CIN8

Summary

Gene Symbol: CIN8
Description: kinesin motor protein CIN8
Alias: KSL2, SDS15, kinesin motor protein CIN8
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Hoyt M, He L, Totis L, Saunders W. Loss of function of Saccharomyces cerevisiae kinesin-related CIN8 and KIP1 is suppressed by KAR3 motor domain mutations. Genetics. 1993;135:35-44 pubmed
    The kinesin-related products of the CIN8 and KIP1 genes of Saccharomyces cerevisiae redundantly perform an essential function in mitosis...
  2. Basmaji F, Martin Yken H, Durand F, Dagkessamanskaia A, Pichereaux C, Rossignol M, et al. The 'interactome' of the Knr4/Smi1, a protein implicated in coordinating cell wall synthesis with bud emergence in Saccharomyces cerevisiae. Mol Genet Genomics. 2006;275:217-30 pubmed
    ..The second group comprised four proteins (Bud6, Act1, Cin8 and Jnm1) implicated in the establishment of cell polarity and bud integrity during mitosis...
  3. Hoyt M, He L, Loo K, Saunders W. Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly. J Cell Biol. 1992;118:109-20 pubmed
    Two Saccharomyces cerevisiae genes, CIN8 and KIP1 (a.k.a. CIN9), were identified by their requirement for normal chromosome segregation...
  4. Roostalu J, Hentrich C, Bieling P, Telley I, Schiebel E, Surrey T. Directional switching of the kinesin Cin8 through motor coupling. Science. 2011;332:94-9 pubmed publisher
    ..Kinesin-5 motors are considered to be plus-end-directed. However, here we found that purified kinesin-5 Cin8 from budding yeast could behave as a bidirectional kinesin...
  5. Chiroli E, Rancati G, Catusi I, Lucchini G, Piatti S. Cdc14 inhibition by the spindle assembly checkpoint prevents unscheduled centrosome separation in budding yeast. Mol Biol Cell. 2009;20:2626-37 pubmed publisher
    ..We propose that, besides inhibiting sister chromatid separation, the SAC preserves the accurate transmission of chromosomes also by preventing SPBs to migrate far apart until the conditions to assemble a bipolar spindle are satisfied. ..
  6. Fridman V, Gerson Gurwitz A, Movshovich N, Kupiec M, Gheber L. Midzone organization restricts interpolar microtubule plus-end dynamics during spindle elongation. EMBO Rep. 2009;10:387-93 pubmed publisher
    ..We also observed that deletion of Cin8, a microtubule-crosslinking kinesin-5 motor protein, reduced the recovery rate in anaphase spindles, indicating ..
  7. Movshovich N, Fridman V, Gerson Gurwitz A, Shumacher I, Gertsberg I, Fich A, et al. Slk19-dependent mid-anaphase pause in kinesin-5-mutated cells. J Cell Sci. 2008;121:2529-39 pubmed publisher
    ..We examined spindle elongation in anaphase in Saccharomyces cerevisiae cells mutated for the kinesin-5 motor proteins Cin8 and Kip1...
  8. Yin H, You L, Pasqualone D, Kopski K, Huffaker T. Stu1p is physically associated with beta-tubulin and is required for structural integrity of the mitotic spindle. Mol Biol Cell. 2002;13:1881-92 pubmed
    ..Taken together, these findings suggest that Stu1p binds to interpolar microtubules of the mitotic spindle and plays an essential role in their ability to provide an outward force on the spindle poles. ..
  9. Saunders W, Lengyel V, Hoyt M. Mitotic spindle function in Saccharomyces cerevisiae requires a balance between different types of kinesin-related motors. Mol Biol Cell. 1997;8:1025-33 pubmed
    ..Kar3p, may act antagonistically to Cin8p and Kip1p since loss of Kar3p partially suppresses the spindle collapse in cin8 kip1 mutants...

