CHS2

Summary

Gene Symbol: CHS2
Description: chitin synthase CHS2
Alias: chitin synthase CHS2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Schmidt M, Bowers B, Varma A, Roh D, Cabib E. In budding yeast, contraction of the actomyosin ring and formation of the primary septum at cytokinesis depend on each other. J Cell Sci. 2002;115:293-302 pubmed
    Saccharomyces cerevisiae chs2 mutants are unable to synthesize primary septum chitin, and myo1 mutants cannot construct a functional contractile ring. The morphology of the two mutants, as observed by electron microscopy, is very similar...
  2. Martinez Rucobo F, Eckhardt Strelau L, Terwisscha van Scheltinga A. Yeast chitin synthase 2 activity is modulated by proteolysis and phosphorylation. Biochem J. 2009;417:547-54 pubmed publisher
    Saccharomyces cerevisiae Chs2 (chitin synthase 2) synthesizes the primary septum after mitosis is completed. It is essential for proper cell separation and is expected to be highly regulated...
  3. Chuang J, Schekman R. Differential trafficking and timed localization of two chitin synthase proteins, Chs2p and Chs3p. J Cell Biol. 1996;135:597-610 pubmed
    ..Collectively, these data suggest that the trafficking of Chs2p and Chs3p diverges after endocytosis; Chs3p is not delivered to the vacuole, but instead may be recycled. ..
  4. Cabib E, Schmidt M. Chitin synthase III activity, but not the chitin ring, is required for remedial septa formation in budding yeast. FEMS Microbiol Lett. 2003;224:299-305 pubmed
    ..However, in chs2 mutants CSIII becomes essential for the formation of aberrant septa...
  5. VerPlank L, Li R. Cell cycle-regulated trafficking of Chs2 controls actomyosin ring stability during cytokinesis. Mol Biol Cell. 2005;16:2529-43 pubmed
    ..This phenotype led us to identify Chs2, a transmembrane protein targeted to the bud neck through the exocytic pathway, as necessary for actomyosin ring ..
  6. Chin C, Bennett A, Ma W, Hall M, Yeong F. Dependence of Chs2 ER export on dephosphorylation by cytoplasmic Cdc14 ensures that septum formation follows mitosis. Mol Biol Cell. 2012;23:45-58 pubmed publisher
    ..In Saccharomyces cerevisiae, chitin synthase 2 (Chs2), which lays down the primary septum at the mother-daughter neck, also ensures proper actomyosin ring constriction ..
  7. Oh Y, Chang K, Orlean P, Wloka C, Deshaies R, Bi E. Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast. Mol Biol Cell. 2012;23:2445-56 pubmed publisher
    ..the primary septum (PS), a functional equivalent of animal ECM, is synthesized during cytokinesis by the chitin synthase Chs2. Here, we report that Dbf2, a conserved mitotic exit kinase, localizes to the division site after Chs2 and ..
  8. Zhang G, Kashimshetty R, Ng K, Tan H, Yeong F. Exit from mitosis triggers Chs2p transport from the endoplasmic reticulum to mother-daughter neck via the secretory pathway in budding yeast. J Cell Biol. 2006;174:207-20 pubmed
    ..The dependence of Chs2p exit from the ER followed by its transport to the neck upon mitotic exit ensures that septum formation occurs only after the completion of mitotic events. ..
  9. Foltman M, Molist I, Arcones I, Sacristan C, Filali Mouncef Y, Roncero C, et al. Ingression Progression Complexes Control Extracellular Matrix Remodelling during Cytokinesis in Budding Yeast. PLoS Genet. 2016;12:e1005864 pubmed publisher
    ..The budding yeast chitin synthase Chs2 makes the primary septum, a special layer of the ECM, which is an essential process during cell division...

More Information

Publications18

  1. Jakobsen M, Cheng Z, Lam S, Roth Johnson E, Barfield R, Schekman R. Phosphorylation of Chs2p regulates interaction with COPII. J Cell Sci. 2013;126:2151-6 pubmed publisher
    ..Our data suggest that dephosphorylation functions as a molecular switch for regulated ER exit of Chs2p. ..
  2. Onishi M, Ko N, Nishihama R, Pringle J. Distinct roles of Rho1, Cdc42, and Cyk3 in septum formation and abscission during yeast cytokinesis. J Cell Biol. 2013;202:311-29 pubmed publisher
    ..This work suggests a general role for the catalytically inactive transglutaminases of fungi and animals, some of which have previously been implicated in cytokinesis. ..
  3. Mancini Lombardi I, Palani S, Meitinger F, Darieva Z, Hofmann A, Sharrocks A, et al. Lre1 directly inhibits the NDR/Lats kinase Cbk1 at the cell division site in a phosphorylation-dependent manner. Curr Biol. 2013;23:1736-45 pubmed publisher
    ..We propose that similar inhibitory proteins may also provide fine tuning for the activity of NDR kinases in other organisms. ..
  4. Cassani C, Raspelli E, Santo N, Chiroli E, Lucchini G, Fraschini R. Saccharomyces cerevisiae Dma proteins participate in cytokinesis by controlling two different pathways. Cell Cycle. 2013;12:2794-808 pubmed publisher
    ..Altogether, these findings suggest that the Dma proteins act as negative regulators of cytokinesis. ..
  5. Yabe T, Yamada Okabe T, Nakajima T, Sudoh M, Arisawa M, Yamada Okabe H. Mutational analysis of chitin synthase 2 of Saccharomyces cerevisiae. Identification of additional amino acid residues involved in its catalytic activity. Eur J Biochem. 1998;258:941-7 pubmed
    ..Thr805, the mutation of which severely diminished enzymatic activity and the enzyme's ability to rescue the yeast chs2 delta chs3 delta null mutant strain...
  6. Oh Y, Schreiter J, Nishihama R, Wloka C, Bi E. Targeting and functional mechanisms of the cytokinesis-related F-BAR protein Hof1 during the cell cycle. Mol Biol Cell. 2013;24:1305-20 pubmed publisher
    ..role in maintaining the symmetry of Myo1 ring constriction during cytokinesis and that Hof1 interacts with Chs2, a chitin synthase that is required for primary septum formation...
  7. Chin C, Tan K, Onishi M, Chew Y, Augustine B, Lee W, et al. Timely Endocytosis of Cytokinetic Enzymes Prevents Premature Spindle Breakage during Mitotic Exit. PLoS Genet. 2016;12:e1006195 pubmed publisher
    ..spindle breakage observed in endocytosis mutants can be rescued either by deleting or inhibiting the activities of, CHS2, CHS3 and FKS1, which are involved in septum formation...
  8. Meitinger F, Palani S, Hub B, Pereira G. Dual function of the NDR-kinase Dbf2 in the regulation of the F-BAR protein Hof1 during cytokinesis. Mol Biol Cell. 2013;24:1290-304 pubmed publisher
    ..Thus our data show that Dbf2 coordinates septin and AMR functions during cytokinesis through the regulation/control of Hof1. ..
  9. Devrekanli A, Foltman M, Roncero C, Sanchez Diaz A, Labib K. Inn1 and Cyk3 regulate chitin synthase during cytokinesis in budding yeasts. J Cell Sci. 2012;125:5453-66 pubmed publisher
    ..In this way, we identified 22 dominant mutations of CHS2 (chitin synthase II) that map to two neighbouring sites in the catalytic domain...