Genomes and Genes
Gene Symbol: CHO1
Description: CDP-diacylglycerol-serine O-phosphatidyltransferase
Alias: PSS1, CDP-diacylglycerol-serine O-phosphatidyltransferase
Species: Saccharomyces cerevisiae S288c
- Yamagami K, Yamamoto T, Sakai S, Mioka T, Sano T, Igarashi Y, et al. Inositol depletion restores vesicle transport in yeast phospholipid flippase mutants. PLoS ONE. 2015;10:e0120108 pubmed publisher..These results suggest that inositol depletion changes the lipid composition of the endosomal/TGN membranes, which results in vesicle formation from these membranes in the absence of flippases. ..
- Chen S, Wang J, Muthusamy B, Liu K, Zare S, Andersen R, et al. Roles for the Drs2p-Cdc50p complex in protein transport and phosphatidylserine asymmetry of the yeast plasma membrane. Traffic. 2006;7:1503-17 pubmed
- Miyata N, Goda N, Matsuo K, Hoketsu T, Kuge O. Cooperative function of Fmp30, Mdm31, and Mdm32 in Ups1-independent cardiolipin accumulation in the yeast Saccharomyces cerevisiae. Sci Rep. 2017;7:16447 pubmed publisher..we found that the accumulation of CL in ups1? cells was enhanced by deletion of not only UPS2, but also PSD1 and CHO1 encoding a PS synthase, suggesting that low PE levels in mitochondria were relevant to the enhancement of CL ..
- Curwin A, LeBlanc M, Fairn G, McMaster C. Localization of lipid raft proteins to the plasma membrane is a major function of the phospholipid transfer protein Sec14. PLoS ONE. 2013;8:e55388 pubmed publisher..Localization of both lipid raft proteins was restored upon increased expression of the sec14(ts) allele. We suggest that a major function provided by Sec14 is trafficking and localization of lipid raft proteins. ..
- Marek M, Milles S, Schreiber G, Daleke D, Dittmar G, Herrmann A, et al. The yeast plasma membrane ATP binding cassette (ABC) transporter Aus1: purification, characterization, and the effect of lipids on its activity. J Biol Chem. 2011;286:21835-43 pubmed publisher..These results suggest a direct interaction between Aus1 and PS that is critical for the activity of the transporter...
- Janitor M, Obernauerova M, Kohlwein S, Subik J. The pel1 mutant of Saccharomyces cerevisiae is deficient in cardiolipin and does not survive the disruption of the CHO1 gene encoding phosphatidylserine synthase. FEMS Microbiol Lett. 1996;140:43-7 pubmed..Disruption of the PEL1 gene in cells containing a null mutation in the CHO1 gene was lethal...
- Carman G, Matas J. Solubilization of microsomal-associated phosphatidylserine synthase and phosphatidylinositol synthase from Saccharomyces cerevisiae. Can J Microbiol. 1981;27:1140-9 pubmed..1 mM, respectively. Thioreactive agents inhibited both enzymatic activities. Both solubilized enzymatic activities were thermally inactivated at temperatures above 30 degrees C. ..
- Nakamura H, Miura K, Fukuda Y, Shibuya I, Ohta A, Takagi M. Phosphatidylserine synthesis required for the maximal tryptophan transport activity in Saccharomyces cerevisiae. Biosci Biotechnol Biochem. 2000;64:167-72 pubmedSaccharomyces cerevisiae cho1/pss mutants, which are severely impaired in phosphatidylserine (PS) synthesis, do not have detectable amounts of PS in their lipid fractions...
- Hatakeyama R, Kono K, Yoshida S. Ypk1 and Ypk2 kinases maintain Rho1 at the plasma membrane by flippase-dependent lipid remodeling after membrane stresses. J Cell Sci. 2017;130:1169-1178 pubmed publisher..Since all the signaling molecules studied here are conserved in higher eukaryotes, our findings might represent a general mechanism to cope with PM stress. ..
- Bae Lee M, Carman G. Phosphatidylserine synthesis in Saccharomyces cerevisiae. Purification and characterization of membrane-associated phosphatidylserine synthase. J Biol Chem. 1984;259:10857-62 pubmed..Thioreactive agents inhibited enzyme activity. The enzyme was thermally labile above 40 degrees C. Results of isotopic exchange reactions between substrates and products suggest that the enzyme catalyzes a sequential Bi Bi reaction. ..
- Tani M, Kuge O. Involvement of complex sphingolipids and phosphatidylserine in endosomal trafficking in yeast Saccharomyces cerevisiae. Mol Microbiol. 2012;86:1262-80 pubmed publisher..exhibited abnormal intracellular accumulation of an exocytic v-SNARE, Snc1, under phosphatidylserine synthase gene (PSS1)-repressive conditions, although in wild-type cells, Snc1 was known to cycle between plasma membranes and the late ..
- Tani M, Kuge O. Requirement of a specific group of sphingolipid-metabolizing enzyme for growth of yeast Saccharomyces cerevisiae under impaired metabolism of glycerophospholipids. Mol Microbiol. 2010;78:395-413 pubmed..reported, SAC1-repressed cells exhibited a reduced cellular phosphatidylserine (PS) level, and overexpression of PSS1 encoding PS synthase complemented the growth defects of scs7?, csg1? and ipt1? cells under SAC1-repressive ..
- Choi H, Han G, Carman G. Phosphorylation of yeast phosphatidylserine synthase by protein kinase A: identification of Ser46 and Ser47 as major sites of phosphorylation. J Biol Chem. 2010;285:11526-36 pubmed publisherThe CHO1-encoded phosphatidylserine synthase from Saccharomyces cerevisiae is phosphorylated and inhibited by protein kinase A in vitro...