More Information

Publications59

  1. Gerson Gurwitz A, Movshovich N, Avunie R, Fridman V, Moyal K, Katz B, et al. Mid-anaphase arrest in S. cerevisiae cells eliminated for the function of Cin8 and dynein. Cell Mol Life Sci. 2009;66:301-13 pubmed publisher
    ..anaphase spindle elongation is accomplished by the overlapping function of dynein and the kinesin-5 motor proteins, Cin8 and Kip1...
  2. Roof D, Meluh P, Rose M. Kinesin-related proteins required for assembly of the mitotic spindle. J Cell Biol. 1992;118:95-108 pubmed
    ..Using a genetic screen for mutations that make KIP1 essential, we identified another gene, KSL2, which proved to be another kinesin-related gene, CIN8...
  3. Korolyev E, Steinberg Neifach O, Eshel D. Mutations in the yeast kinesin-like Cin8p are alleviated by osmotic support. FEMS Microbiol Lett. 2005;244:379-83 pubmed
    ..We report that cin8 mutants are sensitive to the cell wall disrupting agents calcofluor white and SDS...
  4. Jones M, Bachant J, Castillo A, Giddings T, Winey M. Yeast Dam1p is required to maintain spindle integrity during mitosis and interacts with the Mps1p kinase. Mol Biol Cell. 1999;10:2377-91 pubmed
    ..Consistent with this phenotype, DAM1 displays genetic interactions with STU1, CIN8, and KAR3, genes encoding proteins involved in spindle function...
  5. Liu H, Liang F, Jin F, Wang Y. The coordination of centromere replication, spindle formation, and kinetochore-microtubule interaction in budding yeast. PLoS Genet. 2008;4:e1000262 pubmed publisher
    ..Overproduction of microtubule-associated protein Ase1 or Cin8 also induces spindle elongation in HU-arrested cells...
  6. Zeng X, Kahana J, Silver P, Morphew M, McIntosh J, Fitch I, et al. Slk19p is a centromere protein that functions to stabilize mitotic spindles. J Cell Biol. 1999;146:415-25 pubmed
    ..Thus, these proteins seem to play overlapping roles in stabilizing spindle structure while acting from opposite ends of the microtubules. ..
  7. Straight A, Sedat J, Murray A. Time-lapse microscopy reveals unique roles for kinesins during anaphase in budding yeast. J Cell Biol. 1998;143:687-94 pubmed
    ..The kip3Delta and kip1Delta mutants affect the duration of anaphase, but cin8Delta does not. ..
  8. Hildebrandt E, Gheber L, Kingsbury T, Hoyt M. Homotetrameric form of Cin8p, a Saccharomyces cerevisiae kinesin-5 motor, is essential for its in vivo function. J Biol Chem. 2006;281:26004-13 pubmed
    ..These findings suggest that binding of microtubules is not sufficient for the functionality of Cin8p and that microtubule cross-linking by the tetrameric complex is essential for Cin8p mitotic functions. ..
  9. Gerson Gurwitz A, Thiede C, Movshovich N, Fridman V, Podolskaya M, Danieli T, et al. Directionality of individual kinesin-5 Cin8 motors is modulated by loop 8, ionic strength and microtubule geometry. EMBO J. 2011;30:4942-54 pubmed publisher
    ..The Saccharomyces cerevisiae kinesin-5 Cin8 was found, surprisingly, to switch directionality...
  10. Saunders W, Koshland D, Eshel D, Gibbons I, Hoyt M. Saccharomyces cerevisiae kinesin- and dynein-related proteins required for anaphase chromosome segregation. J Cell Biol. 1995;128:617-24 pubmed
    ..for function between different types of microtubule-based motor proteins was also indicated by the observation that cin8 dyn1 double-deletion mutants are inviable...
  11. Hardwick K, Li R, Mistrot C, Chen R, Dann P, Rudner A, et al. Lesions in many different spindle components activate the spindle checkpoint in the budding yeast Saccharomyces cerevisiae. Genetics. 1999;152:509-18 pubmed
    ..In contrast, the cell cycle arrest caused by mutations that induce DNA damage (cdc13), inactivate the cyclin proteolysis machinery (cdc16 and cdc23), or arrest cells in anaphase (cdc15) is independent of the spindle checkpoint. ..
