Gene Symbol: CIN2
Description: GTPase-activating protein CIN2
Alias: GTPase-activating protein CIN2
Species: Saccharomyces cerevisiae S288c
Berlin V, Styles C, Fink G. BIK1, a protein required for microtubule function during mating and mitosis in Saccharomyces cerevisiae, colocalizes with tubulin. J Cell Biol. 1990;111:2573-86 pubmed
..Based on these results, we propose that BIK1 is required stoichiometrically for the formation or stabilization of microtubules during mitosis and for spindle pole body fusion during conjugation. ..
Nithianantham S, Le S, Seto E, Jia W, Leary J, Corbett K, et al
. Tubulin cofactors and Arl2 are cage-like chaperones that regulate the soluble Î±Î²-tubulin pool for microtubule dynamics. elife. 2015;4: pubmed publisher
..Our studies suggest a revised paradigm for tubulin cofactors and Arl2 functions as a catalytic chaperone that regulates soluble Î±Î²-tubulin assembly and maintenance to support microtubule dynamics. ..
Veltel S, Gasper R, Eisenacher E, Wittinghofer A. The retinitis pigmentosa 2 gene product is a GTPase-activating protein for Arf-like 3. Nat Struct Mol Biol. 2008;15:373-80 pubmed publisher
..The cognate G protein-GAP pair is conserved in yeast as Cin4-Cin2, and the ability of RP2 to act as a GAP can be correlated with its ability to complement a CIN2-deletion phenotype.
Stearns T, Hoyt M, Botstein D. Yeast mutants sensitive to antimicrotubule drugs define three genes that affect microtubule function. Genetics. 1990;124:251-62 pubmed
..groups: TUB1, TUB2 and TUB3, the three tubulin genes of yeast, and three new genes, which we have named CIN1, CIN2 and CIN4...
Hoyt M, Macke J, Roberts B, Geiser J. Saccharomyces cerevisiae PAC2 functions with CIN1, 2 and 4 in a pathway leading to normal microtubule stability. Genetics. 1997;146:849-57 pubmed
The products of the Saccharomyces cerevisiae CIN1, CIN2 and CIN4 genes participate in a nonessential pathway required for normal microtubule function. In this article, we demonstrate that the product of PAC2 also functions in this pathway...
Fleming J, Vega L, Solomon F. Function of tubulin binding proteins in vivo. Genetics. 2000;156:69-80 pubmed
..This approach identifies four genes-CIN1, CIN2, CIN4, and PAC2-as affecting heterodimer formation in vivo...
Lee M, Spencer F. Bipolar orientation of chromosomes in Saccharomyces cerevisiae is monitored by Mad1 and Mad2, but not by Mad3. Proc Natl Acad Sci U S A. 2004;101:10655-60 pubmed
..Cells without MAD3 were checkpoint-proficient. We conclude that Mad1 and Mad2 are required to detect bipolar orientation and/or tension at kinetochores, whereas Mad3 is not. ..
Haldar S, Sarkar S, Singh V, Sinha P. High copies of SUM1 enhance the stability of wild-type microtubules against adverse conditions in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2012;418:525-30 pubmed publisher
..Therefore, we conclude that high copies of Sum1p stabilize microtubules against a variety of adverse and destabilizing conditions like mutations, low temperatures and drugs. ..