inhibition. Genetics. 2007;175:1011-22 pubmed
    ..for cyclin specificity in the use of different mechanisms to control rereplication: both the S-phase cyclin Clb5 and the mitotic cyclins Clb1-4 were inferred to be capable of imposing ORC-based and MCM-based controls...
  3. Huisman S, Smeets M, Segal M. Phosphorylation of Spc110p by Cdc28p-Clb5p kinase contributes to correct spindle morphogenesis in S. cerevisiae. J Cell Sci. 2007;120:435-46 pubmed
    ..Together, our results point to the function of cyclin-dependent kinase phosphorylation of Spc110p and provide, in addition, support to a model for Clb5p control of spindle polarity at the level of astral microtubule organisation. ..
  4. Cross F, Jacobson M. Conservation and function of a potential substrate-binding domain in the yeast Clb5 B-type cyclin. Mol Cell Biol. 2000;20:4782-90 pubmed
    ..These results support the idea of a modular, structurally conserved cyclin domain involved in substrate targeting. ..
  5. Shirayama M, Toth A, Galova M, Nasmyth K. APC(Cdc20) promotes exit from mitosis by destroying the anaphase inhibitor Pds1 and cyclin Clb5. Nature. 1999;402:203-7 pubmed
    ..allows activation of Cdc14 and promotes exit from mitosis by mediating proteolysis of Pds1 and the S phase cyclin Clb5 in the yeast Saccharomyces cerevisiae...
  6. Verma R, Feldman R, Deshaies R. SIC1 is ubiquitinated in vitro by a pathway that requires CDC4, CDC34, and cyclin/CDK activities. Mol Biol Cell. 1997;8:1427-37 pubmed
    ..The complementary C-terminal segment of SIC1 binds to the S-phase cyclin CLB5, indicating a modular structure for SIC1.
  7. Bhaduri S, Pryciak P. Cyclin-specific docking motifs promote phosphorylation of yeast signaling proteins by G1/S Cdk complexes. Curr Biol. 2011;21:1615-23 pubmed publisher
    ..These docking sites, which are distinct from Clb5/cyclin A-binding "RXL" motifs, bind preferentially to Cln2...
  8. Woodbury E, Morgan D. Cdk and APC activities limit the spindle-stabilizing function of Fin1 to anaphase. Nat Cell Biol. 2007;9:106-12 pubmed
    ..Phosphorylation of Fin1 from S phase to metaphase, by the cyclin-dependent kinase Clb5-Cdk1, inhibits Fin1 association with the spindle...
  9. Cross F, Schroeder L, Bean J. Phosphorylation of the Sic1 inhibitor of B-type cyclins in Saccharomyces cerevisiae is not essential but contributes to cell cycle robustness. Genetics. 2007;176:1541-55 pubmed
    ..SIC1-0P was lethal when combined with deletion of CLB2, CLB3, or CLB5, the major B-type cyclins...
  10. Donaldson A, Raghuraman M, Friedman K, Cross F, Brewer B, Fangman W. CLB5-dependent activation of late replication origins in S. cerevisiae. Mol Cell. 1998;2:173-82 pubmed
    ..We show that the B-type cyclins are required for proper execution of this temporal program. clb5 cells activate early origins but not late origins, explaining the previously described long clb5 S phase...
  11. Barberis M, Linke C, Adrover M, Gonzalez Novo A, Lehrach H, Krobitsch S, et al. Sic1 plays a role in timing and oscillatory behaviour of B-type cyclins. Biotechnol Adv. 2012;30:108-30 pubmed publisher
    ..Our results highlight an additional role for Sic1 in regulating Cdk1-Clb complexes, coordinating their appearance. ..
  12. Segal M, Clarke D, Maddox P, Salmon E, Bloom K, Reed S. Coordinated spindle assembly and orientation requires Clb5p-dependent kinase in budding yeast. J Cell Biol. 2000;148:441-52 pubmed
    ..In contrast, B-type cyclins, Clb3p and Clb4p, though partially redundant with Clb5p for an early role in spindle morphogenesis, preferentially promote spindle assembly. ..