  12. Kotwaliwale C, Frei S, Stern B, Biggins S. A pathway containing the Ipl1/aurora protein kinase and the spindle midzone protein Ase1 regulates yeast spindle assembly. Dev Cell. 2007;13:433-45 pubmed
    ..revealed that it is required for centrosome-mediated spindle assembly in the absence of the BimC motor protein Cin8. In addition, we found that the Ase1 spindle midzone-associated protein is required for bipolar spindle assembly...
  13. Woodruff J, Drubin D, Barnes G. Mitotic spindle disassembly occurs via distinct subprocesses driven by the anaphase-promoting complex, Aurora B kinase, and kinesin-8. J Cell Biol. 2010;191:795-808 pubmed publisher
    ..Combinatorial inhibition of these subprocesses yielded cells with hyperstable spindle remnants and dramatic defects in cell cycle progression, establishing that rapid spindle disassembly is crucial for cell proliferation. ..
  14. Chee M, Haase S. B-cyclin/CDKs regulate mitotic spindle assembly by phosphorylating kinesins-5 in budding yeast. PLoS Genet. 2010;6:e1000935 pubmed publisher
    ..Clb/Cdc28) complexes promote spindle pole separation by inhibiting the degradation of the kinesins-5 Kip1 and Cin8 by the anaphase-promoting complex (APC(Cdh1))...
  15. Gardner M, Bouck D, Paliulis L, Meehl J, O TOOLE E, Haase J, et al. Chromosome congression by Kinesin-5 motor-mediated disassembly of longer kinetochore microtubules. Cell. 2008;135:894-906 pubmed publisher
    ..In conclusion, we find that length-dependent control of net kMT assembly by kinesin-5 motors yields a simple and stable self-organizing mechanism for chromosome congression...
  16. He X, Rines D, Espelin C, Sorger P. Molecular analysis of kinetochore-microtubule attachment in budding yeast. Cell. 2001;106:195-206 pubmed
    ..The proteins include both molecular motors and microtubule associated proteins (MAPs), implying that motors and MAPs function together in binding chromosomes to spindle microtubules. ..
  17. Saunders W, Hoyt M. Kinesin-related proteins required for structural integrity of the mitotic spindle. Cell. 1992;70:451-8 pubmed
    ..Our findings suggest that the structure of the preanaphase bipolar spindle is maintained by counteracting forces produced by kinesin-related proteins. ..
  18. Cheeseman I, Enquist Newman M, MULLER REICHERT T, Drubin D, Barnes G. Mitotic spindle integrity and kinetochore function linked by the Duo1p/Dam1p complex. J Cell Biol. 2001;152:197-212 pubmed
    ..In addition, Duo1p and Dam1p localize to kinetochores in chromosome spreads, suggesting that this complex may serve as a link between the kinetochore and the mitotic spindle. ..
  19. Krishnan V, Nirantar S, Crasta K, Cheng A, Surana U. DNA replication checkpoint prevents precocious chromosome segregation by regulating spindle behavior. Mol Cell. 2004;16:687-700 pubmed
    ..Instead, the checkpoint deficiency leads to deregulation of microtubule-associated proteins Cin8 and Stu2, which, in the absence of both chromosome cohesion and bipolar attachment of kinetochores to microtubules, ..
  20. Khmelinskii A, Roostalu J, Roque H, Antony C, Schiebel E. Phosphorylation-dependent protein interactions at the spindle midzone mediate cell cycle regulation of spindle elongation. Dev Cell. 2009;17:244-56 pubmed publisher
    ..Here we demonstrate that phosphorylation of the midzone organizer Ase1 controls localization and function of Cin8, a kinesin-5 that slides iMTs relative to each other...
  21. Geiser J, Schott E, Kingsbury T, Cole N, Totis L, Bhattacharyya G, et al. Saccharomyces cerevisiae genes required in the absence of the CIN8-encoded spindle motor act in functionally diverse mitotic pathways. Mol Biol Cell. 1997;8:1035-50 pubmed
    ..These findings illustrate the complex cellular interactions exhibited by Cin8p, a member of a conserved spindle motor family. ..
