Gene Symbol: CKB2
Description: casein kinase 2 regulatory subunit CKB2
Alias: casein kinase 2 regulatory subunit CKB2
Species: Saccharomyces cerevisiae S288c
Products:     CKB2

Top Publications

  1. Peng Y, Wong C, Nakajima Y, Tyers R, Sarkeshik A, Yates J, et al. Overlapping kinetochore targets of CK2 and Aurora B kinases in mitotic regulation. Mol Biol Cell. 2011;22:2680-9 pubmed publisher
    ..Furthermore, we show Mif2 and the inner kinetochore protein Ndc10 are phosphorylated by CK2, and this phosphorylation plays antagonistic and synergistic roles with Aurora B phosphorylation of these targets, respectively. ..
  2. Tripodi F, Cirulli C, Reghellin V, Marin O, Brambilla L, Schiappelli M, et al. CK2 activity is modulated by growth rate in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2010;398:44-50 pubmed publisher
    ..Also in chemostat cultures nuclear CK2 activity is higher in faster growing cells providing the first unequivocal demonstration that growth rate itself can affect CK2 activity in a eukaryotic organism. ..
  3. Kubinski K, Zielinski R, Hellman U, Mazur E, Szyszka R. Yeast elf1 factor is phosphorylated and interacts with protein kinase CK2. J Biochem Mol Biol. 2006;39:311-8 pubmed
    ..These data may help to elucidate the role of protein kinase CK2 and Elf1 in the regulation of transcription elongation. ..
  4. Masłyk M, Kochanowicz E, Zieliński R, Kubinski K, Hellman U, Szyszka R. Yeast surviving factor Svf1 as a new interacting partner, regulator and in vitro substrate of protein kinase CK2. Mol Cell Biochem. 2008;312:61-9 pubmed publisher
    ..Presented data may help to elucidate the role of protein kinase CK2 and Svf1 in the regulation of cell survival pathways. ..
  5. Utley R, Lacoste N, Jobin Robitaille O, Allard S, Cote J. Regulation of NuA4 histone acetyltransferase activity in transcription and DNA repair by phosphorylation of histone H4. Mol Cell Biol. 2005;25:8179-90 pubmed
    ..These findings demonstrate that H4 phospho-serine 1 regulates chromatin acetylation by the NuA4 complex and that this process is important for normal gene expression and DNA repair. ..
  6. Siepe D, Jentsch S. Prolyl isomerase Pin1 acts as a switch to control the degree of substrate ubiquitylation. Nat Cell Biol. 2009;11:967-72 pubmed publisher
    ..This suggests that the Pin1 activity is often translated into a fate-determining ubiquitylation switch, and that Pin1 may affect the degree of substrate ubiquitylation in other pathways as well. ..
  7. Pérez Fernández J, Roman A, De Las Rivas J, Bustelo X, Dosil M. The 90S preribosome is a multimodular structure that is assembled through a hierarchical mechanism. Mol Cell Biol. 2007;27:5414-29 pubmed
    ..Together, our data identify the mechanism of assembly of the 90S preribosome and offer novel information about its internal architecture. ..
  8. Ackermann K, Waxmann A, Glover C, Pyerin W. Genes targeted by protein kinase CK2: a genome-wide expression array analysis in yeast. Mol Cell Biochem. 2001;227:59-66 pubmed
    ..subunit genes in Saccharomyces cerevisiae (CK2alpha isoform genes: CKA1 and CKA2; CK2beta isoform genes: CKB1 and CKB2) was examined using genome-wide expression array analysis (oligonucleotide array chips)...
  9. Farley A, Powell D, Weaver C, Jennings J, Link A. Assessing the components of the eIF3 complex and their phosphorylation status. J Proteome Res. 2011;10:1481-94 pubmed publisher
    ..Replacement of these three Nip1 serines with alanines causes a slow growth phenotype. ..

More Information


  1. Gerus M, Bonnart C, Caizergues Ferrer M, Henry Y, Henras A. Evolutionarily conserved function of RRP36 in early cleavages of the pre-rRNA and production of the 40S ribosomal subunit. Mol Cell Biol. 2010;30:1130-44 pubmed publisher
    ..In parallel, we studied the human orthologue of Rrp36p in HeLa cells, and we show that the function of this protein in early cleavages of the pre-rRNA has been conserved through evolution in eukaryotes. ..
  2. Johnson A, Zaman M, Veljanoski F, Phrakaysone A, Li S, O Doherty P, et al. Unravelling the role of protein kinase CK2 in metal toxicity using gene deletion mutants. Metallomics. 2017;9:301-308 pubmed publisher a pronounced resistant phenotype against Zn2+ and Al3+, whilst the deletion of CKB1 or CKB2 results in tolerance to Cr6+ and As3+...
