Gene Symbol: CIT1
Description: citrate (Si)-synthase CIT1
Alias: LYS6, citrate (Si)-synthase CIT1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Jia Y, Becam A, Herbert C. The CIT3 gene of Saccharomyces cerevisiae encodes a second mitochondrial isoform of citrate synthase. Mol Microbiol. 1997;24:53-9 pubmed
    ..The CIT3 gene seems to be regulated in the same way as CIT1, which encodes the mitochondrial isoform of citrate synthase...
  2. Kim K, Rosenkrantz M, Guarente L. Saccharomyces cerevisiae contains two functional citrate synthase genes. Mol Cell Biol. 1986;6:1936-42 pubmed
    ..Suissa, K. Suda, and G. Schatz, EMBO J. 3:1773-1781, 1984) and is referred to here as CIT1. We report here the isolation, by an immunological method, of a second nuclear gene encoding citrate synthase (CIT2)..
  3. Lin A, Hakala K, Weintraub S, McAlister Henn L. Suppression of metabolic defects of yeast isocitrate dehydrogenase and aconitase mutants by loss of citrate synthase. Arch Biochem Biophys. 2008;474:205-12 pubmed publisher
    ..These shared properties of idhDelta and aco1Delta strains were eliminated or moderated by co-disruption of the CIT1 gene encoding mitochondrial citrate synthase...
  4. Farooq M, Pracheil T, Dong Z, Xiao F, Liu Z. Mitochondrial DNA instability in cells lacking aconitase correlates with iron citrate toxicity. Oxid Med Cell Longev. 2013;2013:493536 pubmed publisher
    ..We found that loss of Sod1, but not Sod2, prevents mtDNA loss in aco1? mutant cells. We propose that mtDNA loss in aco1? mutant cells is caused by the activation of the RTG pathway and subsequent iron citrate accumulation and toxicity. ..
  5. Przybyla Zawislak B, Gadde D, Ducharme K, McCammon M. Genetic and biochemical interactions involving tricarboxylic acid cycle (TCA) function using a collection of mutants defective in all TCA cycle genes. Genetics. 1999;152:153-66 pubmed
    ..grow poorly on glycerol, but growth can be enhanced by extragenic mutations, termed glycerol suppressors, in the CIT1 gene encoding the TCA cycle citrate synthase and in other genes of oxidative metabolism...
  6. Bender T, Leidhold C, Ruppert T, Franken S, Voos W. The role of protein quality control in mitochondrial protein homeostasis under oxidative stress. Proteomics. 2010;10:1426-43 pubmed publisher
    ..As Pim1/LON expression was induced significantly under ROS treatment, we propose that this protease system performs a crucial protective function under oxidative stress conditions. ..
  7. Vélot C, Srere P. Reversible transdominant inhibition of a metabolic pathway. In vivo evidence of interaction between two sequential tricarboxylic acid cycle enzymes in yeast. J Biol Chem. 2000;275:12926-33 pubmed
  8. Dallabona C, Marsano R, Arzuffi P, Ghezzi D, Mancini P, Zeviani M, et al. Sym1, the yeast ortholog of the MPV17 human disease protein, is a stress-induced bioenergetic and morphogenetic mitochondrial modulator. Hum Mol Genet. 2010;19:1098-107 pubmed publisher
  9. Brandina I, Graham J, Lemaitre Guillier C, Entelis N, Krasheninnikov I, Sweetlove L, et al. Enolase takes part in a macromolecular complex associated to mitochondria in yeast. Biochim Biophys Acta. 2006;1757:1217-28 pubmed
    ..This suggests an unsuspected novel function for this complex in tRNA mitochondrial import. ..

More Information


  1. Lee Y, Jang J, Kim K, Maeng P. TCA cycle-independent acetate metabolism via the glyoxylate cycle in Saccharomyces cerevisiae. Yeast. 2011;28:153-66 pubmed publisher
    In Saccharomyces cerevisiae, the accepted theory is that due to TCA cycle dysfunction, the ?cit1 mutant lacking the mitochondrial enzyme citrate synthase (Cit1) cannot grow on acetate, regardless of the presence of the peroxisomal ..