  22. Laman H, Balderes D, Shore D. Disturbance of normal cell cycle progression enhances the establishment of transcriptional silencing in Saccharomyces cerevisiae. Mol Cell Biol. 1995;15:3608-17 pubmed
    ..SDS12 is identical to RNR1, which encodes a subunit of ribonucleotide reductase. SDS15 is identical to CIN8, whose product is required for spindle formation...
  23. Fallesen T, Roostalu J, Duellberg C, Pruessner G, Surrey T. Ensembles of Bidirectional Kinesin Cin8 Produce Additive Forces in Both Directions of Movement. Biophys J. 2017;113:2055-2067 pubmed publisher
    ..Using force spectroscopy, we have measured here the forces that ensembles of purified budding yeast kinesin-5 Cin8 produce in microtubule gliding assays in both plus- and minus-end direction...
  24. Samel A, Cuomo A, Bonaldi T, Ehrenhofer Murray A. Methylation of CenH3 arginine 37 regulates kinetochore integrity and chromosome segregation. Proc Natl Acad Sci U S A. 2012;109:9029-34 pubmed publisher
    ..Altogether, our data identify a unique regulatory principle on centromeric chromatin by posttranslational modification of the amino terminus of CenH3. ..
  25. Düselder A, Fridman V, Thiede C, Wiesbaum A, Goldstein A, Klopfenstein D, et al. Deletion of the Tail Domain of the Kinesin-5 Cin8 Affects Its Directionality. J Biol Chem. 2015;290:16841-50 pubmed publisher
    ..The budding yeast kinesin-5 Cin8 was shown to switch from fast minus-end- to slow plus-end-directed motility upon binding between antiparallel MTs...
  26. Schott E, Hoyt M. Dominant alleles of Saccharomyces cerevisiae CDC20 reveal its role in promoting anaphase. Genetics. 1998;148:599-610 pubmed
    ..Cdc20p may promote the APC-dependent proteolytic degradation of Pds1p and other factors that act to inhibit cell cycle progression through mitosis. ..
  27. Lee L, Klee S, Evangelista M, Boone C, Pellman D. Control of mitotic spindle position by the Saccharomyces cerevisiae formin Bni1p. J Cell Biol. 1999;144:947-61 pubmed
    ..Additionally, we present evidence that other bipolar bud site determinants together with cortical actin are also required for spindle orientation. ..
  28. Steinberg Neifach O, Eshel D. Simultaneous expression of both MAT loci in haploid cells suppresses mutations in yeast microtubule motor genes. Mol Gen Genet. 2000;264:300-5 pubmed
    ..Cells deleted for DYN1 (the gene encoding the dynein heavy chain) and carrying the temperature-sensitive allele cin8-3 cannot grow at temperatures above 35 degrees C...
  29. de Gramont A, Barbour L, Ross K, COHEN FIX O. The spindle midzone microtubule-associated proteins Ase1p and Cin8p affect the number and orientation of astral microtubules in Saccharomyces cerevisiae. Cell Cycle. 2007;6:1231-41 pubmed
    ..We show here that deletion of ASE1 or CIN8 causes a defect in SPB separation and leads to an abnormal number of astral microtubules and a change in their ..
  30. Anderson V, Prudden J, Prochnik S, Giddings T, Hardwick K. Novel sfi1 alleles uncover additional functions for Sfi1p in bipolar spindle assembly and function. Mol Biol Cell. 2007;18:2047-56 pubmed
    ..3 microm apart. Importantly, these SPBs have completed duplication, but they are not separated, suggesting a possible defect in splitting of the bridge. We discuss possible roles for Sfi1p in this step in bipolar spindle assembly. ..
  31. Hoyt M, Macke J, Roberts B, Geiser J. Saccharomyces cerevisiae PAC2 functions with CIN1, 2 and 4 in a pathway leading to normal microtubule stability. Genetics. 1997;146:849-57 pubmed
    ..Our findings suggest that the guanine nucleotide-binding Cin4p interacts with Cin1p and regulates its tubulin folding activity. ..