  13. Ikui A, Cross F. Specific genetic interactions between spindle assembly checkpoint proteins and B-Type cyclins in Saccharomyces cerevisiae. Genetics. 2009;183:51-61 pubmed publisher
    The B-type cyclin Clb5 is involved primarily in control of DNA replication in Saccharomyces cerevisiae...
  14. Wilmes G, Archambault V, Austin R, Jacobson M, Bell S, Cross F. Interaction of the S-phase cyclin Clb5 with an "RXL" docking sequence in the initiator protein Orc6 provides an origin-localized replication control switch. Genes Dev. 2004;18:981-91 pubmed
    Cyclin-dependent kinases are critical regulators of eukaryotic DNA replication. We show that the S-phase cyclin Clb5 binds stably and directly to the origin recognition complex (ORC)...
  15. Loog M, Morgan D. Cyclin specificity in the phosphorylation of cyclin-dependent kinase substrates. Nature. 2005;434:104-8 pubmed
    ..Here we compare the specificity of two budding yeast cyclins, the S-phase cyclin Clb5 and the M-phase cyclin Clb2, in the phosphorylation of 150 Cdk1 (Cdc28) substrates...
  16. Verma R, McDonald H, Yates J, Deshaies R. Selective degradation of ubiquitinated Sic1 by purified 26S proteasome yields active S phase cyclin-Cdk. Mol Cell. 2001;8:439-48 pubmed
    ..Activation of S-Cdk reported herein represents a complete reconstitution of the regulatory switch underlying the G1/S transition in budding yeast. ..
  17. Schwob E, Nasmyth K. CLB5 and CLB6, a new pair of B cyclins involved in DNA replication in Saccharomyces cerevisiae. Genes Dev. 1993;7:1160-75 pubmed
    ..We describe a new family of B-type cyclin genes, CLB5 and CLB6, whose transcripts appear in late G1 along with those of CLN1, CLN2, and many genes required for DNA ..
  18. Koren A, Soifer I, Barkai N. MRC1-dependent scaling of the budding yeast DNA replication timing program. Genome Res. 2010;20:781-90 pubmed publisher
    ..Mrc1 emerges as a regulator of this robustness of the replication program. ..
  19. Vincent J, Kwong T, Tsukiyama T. ATP-dependent chromatin remodeling shapes the DNA replication landscape. Nat Struct Mol Biol. 2008;15:477-84 pubmed publisher
    ..These findings identify ATP-dependent chromatin-remodeling complexes that promote DNA replication and define a specific stage of replication that requires remodeling for normal function. ..
  20. Devault A, Gueydon E, Schwob E. Interplay between S-cyclin-dependent kinase and Dbf4-dependent kinase in controlling DNA replication through phosphorylation of yeast Mcm4 N-terminal domain. Mol Biol Cell. 2008;19:2267-77 pubmed publisher
    ..Deletion of the S phase cyclins Clb5,6 is synthetic lethal with mcm4-5A and mimics its effects on DDK up mutants...
  21. Casey L, Patterson E, Muller U, Fox C. Conversion of a replication origin to a silencer through a pathway shared by a Forkhead transcription factor and an S phase cyclin. Mol Biol Cell. 2008;19:608-22 pubmed
    ..expression of FKH1 (FKH1(hc)), a conserved forkhead transcription factor, or by deletion of the S phase cyclin CLB5 (clb5Delta)...
  22. Pope P, Bhaduri S, Pryciak P. Regulation of cyclin-substrate docking by a G1 arrest signaling pathway and the Cdk inhibitor Far1. Curr Biol. 2014;24:1390-1396 pubmed publisher
    ..Our findings uncover a novel mechanism of Cdk regulation by external signals and shed new light on Far1 function to provide a revised view of cell-cycle arrest in this model system. ..
