Gene Symbol: CHO1
Description: CDP-diacylglycerol-serine O-phosphatidyltransferase
Alias: PSS1, CDP-diacylglycerol-serine O-phosphatidyltransferase
Species: Saccharomyces cerevisiae S288c
Products:     CHO1

Top Publications

  1. Takeda M, Yamagami K, Tanaka K. Role of phosphatidylserine in phospholipid flippase-mediated vesicle transport in Saccharomyces cerevisiae. Eukaryot Cell. 2014;13:363-75 pubmed publisher
    ..We show here that overexpression of the phosphatidylserine synthase gene CHO1 suppresses defects in the endocytic recycling pathway in flippase mutants...
  2. Kuchler K, Daum G, Paltauf F. Subcellular and submitochondrial localization of phospholipid-synthesizing enzymes in Saccharomyces cerevisiae. J Bacteriol. 1986;165:901-10 pubmed
    ..Data concerning susceptibility to various inhibitors, heat sensitivity, and the pH optima indicate that there is a close similarity of the mitochondrial and microsomal enzymes that catalyze the same reaction. ..
  3. Choi H, Carman G. Respiratory deficiency mediates the regulation of CHO1-encoded phosphatidylserine synthase by mRNA stability in Saccharomyces cerevisiae. J Biol Chem. 2007;282:31217-27 pubmed
    The CHO1-encoded phosphatidylserine synthase (CDP-diacylglycerol:l-serine O-phosphatidyltransferase, EC is one of the most highly regulated phospholipid biosynthetic enzymes in the yeast Saccharomyces cerevisiae...
  4. Zinser E, Sperka Gottlieb C, Fasch E, Kohlwein S, Paltauf F, Daum G. Phospholipid synthesis and lipid composition of subcellular membranes in the unicellular eukaryote Saccharomyces cerevisiae. J Bacteriol. 1991;173:2026-34 pubmed
    ..The rigidity of the plasma membrane and of secretory vesicles, determined by measuring fluorescence anisotropy by using trimethylammonium diphenylhexatriene as a probe, can be attributed to the high content of ergosterol. ..
  5. Li Z, Haase E, Brendel M. Hyper-resistance to nitrogen mustard in Saccharomyces cerevisiae is caused by defective choline transport. Curr Genet. 1991;19:423-7 pubmed
    ..Gene disruption of HNM1 revealed that this gene is non-essential for cells prototrophic for choline (CHO1) but lethal for cells with a cho1 genotype...
  6. Han G, Carman G. Yeast PAH1-encoded phosphatidate phosphatase controls the expression of CHO1-encoded phosphatidylserine synthase for membrane phospholipid synthesis. J Biol Chem. 2017;292:13230-13242 pubmed publisher
    ..Here, we examined the PAP-mediated regulation of CHO1-encoded phosphatidylserine synthase (PSS), which catalyzes the committed step for the synthesis of major ..
  7. Atkinson K, Jensen B, Kolat A, Storm E, Henry S, Fogel S. Yeast mutants auxotrophic for choline or ethanolamine. J Bacteriol. 1980;141:558-64 pubmed
    ..The mutants map to a single locus (cho1) on chromosome V...
  8. Kannan M, Lahiri S, Liu L, Choudhary V, Prinz W. Phosphatidylserine synthesis at membrane contact sites promotes its transport out of the ER. J Lipid Res. 2017;58:553-562 pubmed publisher
    ..Together, these findings indicate that PS production at MCSs promotes PS transport out of the ER and suggest that phospholipid production at MCSs may be a general mechanism of channeling lipids to specific cellular compartments. ..
  9. Mandala S, Thornton R, Tu Z, Kurtz M, Nickels J, Broach J, et al. Sphingoid base 1-phosphate phosphatase: a key regulator of sphingolipid metabolism and stress response. Proc Natl Acad Sci U S A. 1998;95:150-5 pubmed