  32. Su X, Arellano Santoyo H, Portran D, Gaillard J, Vantard M, Thery M, et al. Microtubule-sliding activity of a kinesin-8 promotes spindle assembly and spindle-length control. Nat Cell Biol. 2013;15:948-57 pubmed publisher
    ..In conjunction with Cin8, a kinesin-5 family member, the sliding activity of Kip3 promotes bipolar spindle assembly and the maintenance of ..
  33. He J, Chao W, Zhang Z, Yang J, Cronin N, Barford D. Insights into degron recognition by APC/C coactivators from the structure of an Acm1-Cdh1 complex. Mol Cell. 2013;50:649-60 pubmed publisher
    ..We provide a structural rationalization for D box and KEN box recognition by coactivators and demonstrate that many noncanonical APC/C degrons bind APC/C coactivators at the D box coreceptor...
  34. Manfrini N, Gobbini E, Baldo V, Trovesi C, Lucchini G, Longhese M. G(1)/S and G(2)/M cyclin-dependent kinase activities commit cells to death in the absence of the S-phase checkpoint. Mol Cell Biol. 2012;32:4971-85 pubmed publisher
    ..Moreover, these findings suggest that the essential function of Mec1 and Rad53 is not necessarily separated from the function of these kinases in supporting DNA synthesis under stress conditions. ..
  35. Bell K, Cha H, Sindelar C, Cochran J. The yeast kinesin-5 Cin8 interacts with the microtubule in a noncanonical manner. J Biol Chem. 2017;292:14680-14694 pubmed publisher
    ..Unlike most other kinesins, Cin8, a kinesin-5 motor in Saccharomyces cerevisiae, can move bidirectionally along microtubules, switching ..
  36. Shohat Tal A, Eshel D. Cell cycle regulators interact with pathways that modulate microtubule stability in Saccharomyces cerevisiae. Eukaryot Cell. 2011;10:1705-13 pubmed publisher
    ..Our results introduce novel roles in microtubule stability for genes whose participation in the process may be masked under normal conditions yet nonetheless acquire a dominant role when microtubule stability is compromised. ..
  37. Roccuzzo M, Visintin C, Tili F, Visintin R. FEAR-mediated activation of Cdc14 is the limiting step for spindle elongation and anaphase progression. Nat Cell Biol. 2015;17:251-61 pubmed publisher
    ..We further identify the kinesin-5 motor protein Cin8 as a key target of Cdc14...
  38. Makhnevych T, Wong P, Pogoutse O, Vizeacoumar F, Greenblatt J, Emili A, et al. Hsp110 is required for spindle length control. J Cell Biol. 2012;198:623-36 pubmed publisher
    ..in spindle assembly and, more specifically, in modulating the activity of the widely conserved kinesin-5 motor Cin8. The role of Hsp110 Sse1 as a nucleotide exchange factor for the Hsp70 chaperones Ssa1/Ssa2 was found to be ..
  39. Strawn L, True H. Deletion of RNQ1 gene reveals novel functional relationship between divergently transcribed Bik1p/CLIP-170 and Sfi1p in spindle pole body separation. Curr Genet. 2006;50:347-66 pubmed
    ..These results suggest that Bik1p has a previously uncharacterized function in the separation of duplicated SPBs. ..
  40. Xie Y, Varshavsky A. The N-end rule pathway is required for import of histidine in yeast lacking the kinesin-like protein Cin8p. Curr Genet. 1999;36:113-23 pubmed
    ..This feature of sln2 allowed the cloning of SLN2, which was found to be a gene called CIN8, encoding a kinesin-like protein...
  41. Chai C, Teh E, Yeong F. Unrestrained spindle elongation during recovery from spindle checkpoint activation in cdc15-2 cells results in mis-segregation of chromosomes. Mol Biol Cell. 2010;21:2384-98 pubmed publisher
    ..Our data indicate that a slower rate of transition through the cell division cycle can result in an inadequate level of Pds1p accumulation that can compromise recovery from spindle assembly checkpoint activation. ..