  23. Ebrahimi H, Donaldson A. Release of yeast telomeres from the nuclear periphery is triggered by replication and maintained by suppression of Ku-mediated anchoring. Genes Dev. 2008;22:3363-74 pubmed publisher
    ..In addition to maintaining genetic information, DNA replication may therefore regulate subnuclear organization of chromatin. ..
  24. Decesare J, Stuart D. Among B-type cyclins only CLB5 and CLB6 promote premeiotic S phase in Saccharomyces cerevisiae. Genetics. 2012;190:1001-16 pubmed publisher
    The Saccharomyces cerevisiae cyclin Clb5 is required for premeiotic S phase, meiotic recombination, and successful progression through meiosis...
  25. Howe A, Fairn G, Macdonald K, Bankaitis V, McMaster C. Regulation of phosphoinositide levels by the phospholipid transfer protein Sec14p controls Cdc42p/p21-activated kinase-mediated cell cycle progression at cytokinesis. Eukaryot Cell. 2007;6:1814-23 pubmed
    ..Sec14p regulation of phosphoinositide levels affects cytokinesis at the level of the Cdc42p/Cla4p/Ste20p signaling cascade. ..
  26. Dhillon N, Oki M, Szyjka S, Aparicio O, Kamakaka R. H2A.Z functions to regulate progression through the cell cycle. Mol Cell Biol. 2006;26:489-501 pubmed
    ..We also found that H2A.Z localized to the promoters of cyclin genes, and cells lacking H2A.Z were delayed in the induction of these cyclin genes. Several different models are proposed to explain these observations. ..
  27. Pic Taylor A, Darieva Z, Morgan B, Sharrocks A. Regulation of cell cycle-specific gene expression through cyclin-dependent kinase-mediated phosphorylation of the forkhead transcription factor Fkh2p. Mol Cell Biol. 2004;24:10036-46 pubmed
    ..Our data therefore identify an important regulatory target for cyclin-dependent kinases in the cell cycle and further our molecular understanding of the key cell cycle regulatory transcription factor Fkh2p. ..
  28. Tran L, Wang ondu R, Weng J, Wanjiku G, Fong C, Kile A, et al. TORC1 kinase and the S-phase cyclin Clb5 collaborate to promote mitotic spindle assembly and DNA replication in S. cerevisiae. Curr Genet. 2010;56:479-93 pubmed publisher
    ..We investigated the rapamycin-hypersensitivity phenotype of cells lacking the S phase cyclin Clb5 (clb5?) as a basis for uncovering novel connections between TORC1 and the cell cycle regulatory machinery...
  29. Sari F, Braus G, Irniger S. A process independent of the anaphase-promoting complex contributes to instability of the yeast S phase cyclin Clb5. J Biol Chem. 2007;282:26614-22 pubmed
    ..In the budding yeast Saccharomyces cerevisiae, the S phase cyclin Clb5 and the mitotic cyclins Clb1-4 are known as substrates of this complex...
  30. Venta R, Valk E, Kõivomägi M, Loog M. Double-negative feedback between S-phase cyclin-CDK and CKI generates abruptness in the G1/S switch. Front Physiol. 2012;3:459 pubmed publisher
    ..by phosphorylation of the Cdk1 inhibitor Sic1 at multiple sites by G1-phase CDK (Cln1,2-Cdk1) and S-phase CDK (Clb5,6-Cdk1) complexes...
  31. Queralt E, Igual J. Functional connection between the Clb5 cyclin, the protein kinase C pathway and the Swi4 transcription factor in Saccharomyces cerevisiae. Genetics. 2005;171:1485-98 pubmed
    The rsf12 mutation was isolated in a synthetic lethal screen for genes functionally interacting with Swi4. RSF12 is CLB5. The clb5 swi4 mutant cells arrest at G(2)/M due to the activation of the DNA-damage checkpoint...