  2. Lee Y, Hoe K, Maeng P. Yeast cells lacking the CIT1-encoded mitochondrial citrate synthase are hypersusceptible to heat- or aging-induced apoptosis. Mol Biol Cell. 2007;18:3556-67 pubmed
    ..cerevisiae, the initial reaction of the tricarboxylic acid cycle is catalyzed by the mitochondrial citrate synthase Cit1. The function of Cit1 has previously been studied mainly in terms of acetate utilization and metabolon construction...
  3. Graybill E, Rouhier M, Kirby C, Hawes J. Functional comparison of citrate synthase isoforms from S. cerevisiae. Arch Biochem Biophys. 2007;465:26-37 pubmed
    ..gene has been identified as a dual specificity mitochondrial citrate and methylcitrate synthase and that of the CIT1 gene as a specific citrate synthase...
  4. Lee J, Cho S, Lee H, Lee C, Bae K, Maeng P. Identification of a cryptic N-terminal signal in Saccharomyces cerevisiae peroxisomal citrate synthase that functions in both peroxisomal and mitochondrial targeting. J Biochem. 2000;128:1059-72 pubmed
    ..Both fusions, Cit1::Cit2 and Cit2::Cit1, complemented the glutamate auxotrophy caused by the double-disruption of the CIT1 and CIT2 ..
  5. McAlister Henn L, Small W. Molecular genetics of yeast TCA cycle isozymes. Prog Nucleic Acid Res Mol Biol. 1997;57:317-39 pubmed
  6. Small W, Brodeur R, Sandor A, Fedorova N, Li G, Butow R, et al. Enzymatic and metabolic studies on retrograde regulation mutants of yeast. Biochemistry. 1995;34:5569-76 pubmed
  7. Lee J, Lee Y, Lee C, Maeng P. Mutational and functional analysis of the cryptic N-terminal targeting signal for both mitochondria and peroxisomes in yeast peroxisomal citrate synthase Cit2p. J Biochem. 2006;140:121-33 pubmed
    ..It is thus suggested that the potential of the N-terminal segment as an ambidextrous targeting signal can be unmasked by deletion of PTS1. ..
  8. Grandier Vazeille X, Bathany K, Chaignepain S, Camougrand N, Manon S, Schmitter J. Yeast mitochondrial dehydrogenases are associated in a supramolecular complex. Biochemistry. 2001;40:9758-69 pubmed
    ..The association of these proteins is discussed in terms of NADH-channeling. ..
  9. Vélot C, Lebreton S, Morgunov I, Usher K, Srere P. Metabolic effects of mislocalized mitochondrial and peroxisomal citrate synthases in yeast Saccharomyces cerevisiae. Biochemistry. 1999;38:16195-204 pubmed
    Genes CIT1 and CIT2 from Saccharomyces cerevisiae encode mitochondrial and peroxisomal citrate synthases involved in the Krebs tricarboxylic acid (TCA) cycle and glyoxylate pathway, respectively...
  10. Beaufour M, Godin F, Vallee B, Cadene M, Bénédetti H. Interaction proteomics suggests a new role for the Tfs1 protein in yeast. J Proteome Res. 2012;11:3211-8 pubmed publisher
    ..Integration of these results with known functional partners of Tfs1p shows that two subnetworks meet through the Tfs1p node, suggesting that it may act as a bridge between cell signaling and intermediate metabolism in yeast. ..
  11. Belenky P, Camacho D, Collins J. Fungicidal drugs induce a common oxidative-damage cellular death pathway. Cell Rep. 2013;3:350-8 pubmed publisher
    ..Lastly, we demonstrate that DNA damage plays a critical role in antifungal-induced cellular death and that blocking DNA-repair mechanisms potentiates fungicidal activity. ..