  42. Shapira O, Gheber L. Motile properties of the bi-directional kinesin-5 Cin8 are affected by phosphorylation in its motor domain. Sci Rep. 2016;6:25597 pubmed publisher
    The Saccharomyces cerevisiae kinesin-5 Cin8 performs essential mitotic functions in spindle assembly and anaphase B spindle elongation...
  43. Avunie Masala R, Movshovich N, Nissenkorn Y, Gerson Gurwitz A, Fridman V, Kõivomägi M, et al. Phospho-regulation of kinesin-5 during anaphase spindle elongation. J Cell Sci. 2011;124:873-8 pubmed publisher
    The kinesin-5 Saccharomyces cerevisiae homologue Cin8 is shown here to be differentially phosphorylated during late anaphase at Cdk1-specific sites located in its motor domain...
  44. Fraschini R, D Ambrosio C, Venturetti M, Lucchini G, Piatti S. Disappearance of the budding yeast Bub2-Bfa1 complex from the mother-bound spindle pole contributes to mitotic exit. J Cell Biol. 2006;172:335-46 pubmed
    ..Moreover, it correlates with the passage of one spindle pole through the bud neck because it needs septin ring formation and bud neck kinases. ..
  45. Cui H, Ghosh S, Jayaram M. The selfish yeast plasmid uses the nuclear motor Kip1p but not Cin8p for its localization and equal segregation. J Cell Biol. 2009;185:251-64 pubmed publisher
    The 2 micron plasmid of Saccharomyces cerevisiae uses the Kip1 motor, but not the functionally redundant Cin8 motor, for its precise nuclear localization and equal segregation...
  46. Seybold C, Elserafy M, Rüthnick D, Ozboyaci M, Neuner A, Flottmann B, et al. Kar1 binding to Sfi1 C-terminal regions anchors the SPB bridge to the nuclear envelope. J Cell Biol. 2015;209:843-61 pubmed publisher
    ..In our model, Kar1 binding is restricted to Sfi1-CT and Sfi1 C-terminal centrin-binding repeats, and centrin and Kar1 provide cross-links, while Sfi1-CT stabilizes the bridge and ensures timely SPB separation. ..
  47. Crasta K, Lim H, Giddings T, Winey M, Surana U. Inactivation of Cdh1 by synergistic action of Cdk1 and polo kinase is necessary for proper assembly of the mitotic spindle. Nat Cell Biol. 2008;10:665-75 pubmed publisher
    ..In Saccharomyces cerevisiae, SPB separation also requires activated Cdk1 and the plus-end kinesins Cin8 (homologous to vertebrate Eg5) and Kip1. Here we report that polo kinase has a role in the separation of SPBs...
  48. Goldstein A, Siegler N, Goldman D, Judah H, Valk E, Kõivomägi M, et al. Three Cdk1 sites in the kinesin-5 Cin8 catalytic domain coordinate motor localization and activity during anaphase. Cell Mol Life Sci. 2017;74:3395-3412 pubmed publisher
    ..phosphorylation of at least one of the Cdk1 sites in the catalytic domain of the Saccharomyces cerevisiae kinesin-5 Cin8 (S277, T285, S493) regulates its localization to the anaphase spindle...
  49. McKnight K, Liu H, Wang Y. Replicative stress induces intragenic transcription of the ASE1 gene that negatively regulates Ase1 activity. Curr Biol. 2014;24:1101-6 pubmed publisher
    ..Together, our results reveal intragenic transcription as a unique mechanism to downregulate gene functions in response to DNA replication stress. ..
  50. Lee M, Spencer F. Bipolar orientation of chromosomes in Saccharomyces cerevisiae is monitored by Mad1 and Mad2, but not by Mad3. Proc Natl Acad Sci U S A. 2004;101:10655-60 pubmed
    ..Cells without MAD3 were checkpoint-proficient. We conclude that Mad1 and Mad2 are required to detect bipolar orientation and/or tension at kinetochores, whereas Mad3 is not. ..