  3. Hewawasam G, Mattingly M, Venkatesh S, Zhang Y, Florens L, Workman J, et al. Phosphorylation by casein kinase 2 facilitates Psh1 protein-assisted degradation of Cse4 protein. J Biol Chem. 2014;289:29297-309 pubmed publisher
    ..Collectively our data suggest that phosphorylation is crucial in Psh1-assisted control of Cse4 levels and that the Psh1-Cse4 association itself functions to prevent Cse4 misincorporation. ..
  4. Bergkessel M, Whitworth G, Guthrie C. Diverse environmental stresses elicit distinct responses at the level of pre-mRNA processing in yeast. RNA. 2011;17:1461-78 pubmed publisher
    ..In total, our data suggest that complex post-transcriptional programs cooperate to fine-tune expression of intron-containing transcripts in budding yeast. ..
  5. Upton T, Wiltshire S, Francesconi S, Eisenberg S. ABF1 Ser-720 is a predominant phosphorylation site for casein kinase II of Saccharomyces cerevisiae. J Biol Chem. 1995;270:16153-9 pubmed
    ..Thus, phosphorylation of ABF1 by yeast CKII may prove to be a useful system for studying targeting mechanisms of CKII to a physiological substrate. ..
  6. Barz T, Ackermann K, Pyerin W. Perturbation of protein kinase CK2 uncouples executive part of phosphate maintenance pathway from cyclin-CDK control. FEBS Lett. 2003;537:210-4 pubmed
    ..Thus, perturbation of CK2 uncouples the executive part of the PHO pathway from its cyclin-CDK control complex. ..
  7. Cho B, Lee P, Hahn J. CK2-dependent inhibitory phosphorylation is relieved by Ppt1 phosphatase for the ethanol stress-specific activation of Hsf1 in Saccharomyces cerevisiae. Mol Microbiol. 2014;93:306-16 pubmed publisher
    ..Therefore, regulation of the Hsf1 S608 phosphorylation status by reciprocal actions of CK2 and Ppt1 might play an important role to determine Hsf1 sensitivity towards ethanol stress. ..
  8. Liu H, Wang H, Teng M, Li X. The multiple nucleotide-divalent cation binding modes of Saccharomyces cerevisiae CK2? indicate a possible co-substrate hydrolysis product (ADP/GDP) release pathway. Acta Crystallogr D Biol Crystallogr. 2014;70:501-13 pubmed publisher
    ..It is found that the insertion region contributes to maintaining the constitutively active conformation of the scCK2? catalytic site, but does not participate in interaction with the regulatory subunits. ..
  9. Tripodi F, Nicastro R, Busnelli S, Cirulli C, Maffioli E, Tedeschi G, et al. Protein kinase CK2 holoenzyme promotes start-specific transcription in Saccharomyces cerevisiae. Eukaryot Cell. 2013;12:1271-80 pubmed publisher
    ..Here we investigate the function of the CK2 holoenzyme in Start-specific transcription. The ckb1? ckb2? mutant strain, bearing deletions of both genes encoding CK2 regulatory subunits, shows a delay of S-phase entrance ..
  10. Janeczko M, Masłyk M, Szyszka R, Baier A. Interactions between subunits of protein kinase CK2 and their protein substrates influences its sensitivity to specific inhibitors. Mol Cell Biochem. 2011;356:121-6 pubmed publisher
    ..Conceivably, a given inhibitor concentration can inhibit only selected CK2-mediated processes in the cell. ..
  11. Toczyski D, Galgoczy D, Hartwell L. CDC5 and CKII control adaptation to the yeast DNA damage checkpoint. Cell. 1997;90:1097-106 pubmed that one mutation resides in CDC5, which encodes a polo-like kinase, whereas a second, less penetrant, adaptation-defective mutant is affected at the CKB2 locus, which encodes a nonessential specificity subunit of casein kinase II.
  12. Ghavidel A, Schultz M. TATA binding protein-associated CK2 transduces DNA damage signals to the RNA polymerase III transcriptional machinery. Cell. 2001;106:575-84 pubmed
    ..Therefore, CK2 is the terminal effector in a signaling pathway that represses pol III transcription when genome integrity is compromised. ..
  13. Schaerer Brodbeck C, Riezman H. Genetic and biochemical interactions between the Arp2/3 complex, Cmd1p, casein kinase II, and Tub4p in yeast. FEMS Yeast Res. 2003;4:37-49 pubmed
  14. Bhat W, Boutin G, Rufiange A, Nourani A. Casein kinase 2 associates with the yeast chromatin reassembly factor Spt2/Sin1 to regulate its function in the repression of spurious transcription. Mol Cell Biol. 2013;33:4198-211 pubmed publisher
    ..Taken together, our data suggest that CK2 regulates the function of Spt2 by modulating its interaction with chromatin and the histone chaperone Spt6. ..
  15. Kurat C, Lambert J, van Dyk D, Tsui K, van Bakel H, Kaluarachchi S, et al. Restriction of histone gene transcription to S phase by phosphorylation of a chromatin boundary protein. Genes Dev. 2011;25:2489-501 pubmed publisher
    ..Our study identified the chromatin boundary protein Yta7 as a key regulator that links S-phase kinases with RNAPII function at cell cycle-regulated histone gene promoters. ..