  32. Lyons N, Morgan D. Cdk1-dependent destruction of Eco1 prevents cohesion establishment after S phase. Mol Cell. 2011;42:378-89 pubmed publisher
    ..We also show that deregulation of Eco1 results in chromosome separation defects in anaphase. We conclude that this regulatory mechanism helps optimize the level of sister chromatid cohesion, ensuring a robust and efficient anaphase. ..
  33. McCune H, Danielson L, Alvino G, Collingwood D, Delrow J, Fangman W, et al. The temporal program of chromosome replication: genomewide replication in clb5{Delta} Saccharomyces cerevisiae. Genetics. 2008;180:1833-47 pubmed publisher
  34. Yaakov G, Duch A, Garcia Rubio M, Clotet J, Jimenez J, Aguilera A, et al. The stress-activated protein kinase Hog1 mediates S phase delay in response to osmostress. Mol Biol Cell. 2009;20:3572-82 pubmed publisher
    ..early S phase, the SAPK prevents firing of replication origins by delaying the accumulation of the S phase cyclins Clb5 and Clb6...
  35. Pessina S, Tsiarentsyeva V, Busnelli S, Vanoni M, Alberghina L, Coccetti P. Snf1/AMPK promotes S-phase entrance by controlling CLB5 transcription in budding yeast. Cell Cycle. 2010;9:2189-200 pubmed
    ..The lack of Snf1 ?-catalytic subunit down-regulates the growth rate and CLB5 expression, delaying Sld2 phosphorylation and G 1/S transition, in cells grown in 2%, but not in 5% glucose...
  36. Campsteijn C, Wijnands Collin A, Logie C. Reverse genetic analysis of the yeast RSC chromatin remodeler reveals a role for RSC3 and SNF5 homolog 1 in ploidy maintenance. PLoS Genet. 2007;3:e92 pubmed
    ..deregulation of SFH1 and RSC3 to induce these ploidy shifts was eliminated by knockout of the S-phase cyclin CLB5 and by transient depletion of replication origin licensing factor Cdc6p...
  37. Wasch R, Cross F. APC-dependent proteolysis of the mitotic cyclin Clb2 is essential for mitotic exit. Nature. 2002;418:556-62 pubmed
    ..Mitotic exit was proposed to require degradation of the S phase cyclin Clb5 by the anaphase-promoting complex activated by Cdc20 (APC(Cdc20))...
  38. Kito K, Kawaguchi N, Okada S, Ito T. Discrimination between stable and dynamic components of protein complexes by means of quantitative proteomics. Proteomics. 2008;8:2366-70 pubmed publisher
    ..We successfully applied this strategy to analyze two yeast protein complexes, eIF2B-eIF2 and cyclin-Cdc28. ..
  39. Vallen E, Cross F. Interaction between the MEC1-dependent DNA synthesis checkpoint and G1 cyclin function in Saccharomyces cerevisiae. Genetics. 1999;151:459-71 pubmed
    ..Wild-type levels of CLN1 and CLN2, or overexpression of CLN1, CLN2, or CLB5, but not CLN3, killed mec1 strains...
  40. Manfrini N, Gobbini E, Baldo V, Trovesi C, Lucchini G, Longhese M. G(1)/S and G(2)/M cyclin-dependent kinase activities commit cells to death in the absence of the S-phase checkpoint. Mol Cell Biol. 2012;32:4971-85 pubmed publisher
    ..Moreover, these findings suggest that the essential function of Mec1 and Rad53 is not necessarily separated from the function of these kinases in supporting DNA synthesis under stress conditions. ..
  41. Moreno Torres M, Jaquenoud M, Péli Gulli M, Nicastro R, De Virgilio C. TORC1 coordinates the conversion of Sic1 from a target to an inhibitor of cyclin-CDK-Cks1. Cell Discov. 2017;3:17012 pubmed publisher
    ..as a specific docking site for the CDK phospho-acceptor subunit Cks1 that sequesters, together with a C-terminal Clb5-binding motif in Sic1, Clb5-CDK-Cks1 complexes, thereby preventing them from flagging Sic1 for ubiquitin-dependent ..