More Information


  1. Yamagami K, Yamamoto T, Sakai S, Mioka T, Sano T, Igarashi Y, et al. Inositol depletion restores vesicle transport in yeast phospholipid flippase mutants. PLoS ONE. 2015;10:e0120108 pubmed publisher
    ..These results suggest that inositol depletion changes the lipid composition of the endosomal/TGN membranes, which results in vesicle formation from these membranes in the absence of flippases. ..
  2. Chen S, Wang J, Muthusamy B, Liu K, Zare S, Andersen R, et al. Roles for the Drs2p-Cdc50p complex in protein transport and phosphatidylserine asymmetry of the yeast plasma membrane. Traffic. 2006;7:1503-17 pubmed
  3. Miyata N, Goda N, Matsuo K, Hoketsu T, Kuge O. Cooperative function of Fmp30, Mdm31, and Mdm32 in Ups1-independent cardiolipin accumulation in the yeast Saccharomyces cerevisiae. Sci Rep. 2017;7:16447 pubmed publisher
    ..we found that the accumulation of CL in ups1? cells was enhanced by deletion of not only UPS2, but also PSD1 and CHO1 encoding a PS synthase, suggesting that low PE levels in mitochondria were relevant to the enhancement of CL ..
  4. Curwin A, LeBlanc M, Fairn G, McMaster C. Localization of lipid raft proteins to the plasma membrane is a major function of the phospholipid transfer protein Sec14. PLoS ONE. 2013;8:e55388 pubmed publisher
    ..Localization of both lipid raft proteins was restored upon increased expression of the sec14(ts) allele. We suggest that a major function provided by Sec14 is trafficking and localization of lipid raft proteins. ..
  5. Marek M, Milles S, Schreiber G, Daleke D, Dittmar G, Herrmann A, et al. The yeast plasma membrane ATP binding cassette (ABC) transporter Aus1: purification, characterization, and the effect of lipids on its activity. J Biol Chem. 2011;286:21835-43 pubmed publisher
    ..These results suggest a direct interaction between Aus1 and PS that is critical for the activity of the transporter...
  6. Janitor M, Obernauerova M, Kohlwein S, Subik J. The pel1 mutant of Saccharomyces cerevisiae is deficient in cardiolipin and does not survive the disruption of the CHO1 gene encoding phosphatidylserine synthase. FEMS Microbiol Lett. 1996;140:43-7 pubmed
    ..Disruption of the PEL1 gene in cells containing a null mutation in the CHO1 gene was lethal...
  7. Carman G, Matas J. Solubilization of microsomal-associated phosphatidylserine synthase and phosphatidylinositol synthase from Saccharomyces cerevisiae. Can J Microbiol. 1981;27:1140-9 pubmed
    ..1 mM, respectively. Thioreactive agents inhibited both enzymatic activities. Both solubilized enzymatic activities were thermally inactivated at temperatures above 30 degrees C. ..
  8. Nakamura H, Miura K, Fukuda Y, Shibuya I, Ohta A, Takagi M. Phosphatidylserine synthesis required for the maximal tryptophan transport activity in Saccharomyces cerevisiae. Biosci Biotechnol Biochem. 2000;64:167-72 pubmed
    Saccharomyces cerevisiae cho1/pss mutants, which are severely impaired in phosphatidylserine (PS) synthesis, do not have detectable amounts of PS in their lipid fractions...
  9. Hatakeyama R, Kono K, Yoshida S. Ypk1 and Ypk2 kinases maintain Rho1 at the plasma membrane by flippase-dependent lipid remodeling after membrane stresses. J Cell Sci. 2017;130:1169-1178 pubmed publisher
    ..Since all the signaling molecules studied here are conserved in higher eukaryotes, our findings might represent a general mechanism to cope with PM stress. ..
  10. Bae Lee M, Carman G. Phosphatidylserine synthesis in Saccharomyces cerevisiae. Purification and characterization of membrane-associated phosphatidylserine synthase. J Biol Chem. 1984;259:10857-62 pubmed
    ..Thioreactive agents inhibited enzyme activity. The enzyme was thermally labile above 40 degrees C. Results of isotopic exchange reactions between substrates and products suggest that the enzyme catalyzes a sequential Bi Bi reaction. ..
  11. Tani M, Kuge O. Involvement of complex sphingolipids and phosphatidylserine in endosomal trafficking in yeast Saccharomyces cerevisiae. Mol Microbiol. 2012;86:1262-80 pubmed publisher
    ..exhibited abnormal intracellular accumulation of an exocytic v-SNARE, Snc1, under phosphatidylserine synthase gene (PSS1)-repressive conditions, although in wild-type cells, Snc1 was known to cycle between plasma membranes and the late ..
  12. Tani M, Kuge O. Requirement of a specific group of sphingolipid-metabolizing enzyme for growth of yeast Saccharomyces cerevisiae under impaired metabolism of glycerophospholipids. Mol Microbiol. 2010;78:395-413 pubmed
    ..reported, SAC1-repressed cells exhibited a reduced cellular phosphatidylserine (PS) level, and overexpression of PSS1 encoding PS synthase complemented the growth defects of scs7?, csg1? and ipt1? cells under SAC1-repressive ..
  13. Choi H, Han G, Carman G. Phosphorylation of yeast phosphatidylserine synthase by protein kinase A: identification of Ser46 and Ser47 as major sites of phosphorylation. J Biol Chem. 2010;285:11526-36 pubmed publisher
    The CHO1-encoded phosphatidylserine synthase from Saccharomyces cerevisiae is phosphorylated and inhibited by protein kinase A in vitro...