  16. Hsieh L, Su W, Han G, Carman G. Phosphorylation of Yeast Pah1 Phosphatidate Phosphatase by Casein Kinase II Regulates Its Function in Lipid Metabolism. J Biol Chem. 2016;291:9974-90 pubmed publisher
  17. Sawa C, Nedea E, Krogan N, Wada T, Handa H, Greenblatt J, et al. Bromodomain factor 1 (Bdf1) is phosphorylated by protein kinase CK2. Mol Cell Biol. 2004;24:4734-42 pubmed
    ..A similar mechanism may be responsible for phosphorylation of the C-terminal region of mammalian TAF1. These findings suggest that CK2 phosphorylation of Bdf1 may regulate RNA polymerase II transcription and/or chromatin structure. ..
  18. Berkey C, Carlson M. A specific catalytic subunit isoform of protein kinase CK2 is required for phosphorylation of the repressor Nrg1 in Saccharomyces cerevisiae. Curr Genet. 2006;50:1-10 pubmed
    ..This identification of a protein whose phosphorylation requires a specific CK2 catalytic subunit isoform supports the view that the two isoforms exhibit functional specificity in vivo. ..
  19. Kubinski K, Domanska K, Sajnaga E, Mazur E, Zieliński R, Szyszka R. Yeast holoenzyme of protein kinase CK2 requires both beta and beta' regulatory subunits for its activity. Mol Cell Biochem. 2007;295:229-36 pubmed
    ..strains containing single deletion of the beta or beta' regulatory subunits with vectors carrying lacking CKB1 or CKB2 genes. CK2 holoenzyme activity was restored only in cases when both of them were present in the cell...
  20. Bidwai A, Reed J, Glover C. Casein kinase II of Saccharomyces cerevisiae contains two distinct regulatory subunits, beta and beta'. Arch Biochem Biophys. 1994;309:348-55 pubmed
    ..This represents the first demonstration of beta subunit heterogeneity in purified CKII from any species. ..
  21. Kim M, Hahn J. Role of CK2-dependent phosphorylation of Ifh1 and Crf1 in transcriptional regulation of ribosomal protein genes in Saccharomyces cerevisiae. Biochim Biophys Acta. 2016;1859:1004-13 pubmed publisher
    ..Taken together, these findings suggest the mechanisms by which CK2-dependent recruitment of Ifh1 and Crf1 at the RP gene promoters governs the transcription of RP genes. ..
  22. Rudra D, Mallick J, Zhao Y, Warner J. Potential interface between ribosomal protein production and pre-rRNA processing. Mol Cell Biol. 2007;27:4815-24 pubmed
    ..We propose a model in which the CURI complex is a key mediator between the two parallel pathways necessary for ribosome synthesis: the transcription and processing of pre-rRNA and the transcription of ribosomal protein genes. ..
  23. Pickin K, Ezenwajiaku N, Overcash H, Sethi M, Knecht M, Paumi C. Suppression of Ycf1p function by Cka1p-dependent phosphorylation is attenuated in response to salt stress. FEMS Yeast Res. 2010;10:839-57 pubmed publisher
    ..In conclusion, our work demonstrates a novel biochemical role for Cka1p regulation of Ycf1p function in the cellular response of yeast to salt stress...
  24. Barz T, Ackermann K, Pyerin W. Control of methionine biosynthesis genes by protein kinase CK2-mediated phosphorylation of Cdc34. Cell Mol Life Sci. 2006;63:2183-90 pubmed
  25. Schmidt O, Harbauer A, Rao S, Eyrich B, Zahedi R, Stojanovski D, et al. Regulation of mitochondrial protein import by cytosolic kinases. Cell. 2011;144:227-39 pubmed publisher
    ..We conclude that cytosolic kinases exert stimulatory and inhibitory effects on biogenesis and function of the TOM complex and thus regulate protein import into mitochondria. ..
  26. Barberis M, Pagano M, Gioia L, Marin O, Vanoni M, Pinna L, et al. CK2 regulates in vitro the activity of the yeast cyclin-dependent kinase inhibitor Sic1. Biochem Biophys Res Commun. 2005;336:1040-8 pubmed
    ..Taken together, these data disclose the possibility that CK2 plays a role in the regulation of Sic1 activity. ..
  27. Albert B, Knight B, Merwin J, Martin V, Ottoz D, Gloor Y, et al. A Molecular Titration System Coordinates Ribosomal Protein Gene Transcription with Ribosomal RNA Synthesis. Mol Cell. 2016;64:720-733 pubmed publisher
    ..We present evidence that RNA polymerase I activity inhibits the ability of Utp22 to titrate Ifh1 from RPG promoters and propose that a dynamic Ifh1-Utp22 interaction fine-tunes RPG expression to coordinate RPG and rRNA transcription. ..