  42. Ikui A, Rossio V, Schroeder L, Yoshida S. A yeast GSK-3 kinase Mck1 promotes Cdc6 degradation to inhibit DNA re-replication. PLoS Genet. 2012;8:e1003099 pubmed publisher
    ..We propose that both CDK and Mck1 kinases are required for Cdc6 degradation to ensure a tight control of DNA replication. ..
  43. Lu D, Hsiao J, Davey N, Van Voorhis V, Foster S, Tang C, et al. Multiple mechanisms determine the order of APC/C substrate degradation in mitosis. J Cell Biol. 2014;207:23-39 pubmed publisher
    ..Degradation of the S cyclin, Clb5, begins early in mitosis, followed 6 min later by the degradation of securin and Dbf4...
  44. Miao Y, Han X, Zheng L, Xie Y, Mu Y, Yates J, et al. Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast. Nat Commun. 2016;7:11265 pubmed publisher
    ..Overall, this work identifies fimbrin as a key target for cell cycle regulation of actin cable assembly in budding yeast, and suggests an underlying mechanism. ..
  45. Moore J, D Silva S, Miller R. The CLIP-170 homologue Bik1p promotes the phosphorylation and asymmetric localization of Kar9p. Mol Biol Cell. 2006;17:178-91 pubmed
    ..Furthermore, the two-hybrid interaction between full-length KAR9 and the cyclin CLB5 requires BIK1...
  46. Lu D, Girard J, Li W, Mizrak A, Morgan D. Quantitative framework for ordered degradation of APC/C substrates. BMC Biol. 2015;13:96 pubmed publisher
    ..We show experimentally that increased expression of the early APC/C(Cdc20) substrate Clb5 does not delay the degradation of the later substrate securin, arguing against a role for competition with Clb5 in ..
  47. Maekawa H, Usui T, Knop M, Schiebel E. Yeast Cdk1 translocates to the plus end of cytoplasmic microtubules to regulate bud cortex interactions. EMBO J. 2003;22:438-49 pubmed
    ..Cdc28 activity is required to concentrate Kar9 at the plus end of CMs and hence to establish contacts with the bud cortex. The Cdc28-regulated localization of Kar9 is therefore an integral part of the program that aligns spindles. ..
  48. Kuhne C, Linder P. A new pair of B-type cyclins from Saccharomyces cerevisiae that function early in the cell cycle. EMBO J. 1993;12:3437-47 pubmed
    Two new B-type cyclin genes from Saccharomyces cerevisiae, called CLB5 and CLB6, are located in a tail to tail arrangement adjacent to the G2/M phase promoting cyclins CLB2 and CLB1, respectively...
  49. Li X, Cai M. Recovery of the yeast cell cycle from heat shock-induced G(1) arrest involves a positive regulation of G(1) cyclin expression by the S phase cyclin Clb5. J Biol Chem. 1999;274:24220-31 pubmed
    ..Here we report our finding that the G(1) cyclin Cln3 and the S cyclin Clb5 are the key factors required for recovery from heat shock-induced G(1) arrest...
  50. Cherkasova V, Lyons D, Elion E. Fus3p and Kss1p control G1 arrest in Saccharomyces cerevisiae through a balance of distinct arrest and proliferative functions that operate in parallel with Far1p. Genetics. 1999;151:989-1004 pubmed
    ..Fus3p and Kss1p together promote G1 arrest by repressing transcription of G1/S cyclin genes (CLN1, CLN2, CLB5) by a mechanism that blocks their activation by Cln3p/Cdc28p kinase...
  51. Sollier J, Lin W, Soustelle C, Suhre K, Nicolas A, Geli V, et al. Set1 is required for meiotic S-phase onset, double-strand break formation and middle gene expression. EMBO J. 2004;23:1957-67 pubmed
    ..Our results unveil multiple roles of Set1 in meiotic differentiation and distinguish between HMTase-dependent and -independent Set1 functions. ..
  52. Cui Z, Horecka J, Jigami Y. Cdc4 is involved in the transcriptional control of OCH1, a gene encoding alpha-1,6-mannosyltransferase in Saccharomyces cerevisiae. Yeast. 2002;19:69-77 pubmed
    ..The cdc4(bon) mutant is partially suppressed by CLB5 overexpression and accumulates Sic1 protein, indicating that OCH1 transcription is controlled by the ubiquitin-..
  53. Ostapenko D, Burton J, Solomon M. The Ubp15 deubiquitinase promotes timely entry into S phase in Saccharomyces cerevisiae. Mol Biol Cell. 2015;26:2205-16 pubmed publisher
    ..Both phenotypes of ubp15∆ cells were rescued by additional copies of the S-phase cyclin gene CLB5. Clb5 is an unstable protein targeted for proteasome-mediated degradation by several pathways...
  54. Corbi D, Sunder S, Weinreich M, Skokotas A, Johnson E, Winter E. Multisite phosphorylation of the Sum1 transcriptional repressor by S-phase kinases controls exit from meiotic prophase in yeast. Mol Cell Biol. 2014;34:2249-63 pubmed publisher
    ..Here, we show that the S-phase-promoting cyclin Clb5 activates Cdk1 to phosphorylate most, and perhaps all, of the 11 minimal cyclin-dependent kinase (CDK) phospho-..
  55. Newnham L, Jordan P, Carballo J, Newcombe S, Hoffmann E. Ipl1/Aurora kinase suppresses S-CDK-driven spindle formation during prophase I to ensure chromosome integrity during meiosis. PLoS ONE. 2013;8:e83982 pubmed publisher
    ..Here we uncover a novel and unexpected role for Aurora kinase in preventing the formation of spindles by Clb5-CDK (S-CDK) during meiotic prophase I and when the DDR is active in budding yeast...
  56. Schreiber G, Barberis M, Scolari S, Klaus C, Herrmann A, Klipp E. Unraveling interactions of cell cycle-regulating proteins Sic1 and B-type cyclins in living yeast cells: a FLIM-FRET approach. FASEB J. 2012;26:546-54 pubmed publisher
    ..Specifically, Sic1/Clb3 interaction was observed for the first time, and Sic1/Clb2 and Sic1/Clb5 pairs were confirmed, but no Sic1/Clb4 interaction was found, which suggests that, despite high functional homology ..
  57. Palou G, Palou R, Guerra Moreno A, Duch A, Travesa A, Quintana D. Cyclin regulation by the s phase checkpoint. J Biol Chem. 2010;285:26431-40 pubmed publisher
    ..We show here that the S phase cyclins Clb5 and Clb6 are regulated in response to genotoxic stress in the budding yeast Saccharomyces cerevisiae...
  58. Hotz M, Lengefeld J, Barral Y. The MEN mediates the effects of the spindle assembly checkpoint on Kar9-dependent spindle pole body inheritance in budding yeast. Cell Cycle. 2012;11:3109-16 pubmed publisher
    ..Inactivation of the B-type cyclin Clb5 led to a SAC-dependent defect in Kar9 orientation and SPB segregation...
  59. Meyn M, Holloway S. S-phase cyclins are required for a stable arrest at metaphase. Curr Biol. 2000;10:1599-602 pubmed
    ..Here we show that the S-phase cyclins Clb5 and Clb6 are required for this arrest. Strains lacking Clb5 and Clb6 are hypersensitive to DNA damage...
  60. Juanes M, ten Hoopen R, Segal M. Ase1p phosphorylation by cyclin-dependent kinase promotes correct spindle assembly in S. cerevisiae. Cell Cycle. 2011;10:1988-97 pubmed
    ..Indeed, Ase1p phosphorylation coincident with spindle assembly requires Clb5p, Clb3p and Clb4p. Moreover, in clb5? cells, Ase1p recruitment and the kinetics of spindle formation were perturbed...
  61. Jeffery D, Kakusho N, You Z, Gharib M, Wyse B, Drury E, et al. CDC28 phosphorylates Cac1p and regulates the association of chromatin assembly factor I with chromatin. Cell Cycle. 2015;14:74-85 pubmed publisher
    ..Hence, we implicate CDC28 in the regulation of chromatin reassembly during DNA replication. These findings provide novel mechanistic insights on the links between cell-cycle regulation, DNA replication and chromatin reassembly. ..
  62. Chen X, Niu H, Yu Y, Wang J, Zhu S, Zhou J, et al. Enrichment of Cdk1-cyclins at DNA double-strand breaks stimulates Fun30 phosphorylation and DNA end resection. Nucleic Acids Res. 2016;44:2742-53 pubmed publisher
    ..that Cdk1 and multiple cyclins become highly enriched at DSBs and that the recruitment of Cdk1 and cyclins Clb2 and Clb5 ensures optimal Fun30 phosphorylation and checkpoint activation...
  63. Jackson L, Reed S, Haase S. Distinct mechanisms control the stability of the related S-phase cyclins Clb5 and Clb6. Mol Cell Biol. 2006;26:2456-66 pubmed
    The yeast S-phase cyclins Clb5 and Clb6 are closely related proteins that are synthesized late in G1...
  64. Crasta K, Huang P, Morgan G, Winey M, Surana U. Cdk1 regulates centrosome separation by restraining proteolysis of microtubule-associated proteins. EMBO J. 2006;25:2551-63 pubmed
  65. Caldwell J, Chen Y, Schollaert K, Theis J, Babcock G, Newlon C, et al. Orchestration of the S-phase and DNA damage checkpoint pathways by replication forks from early origins. J Cell Biol. 2008;180:1073-86 pubmed publisher
    ..Thus, oncogene-mediated deregulation of cyclins in the early stages of cancer development could contribute to genomic instability through a deficiency in the forks required to establish the S-phase checkpoint. ..
  66. Laman H, Balderes D, Shore D. Disturbance of normal cell cycle progression enhances the establishment of transcriptional silencing in Saccharomyces cerevisiae. Mol Cell Biol. 1995;15:3608-17 pubmed
    ..Mutations in different cyclin genes (CLN3, CLB5, and CLB2) and two cell cycle transcriptional regulators (SWI4 and MBP1) also suppress the silencing defect at HMR...
  67. Haber J, Braberg H, Wu Q, Alexander R, Haase J, Ryan C, et al. Systematic triple-mutant analysis uncovers functional connectivity between pathways involved in chromosome regulation. Cell Rep. 2013;3:2168-78 pubmed publisher
    ..A similar TMA was carried out after deleting both CLB5 and CLB6, cyclins that regulate DNA replication, revealing a strong functional connection to chromosome segregation...
  68. Trelles Sticken E, Bonfils S, Sollier J, Geli V, Scherthan H, de La Roche Saint André C. Set1- and Clb5-deficiencies disclose the differential regulation of centromere and telomere dynamics in Saccharomyces cerevisiae meiosis. J Cell Sci. 2005;118:4985-94 pubmed
    ..Absence of the B-type cyclin Clb5 or the Set1 histone methyltransferase leads to a delay of premeiotic S phase by separate mechanisms...
  69. Schwab M, Neutzner M, Mocker D, Seufert W. Yeast Hct1 recognizes the mitotic cyclin Clb2 and other substrates of the ubiquitin ligase APC. EMBO J. 2001;20:5165-75 pubmed
    ..We propose that proteins of the Cdc20 family are substrate recognition subunits of the ubiquitin ligase APC. ..